EXHIBIT B1_EQUIPMENT VIBRATION STANDARDS (1).pdf

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General Services Administration (GSA) seeks to lease office and lab space in Seattle, WA
Solicitation number
0WA2313
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This document provides equipment vibration standards and related costs for various pieces of sensitive laboratory equipment located across multiple labs at a research facility. The labs contain microscopes, microbalances, plate readers, sequencers, and other equipment requiring low vibration environments. Standards range from 48 to 66 VdB depending on the equipment's sensitivity and magnification level. Failure to meet the standards could result in measurement errors, failed experiments, required realignment or recalibration, and costs ranging from $150 to $7,000 depending on the specific piece of equipment and level of impact. The General Services Administration (GSA) solicitation seeks to lease nearby office and lab space in Seattle, Washington to relocate the research facility and ensure vibration levels are appropriately addressed for all sensitive equipment.

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Text version

Location Current Room

Equipment Description

Working Vibration Limit

Cost if failure or delay during operation

EFS - MMT –

Imagining (Scanning Electron Microscopy) Lab

108A Confocal microscope

48 to 54 VdB

(VC-C/VC-D)

Cost of loss:

$320/day in supplies + labor

EFS - MMT –

Imagining (Scanning Electron Microscopy) Lab

108A Scanning Electron Microscope (JEOL 65x0

MP)

55 VdB at 32 Hz (VC-C)

FE - Ecosystem Analysis – Analysis Lab B(Isotope Analysis & EFS Env.

Chem)

209 Sartorius

CP2P

microbalance

66 VdB (VC-A) for balances to 1 ug

CB – Conservation Biology – Microscopy Lab

225 Sartorius M2P microbalance

66 VdB (VC-A) for balances to 1 ug

CB – Conservation Biology – Microscopy Lab

225 Otholith analysis workstation (microscope, camera, and computer):

Zeiss Axioskop 40 with Leica

DFC 450

camera

Vibration will impact analysis using 40x optical (from vendor).

Imaging problems

EFS – Ecotoxicology

– Imagining (Microscope) Lab

213 3 Nikon SMZ dissecting microscopes;

Nikon compound microscope with epifluorescent light source

60 VdB ( to 1000x, VC-B)

Cost of loss: $980/3-day experiment in supplies + labor

EFS –

Environmental Chemistry – ASE #1, Sample Prep/Lactroscan Lipids Lab

236 1 Mettler Toledo AL204 balance;

readability 0.0001g

62 VdB for balances to

0.1 ug

EFS –

Environmental Chemistry – ASE #2, 253 1 Mettler

AJ100

balance;

62 VdB) for balances to

0.1 ug

Exhibit B1

Marine Mammal Sample Prep readability 0.0001g

EFS –

Environmental Physiology –

DNA/RNA

(Molecular Bio RNA Work) Lab

301 Mettler UM3 (readability to down to

0.0001 mg )

62 VdB for balances to

0.1 ug

EFS –

Environmental Physiology – Analysis (Hormone/Enzyme Assays) Lab

311 Molecular Devices Spectra Max 190 (96 well microplate reader)

No published limit. Assume 60 VdB (VC-B) is protective.

Cost of loss: $500 per run (reagents, scientist time). Cost to re-align machine: $1,500

EFS –

Environmental Physiology – Analysis (Hormone/Enzyme Assays) Lab

311 Perkin Elmer Victor3 1420 Multilabel counter (fluorescence plate reader)

No published limit. Assume 60 VdB (VC-B) is protective

Cost to realign/service machine $2000, Cost of failed plate reading (96 samples;

reagents and tech time) $1500

EFS –

Environmental Physiology – Analysis (Hormone/Enzyme Assays) Lab

311 Perkin Elmer Janus Automated Workstation

No published limit. Assume 60 VdB (VC-B) is protective

Cost to realign/service machine $2000, Cost of failed plate reading (96 samples;

reagents and tech time) $1500

CB – Genetics – Post Amplification PCR Lab

337 Illumina MiSeq high throughput sequencer

No published limit. Assume 60 VdB (VC- B).

Cost of loss: minimum

$2,050 per run

(reagents, scientist time); possible total loss of sample.

CB – Genetics – Post Amplification PCR Lab

337 ABI 3500

Sequencer

No published limit, environmental specification is low-vibration;

assume 60 VdB (VC-B) as protective.

CB – Genetics – Post Amplification PCR Lab

337 ABI 7900 Real- Time PCR

No published limit, environmental specification is low-vibration;

assume 60 VdB (VC-B) as protective.

EFS – Ecotoxicology

– Molecular lab

354 ABI Via7 Real-time qPCR

No published limit. Assume 60 VdB (VC- B).

Cost of loss:

$770/day in supplies + labor

4th Floor 424 Stratagene Mx3005Pro qPCR with laser detection system

No published limit, environmental specification is low-vibration;

assume 60 VdB (VC-B) as protective.

Cost of loss:$1,000 per run (reagents, scientist time); possible total loss of sample. Cost to re-align machine:

$4,400 + $500 qualification plate

CB – Genetics – Post Amplification PCR Lab

453 Stratagene Mx3000P qPCR thermocycler

Assume 60 VdB (VC-B), likely 66 VdB (VC-A) is sufficient

Cost of loss:$1,000 per run (reagents, scientist time); possible total loss of sample. Cost to re-align machine:

$4,400 + $500 qualification plate

EFS –

Environmental Physiology – Microscopy (Instrument) Lab

439 2 Nikon Eclipse compound microscopes:

400x and 1000x

60 to 66 VdB (400 to 1000x

,VC-B/VC-A)

Cost of loss:

$320/day in supplies + labor

EFS - MMT –

Extraction (Phytoplankton Culture) Lab

441 Inverted Light Microscope, Zeiss Axiovert.

Upright Zeiss Light Microscope.

60 to 66 VdB (400 to 1000x

,VC-B/VC-A)

CB – Eco Science – IMNESE & Marine Mammal Analytical Lab 447

Environmental Sample Processor, 2 robotic instruments

No published limit. Assume 60 VdB (VC- B).

Cost of lost analysis:

$550 (reagents, scientist time). Cost of re-aligning: $7,000

(technician must fly from Massachusetts)

CB – Genetics – Post Amplification PCR Lab 453

Mettler AE200, readable down to 0.0001g

62 VdB for balances to

0.1 ug

Cost of incorrect measurement: $350 -

$1.000, depending on process. Possible destruction of sample.

Cost to re-align: $150

CB – Genetics – Post Amplification PCR Lab 453

Microplate reader, ELx808IU, optical

No published limit. Assume 60 VdB (VC-B) is protective.

Cost of loss: $500 per run (reagents, scientist time). Cost to re-align machine: $2,000 +

$1,500 validation plate

CB – Genetics – Post Amplification PCR Lab 453

Microplate reader, FLx800, optical

No published limit. Assume 60 VdB (VC-B) is protective.

Cost of loss: $350 per run (reagents, scientist time). Cost to re-align machine: $2,000 +

$1,500 validation plate CB – Eco Science – IMNESE & Marine Mammal Analytical Lab 447

Benchtop processor for

ESP

Assume 60 VdB (VC-B) is protective.

Cost of loss: $150 per run (reagents, scientist time). Cost to re-align machine: $2,500

EFS –

Environmental Physiology –

DNA/RNA

(Molecular Bio RNA Work) Lab 456

Molecular Devices Versamax plate reader

No published limit. Assume 60 VdB (VC-B) is protective.

Cost of loss: $500 per run (reagents, scientist time). Cost to re-align machine: $1,500

EFS –

Environmental Physiology –

DNA/RNA

(Molecular Bio RNA Work) Lab 456

Mettler Toledo AG104 (0.1 mg readability)

62 VdB for balances to

0.1 ug

Cost of incorrect measurement: $350 -

$1.000, depending on process. Possible destruction of sample.

Cost to re-align: $150

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