Attachment_8_-_Appendix_3_IFGS_DW_test_methods.pdf

ITALY - 3 DrinkingWater

September 2012 Italy FGS 3-41 Revision 2

Table 3.25. Parameters with Specified Analytical Method

Parameter Methodology

ISS1

Analytical Method Code

Escherichia coli (E. coli) MPN (Most Probable Number)

Membrane Filtration

Membrane Filtration

ISS A 001A rev.00

ISS A 001B rev.00

ISS A 001C rev.00 Enterococci Membrane Filtration ISS A 002A rev.00 Pseudomonas aeruginosa Membrane Filtration ISS A 003A rev.00 Colony count 22 °C Plate Culture (Agar) ISS A 004A rev.00

Clostridium Perfringens (including spores)

Membrane Filtration

Membrane Filtration

ISS A 005A rev.00

ISS A 005B rev.00

Coliforms (37°C)

MPN (Most Probable Number)

Membrane Filtration

Membrane Filtration

ISS A006A rev.00

ISS A 006B rev.00

ISS A 006C rev.00

Algae Sedimentation with microscopic technique

ISS A 007A rev.00

Anti-E. coli Bacteriophages

MPN (Most Probable Number)

Plaque Count (lysis)

Presence/Absence

ISS A 008A rev.00

ISS A 008B rev.00

ISS A 008C rev.00

F-specific RNA Bacteriophages Plaque Assay (lysis) ISS A 009A rev.00

Free-living Nematodes

Sedimentation with microscopic technique

Membrane Filtration with microscopic technique

ISS A 0010A rev.00

ISS A 0010B rev.00

Salmonella

Presence/Absence

Presence/Absence

Presence/Absence

ISS A 0011A rev.00

ISS A 0011B rev.00

ISS A 0011C rev.00

Shigella

Presence/Absence

Presence/Absence

Presence/Absence

ISS A 0012A rev.00

ISS A 0012B rev.00

ISS A 0012C rev.00

Vibrio Presence/Absence ISS A 0013A rev.00

Aeromonas Membrane Filtration ISS A 0014A rev.00

Enterovirus Concentration and Isolation on Cellulose Culture, Immunofluorescence, ELISA, PCR

ISS A 0015A rev.00

Fungi Membrane Filtration ISS A 0016A rev.00

APPENDIX #3

September 2012 Italy FGS 3-42 Revision 2

Parameter Methodology

ISS1

Analytical Method Code

Membrane Filtration

Membrane Filtration

ISS A 0016B rev.00

ISS A 0016C rev.00

Giardia and Cryptosporidium

Capsule Filtration and Direct Immunofluorescence

Compressed Foam Filtration and Direct Immunofluorescence

PCR and Nested-PCR

Real-Time PCR

ISS A 0017A rev.00

ISS A 0017B rev.00

ISS A 0017C rev.00

ISS A 0017D rev.00

Pathogenic Staphylococci Membrane Filtration

Membrane Filtration

ISS A 0018A rev.00

ISS A 0018B rev.00

Note:

1. The composition of m-CP agar is:

Basal medium Tryptose 30 g Yeast Extract 20 g Sucrose 5 g L-Cystein Hydrochloride 1 g MgSO4 * 7 H2O 0.1 g Bromocresol purple 40 mg Agar 15 g Water 1,000 mL

Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121 °C (250 °F) for 15 minutes. Allow the medium to cool and add:

D-cycloserine 400 mg Polymixine – B sulfate 25 mg before addition 60 mg

Filter-sterilized 0.5 % phenolpthalein diphosphate solution 20 mL Filter-sterilized 4.5% FeCl3 *6 H2O 2 mL

September 2012 Italy FGS 3-43 Revision 2

Table 3.26. Parameters For Which Performance Characteristics Are Specified

Parameter Accuracy

(Trueness) % of

MCL (1)

Precision % of

MCL (2)

Limit of detention % of MCL (3)

Acrylamide To be controlled by product specification Aluminum 10 10 10 Ammonium 10 10 10 Antimony 25 25 25 Arsenic 10 10 10 Benzo[a]pyrene 25 25 25 Benzene 25 25 25 Boron 10 10 10 Bromate 25 25 25 Cadmium 10 10 10 Chloride 10 10 10 Chromium 10 10 10 Conductivity 10 10 10 Copper 10 10 10 Cyanide (4) 10 10 10 1,2 dichloroethane 25 25 10 Epichlorohydrin To be controlled by product specification

Fluoride 10 10 10 Hydrogen ion concentration The method of analysis used must be capable of measuring concentrations equal to the MCL with a trueness of 0.2 pH unit and a precision of 0.2 pH unit.

Iron 10 10 10 Lead 10 10 10 Manganese 10 10 10 Mercury 20 10 20 Nickel 10 10 10 Nitrate 10 10 10 Nitrite 10 10 10 Oxidizability (5) 25 25 10 Pesticides (6) 25 25 25 Polycyclic aromatic hydrocarbons (7)

25 25 25

Selenium 10 10 10 Sodium 10 10 10 Sulfate 10 10 10 Tetrachloroethene (8) 25 25 10 Trichloroethene (8) 25 25 10 Trialomethanes Total (7) 25 25 10 Vinyl Chloride To be controlled by product specification

September 2012 Italy FGS 3-44 Revision 2

Notes:

1. Trueness is the systematic error and is the difference between the mean value of the large number of repeated measurements and the true value.

2. Precision is the random error and is usually expressed as the standard deviation (within and between batch) of the spread of results about the mean.

3. Limit of detection is either:

- three times the relative within batch standard deviation of a natural sample containing a low concentration of the parameter, or

- five times the relative within batch standard deviation of a blank sample.

4. The method should determine total cyanide in all forms.

5. Oxidation should be carried out for 10 minutes at 100 °C (212°F) under acid conditions using permanganate.

6. The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned.

The limit of detection may not be achievable for all pesticides at present, but it should strive to achieve this standard.

7. The performance characteristics apply to the individual substances specified at 25 % of the MCL in Table 3.6.

8. The performance characteristics apply to the individual substances specified at 50 % of the MCL in Table 3.6.

September 2012 Italy FGS 3-45 Revision 2

Table 3.27. Italian Analytical Test Methods

Parameter Method

ISS 1, 2

Analytical Method Code

Acrylamide Quantitative determination, or Mass spectrometry Liquid chromatography

ISS XAA 001 rev.00 ISS CBA 001 rev.00

Aluminum Atomic absorption spectroscopy, or Inductively Coupled Plasma

ISS DAA 018 rev.00 ISS DBA 035 rev.00

Ammonium Spectrophotometric ISS BHE 019 rev.00 Antimony Atomic absorption spectroscopy or

Inductively Coupled Plasma - Hydrides

ISS DAA 002 rev.00 ISS DBB 034 rev.00

Arsenic Atomic absorption spectroscopy, or Inductively Coupled Plasma - Hydrides

ISS DAA 003 rev.00 ISS DBB 034 rev.00

Barium Atomic absorption spectroscopy ISS DAA 046 rev.00 Benzo(a)pyrene Gas chromatography / Mass spectrometry and Gas Chromatography/Flame ionization detector

ISS CAB 039 rev.00

Benzene Gas chromatography (headspace static), or Gas Chromatography (Headspace dynamic)

ISS CAA 004 rev.00 ISS CAD 004 rev.00

Boron Spectrophotometry (curnumin), or Spectrometry (azomethin), or Inductively Coupled Plasma

ISS BHA 005 rev.00 ISS BHB 005 rev.00 ISS DBA 035 rev.00

Bromate Ion exchange chromatography ISS CBB 006 rev.00 Cadmium Atomic absorption spectroscopy, or

Inductively Coupled Plasma ISS DAA 007 rev.00 ISS DBA 035 rev.00

Chloride Argentometry, or Ion exchange chromatography

ISS BEA 020 rev.00 ISS CBB 037 rev.00

Chlorite Ion exchange chromatography ISS CBB 037 rev.00 Chromium Atomic absorption spectroscopy, or

Inductively Coupled Plasma ISS DAA 008 rev.00 ISS DBA 035 rev.00

Cobalt Atomic absorption spectroscopy ISS DAA 048 rev.00

Color Photometric method (Platinum- Cobalt)

ISS BJA 021 rev.00

Conductivity Conductometry ISS BDA 022 rev.00 Copper Atomic absorption spectroscopy, or

Inductively Coupled Plasma ISS DAA 009 rev.00 ISS DBA 035 rev.00

Cyanide Spectrophotometry ISS BHC 010 rev.00 Cyanotoxins (microcystins) Immunoassay, or

Liquid chromatography/ Mass spectrometry

ISS BGA 044 rev.00 ISS CBA 044 rev.00

September 2012 Italy FGS 3-46 Revision 2

Parameter Method

ISS 1, 2

Analytical Method Code

1,2 dichloroethane Gas chromatography (head space) ISS CAA 036 rev.00 Epichlorohydrin Quantitative determination ISS XAA 011 rev.00 Dry residue (180°C) Gavimetric ISS BFA 032 rev.00 Fluoride Ion exchange chromatography ISS CBB 037 rev.00 Hardness Titrimetric

(ethylenediaminetetraacetic acid) ISS BEC 031 rev.00

Iron Atomic absorption spectroscopy, or Inductively Coupled Plasma

ISS DAA 024 rev.00 ISS DBA 035 rev.00

Lead Atomic absorption spectroscopy, or Inductively Coupled Plasma

ISS DAA 012 rev.00 ISS DBA 035 rev.00

Manganese Atomic absorption spectroscopy, or Inductively Coupled Plasma

ISS DAA 025 rev.00 ISS DBA 035 rev.00

Mercury Atomic absorption spectrometry without flame (on cold steam)

ISS DAB 013 rev.00

Nickel Atomic absorption spectroscopy, or Inductively Coupled Plasma

ISS DAA 014 rev.00 ISS DBA 035 rev.00

NO3 Ion exchange chromatography ISS CBB 037 rev.00 NO2 Ion exchange chromatography ISS CBB 037 rev.00 Odor Sensory technique ISS BAA 026 rev.00 Oxidizability Titrimetric analysis (permanganate) ISS BEB 027 rev.00 Pesticides Gas chromatography/Mass spectrometry (solid phase extraction)

ISS CAC 015 rev.00 pH Potentiometric ISS BCA 023 rev.00 Polycyclic aromatic hydrocarbons

Gas chromatography/Mass spectrometry and Gas Chromatography/Flame ionization detector

ISS CAB 039 rev.00

Residual chlorine N,NDiethyl-p-phenylenediamine (DPD) colorimetric method

ISS BHD 033 rev.00

Selenium Atomic absorption spectroscopy or Inductively Coupled Plasma - Hydrides

ISS DAA 016 rev.00 ISS DBB 034 rev.00

Silver Atomic absorption spectroscopy ISS DAA 045 rev.00 Sodium Ion exchange chromatography, or

Inductively Coupled Plasma ISS CBB 038 rev.00

Sulfates Ion exchange chromatography ISS CBB 037 rev.00 Taste Sensory technique ISS BKA 028 rev.00- Tetrachloroethene Gas chromatography (headspace) ISS CAA 036 rev.00 Trichloroethene Gas chromatography (headspace) ISS CAA 036 rev.00 Trialomethanes Total Gas chromatography (headspace) ISS CAA 036 rev.00 Total organic carbon Instrumental method ISS BIA 029 rev.00

September 2012 Italy FGS 3-47 Revision 2

Parameter Method

ISS 1, 2

Analytical Method Code

Turbidity nephelometric (formazine) ISS BLA 030 rev.00 Undissolved Solids Gravemetric ISS BFA 042 rev.00 Vanadium Atomic absorption spectroscopy, or

Inductively Coupled Plasma ISS DAA 017 rev.00 ISS DBA 035 rev.00

Vinyl Chloride Quantitative determination ISS XAA 040 rev.00 Zinc Atomic absorption spectroscopy ISS DAA 049 rev.00 Notes:

1. For additional details, see manual published by ISS (Istituto Superiore di Sanita – Health Institute) titled “Reference analytical methods for water intended for human consumption – Chemical Methods (07/31).” A Component can approve or delegate approval of different test methods than indicated above if the alternate test method will attain the same limits in terms of detection, accuracy, and precision.

2. Procedures related to the analytical control of water destined for human consumption:

– Collection and preservation of samples = Follow Code ISS PGA 901 rev.00

– Quality Control = Follow Code ISS PGA 903 rev.00

September 2012 Italy FGS 3-48 Revision 2

Table 3.28. DoD-Approved Inorganic Chemical Analytical Test Methods

Parameter Methodology 13 EPA ASTM 3

SM 4

(18th, 19th ed.)

SM 4

(20th ed.)

SM

Online22 Other

Alkalinity Titrimetric D1067-92, 02 B

2320 B 2320 B 2320 B-97

Electrometric titration I-1030-855

Aluminum Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)

200.5 Revision 4.22

Atomic Absorption; Direct 3111 D Atomic Absorption; Furnace

Inductively Coupled Plasma (ICP)

3113B

3120 B

3113 -04, B-10

Antimony ICP-Mass Spectrometry 200.8 2

Hydride-Atomic Absorption D-3697-92, Atomic Absorption (Platform) 200.9 2

Atomic Absorption (Furnace) 3113 B 3113 B-99 Arsenic 14 ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.92 Atomic Absorption (Furnace) D-2972-03C 3113 B 3113 B-99 Hydride Atomic Absorption D-1972-03B 3114 B 3114 B-97

Asbestos Transmission Electron Microscopy 100.1 9

Transmission Electron Microscopy 100.210

Barium ICP 200.7 2 3120 B 3120 B 3120 B-99 ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Direct) 3111 D 3111 D-99 Atomic Absorption (Furnace) 3113 B 3113 B-99

Beryllium ICP 200.7 2 3120 B 3120 B 3120 B-99 ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Atomic Absorption (Furnace) D3645-97, 03B

3113 B 3113 B-99

Cadmium ICP 200.7 2

ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Atomic Absorption (Furnace) 3113 B 3113 B-99 Calcium EDTA Titrimetric D511-93, 03A 3500-Ca D 3500-Ca B 3500-Ca B-

Atomic Absorption (Direct Aspiration)

D511-93, 03B

3111 B 3111 B-99

Inductively-Coupled Plasma Ion Chromatography

200.7 2

D6919-03 3120 B 3120 B 3120 B-99

Chloride Silver Nitrate Titration D 512-04 B, 12 B

4500-Cl- B 4500-Cl- B

Ion Chromatography D4327-11 4110 B 4110 B Potentiometric Titration 4500-Cl-D 4500-Cl-D

Chromium ICP 200.7 2 3120 B 3120 B 3120 B-99 ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Atomic Absorption (Furnace) 3113 B 3113 B-99

September 2012 Italy FGS 3-49 Revision 2

Parameter Methodology 13 EPA ASTM 3

SM 4

(18th, 19th ed.)

SM 4

(20th ed.)

SM

Online22 Other

Color Visual Comparison 2120 B 2120 B 2120B-01 Copper Atomic Absorption (Furnace) D1688-95, 02 C 3113 B 3113 B-99

Atomic Absorption (Direct Aspiration)

D1688-95, 02 A

3111 B 3111 B-99

ICP 200.7 2 3120 B 3120 B 3120 B-99

ICP -Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Conductivity Conductance D1125-95A 2510 B 2510 B 2510 B-97 Cyanide Manual Distillation followed by: D2036-98A 4500-CN-

C

4500-CN-C

Spectrophotometric (Amenable) D2036-98B 4500-CN- G

4500-CN-G 4500-CN-

G-99

Spectrophotometric (Manual) D2036-98A 4500-CN- E

4500-CN-E 4500-CN-

E-99

I-3300-85

Spectrophotometric (Semi-automated)

335.4 6

Selective Electrode

UV, Distillation, Spectophotometric

Micro Distillation, Flow Injection, Spectrophotometric

Ligand Exchange and Amperometry21

D6888-04

4500-CN-

F

4500-CN-F 4500-CN-

F-99

Kelada-

QuikChe m 10-204- 00-1-X18

OIA-

1677, DW20

Foaming Agents

Methylene Blue Active Substances

(MBAS)

5540C 5540C 5540 C-00

Fluoride Ion Chromatography 300.0 6

300.119 D4327-97, 4110 B 4110 B 4110B-00

Manual Distill (Color SPADNS) 4500-F-B, D

4500-F-B, D

4500F-B- D-97

Manual Electrode D1179-93, 99 B

4500-F-C 4500-F-C 4500-F-C-

Automated Electrode 380-

75WE11

Automated Alizarin Capillary Ion Electrophoresis

4500-F-E 4500-F-E 4500-F-E-

129-71W

D6508, Rev. 223

Iron AVICP-AES 200.5, Revision

4.2 2

Atomic Absorption (Direct) 3111 B 3111 B Atomic Absorption (Furnace) 3111 B 3111 B 3111 B-04, B-10

ICP 3120 B 3120 B

September 2012 Italy FGS 3-50 Revision 2

Parameter Methodology 13 EPA ASTM 3

SM 4

(18th, 19th ed.)

SM 4

(20th ed.)

SM

Online22 Other

Lead Atomic Absorption (Furnace) D3559-96, 03 D

3113 B 3113 B-99

ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Differential Pulse Anodic Stripping Voltammetry

Method 100116

Magnesium Atomic Absorption D 511-93, 03 B

3111 B 3111 B-99

ICP 200.7 2 3120 B 3120 B 3120 B-99

Complexation Titrimetric Methods

Ion Chromatography

D 511-93, 03 A

D6919-03

3500-Mg E 3500-Mg B 3500-Mg- B-97

Manganese AVICP-AES 200.5, Revision

4.2 2

Atomic Absorption (Direct) 3111 B 3111 B Atomic Absorption (Furnace) 3111 B 3111 B 3111 B-04, B-10

ICP 3120 B 3120 B

Mercury Manual (Cold Vapor) 245.1 2 D3223-97, 3112 B 3112 B-99

Automated (Cold Vapor) 245.2 1

ICP-Mass Spectrometry 200.8 2

Nickel ICP 200.7 2 3120 B 3120 B 3120 B-99 ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Atomic Absorption (Direct) 3111 B 3111 B-99 Atomic Absorption (Furnace) 3113 B 3113 B-99

Nitrate Ion Chromatography 300.0 6

300.119 D4327-97, 4110 B 4110 B 4110 B-00 B-1011 8

Automated Cadmium Reduction 353.2 6 D3867-90A 4500-NO3

F

4500-NO3

F

4500-NO3

F-00 Ion Selective Electrode 4500-NO3

D

4500-NO3

D

4500-NO3

D-00

Manual Cadmium Reduction

Capillary Ion Electrophoresis

D3867-90B D6508-00

4500-NO3

E

4500-NO3

E

4500-NO3

E-00

Nitrite Ion Chromatography 300.0 6

300.119 D4327-97, 4110 B 4110 B 4110 B-00 B-1011 8

Automated Cadmium Reduction 353.2 6 D3867-90A 4500-NO3

F

4500-NO3

F

4500-NO3

F-00 Manual Cadmium Reduction D3867-90B 4500-NO3

E

4500-NO3

E

4500-NO3

E-00 Spectrophotometric

Capillary Ion Electrophoresis D6508-00

4500-NO2

B

4500-NO2

B

4500-NO2

B-00

Odor Threshold Odor Test 2150 B 2150 B 2150 B-97 Orthophospha te

Colorimetric (Automated, Ascorbic Acid)

365.1 6 4500-P F 4500-P F

Colorimetric (Ascorbic Acid, Single Reagent)

D515-88A 4500-P E 4500-P E

September 2012 Italy FGS 3-51 Revision 2

Parameter Methodology 13 EPA ASTM 3

SM 4

(18th, 19th ed.)

SM 4

(20th ed.)

SM

Online22 Other

Colorimetric (Phosphomolybdate) I-1602-85

Automated-Segmented Flow I-2601-90

Automated Discrete I-2598-85

Ion Chromatography

Capillary Ion Electrophoresis

300.0 6

300.119 D4327-97, D6508-00

4110 B 4110 B 4110 B-00 pH Electrometric 150.1 1 D1293-95, 4500-H+ B 4500-H+ B 4500-H+ B-

150.2 2

Selenium Hydride-Atomic Absorption D3859-98, - 03A

3114 B 3114 B-97

ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Atomic Absorption (Furnace) D3859-98, 03B

3113 B 3113 B-99

Silica Colorimetric, Molybdate Blue I-1700-85

Automated-Segmented Flow I-2700-85

Colorimetric D859-94, 00 Molybdosilicate 4500-Si D 4500-SiO2

C 4500-SiO2 C-97

Heteropoly blue 4500-Si E 4500-SiO2 D

4500-SiO2 D-97

Automated for Molybdate-reactive Silica

4500-Si F 4500-SiO2 E

4500-SiO2 E-97

ICP 200.72 3120 B 3120 B 3120 B-99

Silver AVICP-AES 200.5, Revision

4.2 2

Atomic Absorption (Direct) 3111 B 3111 B Atomic Absorption (Furnace) 3111 B 3111 B 3111 B-04, B-10

ICP 3120 B 3120 B

Sodium Inductively-Coupled Plasma 200.7 2

Atomic Absorption (Direct Aspiration) Ion Chromatography D6919-03

3111 B 3111 B-99

Sulfate Ion Chromatography D4327-11 4110 B 4110 B Gravimetric with ignition of residue

4500-SO4

2-

C

4500-SO4

2-

C

4500-SO4

2-

C-97 Gravimetric with drying of residue 4500-SO4

2-

D

4500-SO4

2-

D

4500-SO4

2-

D-97

September 2012 Italy FGS 3-52 Revision 2

Parameter Methodology 13 EPA ASTM 3

SM 4

(18th, 19th ed.)

SM 4

(20th ed.)

SM

Online22 Other

Turbidimetric method D 516-07, 4500-SO4

2-

E

4500-SO4

2-

E

4500-SO4

2-

E-97

Automatic methylthymol blue method

4500-SO4

2-

F

4500-SO4

2-

F

4500-SO4

2-

F-97 Temperature Thermometric 2550 2550 2550-00 Thallium ICP-Mass Spectrometry 200.8 2

Atomic Absorption (Platform) 200.9 2

Zinc AVICP-AES 200.5, Revision

4.2 2

Atomic Absorption (Direct Aspiration)

3111 B 3111 B

ICP 3120 B 3120 B

Notes:

The procedures shall be done in accordance with the documents listed below. Copies of the documents may be obtained from the sources listed below. Information regarding obtaining these documents can be obtained from the Safe Drinking Water Hotline at 800-426-4791. Documents may be inspected at EPA’s Drinking Water Docket, EPA West, 1301 Constitution Avenue, NW., Room 3334, Washington, DC 20460 (Telephone: 202-566-2426); or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202-741-6030, or go to:

http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html..

1. “Methods for Chemical Analysis of Water and Wastes’’, EPA/600/4-79/020, March 1983. Available at NTIS, PB84- 128677.

2. “Methods for the Determination of Metals in Environmental Samples--Supplement I’’, EPA/600/R-94/111, May 1994. Available at NTIS, PB95-125472.

3. Annual Book of ASTM Standards, ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428, http://www.astm.org.; Annual Book of ASTM Standards 1994, Vols. 11.01 and 11.02; Annual Book of ASTM Standards 1996, Vols. 11.01 and 11.02; Annual Book of ASTM Standards 1999, Vols. 11.01 and 11.02;

Annual Book of ASTM Standards 2003, Vols. 11.01 and 11.02.

4. Standard Methods for the Examination of Water and Wastewater, American Public Health Association, 800 I Street NW., Washington, DC 20001-3710; Standard Methods for the Examination of Water and Wastewater, 18th edition (1992); Standard Methods for the Examination of Water and Wastewater, 19th edition (1995);

Standard Methods for the Examination of Water and Wastewater, 20th edition (1998).The following methods from this edition cannot be used: 3111 B, 3111 D, 3113 B, and 3114 B.

5. U.S. Geological Survey, Federal Center, Box 25286, Denver, CO 80225-0425; Methods for Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediment, Open File Report 93-125, 1993; Techniques of Water Resources Investigation of the U.S. Geological Survey, Book 5, Chapter A-1, 3rd edition, 1989.

6. “Methods for the Determination of Inorganic Substances in Environmental Samples,” EPA/600/R-93/100, August 1993. Available at NTIS, PB94-120821.

7. The procedure shall be done in accordance with the Technical Bulletin 601 “Standard Method of Test for Nitrate in Drinking Water,” July 1994, PN 221890-001, Analytical Technology, Inc. Copies may be obtained from ATI Orion, 529 Main Street, Boston, MA 02129.

8. Method B-1011, “Waters Test Method for Determination of Nitrite/Nitrate in Water Using Single Column Ion Chromatography,” August 1987. Copies may be obtained from Waters Corporation, Technical Services Division, 34 Maple Street, Milford, MA 01757.

September 2012 Italy FGS 3-53 Revision 2

9. Method 100.1, “Analytical Method For Determination of Asbestos Fibers in Water,” EPA/600/4-83/043, EPA, September 1983. Available at NTIS, PB83-260471.

10. Method 100.2, “Determination of Asbestos Structure Over 10-μm In Length In Drinking Water,” EPA/600/R- 94/134, June 1994. Available at NTIS, PB94-201902.

11. Industrial Method No. 129-71W, “Fluoride in Water and Wastewater,” December 1972, and Method No. 380- 75WE, “Fluoride in Water and Wastewater,” February 1976, Technicon Industrial Systems. Copies may be obtained from Bran & Luebbe, 1025 Busch Parkway, Buffalo Grove, IL 60089.

12. Unfiltered, no digestion or hydrolysis.

13. Because MDLs reported in EPA Methods 200.7 and 200.9 were determined using a 2X preconcentration step during sample digestion, MDLs determined when samples are analyzed by direct analysis (i.e., no sample digestion) will be higher. For direct analysis of cadmium and arsenic by Method 200.7, and arsenic by Method 3120 B sample preconcentration using pneumatic nebulization may be required to achieve lower detection limits. Preconcentration may also be required for direct analysis of antimony, lead, and thallium by Method 200.9; antimony and lead by Method 3113 B; and lead by Method D3559-90D unless multiple in-furnace depositions are made.

14. If ultrasonic nebulization is used in the determination of arsenic by Method 200.8, the arsenic must be in the pentavalent state to provide uniform signal response. For direct analysis of arsenic with Method 200.8 using ultrasonic nebulization, samples and standards must contain 1 mg/L of sodium hypochlorite.

15. [Reserved]

16. The description for Method Number 1001 for lead is available from Palintest, LTD, 21 Kenton Lands Road, P.O. Box 18395, Erlanger, KY 41018. Or from the Hach Company, P.O. Box 389, Loveland, CO 80539.

17. The description for the Kelada-01 Method, “Kelada Automated Test Methods for Total Cyanide, Acid Dissociable Cyanide, And Thiocyanate,” Revision 1.2, August 2001, EPA # 821-B-01-009 for cyanide is available from the National Technical Information Service (NTIS), PB 2001-108275, 5285 Port Royal Road, Springfield, VA 22161. The toll free telephone number is 800-553-6847. Note: A 450-W UV lamp may be used in this method instead of the 550-W lamp specified if it provides performance within the quality control (QC) acceptance criteria of the method in a given instrument. Similarly, modified flow cell configurations and flow conditions may be used in the method, provided that the QC acceptance criteria are met.

18. The description for the QuikChem Method 10-204-00-1-X, “Digestion and distillation of total cyanide in drinking and wastewaters using MICRO DIST and determination of cyanide by flow injection analysis,” Revision 2.1, November 30, 2000, for cyanide is available from Lachat Instruments, 6645 W. Mill Rd., Milwaukee, WI 53218. Telephone: 414-358-4200.

19. “Methods for the Determination of Organic and Inorganic Compounds in Drinking Water,” Vol. 1, EPA 815-R- 00-014, August 2000. Available as Technical Report PB2000-106981 at National Technical Information Service (NTIS), 5301 Shawnee Road, Alexandria, VA 22312. http://www.ntis.gov.

20. Method OIA-1677, DW “Available Cyanide by Flow Injection, Ligand Exchange, and Amperometry,” January 2004. EPA-821-R-04-001, Available from ALPKEM, A Division of OI Analytical, P.O. Box 9010, College Station, TX 77842-9010.

21. Sulfide levels below those detected using lead acetate paper may produce positive method interferences. Test samples using a more sensitive sulfide method to determine if a sulfide interference is present, and treat samples accordingly.

22. Standard Methods Online, American Public Health Association, 800 I Street NW., Washington, DC 20001, available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.

September 2012 Italy FGS 3-54 Revision 2

Table 3.29. DoD-Approved Organic Chemicals Analytical Test Methods

Parameter Method

EPA Standard ASTM Other Alachlor2 507, 525.2, 508.1, 505,

551.1 Atrazine 2 507, 525.2, 508.1, 505,

551.1 Syngenta5 AG-625

Benzene 502.2, 524.2 Benzo[a]pyrene 525.2, 550, 550.1 Carbofuran 531.1, 531.2 6610 Carbon tetrachloride (Tetrachloromethane)

502.2, 524.2, 551.1

Chlordane 508, 525.2, 508.1, 505 Monochlorobenzene (Chlorobenzene)

502.2, 524.2

2,4-D4 (as acid, salts and esters) 515.2, 555, 515.1, 515.3, 515.4

D5317-93, 98

Dalapon 552.1, 515.1, 552.2, 515.3, 515.4, 552.3

Dibromochloropropane (DBCP) 504.1, 551.1 o-Dichlorobenzene (1,2- Dichlorobenzene)

502.2, 524.2 p-Dichlorobenzene (1,4- Dichlorobenzene)

502.2, 524.2

1,2-Dichloroethane 502.2, 524.2 1,1-Dichloroethylene (1,1- Dichloroethene)

502.2, 524.2 cis-Dichloroethylene (cis-1,2- Dichloroethene)

502.2, 524.2 trans-Dichloroethylene (trans- 1,2-Dichloroethene)

502.2, 524.2

Dichloromethane 502.2, 524.2 1,2-Dichloropropane 502.2, 524.2 Di(2-ethylhexyl)adipate 506, 525.2 Di(2-ethylhexyl)phthalate 506, 525.2 Dinoseb 4 515.2, 555, 515.1, 515.3,

515.4 Dioxin (2,3,7,8-TCDD) 1613 Diquat 549.2 Endothall 548.1 Endrin 508, 525.2, 508.1, 505,

551.1 Ethylbenzene 502.2, 524.2 Ethylene dibromide (EDB) (1,2- Dibromoethane)

504.1, 551.1

Glyphosate 547 6651 Heptachlor 508, 525.2, 508.1, 505,

551.1 Heptachlor Epoxide 508, 525.2, 508.1, 505,

551.1 Hexachlorobenzene 508, 525.2, 508.1, 505,

551.1 Hexachlorocyclopentadiene 508, 525.2, 508.1, 505, September 2012 Italy FGS 3-55 Revision 2

Parameter Method

EPA Standard ASTM Other 551.1

Lindane 508, 525.2, 508.1, 505, 551.1

Methoxychlor 508, 525.2, 508.1, 505, 551.1

Oxamyl 531.1, 531.2 6610 PCBs3 (as decachlorobiphenyl) (as Aroclors)

508A 508.1, 508, 525.2, 505

Pentachlorophenol 515.2, 525.2, 555, 515.1, 515.3, 515.4

D5317-93, 98

Picloram4 515.2, 555, 515.1, 515.3, 515.4

D5317-93, 98

Silvex (2,4,5-TP 4) 515.2, 555, 515.1, 515.3, 515.4

D5317-93, 98

Simazine 2 507, 525.2, 508.1, 505, 551.1

Styrene 502.2, 524.2 Tetrachloroethylene (Tetrachloroethene)

502.2, 524.2, 551.1

Toluene 502.2, 524.2 Total Trihalomethanes 502.2, 524.2, 551.1 Toxaphene 508, 508.1, 525.2, 505 1,2,4-Trichlorobenzene 502.2, 524.2 1,1,1-Trichloroethane 502.2, 524.2, 551.1 1,1,2-Trichloroethane 502.2, 524.2, 551.1 Trichloroethylene (Trichloroethene)

502.2, 524.2, 551.1

Vinyl chloride 502.2, 524.2 Xylenes (total) 502.2, 524.2

Notes:

1. [Reserved]

2. Substitution of the detector specified in Method 505, 507, 508 or 508.1 for the purpose of achieving lower detection limits is allowed as follows. Either an electron capture or nitrogen phosphorous detector may be used provided all regulatory requirements and quality control criteria are met.

3. PCBs are qualitatively identified as Aroclors and measured for compliance purposes as decachlorobiphenyl. Users of Method 505 may have more difficulty in achieving the required detection limits than users of Methods 508.1, 525.2 or 508.

4. Accurate determination of the chlorinated esters requires hydrolysis of the sample as described in EPA Methods 515.1, 515.2, 515.3 and 555, and ASTM Method D 5317-93.

5. This method may not be used for the analysis of atrazine in any system where chlorine dioxide is used for drinking water treatment. In samples from all other systems, any result for atrazine generated by Method AG-625 that is > one-half the maximum contaminant level (MCL) (in other words, > 0.0015mg/L or 1.5 volume of the original sample collected for compliance monitoring. In instances where a result from Method AG-625 triggers such confirmatory testing, the confirmatory result is to be used to determine compliance.