Attch. 23 LAB SAMPLING QTY.xlsx
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- Solicitation number
- 191BWC26R0009
About this file
This is a laboratory sampling and analysis requirements document for the Southbay Wastewater Treatment Plant (SBIWTP) Operations and Maintenance contract. The document specifies comprehensive water quality testing protocols across multiple sampling streams, including influent, effluent, stormwater, biosolids, transboundary flows, and wet weather events, with detailed frequency schedules and analytical methods.
The sampling requirements are organized by water stream category and testing frequency. Influent and effluent sampling require 365 annual samples for standard parameters such as biochemical oxygen demand, chemical oxygen demand, pH, suspended solids, and turbidity using Standard Methods (SM) and EPA protocols. Both streams require 52 annual samples for metals analysis via ICP EPA 200.7 and 12 annual samples for nutrient analyses including ammonia, nitrate, and phosphate. Specialized testing occurs quarterly (4 samples annually) for dioxin, pesticides, and semivolatile organics. Stormwater analysis includes 4 annual samples across similar parameters. Biosolids testing requires 12 monthly samples for metals analysis using EPA 6010B and STLC extraction methods, plus daily pH and total solids measurements (365 samples annually). Transboundary flows and wet weather sampling at the Canyon Collector require 20 samples annually each for comprehensive analysis including dioxin, asbestos, pesticides, metals, and microbial indicators. Primary and secondary effluent sampling mirrors influent/effluent protocols with 365 daily samples for routine parameters, 52 samples for metals and extractable materials, and 12 quarterly samples for nutrients and microbial testing. All analyses reference specific EPA and Standard Methods protocols with indicated turnaround times, primarily 5-day analysis windows.
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Influent Sampling
| Analysis/ Service | Method | Samples per Year |
| Biochemical Oxygen Demand by SM 5210 B | SM 5210 B | 365 |
| Carbonaceous Biochemical Oxygen Demand SM 5210B | SM 5210B | 365 |
| Chemical Oxygen Demand by HACH 8000 | HACH 8000 | 365 |
| Floatables by SM 2530B | SM 2530B | 365 |
| pH by SM 4500-H+ | SM 4500-H+ | 365 |
| Specific Conductance (EC) by SM 2510 B | SM 2510 B | 365 |
| Temperature | Field Test | 365 |
| Total Dissolved Solids by SM 2540 C | SM 2540 C | 365 |
| Total Settleable Solids by SM 2540 F | SM 2540 F | 365 |
| Total Suspended Solids by SM 2540 D | SM 2540 D | 365 |
| Turbidity by SM 2130B | SM 2130 B | 365 |
| Volatile Solids | SM 2540 E | 365 |
| Chlorine T- Field Test | Test Kit | 365 |
| Dissolved Oxygen by SM 4500-0 G | SM 4500-0 G | 52 |
| Hexane Extractable Material | EPA 1664B | 52 |
| pH by Field Test | Field Test | 52 |
| Hexavalent Chromium by EPA 218.6 | EPA 218.6 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Mercury | total by CVAA | 52 |
| Cyanide by EPA 335.4 | EPA 335.4 | 52 |
| Alkalinity by SM-2320B | SM 2320 B | 12 |
| Ammonia as N | SM 4500-NH3 B/C | 12 |
| Nitrate as N by HACH 10206 | HACH 10206 | 12 |
| Nitrite-NO2/N HACH 8507 | HACH 8507 | 12 |
| Nitrogen | Total by Calculation | 12 |
| Orthophosphate as P by SM 4500-P E | SM 4500-P E | 12 |
| Salinity by SM 2520 | SM 2520 | 12 |
| SM4500-NH3 C | Calculation | 12 |
| Suspended Solids by SM 2540 D Total | SM 2540 D | 12 |
| Dissolved Inorganic Carbon by SM 5310 B | SM 5310 B | 12 |
| Individual dissolved metal by ICP | EPA 200.7 | 12 |
| Individual total metal by ICP | EPA 200.7 | 12 |
| Individual total metal by ICP | EPA 200.7 | 12 |
| Individual total metal by ICP | EPA 200.7 | 12 |
| Pesticides & PCBs in Liquid by GC/ECD | EPA 608.3 | 12 |
| Semivolatile Organics by GC/MS | EPA 625.1 | 12 |
| SM4500-P E | SM 4500-P E | 12 |
| TOC | SM 5310 C | 12 |
| Individual dissolved metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Pesticides & PCBs in Liquid by GC/ECD | EPA 608.3 | 4 |
| Semivolatile Organics by GC/MS | EPA 625.1 | 4 |
| Dioxin (2,3,7,8-TCDD) by EPA 8280 | EPA 8280 | 4 |
| Tributyltin | NA | 4 |
| Volatile Organics in Liquid by GCMS/ | EPA 624.1 | 4 |
Effluent Sampling
| Analysis/ Service | Method | Samples per Year |
| Biochemical Oxygen Demand by SM 5210 B | SM 5210 B | 365 |
| Carbonaceous Biochemical Oxygen Demand SM 5210B | SM 5210B | 365 |
| Chemical Oxygen Demand by HACH 8000 | HACH 8000 | 365 |
| Floatables by SM 2530B | SM 2530B | 365 |
| pH by SM 4500-H+ | SM 4500-H+ | 365 |
| Specific Conductance (EC) by SM 2510 B | SM 2510 B | 365 |
| Temperature | Field Test | 365 |
| Total Dissolved Solids by SM 2540 C | SM 2540 C | 365 |
| Total Settleable Solids by SM 2540 F | SM 2540 F | 365 |
| Total Suspended Solids by SM 2540 D | SM 2540 D | 365 |
| Turbidity by SM 2130B | SM 2130 B | 365 |
| Volatile Solids | SM 2540 E | 365 |
| Chlorine T- Field Test | Test Kit | 365 |
| Dissolved Oxygen by SM 4500-0 G | SM 4500-0 G | 52 |
| Hexane Extractable Material | EPA 1664B | 52 |
| pH by Field Test | Field Test | 52 |
| Hexavalent Chromium by EPA 218.6 | EPA 218.6 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Individual total metal by ICP | EPA 200.7 | 52 |
| Mercury | total by CVAA | 52 |
| Cyanide by EPA 335.4 | EPA 335.4 | 52 |
| Alkalinity by SM-2320B | SM 2320 B | 12 |
| Ammonia as N | SM 4500-NH3 B/C | 12 |
| Nitrate as N by HACH 10206 | HACH 10206 | 12 |
| Nitrite-NO2/N HACH 8507 | HACH 8507 | 12 |
| Nitrogen | Total by Calculation | 12 |
| Orthophosphate as P by SM 4500-P E | SM 4500-P E | 12 |
| Salinity by SM 2520 | SM 2520 | 12 |
| SM4500-NH3 C | Calculation | 12 |
| Suspended Solids by SM 2540 D Total | SM 2540 D | 12 |
| Dissolved Inorganic Carbon by SM 5310 B | SM 5310 B | 12 |
| Individual dissolved metal by ICP | EPA 200.7 | 12 |
| Individual total metal by ICP | EPA 200.7 | 12 |
| Individual total metal by ICP | EPA 200.7 | 12 |
| Enterococcus by SM 9230B (MPN/Tubes) | SM 9230B | 12 |
| Fecal Coliform by SM 9222D (Membrane Filtration) | SM 9222D | 12 |
| Total Coliform by SM 9222B (Membrane Filtration) | SM 9222B | 12 |
| Pesticides & PCBs in Liquid by GC/ECD | EPA 608.3 | 12 |
| Semivolatile Organics by GC/MS | EPA 625.1 | 12 |
| Gross alpha | EPA 900.0 | 12 |
| Gross beta | EPA 900.0 | 12 |
| SM4500-P E | SM 4500-P E | 12 |
| TOC | SM 5310 C | 12 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Dioxin (2,3,7,8-TCDD) by EPA 8280 | EPA 8280 | 4 |
| Tributyltin | NA | 4 |
| Volatile Organics in Liquid by GCMS/ | EPA 624.1 | 4 |
| Chronic Toxicity | 52 |
Stormwater
| Analysis/ Service | Method | Samples per Year |
| Ammonia as N | SM 4500-NH3 B/C | 4 |
| Biochemical Oxygen Demand by SM 5210 B | SM 5210 B | 4 |
| Chemical Oxygen Demand by HACH 8000 | HACH 8000 | 4 |
| Dissolved Inorganic Carbon by SM 5310 B | SM 5310 B | 4 |
| Dissolved Oxygen by SM 4500-0 G | SM 4500-0 G | 4 |
| Hexane Extractable Material | EPA 1664B | 4 |
| Individual dissolved metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Individual total metal by ICP | EPA 200.7 | 4 |
| Nitrate as N by HACH 10206 | HACH 10206 | 4 |
| Nitrite-NO2/N HACH 8507 | HACH 8507 | 4 |
| Orthophosphate as P by SM 4500-P E | SM 4500-P E | 4 |
| Pesticides & PCBs in Liquid by GC/ECD | EPA 608.3 | 4 |
| pH by SM 4500-H+ | SM 4500-H+ | 4 |
| Salinity by SM 2520 | SM 2520 | 4 |
| Total Suspended Solids by SM 2540 D | SM 2540 D | 4 |
Biosolids
| Analysis/ Service | Samples per Year |
| Hg STLC CVAA 7470 [5 day] | 12 |
| K Total 6010B-dry weight ICP [5 day] | 12 |
| Li Total 6010B-dry weight ICP [5 day] | 12 |
| Mg Total 6010B-dry weight ICP [5 day] | 12 |
| Ni STLC 6010B ICP [5 day] | 12 |
| Pb STLC 6010B ICP [5 day] | 12 |
| Se STLC 6010B ICP [5 day] | 12 |
| Solids Dry Weight [5 day] | 12 |
| STLC-Extraction-Met [5 day] | 12 |
| T-22-6010B-dry weight [5 day] | 12 |
| TVS-SM2540G [5 day] | 12 |
| V STLC 6010B ICP [5 day] | 12 |
| Zn STLC 6010B ICP [5 day] | 12 |
| Ba STLC 6010B ICP [5 day] | 12 |
| Ca Total 6010B-dry weight ICP [5 day] | 12 |
| Cd STLC 6010B ICP [5 day] | 12 |
| Cr STLC 6010B ICP [5 day] | 12 |
| Cu STLC 6010B ICP [5 day] | 12 |
| pH EPA 9045C Soil [5 day] | 365 |
| Total Solids-SM2540G [5 day] | 365 |
Transboundary Flows
| Analysis/Description | Anticipated Number of Samples per Year |
| SUB Dioxin 8280 (2378-TCDD) [5 day] Liquid | 20 |
| SUB Asbestos (TEM) [5 day] Liquid | 20 |
| 608-Veolia [5 day] Liquid | 20 |
| 624-Veolia [5 day] Liquid | 20 |
| 625-Veolia [5 day] Liquid | 20 |
| BOD-SM 5210 B [5 day] Liquid | 20 |
| Diss Oxygen SM 4500-0 G [5 day] Liquid | 20 |
| Enterococcus 9230B (MPN/Tubes) [5 day] Liquid | 20 |
| Fecal Coliform 9221E (MPN/Tubes) [5 day] Liquid | 20 |
| Hg Total CVAA 245.1 [5 day] Liquid | 20 |
| MBAS-SM 5540C [5 day] Liquid | 20 |
| pH SM 4500-H+ Water [5 day] Liquid | 20 |
| Phosphorous-SM 4500-P E [5 day] Liquid | 20 |
| Conductivity | 20 |
| Total Nitrogen-SM4500-N [5 day] Liquid | 20 |
| TSS-SM2540D [5 day] Liquid | 20 |
| Turbidity SM2130B [5 day] Liquid | 20 |
| SUB 8141A Organo-Phosphorous Pesticides (5 day) Liquid | 20 |
| SUB EPA 335.4-Cyanide [5 day] Liquid | 20 |
| Metals by EPA 200.7 | 20 |
| Temperature | 20 |
| Ammonia | 20 |
CC Wet Weather Sampling Canyon Collector Wet Weather Event Sampling
| Analysis/Description | Anticipated Number of Sample Sets per Year |
| SUB Dioxin 8280 (2378-TCDD) [5 day] Liquid | 20 |
| SUB Asbestos (TEM) [5 day] Liquid | 20 |
| 608-Veolia [5 day] Liquid | 20 |
| 624-Veolia [5 day] Liquid | 20 |
| 625-Veolia [5 day] Liquid | 20 |
| BOD-SM 5210 B [5 day] Liquid | 20 |
| Diss Oxygen SM 4500-0 G [5 day] Liquid | 20 |
| Enterococcus 9230B (MPN/Tubes) [5 day] Liquid | 20 |
| Fecal Coliform 9221E (MPN/Tubes) [5 day] Liquid | 20 |
| Hg Total CVAA 245.1 [5 day] Liquid | 20 |
| MBAS-SM 5540C [5 day] Liquid | 20 |
| pH SM 4500-H+ Water [5 day] Liquid | 20 |
| Phosphorous-SM 4500-P E [5 day] Liquid | 20 |
| TDS-SM2540 C [5 day] Liquid | 20 |
| Total Nitrogen-SM4500-N [5 day] Liquid | 20 |
| TSS-SM2540D [5 day] Liquid | 20 |
| Turbidity SM2130B [5 day] Liquid | 20 |
| SUB 8141A Organo-Phosphorous Pesticides (5 day) Liquid | 20 |
| SUB EPA 335.4-Cyanide [5 day] Liquid | 20 |
| Metals by EPA 200.7 | 20 |
| Temperature | 20 |
| Ammonia | 20 |
Primary Effluent Sampling
| IBWC 10 MGD Additional Lab Analysis (Primary Effluent) | |
| Analysis/ Service | Samples per Year |
| Biochemical Oxygen Demand by SM 5210 B | 365 |
| Carbonaceous Biochemical Oxygen Demand SM 5210B | 365 |
| Chemical Oxygen Demand by HACH 8000 | 365 |
| Floatables by SM 2530B | 365 |
| pH by SM 4500-H+ | 365 |
| Specific Conductance (EC) by SM 2510 B | 365 |
| Total Dissolved Solids by SM 2540 C | 365 |
| Total Settleable Solids by SM 2540 F | 365 |
| Total Suspended Solids by SM 2540 D | 365 |
| Turbidity by SM 2130B | 365 |
| Volatile Solids | 365 |
| Dissolved Oxygen by SM 4500-0 G | 52 |
| Hexane Extractable Material | 52 |
| pH by Field Test | 52 |
| Hexavalent Chromium by EPA 218.6 | 52 |
| Individual total metal by ICP - Cd | 52 |
| Individual total metal by ICP - Cr | 52 |
| Individual total metal by ICP - Pb | 52 |
| Individual total metal by ICP - Ni | 52 |
| Individual total metal by ICP - Se | 52 |
| Individual total metal by ICP - Ag | 52 |
| Individual total metal by ICP - Zn | 52 |
| Individual total metal by ICP - Cu | 52 |
| Individual total metal by ICP - Hg | 52 |
| Cyanide by EPA 335.4 | 52 |
| Alkalinity by SM-2320B | 12 |
| Ammonia as N | 12 |
| Nitrate as N by HACH 10206 | 12 |
| Nitrite-NO2/N HACH 8507 | 12 |
| Nitrogen | 12 |
| Orthophosphate as P by SM 4500-P E | 12 |
| Salinity by SM 2520 | 12 |
| SM4500-NH3 C | 12 |
| Suspended Solids by SM 2540 D Total | 12 |
| Dissolved Inorganic Carbon by SM 5310 B | 12 |
| Individual dissolved metal by ICP | 12 |
| Enterococcus by SM 9230B (MPN/Tubes) | 12 |
| Fecal Coliform by SM 9222D (Membrane Filtration) | 12 |
| Total Coliform by SM 9222B (Membrane Filtration) | 12 |
| Pesticides & PCBs in Liquid by GC/ECD | 12 |
| Semivolatile Organics by GC/MS | 12 |
| Gross alpha | 12 |
| Gross beta | 12 |
| SM4500-P E | 12 |
| TOC | 12 |
| Dioxin (2,3,7,8-TCDD) by EPA 8280 | 4 |
| Tributyltin | 4 |
| Volatile Organics in Liquid by GCMS/ | 4 |
Secondary Effluent
| IBWC 10 MGD Additional Lab Analysis (Secondary Effluent) | |
| Analysis/ Service | Samples per Year |
| Biochemical Oxygen Demand by SM 5210 B | 365 |
| Carbonaceous Biochemical Oxygen Demand SM 5210B | 365 |
| Chemical Oxygen Demand by HACH 8000 | 365 |
| Floatables by SM 2530B | 365 |
| pH by SM 4500-H+ | 365 |
| Specific Conductance (EC) by SM 2510 B | 365 |
| Total Dissolved Solids by SM 2540 C | 365 |
| Total Settleable Solids by SM 2540 F | 365 |
| Total Suspended Solids by SM 2540 D | 365 |
| Turbidity by SM 2130B | 365 |
| Volatile Solids | 365 |
| Dissolved Oxygen by SM 4500-0 G | 52 |
| Hexane Extractable Material | 52 |
| pH by Field Test | 52 |
| Hexavalent Chromium by EPA 218.6 | 52 |
| Individual total metal by ICP - Cd | 52 |
| Individual total metal by ICP - Cr | 52 |
| Individual total metal by ICP - Pb | 52 |
| Individual total metal by ICP - Ni | 52 |
| Individual total metal by ICP - Se | 52 |
| Individual total metal by ICP - Ag | 52 |
| Individual total metal by ICP - Zn | 52 |
| Individual total metal by ICP - Cu | 52 |
| Individual total metal by ICP - Hg | 52 |
| Cyanide by EPA 335.4 | 52 |
| Alkalinity by SM-2320B | 12 |
| Ammonia as N | 12 |
| Nitrate as N by HACH 10206 | 12 |
| Nitrite-NO2/N HACH 8507 | 12 |
| Nitrogen | 12 |
| Orthophosphate as P by SM 4500-P E | 12 |
| Salinity by SM 2520 | 12 |
| SM4500-NH3 C | 12 |
| Suspended Solids by SM 2540 D Total | 12 |
| Dissolved Inorganic Carbon by SM 5310 B | 12 |
| Individual dissolved metal by ICP | 12 |
| Enterococcus by SM 9230B (MPN/Tubes) | 12 |
| Fecal Coliform by SM 9222D (Membrane Filtration) | 12 |
| Total Coliform by SM 9222B (Membrane Filtration) | 12 |
| Pesticides & PCBs in Liquid by GC/ECD | 12 |
| Semivolatile Organics by GC/MS | 12 |
| Gross alpha | 12 |
| Gross beta | 12 |
| SM4500-P E | 12 |
| TOC | 12 |
| Dioxin (2,3,7,8-TCDD) by EPA 8280 | 4 |
| Tributyltin | 4 |
| Volatile Organics in Liquid by GCMS/ | 4 |
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