13040 Plant Growth Chambers Growth Rms Tissue Culture R .pdf

USDA R&D FACILITY – ALBANY, CA JACOBS No. 25KT8810 TASK ORDER No. 53-3K15-1-0616 RECOVERY-MAJOR RENOVATIONS, PHASES 3-6

Jacobs ISSUED FOR FINAL SUBMITTAL February 18December 17, 2009 USDA, Recovery-Major Renovations, Phases 3-6 Page 1 of 35 Rev. 01 Section 13040, Plant Growth Chambers, Growth Rooms, Tissue Culture Rooms

SECTION 13040

PLANT GROWTH CHAMBERS, GROWTH ROOMS, AND

TISSUE CULTURE ROOMS

PART 1 - GENERAL

1.1 SUMMARY

A. The work under this section of the specification shall include all labor, services, materials, equipment, and the performance of all work as necessary to furnish and install all prefabricated Plant Growth Chambers and rooms as indicated on the drawings and as herein specified. The chambers and rooms shall be procured by the general contractor and installed under the supervision of a manufacturer’s representative. The general contractor shall produce approval plan and elevation shop drawings of all units to ensure space is available for installation, use, and maintenance prior to fabrication.

B. Plant Growth Chambers and rooms shall be furnished complete and operational and ready for installation with all accessories required for installation, whether included in this Section or not. In this section, “chamber” generally refers to a reach-in type unit and “room” refers to a walk-in type unit. This specification is not intended to supersede Government regulations or manufacturer’s standard construction if that construction is of equal or better quality to that specified and if acceptable to the Government.

C. The equipment locations, as shown on the plans, shall be checked by the Plant Growth

Chamber manufacturer. Exact locations shall be determined by the dimensions of the equipment approved and the layout prior to installation. The Plant Growth Chamber and room manufacturer shall be solely responsible for the accurate installation and correct operational aspects of his equipment and shall correct deficiencies without any recourse to the Government.

D. The Plant Growth Chambers and room manufacturer shall have a factory trained field service technician on the premises to supervise the installation of all equipment and assist the other trades as to the proper piping rough-in for equipment.

E. A factory-trained representative of the equipment manufacturer shall provide one onsite equipment demonstration to the Government.

F. The Plant Growth Chambers and room manufacturer shall be responsible for coordination of all utility connections

G. Delivery and installation of the equipment shall be so performed as to avoid delay to the work of other contractors. The Plant Growth Chamber and room contractor shall be responsible for delivery, receiving, uncrating, and installing this equipment. All internal wiring relevant to the units operation, including light fixtures, are to be supplied and installed by the Plant Growth Chambers and room manufacturer and terminated at a single point for final connection by relevant trade contractor.

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H. The Plant Growth Chamber and room contractor shall coordinate their work with the General Contractor, Construction Manger and all supporting contractors, providing all information and supervision required to assure proper and timely installation without delay to the project or other contractors.

1.2 RELATED WORK

A. Division 01 Specifications

B. Section 03300, Cast-in-Place Concrete: Floor slabs.

C. Section 07920, Joint Sealants

D. Section 09250, Gypsum Board

E. Section 09510, Acoustical Ceilings

F. Section 13930, Wet Pipe Fire Suppression Sprinklers

G. Section 15810, Ductwork and Ductwork Accessories

H. Section 15400, Plumbing Systems

I. Section 16050, Basic Electrical Materials and Methods

J. Section 16081, Apparatus Inspection and Testing

K. Section 16082, Follow-Up Testing for Electrical Equipment

L. Section 16402, Interior Distribution System

M. Data sheets and Drawings for each chamber and room.

1.3 STANDARDS

A. Americans with Disabilities Act (ADA).

B. National Fire Protection Association (NFPA)

1. 220 Standard on Types of Building Construction

2. 255 Standard Method of Test of Surface Burning Characteristics of Building

Materials.

3. 70 Electrical Components, Devices and Accessories.

4. 79 Electrical Standard for Industrial Machinery.

C. Operating equipment shall conform to Underwriters Laboratories (UL).

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D. Panel construction shall comply with Factory Mutual (FM) 4880

E. The Plant Growth Chambers and room design, manufacture, and installation shall conform to applicable codes, ordinances and regulations governing the use and safety of refrigerants, including, but not necessarily limited to the following:

1. ASHRAE/ANSI Standard 15-70, ARI 520-78, ANSIB9.1-1971 and NEMA-70.

2. National Sanitation foundation Testing Laboratory, and Class One Building Type

Construction of FM approved standard #4411 for insulated wall construction.

3. Approval by Underwriter’s Laboratory (UL), labeled and listed, for all components of the Chamber/ assembly.

4. Local code approval for panels.

5. Local health, sanitary and safety codes.

6. Local seismic construction codes.

F. NRTL: Chambers and rooms shall be certified, as an entire unit, to a registered NRTL (Nationally Recognized Testing Laboratory) standard.

1.4 QUALIFICATIONS

A. Submit a statement of qualification showing adequate experience in manufacturing equipment of the type and scope called for with a minimum of five years experience.

The statement of qualification shall include a list of a least five comparable recent installations for each type of equipment proposed. This list shall have current contact details including names, telephone numbers and email addresses.

B. Service and Maintenance: Vendor shall be a manufacturer of Plant Growth Chambers and rooms which maintains factory parts and service. An extended warranty service contract shall be tendered at least one month prior to the end of the warranty periods for consideration by the Government.

C.Vendor shall be an ISO 9001 registered and compliant company.

1.5 SUBMITTALS

A. Submit the following in accordance with Section 01330, Submittal Procedures.

1. SD-02 Shop Drawings

• Submit manufacturers data sheets and approval drawings for Plant Growth

Chambers and rooms showing service clearances; details and location of anchorage and fitting to floors; details of features, control panel design;

wiring diagram; sensor location; dimensions and weights; layout of units with relationship to surrounding walls, doors, lighting and ventilation fixtures, and other building components.

• Manufacturer’s data sheets shall include the performance capabilities for each chamber. Include refrigeration system efficiency data, refrigerant type, USDA, Recovery-Major Renovations, Phases 3-6 Page 4 of 35 Rev. 01 refrigeration system schematic, sequence of operation

• Include all service connection information

• Exterior and interior finish.

• Adjustable shelf sizes, locations and attachment method

• Electrical outlet locations, interior and exterior

• Lighting equipment data

• Plant watering system data, where applicable

2. SD-07 Certificates:

• Qualification Data: Prior to executing a contract for the work of this Section, submit qualification data for proposed manufacturer as specified under “Qualification” to demonstrate its capabilities and experience. Include list of completed projects, and other information specified. Include manufacturer’s technical data and specifications in sufficient detail for Architect and Government to assess quality and suitability of products proposed.

• Certificates: Submit certified statement by issuing agencies that manufacturer has been issued the following certificates:

• CFC Compliance: Insulated panels shall comply with current EPA Regulations and the Clean Air Act. Manufacturer shall supply Notarized Affidavit that the insulated panels are a Class I foam type that conforms to current statutes in effect at date of job site arrival. CFC emission compliance foam type must be Factory Mutual Standard 4880, including full scale corner test; UL E-84. The affidavit shall hold harmless and indemnify the Government, the Architect/Engineer, and the equipment consultant from any fines, summons or liabilities which may result from a violation.

• EPA Venting Issue: The manufacturer shall submit a certificate that one (1) job site mechanic has received accredited training conforming to the EPA and Clean-Air Act. Any sub-manufacturer and/or general refrigeration manufacturer shall assume full responsibility for the actions of the employees or casual helpers in respect to “venting” of EPA regulated refrigerant gases.

Furthermore, manufacturer will supply an affidavit which specifically indemnifies and holds harmless the Government, the Architect/Engineer, and the equipment consultant from any fines, summonses or liabilities which may result from “Venting Controlled Refrigerants”.

4. SD-10 Operation & Maintenance Data:

• Instructions for sequential operation, start-up and shut down, with pertinent control data and schematics, chamber arrangement, and recommended maintenance of equipment.

• At completion of work, submit one copy of 3-ring binder containing: “as-built” drawings, product data and operation and maintenance information listed above.

• After Architect’s acceptance is received, submit 4 copies for Government’s use.

B. Substitutions:

1. All exceptions shall be subject to written approval prior to receipt of bid. If no written communication is received prior to receipt of bid and approval indicated

USDA, Recovery-Major Renovations, Phases 3-6 Page 5 of 35 Rev. 01 in an addendum, it is assumed that bidder will be in total compliance with specifications and will be held responsible for default or delay, regardless of any statement to the contrary in their written proposal.

2. Requests for a substitution must be made directly to the Government’s

Representative’s office for consideration no later than 15 working days prior to bid receipt date.

3. Requests for a substitution following the bid opening will be rejected.

4. Substitutions approved prior to bid date will be handled as an addendum and be sent to all bidders.

C. No portion of the work requiring a submission shall be commenced until the submission has been approved by the Government’s Representative. All such portions of the work shall be in accordance with approved shop drawings and samples.

1.6 FIELD MEASUREMENTS

A. It is the Plant Growth Chamber Rooms and chamber manufacturer’s responsibility to verify field measurements and that equipment will fit through entryways, corridors and door openings enabling a smooth flow of equipment to its proper location in the building.

1.7 WARRANTY

A. Furnish a written warranty that Work performed under this Section to be and remain free from defects as to materials and workmanship for a period of two years from date of acceptance. Defects in materials and workmanship that may develop within this time are to be replaced without cost or expense to the Government. Defects include, but are not limited to operation or control system failures, performance below required standards, excessive wear, unusual deterioration or aging of materials of finishes, the need for excessive maintenance, and similar unusual, unexpected and unsatisfactory conditions.

Prominently display in each room the name of install/service agency to be contracted during warranty period.

PART 2 - PRODUCTS

2.1 ACCEPTABLE MANUFACTURERS

A. Manufacturers deemed to be capable of meeting the requirements of this Section are as follows:

1. Conviron, Controlled Environments, Ltd. (Winnipeg, Manitoba, Canada)

2. Environmental Growth Chambers (Chagrin Falls, Ohio)

3. Biochambers, Inc. (Winnipeg, Manitoba, Canada)

3. Percival Scientific, Inc. (Perry, Iowa)

2.2 CONTROL SYSTEM

A. Control system shall apply to all chambers and rooms within this Section.

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B. General: Robust industrial grade control system shall be designed specifically for plant growth chambers and rooms. Include color touch screen interface with scalability.

C. Programming: Provide control system capable of storing up to 16 real time user-entered programs, each containing up to 48 lines, with one-minute resolution.

D. Data Logging and Graphing: Ensure inputs and controlled parameters are automatically logged to provide a record of experimental parameters. Provide local screen where trend graph is used to display main chamber processes (up to 5 days). Ensure pan and zoom functions are available to increase resolution. All chamber inputs and outputs shall be logged to daily text files that are easily retrievable from any Ethernet connected computer. Provide service diagnostics.

E. Alarms: Provide both audible and color-coded visual alarms. Provide user-settable absolute alarms for all controlled processes and user-settable process tracking alarms for temperature. Alarm history shall be logged to daily text files, easily retrievable from any Ethernet-connected computer or through the local control screen (minimum last 15 alarm events).

F. Security: Password protected levels shall be reserved for users (minimum three), administrator (minimum one), technicians, and factory service personnel.

G. Startup Delay: Chambers shall be programmable with varying restart times to reduce startup loads after a power failure.

H. Communications: Provide Ethernet connectivity. Provide independently settable controller, mask and host IP address.

I. Auxiliary contacts: Provide alarm contacts for connection to the Building Automation

System (BAS).

2.3 CONSTRUCTION

A. Dimensions: Dimensions for proposed equipment shall be in accordance with the individual equipment data sheets.

B. Cabinet Construction (Chambers): Provide woodless CFC-free polystyrene insulation.

C. Wall Construction (Rooms): Wall panels shall be of sheet metal construction with 4” of foamed-in-place CFC-free polyurethane insulation. All panels shall be assembled from the room interior with camlock or similar mechanical fasteners.

D. Exterior and Interior Finish - Chambers and Rooms: Provide white enamel baked-on patterned aluminum (exterior) and reflective white enamel baked-on smooth aluminum (interior). Provide specular aluminum finish on interior end walls of chambers when indicated on equipment data sheets.

E. Drain Pan (Chambers): Provide 22 gauge minimum stainless steel drain pan under entire growth area.

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F. Doors

1. Chambers: Provide reach-in doors with keyed magnetic locks, each with clear openings as indicated on the individual equipment data sheets.

2. Rooms: Provide walk-in door with clear opening minimum of 34” x 76”. Each door panel shall incorporate min 12 ga. vertical stainless steel channels (foamed-in-place) on each side of door opening. Door shall be self-closing with key locked door latch equipped with interior safety release and shall incorporate magnetic snap-in perimeter gasket. Doors for rooms operating below 5ºC shall have an anti-sweat heater installed. Door heater shall be factory pre-wired to control cabinet. Door shall incorporate a 3-pane observation window, minimum 14” x 14”, with a light-tight cover.

G. Control Panel: Provide left-hand or right-hand configured control panel in accordance with the individual equipment data sheets.

H. Receptacles:

1. Chambers: Provide convenience receptacles (20 amp duplex) in accordance with the individual equipment data sheets.

2. Rooms: Provide electrical receptacles (20 amp duplex) in accordance with the individual equipment data sheets. Receptacles shall be flush-mounted vapor proof fixtures foamed-in-place with the wall panel. Receptacles shall be mounted at 48” above floor. Use EMT conduit for connections to each receptacle and all conduit shall be hidden within wall panels.

I. Floor:

1. Chambers: Chamber floor shall have a minimum load capacity of 60 psf and be constructed of aluminum.

2. Individual rooms requiring a floor are stipulated on the data sheets. Where a floor has been stipulated, load capacity shall be a minimum 100 psf and withstand point loads of 300 pounds. Floor shall be 4” thick and insulated and shall incorporate an internal ramp not exceeding a slope ratio of 1:12. Floor shall be slip-resistant aluminum diamond tread plate or approved equal.

J. Air Handling Plenums (Chambers): The equipment data sheets shall stipulate airflow direction.

1. Where horizontal airflow is specified, manufacturer shall incorporate a pressurized air handling plenum located on the rear wall of the chamber. Plenum shall incorporate perforations to ensure uniformity of airflow across each shelf within chamber.

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2. Where vertical airflow is specified, manufacturer shall incorporate perforations on vertical faces of floor channels in combination with a pressurized underflow plenum to ensure uniformity of airflow within chamber.

K. Closure Strips (Rooms): Closure strips of materials matching wall panels for each room shall be provided and installed between the roof line of the rooms and other facility walls, columns, suspended ceilings, or ledges.

L. Suspended Ceiling (Rooms): Provide diffusion grating of injection molded translucent white plastic consisting of multiple open cells installed below the room ceiling panels.

All room lighting and evaporator coils and heat assemblies shall be installed within this space.

2.4 LIGHTING

A. Lamps and ballasts (Chambers): Lighting systems shall incorporate a combination of T5 or T8 fluorescent and tungsten incandescent lamps in a proportion of 80:20, respectively, by lamp wattage. Where variable light intensity has been stipulated on the data sheets, manufacturer shall incorporate electronic dimming ballasts combined with quantum light sensor to enable closed-loop intensity control, programmable by the micromole at the chamber’s control panel. Where the data sheets indicate stepped lighting, control manufacturer shall provide a minimum of three-step control of each lamp type.

B. Light Intensity (Chambers): Light intensity values are expressed in micromoles per meter squared per second. Manufacturer shall ensure minimum values are achieved as measured at a distance of 150mm from the lamps at 25º C.

C. Ballast Cooling (Chambers): Manufacturer shall ensure ballasts are actively cooled using circulating fans or equivalent.

D. Lamp Fixture (Chambers): Where horizontal airflow has been stipulated, manufacturer shall incorporate a non-yellowing acrylic barrier between the lamps and growth area.

Barrier shall be hinged for access to lamps from inside of the chamber.

E. Lamp Fixture (Rooms): Provide visibility lighting using T5 or T8 fluorescent lamps to maintain an average light intensity of 100 footcandles at 36” above the room floor at room operating temperature. Light fixtures shall be enclosed in a vapor-proof gasketed fixture and mounted above the room suspended diffusion grated ceiling and shall be controlled by an external switch mounted at 48” elevation adjacent to the door latch.

2.5 TEMPERATURE CONTROL AND ALARMING:

A. Performance Range: Temperature performance ranges shall be in accordance with the equipment Data Sheets. Temperature control shall be +/- 0.5oC at the control point.

Temperature uniformity within the walk-in rooms shall be +/- 1.0oC as measured at 300mm from all walls and ceiling, and as measured from 900mm from the floor.

Temperature safety limits shall be incorporated into all Chambers and Rooms as follows:

1. Primary: Provide programmable high and low temperature limit tracking alarm that automatically follows the programmed set point.

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2. Secondary: Provide an independent, high and low temperature limit.

3. Ensure both audible and visual alarms are provided from Primary and Secondary alarms and ensure that activation of safety limit set point turns power off to the chamber or room.

B. Personnel Emergency Alarm (Rooms): Each room shall be provided with reset type, electrically powered personnel emergency alarm system. Alarm shall consist of a actuator within the room and audible and visual alarms affixed to the front exterior of the room. The alarm system actuator shall be heavy duty type, oil-tight switch, equipped with a red button marked "EMERGENCY ALARM - PULL TO RESET". Actuator shall be mounted on the interior wall of the room adjacent to the doorjamb.

2.6 AIR EXCHANGE:

A. Conditioning (Chambers): Ensure that external air to chamber is conditioned prior to entering the chamber area.

B. Exchange Rate (Chambers and Rooms): Provide minimum fresh air exchange rates as stipulated on the Data Sheets. Fresh air shall be sealable and adjustable up to maximum rates stipulated.

2.7 ADDITIVE HUMIDITY CONTROL:

A. Application: Requirement for additive humidity control is stipulated by chamber type on the Data Sheets.

B. Methodology: Where additive humidity is stipulated on the data sheets, manufacturer shall incorporate atomizing spray nozzle methodology. Nozzles shall be placed within air-handling plenum of chamber. System shall incorporate a dry humidity sensor for feedback control.

C. Performance Range: Refer to data sheets.

2.9 CONDENSATE PUMP (applies to some units, not all)

A. Pump shall be Little Giant model VCC-20ULS provided and installed by the growth chamber/room manufcturer. Provide circuit breaker integral to plant growth chamber for the following units: 1-14B, 1-14C, 2-17, 3-14A, 3-14B

B. The general contractor shall provide and install a condensate pump for each of the other chambers/rooms. Refer to electrical and plumbing drawings.

2.10 IRRIGATION SYSTEM:

A. Application: Requirement for programmable ebb and flow irrigation system is stipulated by chamber type on the data sheets.

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B. Methodology: Where automatic irrigation is stipulated on the Data Sheets, manufacturer shall incorporate tray type ebb and flow irrigation. Tray shall use sump methodology with adjustable height to facilitate varying flood depth capability. Automatic fill nozzles shall be provided, and flood start time shall be programmable through controller.

C. Performance Range: Ebb and flow irrigation system shall be adjustable to a depth of 2".

2.11 PRE-ASSEMBLED REFRIGERATION SYSTEM

A. Mounting Location and Access: Chamber and room refrigeration system mounting locations shall be in accordance with the equipment data sheets. On-board refrigeration systems shall be mounted within the chamber confines. Manufacturer shall ensure access is provided readily to all major mechanical components of the refrigeration system from the exterior of the chamber. Remotely mounted refrigeration systems shall ensure that a single skid containing the water-cooled condenser, compressor, receiver, suction accumulator, suction line filter, controls, sight glass, and pressure regulating valves is provided at the remote location. Refrigeration system shall be designed and sized to accommodate the chamber and room operating conditions. Remotely mounted refrigeration systems shall be designed for placement up to 50 total pipe feet from the chamber or room location, and for a maximum vertical rise or drop of 25’ over the 50’ distance. All water-cooled condensers shall have a constant flow of cooling water.

B. Refrigerant: The refrigeration systems shall use refrigerants that are non-ozone depleting including R134a, R-404A, or approved equivalent.

C. System Integrity: Refrigeration system shall be provided with replaceable suction line filters and maintenance isolation valves. System design shall ensure to enable the compressor to operate continuously with a modulating bypass system to maintain specified temperature ranges.

D. Refrigeration Methodology: Refrigeration system shall be water-cooled and consists of a hermetically sealed condensing unit with hot gas bypass. All chambers and rooms shall integrate a modulating valve to proportion and regulate heating and cooling of the chambers. Individual heating and cooling circuits using solenoid valves shall not be used.

E. Components: Heat exchanger shall be copper-tubed construction with aluminum fins.

F. Defrost System (Rooms): Any room operating below 4oC shall incorporate an automatic defrost. Defrost system shall incorporate heaters to prevent condensate pan or drain line freezing. Automatic bypass of the defrost cycle shall be provided for use when room is operated above 4oC. Automatic defrost system shall be factory pre-wired to the control cabinet with all necessary control panel programming.

G. Evaporator (Rooms): Evaporator shall be low-profile, forced air design and complete in all respects including cabinet, coils, fans, wire fan guards, piping, expansion valve, sloped drain pan with heaters, hanger bars and attachment points, electric or hot gas defrost and ancillary components necessary to achieve specified performance criteria.

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H. Refrigeration Piping (Chambers and Rooms): All refrigeration piping for both on-board and remotely mounted refrigeration systems shall be provided by the controlled environment manufacturer. Provide ACR type, hard drawn, cleaned and capped Type L copper tubing. All piping shall be pressure (vacuum) leak 24 hour tested and witnessed by the Government. Provide P-traps on the vertical rise of refrigerant lines as required.

Insulate refrigerant piping with closed cell flexible foam plastic (Armaflex) or equivalent.

2.12 NEW GROWTH CHAMBERS AND ROOMS

A. List of new growth rooms and reference Drawings. Refer to data sheets for each growth room/chamber for detailed requirements. Verify with the Government the number and location of new units required for each project phase.

LEVEL EQUIP # EQUIPMENT

TYPE

TYPE REFERENCE DWGS

Basement B-1 Growth Chamber Reach-in M202-R, Q300-R Basement B-2 Growth Chamber Reach-in M202-R, Q300-R Basement B-3 Growth Chamber Reach-in M202-R, Q300-R Basement B-4 Growth Chamber Reach-in M202-R, Q300-R Basement B-5 Growth Room 3-Room Walk-in M202-R, Q300-R, Sketch

B-5-1 and B-5-1A Basement B-6A Growth Chamber Reach-in M202-R, Q300-R Basement B-6b Growth Chamber Reach-in M202-R, Q300-R Basement B-7 Growth Chamber Reach-in M202-R, Q300-R Basement B-8 Growth Chamber Reach-in M202-R, Q300-R Basement B-9 Growth Chamber Reach-in M202-R, Q300-R Basement B-11 Growth Chamber Reach-in M202-R, Q300-R Basement B-12 Growth Chamber Reach-in M202-R, Q300-R Basement 2-15 Growth Chamber Reach-in M202-R, Q300-R First Floor 1-14B Growth Chamber Reach-in M211-6, Q301-6, Q303-5b First Floor 1-14C Growth Chamber Reach-in M211-R, Q301-6 Second Floor 2-17 Growth Room 1-Room Walk-in M221-6, Q302-6, Sketch 2-17-1 and photo Third Floor 3-14A Growth Chamber Reach-in M231-6, Q303-6, Q303-5b Third Floor 3-14B Growth Chamber Reach-in M231-6, Q303-6, Q303-5b

2.13 EQUIPMENT DATA PLATE

A. An aluminum equipment data plate shall be permanently attached to the front of each chamber or room. The following data shall be stamped or engraved into the plate:

Manufacturer’s address and phone:

Data of manufacture:

Model Number:

Purchase Order Number:

V/Ph/Hz/Wires:

Full Load Amps:

Max BTU/hr heat rejected to cooling water:

Max BTU/hr heat rejected to 75º ambient air:

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PART 3 - EXECUTION

3.1 GENERAL

A. All units must be assembled in the factory and tested to demonstrate compliance with temperature, RH and lighting specifications and tolerances. The results of the test will be sent to the Government’s Representative for his review and approval. Only after this approval is given may the manufacturer disassemble the unit and ship the unit to the job site for installation. The Government’s Representative reserves the right to be present when the test is done. All costs associated with this test, including but not limited to the inspection team’s travel expenses (assume one person), are part of this section of the specification. All other units to be field tested. Provide a separate line item in submittal for these expenses.

3.2 EXAMINATION

A. Examine and verify areas and work of other trades for the following:

1. Correct dimensions.

2. Properly located utilities.

B. Report any unsatisfactory field condition to the Contractor in writing. Do not proceed with installations until unsatisfactory conditions have been corrected and conform to project requirements.

3.3 PREPARATION

A. Scheduling and Coordination: The chamber installer shall examine project conditions at the site with regard to access, dimensions, conditions as they exist and the general areas of work, and shall perform work in such a manner as required to deliver, install and connect the chamber in close coordination with work of other trades.

3.4 INSTALLATION

A. Install Plant Growth Chambers in accordance with manufacturer’s recommendations and final approved shop drawings.

1. Install components straight, plumb, level and true. Install service lines at right angles to walls and floors, except where required to pitch to drains.

2. Seal or otherwise insure that fastenings to rooms do not compromise vapor barriers or insulation. Seal between all piping and sleeves.

B. Pressurize and leak test entire system at not less than 100 psig, clean and dehydrate by maintaining a vacuum of 50 microns, or lower, for a 5 hour period. Add required charge or refrigerant, and oil if necessary, and test entire system for performance. Mark each system clearly as to refrigerant type and quantity used.

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C. Provide filler panels to enclose spaces between tops of rooms and ceiling of surrounding area. Extend filler panels to underside of construction above if necessary.

D. Install shelving assemblies in accordance with manufacturer’s instructions.

E. Install condensate pump and route discharge pipe to drain header.

3.5 PERFORMANCE TESTING

A. General: Provide equipment for the performance testing. Tests shall confirm that the Plant Growth Chambers conform to the following requirements:

1. Temperature control of +/-0.1°C shall be the temperature at the sensor and shall be the total variation in the temperature control of the room. It should not be confused with temperature uniformity as noted below.

2. Temperature uniformity of +/-1.0°C refers to the maximum allowable variation in temperature as measured on a horizontal plane 40” above finished floor. and within 12” of walls throughout the entire chamber. Room shall recover preset operating temperature within 5 minutes after door has been fully opened to 75°F ambient temperature for a period of 1 full minute. Repeat door opening operation, as described, 3 times during the 24 hour period, at least 1 hour apart.

3. After completion of installation each chamber shall be tested for a minimum of

24 hours at extremes of temperature specified on data sheets. Rooms specified to operate over a range of temperatures shall be tested for 24 hours at each of two set points (to be determined) with the second test at the set point designated for operation at acceptance of the unit.

B. Government’s representatives shall be given the option of witnessing and confirming test results. Notify Government’s representative in writing, prior to test.

3.6 CLEANING AND PROTECTION

A. Repair or remove and replace defective work, equipment and accessories as directed upon completion of installation.

B. Clean exposed and semi-exposed surfaces, touch-up finish as required. Remove and refinish damaged or soiled areas.

C. Protection: Adequately protect the work from damage until final acceptance by the

Government.

END OF SECTION 13040

DATA SHEET – GROWTH CHAMBERS

Client: U.S. Dept of Agriculture Project No.: 25KT8810 Equip. No.: B-1 (pg 1 of 2) Service: P&ID No.: N/A Data Sheet No.:

Manufacturer: Conviron or equal Sys. Spec. No.: 13040 Plant Loc.: Albany, California Basis of design Model #: PGR14 Quantity: 1 Equip. Loc.: RERA basement Equipment Name: REACH-IN GROWTH CHAMBER

Performance Criteria:

Ambient building conditions: 60º F to 85º F, no RH control Minimum chamber temp required Lights on:10º C Lights off: 4º C Maximum chamber temp required Lights on:45º C Lights off: 45º C Uniformity of temperature required (defined on horizontal plane thru conditioned area.): +/- 0.5 deg C Minimum relative humidity required Maximum relative humidity required Lights Off: 90% Lights On:85% Relative Humidity tolerance: +/- 3% Filtration for insects? Yes / No Mesh size required:

If yes – Kind of insect(s): Size of insect(s):

Filtration for dust: Yes/No If yes, particle size removed:

Carbon dioxide control? No If yes, range:

Maximum light intensity required 875 uMol/sq m/s What distance from lamps:

Source of light used Fluorescent tubes with dimming ballasts and incandescent lamps Barrier or open lamp canopy:

Levels of light control required Dimmable to 100 uMol/sq m/s Preference for airflow direction Yes If yes, advise: vertical upflow Automatic watering required No If yes, describe:

Size Consideration:

Reach-In or Walk-In design: Reach in Max size: 95”W x 35”D x 84” H Growth area: 14 square feet Growth height: 46 inches Shelving in room: 1 shelf Size of shelves: 1 at ___” x ___”

1 at __” x __”

Install location vertical space: 10’-7” (field-verify) Building Services Required:

Est. electrical service required: 208volt, 3 phase, 60 hz, 30 amps

4 wire plus ground Single point of connection

Cooling water: gpm: 5.2 Entering water temp: 85º F Heat rejected to cooling water: BTU/hr: 26,000 Leaving water temp: 95º F Demineralized humidification water: Maximum Flow: 1.1 US gal/hr Quality required: pH 7.0 +/- 0.5

Resistivity: 0.5 to 1.0 Meg Ohms Filtration: less than 2 microns

Compressed air: None Pressure: Dewpoint: SCFM:

Drains Connection: 1” diameter Building alarm system REES Certifications required:

CSA/NRTL or UL for the chamber assembly, not just for components

Potable water for plant watering: No

EQUIPMENT NO. B-1 (Pg 2 of 2) Refrigeration:

Refrigerant Type: R-134a, R-404A or approved equal Refrigerant Charge (lbs):

Number of circuits: * Number of compressors * Compressor type: * Number of cooling stages: * Number of heating stages: * Capacity control type: * Refrigerant metering device: * Growth Chamber Indoor Coil:

Indoor coil material: * Indoor coil number of rows/fins: * Growth chamber indoor coil airflow: * Blower motor HP: * Blower size and rpm: * Cooling capacity (btu/hr): * Heating capacity (btu/hr): * Water Cooled Heat Exchanger: Mounted on chamber B-1 Water flow (gpm): * Variable or constant flow: Constant Entering water temp maximium (ºF): 85 Entering water temp minimum (ºF): 45 Water pressure drop (feet of water): * Control System:

User interface: Color touch screen with scalability Alarm: Audible and color coded visual alarms, automatic shutoff at high temperature

User settable alarm points for all controlled processes.

Internet connectivity: yes Programmability: Up to 16 real-time user entered programs each containing up to 48 lines with one minute resolution

Data Logging and graphing: Inputs and controlled parameters automatically logged. Local screen trend graph displays up to 5 days of main chamber processes

Chamber inputs and outputs logged to daily text files easily retrievable from Ethernet connection.

Service diagnostic capability: yes

Security: Password protection for administrator and three users Start-up delay: Programmable variable start up times after power failure to reduce start-up loads

REMARKS:

Prepared by: Rev. No. Rev. by Date Approved Date:

Checked by:

Approved:

Client: USDA

* Information to be supplied by manufacturer.

Client: U.S. Dept of Agriculture Project No.: 25KT8810 Equip. No.: B-2 (pg 1 of 2) Service: P&ID No.: N/A Data Sheet No.:

Manufacturer: Conviron or equal Sys. Spec. No.: 13040 Plant Loc.: Albany, California Basis of design Model #: PGR14 Quantity: 1 Equip. Loc.: RERA basement Equipment Name: REACH-IN GROWTH CHAMBER

Performance Criteria:

Ambient building conditions: 60º F to 85º F, no RH control Minimum chamber temp required Lights on:10º C Lights off: 4º C Maximum chamber temp required Lights on:45º C Lights off: 45º C Uniformity of temperature required (defined on horizontal plane thru conditioned area.): +/- 0.5 deg C Minimum relative humidity required Maximum relative humidity required Lights Off: 90% Lights On:85% Relative Humidity tolerance: +/- 3% Filtration for insects? Yes / No Mesh size required:

If yes – Kind of insect(s): Size of insect(s):

Filtration for dust: Yes/No If yes, particle size removed:

Carbon dioxide control? No If yes, range:

Maximum light intensity required 875 uMol/sq m/s What distance from lamps:

Source of light used Fluorescent tubes with dimming ballasts and incandescent lamps Barrier or open lamp canopy:

Levels of light control required Dimmable to 100 uMol/sq m/s Preference for airflow direction Yes If yes, advise: vertical upflow Automatic watering required No If yes, describe:

Size Consideration:

Reach-In or Walk-In design: Reach in Max size: 95”W x 35”D x 84” H Growth area: 14 square feet Growth height: 46 inches Plants: corn, tomato, tobacco

Est. electrical service required: 208volt, 3 phase, 60 hz, 30 amps

4 wire plus ground Single point of connection

Cooling water: gpm: 5.2 Entering water temp: 85º F Heat rejected to cooling water: BTU/hr: 26,000 Leaving water temp: 95º F Demineralized humidification water: Maximum Flow: 1.1 US gal/hr Quality required: Ph 7.0 +/- 0.5

Resistivity: 0.5 to 1.0 Meg Ohms Filtration: less than 2 microns

Compressed air: None Pressure: Dewpoint: SCFM:

Drains Connection: 1” diameter

EQUIPMENT NO. B-2 (pg 2 of 2)

Refrigerant Type: R-134a, R-404A or approved equal Refrigerant Charge (lbs):

Number of circuits: * Number of compressors * Compressor type: * Number of cooling stages: * Number of heating stages: * Capacity control type: * Refrigerant metering device: * Growth Chamber Indoor Coil:

Indoor coil material: * Indoor coil number of rows/fins: * Growth chamber indoor coil airflow: * Blower motor HP: * Blower size and rpm: * Cooling capacity (btu/hr): * Heating capacity (btu/hr): * Water Cooled Heat Exchanger: Mounted on chamber B-2 Water flow (gpm): * Variable or constant flow: Constant Entering water temp maximum (ºF): 85 Entering water temp minimum (ºF): 45 Water pressure drop (feet of water): * Control System:

User interface: Color touch screen with scalability Alarm: Audible and color coded visual alarms, automatic shutoff at high temperature

User settable alarm points for all controlled processes.

Internet connectivity: yes Programmability: Up to 16 real-time user entered trend graph displays up to 5 days of main chamber processes

Chamber inputs and outputs logged to daily text files easily retrievable from Ethernet connection.

Service diagnostic capability: yes

Security: Password protection for administrator and three users Start-up delay: Programmable variable start up times after power failure to reduce start-up

Checked by:

Approved:

DATA SHEET – GROWTH CHAMBER

Client: U.S. Dept of Agriculture Project No.: 25KT8810 Equip. No.: B-4 (pg 1 of 2)

Service: P&ID No.: N/A Data Sheet No.:

Manufacturer: Conviron or equal Sys. Spec. No.: 13040 Plant Loc.: Albany, California Basis of design Model #: ATC26 Quantity: 1 Equip. Loc.: RERA basement Equipment Name: Reach-In Growth Chamber Performance Criteria:

Ambient building conditions: 60º F to 85º F, no RH control Minimum chamber temp required Lights on:10º C Lights off: 4º C Maximum chamber temp required Lights on:45º C Lights off: 45º C Uniformity of temperature required (defined on horizontal plane thru conditioned area.): +/- 0.5º C Minimum relative humidity required

Maximum relative humidity required Lights Off: 90% Lights On:85% Relative Humidity tolerance: +/- 3% Filtration for insects? Yes/ No: Mesh size required:

If yes – Kind of insect(s): Size of insect(s):

Filtration for dust: Yes/ No If yes, particle size removed:

Carbon dioxide control? No If yes, range:

Maximum light intensity required 875 300 uMol/sq m/s What distance from lamps:

Source of light used Fluorescent tubes with dimming ballasts and incandescent lamps.

Step lighting if dimming not available.

ATC26 not dimmable.

Barrier or open lamp canopy:

Levels of light control required Preference for airflow direction Horizontal Automatic watering required No If yes, describe:

Number of 120V, 15amp receptacles: * 1/2 Of Unit can be turned off? No 2 separate chambers? No Size Consideration:

Reach-In or Walk-In and max size: Reach-in 95” x 35” x 78” High Growth area: 26 SF Growth height: 24” for Arabidopsis Shelving in room: 1 shelf Size of shelves: 1 at ___” x ___”

1 at __” x __” Number of tiers: 2

Move-in path restrictions: Max width: 70” Max Height: 79” Install location vertical space: 10’-7” (field-verify) Building Services Required:

Est. electrical service required: 120/208volt, 3 phase, 60 hz, 30 amp

4 wire plus ground Single Point of connection: yes

Cooling water: gpm: 6.0 Entering water temp: 85º F Heat rejected to cooling water: BTU/hr: 30,000 basis of design Leaving water temp: 95º F Demineralized humidification water: Maximum Flow: 1.1 US gal/hr Quality required: pH 7.0 +/- 0.5

Resistivity: 0.5 to 1.0 Meg Ohms Filtration: less than 2 microns

Compressed air: None Pressure: Dewpoint: SCFM:

Drains Connection: 1” diameter

EQUIPMENT NO. B-4 (pg 2 of 2)

Refrigerant Type: R-134a, R-404A or approved equal Refrigerant Charge (lbs):

Number of circuits: * Number of compressors * Compressor type: * Number of cooling stages: * Number of heating stages: * Capacity control type: * Refrigerant metering device: * Growth Chamber Indoor Coil:

Indoor coil material: * Indoor coil number of rows/fins: * Chamber indoor coil airflow:* Blower motor HP: * Blower size and rpm:* Cooling capacity (btu/hr): * Heating capacity (btu/hr): * Water Cooled Condensing unit: Mounted on chamber B-4 Water flow (gpm): * Variable or constant flow: Constant Entering water temp maximum (ºF): 85 Entering water temp minimum (ºF): 45 Water pressure drop (feet of water): * Control System:

User interface: Color touch screen with scalability Alarm: Audible and color coded visual alarms, automatic shutoff at high temperature

User settable alarm points for all controlled processes.

Internet connectivity: yes Programmability: Up to 16 real-time user entered trend graph displays up to 5 days of main chamber processes

Chamber inputs and outputs logged to daily text files retrievable from Ethernet connection.

Service diagnostic capability: yes

Security: Password protection for administrator and three users

Start-up delay: Programmable variable start up times after power failure to reduce start-up loads

REMARKS: Unit B-4 is incorrectly shown on Drawing Q300-R as a Model ATC60. It should be shown as Model ATC26.

Prepared by: Rev. No. Rev. by Date Approved Date:

Checked by:

Approved:

DATA SHEET – GROWTH ROOM

Client: U.S. Dept of Agriculture Project No.: 25KT8810 Equip. No.: B-5 (pg 1 of 2)

Manufacturer: Conviron or equal Sys. Spec. No.: 13040 Plant Loc.: Albany, California Basis of design Model #: C4010 Quantity: 1 Equip. Loc.: RERA Basement Equipment Name: 3-Room Walk-In Growth Chamber with Lightlock Vestibule Performance Criteria:

Ambient building conditions: 60º F to 85º F, no RH control Is independent temp and RH control required in each of the three rooms?

Yes West Room: 4 to 40 deg C Center Room: 10 to 40 deg C East Room: 10 to 40 deg C

Minimum chamber temp required Lights on:10º C Lights off: 4º C Maximum chamber temp required Lights on:45º C Lights off: 45º C Uniformity of temperature required (defined on horizontal plane thru conditioned area.): +/- 0.5 1.0 deg C Minimum relative humidity required

Maximum relative humidity required Lights Off: 90% Lights On:85% Relative Humidity tolerance: +/- 3% Ventilation/occupancy requirements: Number of people:

Filtration for insects? Yes / No Mesh size required:

Filtration for dust: Yes/ No If yes, particle size removed:

Carbon dioxide control? No If yes, range:

Maximum light intensity required 100 footcandles, 40” AFF

300 uMol/sq m/s at 22-24”shelf ht What distance from lamps:

Source of light used Fluorescent Barrier or open lamp canopy?

Levels of light control required Step lighting, not dimmable

Programmable, not dimmable, to match existing B-5

Preference for airflow direction Automatic watering required No If yes, describe:

Number of 120V, duplex receptacles: 4 per roomAs required for lights Receptacle current rating: 15 amps 1/2 Of Unit can be turned off? 3 rooms independently controlled.

Ramp for access with plant carts? Yes Floor drain required for spilled water? Yes Size Consideration:

Reach-In or Walk-In and max size: Walk-in

419” x 164” x 103” High + vestibule See plan drawing for requirements.

Growth area: *Maximize in space available Growth height: Arabadopsis Shelving in room: 1 workbench and 6 shelves/room

See sketches B-5-1 and B-5-1A

Move-in path restrictions: Max width: 70” Max Height: 79” Install location vertical space: 10’-7” (field-verif) Building Services Required:

Est. electrical service required: 120/208volt, 3 phase, 60 hz, 30 amp

4 wire plus ground per room Single Point of connection: Yes, 3 ea

Cooling water: gpm: 6 to each room Entering water temp: 85º F Heat rejected to cooling water: BTU/hr: 30,000 basis of design/room Leaving water temp: 95º F Demineralized humidification water: Maximum Flow: 1.1 US gal/hr/ room is basis of design Quality required: pH 7.0 +/- 0.5 Resistivity: 0.5 to 1.0 Meg Ohms Filtration: less than 2 microns

EQUIPMENT NO. B-5 (pg 2 of 2)

Compressed air: None Pressure: Dewpoint: SCFM:

Drains Connection: 1” diameter

Refrigeration: Unit mounted condensing unit.

Refrigerant Type: R-134a, R-404A or approved equal Refrigerant Charge (lbs):

Number of circuits: * Number of compressors * Compressor type: * Number of cooling stages: * Number of heating stages: * Capacity control type: * Refrigerant metering device: * Growth Chamber Indoor Coil:

Indoor coil material: * Indoor coil number of rows/fins: * Chamber indoor coil airflow:* Blower motor HP: * Blower size and rpm:* Cooling capacity (btu/hr): * Heating capacity (btu/hr): * Water Cooled Condensing unit: Mounted on Unit B-5 Water flow (gpm): * Variable or constant flow: Constant Entering water temp maximum (ºF): 85 Entering water temp minimum (ºF): 45 Water pressure drop (feet of water): * Control System:

User interface: Color touch screen with scalability Alarm: Audible and color coded visual alarms, automatic shutoff at high temperature

User settable alarm points for all controlled processes.

Internet connectivity: yes Programmability: Up to 16 real-time user entered trend graph displays up to 5 days of main chamber processes

Chamber inputs and outputs logged to daily text files retrievable from Ethernet connection.

Service diagnostic capability: yes

Date:

Checked by:

Approved:

Client: U.S. Dept of Agriculture Project No.: 25KT8810 Equip. No.: B-6A, B-12 (pg 1 of 2)

Manufacturer: Conviron or equal Sys. Spec. No.: 13040 Plant Loc.: Albany, California Basis of design Model #: ATC40 Quantity: 2 Equip. Loc.: RERA basement Equipment Name: REACH-IN GROWTH CHAMBER

Performance Criteria:

Ambient building conditions: 60 deg F to 85 deg F, no RH control Minimum chamber temp required Lights on:10 deg C Lights off: 4 deg C Maximum chamber temp required Lights on:45 deg C Lights off: 45 deg C Uniformity of temperature required (defined on horizontal plane thru conditioned area.): +/- 0.5 deg C Minimum relative humidity required Maximum relative humidity required Lights Off: 90% Lights On:85% Relative Humidity tolerance: +/- 3% Filtration for insects? Yes / No Mesh size required:

If yes – Kind of insect(s): Size of insect(s):

Filtration for dust: Yes/ No If yes, particle size removed:

Carbon dioxide control? No If yes, range:

Maximum light intensity required 300 uMol/sq m/s What distance from lamps:

Source of light used Fluorescent tubes with dimming ballasts and incandescent lamps.

Step control if dimming not avail.

Provide specular aluminum endwalls

Levels of light control required 3 steps Preference for airflow direction Yes If yes, advise: horizontal Automatic watering required Yes If yes, describe: Tray type ebb & flow Size Consideration:

Reach-In or Walk-In and max size: Reach in 100” x 35.5” x 101” High Growth area: 38 square feet Growth height: 24 inches for Arabidopsis Shelving in room: Size of shelves: 1 at ___” x ___”

Building Services Required:

Est. electrical service required: 120/208volt, 3 phase, 60 hz, 30 amp

4 wire plus ground Single Point of connection: yes

Cooling water: gpm: 5.2 Entering water temp: 85º F Heat rejected to cooling water: BTU/hr: 26,000 Leaving water temp: 95º F Demineralized humidification water: Maximum Flow: 1.1 US gal/hr Quality required: Ph 7.0 +/- 0.5

Resistivity: 0.5 to 1.0 Meg Ohms Filtration: less than 2 microns

Compressed air: None Pressure: Dewpoint: SCFM:

Drains Connection: 1” diameter

Potable water for plant watering: Yes

EQUIPMENT NO. B-6A, B-12 (pg 2 of 2)

Refrigerant Type: R-134a, R-404A or approved equal Refrigerant Charge (lbs):

Number of circuits: * Number of compressors * Compressor type: * Number of cooling stages: * Number of heating stages: * Capacity control type: * Refrigerant metering device: * Growth Chamber Indoor Coil:

Indoor coil material: * Indoor coil number of rows/fins: * Chamber indoor coil airflow:* Blower motor HP: * Blower size and rpm:* Cooling capacity (btu/hr): * Heating capacity (btu/hr): * Water Cooled Condensing Unit: Mounted on chamber B-6A, B-12 Water flow (gpm): * Variable or constant flow: Constant Entering water temp maximum (ºF): 85 Entering water temp minimum (ºF): 45 Water pressure drop (feet of water): * Control System:

User interface: Color touch screen with scalability Alarm: Audible and color coded visual alarms, automatic shutoff at high temperature

User settable alarm points for all controlled processes.

Internet connectivity: yes Programmability: Up to 16 real-time user entered trend graph displays up to 5 days of main chamber…