21R1337_AMD5.pdf

RFP No. N6274221R1337 Amendment No. 0005

SECTION SF 30 BLOCK 14 CONTINUATION PAGE

PART 3: PROJECT PROGRAM

CHAPTER 5 – ROOM REQUIREMENTS

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CHAPTER 6 – ENGINEERING SYSTEM REQUIREMENTS

B20 EXTERIOR ENCLOSURE

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PART 6: ATTACHMENTS

ATTACHMENT A: PART A DRAWINGS

The following drawings, bearing the revision date of 09/13/2021, supersede previously issued drawings bearing the same number and title:

DRAWING

NUMBER

NAVFAC

DRAWING

NUMBER

REVISION

NUMBER DRAWING TITLE

S-002 17240925 5 ABBREVIATIONS

S-702 17240938 5 DOOR ELEVATIONS

S-703 17240939 5 DOOR SECTIONS

S-705 17240941 5 INTERNAL LOCKING DEVICES

ATTACHMENT I: NATURAL RESOURCES PERFORMANCE WORK STATEMENT

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Appendix B

Pages 26-52

Amendment No.0005

FY22 MCAF PROJECT P-1366 / AJJY073105P2 APR - Munitions Storage Igloos, Phase 2 Andersen Air Force Base, Guam

1181839 1571341

AMENDMENT No. 0005

REPLACEMENT SHEETS

CHAPTER 5: ROOM REQUIREMENTS

FY22 MCAF PROJECT P-1366 / AJJY073105P2 APR - Munitions Storage Igloos, Phase 2 1181839

PART 3 - CHAPTER 5 - Page 1

5.0 ROOM REQUIREMENTS

Room 101 – Munition Storage Magazines (MSM)

Space Characteristics

Function/adjacencies:

Special Dimensions:

Ideal Plan Dimensions: 25’-0” by 80’-0” Clear Interior Dimensions Minimum Ceiling Height: 11’-0” Clear Interior Ceiling Height

Acoustics: N/A

Access: Access from exterior via large double door

Number of Occupants: Unoccupied space

Other/Special Requirements: Design of the MSM roof slab, walls, retaining walls, foundations, floor slab, and blast doors must be in accordance with the Part A Drawings included in Attachment A of Part 6. All other components must comply with the requirements of this RFP.

Uniformat

Section Description Qty Size Specific Requirements

B201001 Exterior Enclosure As Indicated As Indicated Reinforced pre-cast concrete walls and roof. As indicated in Part A (Design-Bid-Build) Drawings included in Attachment A of Part 6

B201010 Exterior Joint Sealants As Designed As Required

B203006 Blast Resistant Doors 12 pairs As Indicated Swinging type blast resistant security doors as indicated in Part A (Design-Bid-Build) Drawings included in Attachment A of Part 6

B203008 Exterior Door Hardware

As Indicated As Indicated

Provide Internal Locking Device (ILD) system at each MSM as specified in B20 and indicated in Part A (Design-Bid-Build) Drawings included in Attachment A of Part 6.

B2040 Exterior Building Signage

As Designed As Designed Provide building numbers on exterior wall as per AAFB

ACBDS.

B2050 Waterproofing As Required As Required

Provide waterproofing system composed of waterproofing membrane, protection board, filter fabric, root barrier, sand cushion, drain tile and drain rocks along the building perimeter.

C3020 Interior Floor Finish As Required N/A Non-slip concrete clear sealer-hardener.

D304007 Exhaust Systems As Indicated As Indicated Passive exhaust ventilation system as indicated in Part A (Design-Bid-Build) Drawings included in Attachment A of Part 6

D50 Electrical Systems As Required As Required Provide electrical systems as specified in D50 and Part B (Design-Build) Drawings included in Attachment A of Part 6

CHAPTER 6: ENGINEERING SYSTEM

REQUIREMENTS

B20 EXTERIOR ENCLOSURE

FY22 MCAF PROJECT P-1366 / AJJY073105P2 APR - Munitions Storage Igloos, Phase 2 1181839

PART 3 - CHAPTER 6 / ESR B20 - Page 3

B202002 STOREFRONTS

Not used.

B202003 CURTAIN WALLS

Not used.

B202004 EXTERIOR GLAZING

Not used.

B202090 OTHER EXTERIOR WINDOWS

Not used.

B2030 EXTERIOR DOORS

Not used.

B203001 SOLID DOORS

Not used.

B203002 GLAZED DOORS

Not used.

B203004 OVERHEAD ROLL-UP AND OVERHEAD SECTIONAL DOORS

Not used.

B203006 BLAST RESISTANT DOORS

Provide special large double swing doors used for blast resistance at each MSM in accordance with the requirements indicated in the Part A Drawings included in Attachment A of Part 6 and Part A Specifications included in Attachment B of Part 6.

Hot dipped galvanizing of the blast resistant double door assemblies may not be practical. Provide zinc-rich anti-corrosive coating system suitable for the use of the system meeting minimum requirements provided in Part A Specifications included in Attachment B of Part 6.

Insulation of the blast doors is not required.

B203008 EXTERIOR DOOR HARDWARE

Provide each MSM door with Internal Locking Device (ILD) system in accordance with the Part A Drawings included in Attachment A of Part 6. The ILD is Government Furnished Government Installed (GFGI). The ILD bolt work and head and foot bolt is Contractor Furnished Contractor Installed (CFCI).

A dummy ILD will be available to the door installers for bolt work set-up and testing. Coordinate final ILD installation with the Contracting Officer.Provide single cylinder ILDs for the MSMs.

1181839 1571341

AMENDMENT No. 0005

PART 6: ATTACHMENTS

ATTACHMENT A: PART A DRAWINGS

S-002 – ABBREVIATIONS

S-702 – DOOR ELEVATIONS

S-703 – DOOR SECTIONS

S-705 – INTERNAL LOCKING DEVICES

A#DETAIL / SECTION TITLE

ABBREVIATIONS AND SYMBOLS

A4REFERENCE SYMBOL KEYA1REINFORCEMENT SPLICE SCHEDULE

B3CLOSED STIRRUP TIE DETAILB1STIRRUP AND TIE HOOK DIMENSIONS

C1STANDARD HOOK DIMENSION

S-002

09/13/2021

A1RIGHT DOOR ELEVATION - INTERIOR VIEW A3LEFT DOOR ELEVATION - INTERIOR VIEW

S-702

X

X Y

Y

X

X

Y

Y

D1SECTION D2SECTION D3SECTION D4SECTION

A1LEFT DOOR EXTERIOR PLATE AND INNER SUPPORT FRAMING A3LEFT DOOR INTERIOR PLATE AND HOLE LOCATION

C5SECTION X-X

B5SECTION X-X

S-703

B1INTERNAL LOCKING DEVICE (ILD) B4INTERNAL LOCKING DEVICE (ILD)

S-705

PART 6: ATTACHMENTS

ATTACHMENT I: NATURAL RESOURCES

PERFORMANCE WORK STATMENT

6/29/2021

e. Tag/label all specimens with long lasting forestry markers (aluminum tags) and assign a unique alpha numeric identifier.

f. Produce a qualitative cycad field assessment that will be used for the development of a Natural Resources Work Plan. The data collected from the field assessment will ascertain health conditions of the cycad material proposed/not proposed for salvage and include a descriptive proposal of which individual cycad basal shoots will be used for collection, or the rationale for not collecting cycad material. The health assessment must follow the criteria developed in the Conservation of Cycas micronesica on GuamMSA 1, Contract number N40192 17 R 8003 (Guam Plant Extinction Prevention Program 2019), found in Appendix B.

Tabernaemontana rotensis Approximately 15 individual T. rotensis have been identified within the project footprint of P 1366. The Contractor must conduct field assessments to identify the number of mature T.

rotensis within the project site (Figure 1) and evaluate mature plants. Mature plants will have reproductive structures or evidence of flower/ fruit. While in the field, the Contractor must collect all ripe fruit available. The goal is to collect seeds equal to the amount of mature plants lost by construction. The Contractor must search for flowers/ fruit on plants for eventual collection. The Contractor must:

a. Refer to the USFWS issued Biological Opinion for specific requirements associated with the salvage, collection, transplantation, and maintenance of T. rotensis.

b. Monitor mature plants to collect seeds up to the start of vegetation clearing.

c. Tag/label all mature T. rotensis plants and assign a unique alpha numeric identifier.

d. Record all mature T. rotensis locations with a Global positioning system (GPS) (see 3.8.f GIS

Standards) and submit to the KO.

e. Produce a qualitative T. rotensis field assessment that will be used for the development of a

Natural Resources Work Plan. The data collected from the field assessment will ascertain the material proposed/not proposed for collection, including the number of mature plants and seedlings.

3.5 WORK ELEMENT 5 Project Plans (Natural Resource Work Plans, Health and Safety Plans, HACCP Plan)

a. Salvage, Transplantation, Maintenance and Monitoring Natural Resource Work Plan (Natural Resource Work Plan). The Contractor must develop a Natural Resource Work Plan that includes details of the work prescribed in this PWS for natural resource mitigation for contractual compliance activities. The focus of the Natural Resource Work Plan must explain the details and protocols to collect T. rotensis seeds; germination of T. rotensis/cycad seeds (if collected); collection of cycad basal shoots; monitoring of plants in the nursery;

maintenance of outplanted plants in the field; and criteria for plants to be deemed established in the wild. The Government will provide examples of approved work plans of similar ESA listed plant salvage and transplantation projects. Successful salvage methods may be different from what is stated in the example work plan as successful methods are based on adaptive management. The Natural Resource Work Plan must include the following:

CYCAD MONITORING AND MANAGEMENT ON JOINT

REGION MARIANAS AREA OF RESPONSIBILITY –

Munitions Storage Area-1

September 2019

Cooperative Agreement N40192-17-R-8001

Cooperative Agreement Technical Representative

Catherine Brunson Natural Resource Specialist

36 Civil Engineering Squadron/Environmental (36 CES/CEV) Naval Support Activity Andersen

APPENDIX B:

CYCAD MONITORING AND MANAGEMENT ON JOINT

REGION MARIANAS AREA OF RESPONSIBILITY –

Munitions Storage Area 1

Prepared by:

James McConnell, Principle Investigator Rachel Jolley, Project Manager

Under Contract to:

36 Civil Engineering Squadron/Environmental (36 CES/CEV) Naval Support Activity Andersen

Agreement Dates: July 2019-September 2019

Recommended Citation:

Andersen Air Force Base. 2018. Health assessment and translocation feasibility of Cycas micronesica within the igloo footprint of Munitions Storage Area 1. Prepared under contract N40192-17-R-8001. September 2019.

Table of Contents Abstract

Introduction

Study Area

Methods

Results

Discussion

Conclusions

References

Appendices

Appendix C: Data

Appendix A: Photos and Charts

Appendix B: Decision Tree for Cycad Salvage

Electronic Appendices

Abstract The purpose of this cooperative agreement was to survey and assess all Cycas micronesica within the igloo footprint of Munitions Storage Area 1. This survey follows up on a previous survey of all federally listed plants within the area. Each cycad within the boundary was evaluated for the following criteria: alive or dead, condition, translocation potential, presence of pests (scale, leaf miner, stem borer, and butterfly), damage from ungulates, stem rot, sex, reproduction, the presence of pups, and habitat. This data was then summarized to indicate which trees might be used for seed collection, pup collection, or for translocation.

Introduction In preparation for the expansion of Munitions Storage Area 1 on Anderson Air Force Base, a survey was conducted of Cycas micronesica, a federally listed endangered plant, found within the footprint of the proposed construction area. The purpose of this survey was to determine the relative health of this population, possibility of translocation survivorship, available propagules, and to update the current population status. As part of this survey, a health index was developed to evaluate the condition of each tree. This index includes data on insect and ungulate pest damage, stem rot, reproduction data, and a general health assessment of C. micronesica tree found within the survey area.

Study Area This survey was conducted on Anderson Airforce Base (AAFB) in Northern Guam (Figure 1).

The survey area was the Munitions Storage Area 1 (MSA-1), specifically the area where there are plans to construct additional “igloos” for munitions storage (Figure 2). This area is referred to as the “MSA-1 igloo footprint” and is approximately 56 acres. The area is mostly remnants of limestone native forest, mixed with some small areas of savannah grasslands. Soil is mainly a mix of karst and clay and topography is quite flat.

Methods A survey was conducted on all the cycads falling within the boundaries of the MSA-1 footprint from July 29-August 1, 2019 in order to determine the health of each tree and the feasibility of translocating any trees. The survey was conducted by walking 10 m transects through the area. A previous survey of this area provided an estimate of where trees were located and how many trees fell within the boundary of the footprint. Trees were flagged with orange flagging and given a number. This number correlates to the data recorded in the GIS database. Each cycad within the boundary was evaluated for the following criteria:

alive or dead, condition, translocation potential, presence of pests (scale, leaf miner, stem borer, and butterfly), damage from ungulates, stem rot, sex, reproduction, the presence of pups, and habitat. Data was collected using a Trimble Geo 7x ®, so that the GPS coordinates for each tree could be linked to the database.

Data for most parameters consisted of indicating the presence/absence of criteria. There were some parameters that required an evaluation index. These included the following:

amount of stem rot, health status, and the translocation potential. Stem rot was measured using a 1-4 scale, where 1 represented less than 5% rot, 2 represented 5-10% rot, 3 represented 10-25% rot, and 4 was used when rot exceeded 25% of the stem (Figure 3). The tree’s condition was based on the number of leaves and the amount of stem rot. Since nearly every tree had some degree of insect pest damage, this was not used to determine condition. The prevalence of insect pests also made it unnecessary to create a category of “excellent”, as there were no trees that would have fit this description. The condition was designated as “good” if the tree had little to no rot and the presence of new leaves. The condition was designated as “fair” if the tree had no new growth but had an intact stem.

Trees designated at “poor” often had few to no leaves and a significant amount of stem rot (Figure 4). The translocation potential was based on the size of the tree (less than 10 ft was considered less difficult), the substrate (karst would make it difficult to remove the tree with the roots intact), the surrounding forest, and the presence of other tree roots intertwined with the cycad’s roots. Trees that were marked as ‘easy’ translocation potential, were generally less than 10 ft., had little to no karst in the soil, and were in open areas. Trees with ‘medium’ potential had one or two factors that might impose problems, and trees that were marked as ‘difficult’ had factors that would likely prevent the successful translocation of the tree (Figure 5).

In marking dead and alive trees, only dead trees that were still standing were surveyed (Figure 6). Trees that had fallen and were decomposing were not counted in the survey.

The presence of insect pest damage was also assessed in the survey (Figure 7). The presence of cycad aulacaspis scale (Aulacaspis yasumatsui) was determined if scale could be seen on the tree. The presence of the cycad leaf miner (Erechthias sp.) was determined if the leaves showed signs of necrosis. The presence of a stem borer was determined if there were holes and damage along the stem. Marler & Muniappan (2006) list three species of stem borers that cause damage to cycad stems: Dihammus mirianarum, Carpophilus mutilatus, and Dasyses rugosella. We were unable to identify the borers present in the stems, we only identified damage to the stem by a borer-type insect. The presence of the cycad blue butterfly (Chilades pandava) was determined if there were greater than 10% leaflets which had been destroyed on several cycad leaves. Ungulate damage was determined if the tree had been damaged around the base, with recent damage showing where rubbing had removed the outer layer of the stem, and past damage evident from scars and large holes around the base (Figure 8).

Cycads are dioecious species, so it is critical to know sex ratios in order to make decisions about a population. In C. micronesica, sex can only be determined if there are reproductive structures present. In this survey, reproductive structures were noted as microsporophylls (cones) for males, and either megasporophylls or mature seeds for females (Figure 9). Pups were counted by size class (0-5 cm, 5-10 cm, and greater than 10 cm). Pups that appeared dead (soft, dark brown leaf stipules) were not counted. Live pups either had green leaves or were firm with light brown/tan leaf stipules (Figure 10).

Results There was a total of 472 live trees within the footprint of the MSA-1 area (Figure 11).

There were 16 dead trees that were still standing. Most trees (364) were found in native limestone forest, with 109 found in open areas. Most trees were in a mix of karst and soil

(265), with 193 trees in soil only, and 14 in karst only (Figure 12). Of the live trees, there were 92 given the condition of “good”, 285 were considered “fair”, and 96 that were considered “poor” (Figure 13). There were 230 trees that had less than 5% stem rot, 115 trees had between 5-10% stem rot, 91 trees that had 10-25% stem rot, and 35 trees that had over 25% stem rot (Figure 13). The presence of ungulate damage and insect pests is also shown in Figure 13. There were 134 trees that were considered ‘easy’ to translocate, 198 were classified as ‘medium’, and 141 were classified as ‘difficult’ to translocate. It should be noted that the translocation potential was not linked to the health condition. Of the ‘healthy’ cycads, only 22 were categorized as ‘easy’ to translocate, 36 were considered ‘medium’ in translocation ease, and 34 were rated as ‘difficult’ to translocate (Figure 13).

There were 106 trees that could be determined as female, 48 that were determined to be male, and 319 that could not be determined. Of the female trees, 27 had seeds (though not all of these seeds were ripe/viable) (Figure 14). There were 191 trees with live pups, many trees with several pups. Total number of pups in each size class were: (0-5 cm) 378, (5-10

cm) 238, and (> 10 cm) 184 (Figure 15).

Discussion C. micronesica located within the footprint of the MSA-1 construction area were generally not in good health. All trees showed some level of insect infestation, resulting in damage to leaves and, in some cases, significant stem rot. More troubling is that there were no new seedlings observed anywhere within the footprint. However, these same issues are now common with nearly all cycads on Guam: mature trees are not in good health and there is no crop of new seedlings in the forest understory (Marler & Lawrence, 2012). On a positive note, one of the biological control agents for cycad scale (Aulacaspis yasumatsui), the lady beetle (Rhyzobius lophanthae), was observed on several trees in the northern section of the footprint (Moore, et al., 2005). It is hoped that as this insect becomes more established on Guam, it will help control the level of scale and improve the overall health of cycads.

Any effort to preserve this species should focus primarily on reproduction. As with any species, the greater the genetic diversity, the greater the probability that individuals with resistance to these threats will start to appear in the population. Currently trees are producing seed, but a significant portion of those seeds appeared aborted (small, shriveled and black). Trees with mature seeds (round, smooth, and greenish to tan in color) often had high levels of scale. In other nearby sites, such seeds will often germinate and produce seedlings. However, the mortality of these seedlings is nearly 100%, as they seem unable to withstand the scale which immediately overwhelms new seedlings. In other projects, mature seed is collected and brought back to the nursery where it is cleaned from scale and other pests and allowed to germinate ex situ. These seedlings can then develop free from scale and be returned to the native forests as juvenile trees.

Cycads reproduce asexually through the production of “pups”, vegetative outgrowths from the stem. These pups can potentially create new trees in the event the parent tree falls due to storm damage or rot. Recently, researchers have been investigating techniques of collecting pups from mature trees in an effort to propagate trees vegetatively ex situ. Pups are cut from trees aseptically and placed into a potting media with rooting hormone to stimulate root production.

The advantage of this method is that the collection of pups is much more economical, less labor-intensive, and likely less damaging than attempting to salvage and translocate an entire tree.

However, the propagation techniques for rooting pups are still being tested and has yet to be successful in a large-scale effort. While this method seems to work well with pups that have developed aerial roots, unrooted pups often succumb to infection from fungus on the cut surface (unpublished data).

In selecting pups to collect, it is recommended to collect from healthy trees. Pups from stressed trees have a reduced chance of survival, as they have depleted carbohydrate reserves. Stress can be caused by damage, drought, and extreme changes to the habitat (habitat clearing) prior to collection. Collected pups should be firm (not soft), with green leaves or tan stipules (if no leaves are present). Soft tissues and dark stipules generally indicate that the pup is not viable.

Often overlooked in this discussion is that cycads are dioecious. If an effort is made to salvage cycad genetics through the collection of pups, it is important to ensure that pups are collected in sufficient numbers from both male and female trees.

The translocation of mature cycads is another means of salvaging the unique genetic individuals from a forest facing destruction. This requires removing the entire tree and root system from its native forest and translocating the tree to a similar habitat. While this has been done successfully with Cycas micronesica on Guam (unpublished data), it requires a great deal of logistical support. Cycads, although relatively short trees, can weigh up to several hundred pounds. They also grow in limestone forests, with roots intertwined in both the karst soil and the roots of other trees. The removal of a tree from karst soil requires that roots are severed, making the probability of survival in the new habitat very unlikely. The best candidates for successful translocation are small trees growing in soil in open areas. However, in this survey, many of the trees in the more open areas were among the those in “fair” or “poor” health. Once again, in deciding which trees to salvage, it is important to collect both male and female trees.

To assist in deciding which method of salvaging is best for each tree, a decision-tree was created (Appendix B). This tool can be used by land managers to prioritize trees for seed collection, pup collection, or whole-tree salvaging.

Conclusions In salvaging cycads from a forest facing destruction, it is recommended that priority should be on collecting mature seeds for ex situ propagation. Seeds provide the best collection of genetic diversity from the local population, are easiest to collect, and have the highest probability of successful propagation, if they are treated for pests. Seeds should also provide a good mix of both male and female propagules.

As a secondary salvaging effort, pups can also be collected. The best success will likely come from healthy pups with aerial roots. Pups that are soft or brown are often decaying and should be avoided. Aseptic techniques will help prevent the spread of disease. Pups should be collected from both male and female trees in roughly equal proportions.

Whole-tree salvage is very labor intensive, expensive, and will likely be unsuccessful unless the tree is healthy and in a very accessible location. This method should be a “last resort” only if there is insufficient material available from seeds or pups. If trees are translocated, it is important to choose both male and female trees and replant them in close proximity to increase the chance of successful future reproduction.

References

Marler, T.E. and J.H. Lawrence. 2012. Demography of Cycas micronesica on Guam following introduction of the armoured scale Aulacaspis yasumatsui. J. Tropical Ecology 28:233–242.

Marler, T.E, and R. Muniappan. 2006. Pests of Cycas micronesica leaf, stem, and male reproductive tissues with notes on current threat status. Micronesica 39:1-9.

Moore, A., T. Marler, R.H. Miller, and R. Muniappan. 2005. Biological control of cycad aulacaspis scale on Guam. The Cycad Newsletter 28(5): 6-8.

Appendices

Appendix A: Photos and Charts

Appendix B: Decision Tree

Appendix C: Data

Appendix A: Photos and Charts

Figure 1. Map of Anderson Air Force Base (AAFB) in Northern Guam, highlighting the Munitions Storage Area 1 (MSA-1) in gold.

Figure 2. Proposed construction footprint for new munition storage igloos. Areas surveyed are outlined in the blue dotted line, with the exception of non-impacted areas.

Figure 3. Examples of cycads with varying degrees of stem rot. Starting in upper left corner, in a clockwise direction: a. level 1 (0-5% rot), b. level 2 (5-10% rot), c. level 3 (10-25% rot), d. level 4 (>25% rot).

Figure 4. Examples of cycads in varying health conditions: a. tree in “good” condition (although scale and leaf miner damage evident, new leaves and an intact stem indicate that this tree is fairly healthy); b. tree in “fair” condition (few new leaves, damage to stem); and c. tree in “poor” condition (no new leaves, significant stem rot).

Figure 5. Examples of cycads with varying translocation potential: a. cycad is “easy” to translocate due to its small size and the open area; b. cycad is “medium” in its translocation potential, due to larger size and karst soil, but still fairly accessible; c. cycad is “difficult” to translocate due to other tree blocking access and intertwined roots; d. cycad is also “difficult” to translocate due to large size, karst soil, other tree roots, and low accessibility.

Figure 6. Dead cycad still standing near living cycad.

Figure 7. Evidence of insect pests on cycads: a. damaged and missing leaflets indicate the presence of the cycad blue butterfly (Chilades pandava); b. the presence of leaf miners is indicated by necrosis of leaf tissue; c. stem borer damage indicated by damage to stem; d. cycad aulacaspis scale (Ausacaspis yasumatsui) is seen as white dots prevalent on new growth.

Figure 8. Evidence of ungulate damage on cycads: a. outer bark rubbed off and damage to inner tissues; b. hole at base is likely from past ungulate damage, with new tissue growing around to seal off dead tissue; c. former damage evident by scar.

Figure 9. Sex of cycads can only be determined by the presence of sporophylls: a. microsporophyll (cone) in early stage and (b) dehiscent stage of male trees; c. megasporophylls; and d. mature seed.

Figure 10. Examples of viable cycad pups: a. 0-5 cm, b. 5-10 cm, c. > 10 cm, d. pups longer than 30 cm were not considered.

Figure 11. Total C. micronesica trees located within the MSA-1 igloo footprint.

Figure 12. Community and soil types of cycads surveyed in the MSA-1 igloo footprint area.

Figure 13. Summary of health assessment, stem rot, pest damage, and translocation potential for cycads surveyed in the MSA-1 igloo footprint area.

Figure 14. Summary of reproduction and sex data for cycads surveyed in the MSA-1 igloo footprint area.

Figure 15. Summary of pup data for cycads surveyed in the MSA-1 igloo footprint area.

Appendix B: Decision Tree for Cycad Salvage

Tree alive?

no (don't salvage) yes male/Unkn own are pups present?

yes

Do pups have arial roots?

yes (collect) no

Is pup firm?

Tissues appear healthy?eeeeeeeeeeealaalaalalththhthhthhy?y???y?y?y?

yes

(collect) no (don't collect) no does tree appear vigorous?

yes is tree less than 10 ft tall?

yes are roots embedded in karst?

yes

(difficult to salvage) no (candidate for salvage) no (difficult to salvage) no (don't salvage) female are mature seeds present?

yes

Is scale present on seeds?

no/minimal collect seeds and treat scale yes do seeds appear aborted/mi sformed?

yes does tree appear vigorous?

yes is tree less than 10 ft tall?

yes are roots embedded in karst?

yes

(difficult to salvage) no (candidate for salvage) no (difficult to salvage) no (not a candidate for salvage) no

(collect seeds and treat scale) no are pups present?

yes

Do pups have aerial roots?

yes (collect) no

Is pup firm?

Tissues appear healthy?eeeeeeeeeeealaalaalalalththhthhththy?y???y?y?y?

yes

(collect) no (don't collect) no

Does tree appear vigorous?

yes is tree less than 10 ft tall?

yes are roots embedded in karst?

yes

(difficult to salvage) no (candidate for salvage) no (difficult to salvage) no (don't salvage)

Electronic Appendices

Excel data: MUNSCycas_micronesica

(GIS Data file)