Attachment J Sampling and Testing Results_opt.pdf

VOLUME I

Technical Report on the Sampling and Testing of Material from Port Jersey Federal Navigation Channel, Proposed for Dredging and HARS Ocean Disposal

Contract No. W912DS-22-D-0002 Delivery Order No. W912DS-23-F-0012

Prepared for:

Army Corps of Engineers, New York District 26 Federal Plaza

New York, NY 10278

October 31, 2023

ASI Job No. 43-011

TABLE OF CONTENTS

VOLUME I, Executive Summary

Signature Page Summary Page I. Introduction A. Project Overview

B. Relevant Correspondence C. Project Deviations Summary

II. Test Administration A. Sponsor B. Testing Facilities C. Dates of Experiment D. Study Participants

III. Materials and Methods A. Sampling B. Homogenizing and Compositing C. Chemical Analyses D. Physical Analysis and Total Organic Carbon E. Solid Phase Testing F. Suspended Particulate Phase Testing G. Bioaccumulation Testing

IV. Results and Discussion A. Solid Phase Testing B. Suspended Particulate Phase Testing C. Bioaccumulation Testing

V. References

FIGURES AND TABLES

Figure 1 Site Map Table 1a Sample Identification and Compositing Scheme Table 1b Sample Identification- Reference and Control Sediments Table 2 DGPS Coordinates- Core Locations and Site Waters Table 3 Test Performance Summary (% Survival) Table 4a Grain Size Distribution, Percent Moisture and TOC Values of Test Sediments and Composites Table 4b Grain Size Distribution, Percent Moisture and TOC Values of Reference and Control Sediments Table 5 Specific Gravity, Bulk Density, and Atterberg Limits Table 6 Sampling Summary and Holding Times

Water Quality Parameter Tables

Solid Phase A. abdita Static Non-Renewal Bioassay

Table 7 Live Count Table 8 Temperature Table 9 Salinity Table 10 D.O Table 11 pH Table 12 Pore Water Ammonia

Solid Phase A. bahia Static Non-Renewal Bioassay Table 13 Live Count Table 14 Temperature Table 15 Salinity Table 16 D.O Table 17 pH Table 18 Overlaying Ammonia Table 19 Unionized Ammonia

Suspended Particulate Phase M. beryllina Bioassay

Table 20 Live Count Table 21 Temperature Table 22 Salinity Table 23 D.O Table 24 pH

Suspended Particulate Phase A. bahia Bioassay

Table 25 Live Count Table 26 Temperature Table 27 Salinity Table 28 D.O Table 29 pH

Suspended Particulate Phase M. galloprovincialis Bioassay

Table 30 LC50 for Survival Table 31 EC50 for Development Table 32 Temperature Table 33 Salinity Table 34 D.O Table 35 pH

Bioaccumulation T. japonica Bioassay Table 36 Live Count Table 37 Temperature Table 38 Salinity Table 39 D.O Table 40 pH

Bioaccumulation A. virens Bioassay Table 41 Live Count Table 42 Temperature Table 43 Salinity Table 44 D.O Table 45 pH

VOLUME II, APPENDICES A – H

Appendix A: Sediment Core Logs Appendix B: Photographs Appendix C: Chains of Custody Appendix D: Sample Use Forms Appendix E: EA Lab Water Chemical Analyses Appendix F: Percent Moisture & Grain Size Distribution Raw Data Appendix G: Bulk Density, Specific Gravity, and Atterberg Limits Appendix H: Total Organic Carbon Raw Data

VOLUME III, APPENDICES I – J

Appendix I: EA - Ecotoxicological Testing Whole Sediment Bioassays Report and Raw Data

Appendix J: EA - Ecotoxicological Testing Water Column Bioassays Report and Raw Data

Appendix K: EA - Ecotoxicological Testing Toxicity and Bioaccumulation Assays Report and Raw Data

VOLUME IV, Public Notice Tables

Table 1 Chemical Analysis of Site Water and Elutriate Table 2 Toxicity Test Results Table 3 Chemical Analysis of Tissue Table 4 Supermemo

VOLUME V, Statistical Analysis of Tissue Samples: T. japonica

VOLUME VI, Statistical Analysis of Tissue Samples: A. virens

Summary Page

Technical Report on the Sampling and Testing of Material from Port Jersey Federal Navigation Channel, Proposed for Dredging and HARS Ocean Disposal

Contract No. W912DS-22-D-0002 Delivery Order No. W912DS-23-F-0012

Study Initiation Date

January 17, 2023

Study Completion Date

October 31, 2023

Performing Laboratory

Aqua Survey, Inc.

469 Point Breeze Road

Flemington, New Jersey 08822

Sponsor

Army Corps of Engineers, New York District

26 Federal Plaza New York, NY 10278

Laboratory Project ID

ASI Study No. 43-011

I. INTRODUCTION

A. Project Overview

The objective of this study was to perform sampling, compositing, physical characterization, and biological testing of sediment proposed for dredging from the Port Jersey Federal Navigation Channel.

Testing was performed in accordance with USEPA and USACE, 1991 Green Book, Evaluation of Dredged Material Proposed for Ocean Disposal – Testing Manual, EPA-503/8-91/001; and USACE New York District and USEPA Region 2, Guidance for Performing Tests on Dredged Material Proposed for Ocean Disposal, April 2016 [USEPA/USACE, 1991; USEPA/USACE-NYD, 2016].

Aqua Survey, Inc. (ASI) performed all sampling and physical analyses (grain size, percent moisture, TOC, bulk density, specific gravity and Atterberg Limits).

EA Engineering, Science, and Technology, Inc., PBC (EA) in Hunt Valley, MD, performed all biological testing.

Battelle and its subcontract laboratories, Cape Fear Analytical and Enthalpy Analytical, performed the required chemical analyses for the sediment, site water/elutriate and tissue samples.

Whole sediment toxicity was assessed through 10-day exposures with the mysid shrimp, Americamysis bahia, and the amphipod, Ampelisca abdita, in solid phase tests.

Toxicity of the elutriates prepared from the composite was assessed through 96-hour suspended particulate phase toxicity bioassays with the inland silverside, Menidia beryllina, and the mysid shrimp, Americamysis bahia. A 48-hour embryo development test was performed using the blue mussel, Mytilus galloprovincialis.

Bioaccumulation of metals, pesticides, PCB congeners, semivolatiles (1,4 dichlorobenzene), PAHs, and dioxin/furans was assessed using 28-day exposures of the clam, Tapes japonica and the sand worm, Alitta (formerly Nereis) virens to the composite sediment sample.

It should be noted that due to a supplier shortage of the Macoma nasuta, the alternate clam species, T. japonica, was approved to be used for testing by Jim Ferretti of USEPA, Region 2.

B. Relevant Correspondence

The following pages provide relevant correspondence for this project.

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CONTRACTING / ORDERING OFFICER

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BY: NICHOLAS P EMANUEL

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Aqua Survey, Inc. Jon Doi, Executive Vice President 2022Dec14

C. Project Deviations Summary

There were no project deviations for this project.

II. TEST ADMINISTRATION

A. Sponsor

Army Corps of Engineers, New York District (USACE-NYD) 26 Federal Plaza

B. Testing Facilities

Aqua Survey, Inc.

469 Point Breeze Road Flemington, NJ 08822

Battelle 141 Longwater Drive, Suite 202 Norwell, MA 02061

Cape Fear Analytical, LLC 3306 Kitty Hawk Road, #120 Wilmington, NC 28405

EA Engineering, Science, and Technology, Inc., PBC 231 Schilling Circle Hunt Valley, MD 21031

Enthalpy Analytical LLC 1941 Reymet Road Richmond, Virginia, 23237

C. Dates of Experiment

Date of Study Initiation: January 17, 2023 Date of Study Completion: October 31, 2023

D. Study Participants

Jon Doi, Ph.D. Executive Vice President Matthew Dickson Field Operations Support Michael Dodd Field Operations Support Thomas Dolce Field Operations Manager Cheryl Hall Quality Assurance Officer Elizabeth Horn Staff Scientist Ramiz Nseir Laboratory Support

Willard Reasoner Field Operations Specialist Michelle Thomas Laboratory Manager Paul Wood Field Operations Support Anthony Zanzonico Field Operations Specialist

III. MATERIALS AND METHODS

All sampling and testing was performed according to the sediment sampling and testing plan on file with the USACE-NYD.

A. Sampling

Test sediment from twenty-one locations and site water were collected from the Port Jersey Federal Navigation Channel, by Aqua Survey personnel on January 17, 18 and 20, 2023. Site water was collected on February 6, 2023.

The vessel used for sampling, the R/V Prattis, were positioned using a Trimble Differential Global Positioning System (DGPS). Sediment collection was performed using a Rossfelder P-3 vibracore with flexible plastic core liners. The project depth was minus 52.0 feet MLLW, including 2 foot overdredge.

See Table 2 for sample identification and DGPS coordinates. See Figure for the sampling locations map.

Each core sampled was inspected, photographed and its characteristics were recorded on a sediment core log. Sediment core logs are provided in Volume II (Appendix A) and photographs are provided in Volume II (Appendix B).

Upon arrival at ASI, all samples were logged in and assigned a unique sample number (Tables 1a and 1b). Samples were received at ASI in Flemington, NJ, under chain of custody and stored at 2◦C to 4◦C until testing. All supporting documentation, including chains-of-custody and sample use forms, can be found in Volume II (Appendices A-H) and Volume III (Appendices I-M).

On January 19, 2023, the Mud Dump Reference sediment (ASI ID 20230002/ EA ID AT3-073) was collected by ASI personnel from the ACOE-designated area six miles off the coast of Sandy Hook, NJ, (sampling coordinates:

40.33030 N, -73.87810 W).

B. Homogenizing and Compositing

Each sediment sample was carefully homogenized using a stainless steel mixer following specific guidelines found on pages 9-11 and Appendix A of the Dredging Manual and according to ASI’s standard operating procedure SOP/PRP/008. Samples were mixed until uniform in color and texture. Sample identification numbers and the compositing scheme are provided in Table 1.

An 800 ml subsample from each composite was archived, as required by the Corps, for possible future analysis.

C. Chemical Analyses

Subsamples of each composite and site waters were shipped by ASI to Battelle, Norwell, MA, for chemical analysis. Following chain-of-custody procedures, samples were placed in glass jars and shipped by overnight courier in coolers with ice packs or on dry ice if the sediment was frozen prior to shipping.

Frozen tissue samples were delivered by EA personnel to Battelle, Norwell, MA, following chain-of-custody procedures. Samples were placed in glass jars, put into coolers and delivered in a refrigerated vehicle.

D. Physical Analysis and Total Organic Carbon

Sub-samples of all sediments were analyzed by ASI personnel for physical parameters. These sediments include the following: each homogenized core, the composite sample, the Mud Dump Reference sediment, and the three Control sediments. Percent moisture and grain size distribution analyses were performed in accordance with the Standard Test Method for Particle-Size Analysis of Soils, Designation: D422-63, Re-approved 2002 [ASTM, 2002].

Total Organic Carbon (TOC) was also determined at ASI based on the guidance from EPA Office of Solids Waste and Emergency Response SW-846 Method No. 9060 (Volume IC, Chapter 5, Revision 0, 9/86). The instrument for this analysis was the Teledyne Tekmar LSS Boat serial number (US18208024R) connected to Teledyne Tekmar Lotix Analyzer serial number (US18214013R).

Additionally, ASI analyzed both composite samples for Bulk Density [Blake, 1986], Specific Gravity [APHA, 1995] and Atterberg Limits (plastic and liquid limits) [ASTM, 1995]. Results of the percent moisture, grain size distribution and TOC analyses are provided in Tables 4a and 4b. Results of the bulk density, specific gravity, and Atterberg limits are provided in Table 5.

E. Solid Phase Testing

Ampelisca abdita, Americamysis bahia

Whole sediment toxicity of the composites was assessed through 10-day exposures with the amphipod, A. abdita, and Mysid shrimp, A. bahia, in solid phase bioassays [ASTM, 1999].

The initial pore water ammonia readings for the composite was 93.9 mg/L, which exceeds the 20.0 mg/L threshold established by Davies et al, [Davies, 1993]. Due to this, pretest purging of the sediment for both of the solid phase studies (A. abdita and A. bahia) was initiated as per the ammonia reduction procedure outlined in the RTM (USACE/USEPA 2016), Section 6.4.5.

At test initiation (Day 0) the ammonia level was measured to be below the required 20.0 mg/L threshold, 13.6 mg/L.

The 10-day studies were conducted as static non-renewal and followed the recommended procedures outlined in the RTM (USACE/USEPA 2016).

Final live count and water quality parameter tables can be found at the end of this report in Tables 7-19.

Standard reference toxicant tests were performed for both organisms, A. bahia and A. abdita. The LC50 values fell within the 95% confidence limit of their respective control charts. The control charts can be found in Volume III, Appendix I.

The A. abdita used in testing were 2-4mm subadults and were obtained from Aquatic Research Organisms, Hampton, NH. Organisms were held for less than 24 hours prior to testing.

The A. bahia used in testing were 3-day-old juveniles and were also obtained from Aquatic Research Organisms, Hampton, NH.

During the holding period both organisms were gradually acclimated to test temperature and salinity.

Organism receiving and holding forms can be found in Volume III, Appendix I.

F. Suspended Particulate Phase Testing

Menidia beryllina, Americamysis bahia and Mytilus galloprovincialis

Toxicity of elutriates prepared from the composite was assessed through 48-and 96-hour suspended particulate phase toxicity tests using three test species, the inland silverside, M. beryllina, the Mysid shrimp, A. bahia, and the mussel, M. galloprovincialis.

The M. beryllina used for testing were 11 days old and were obtained from Aquatic Bio Systems, Inc., Fort Collins, CO.

The A. bahia used for testing were 4-day-old juveniles and were obtained from Aquatic Bio Systems, Inc., Fort Collins, CO.

The M. galloprovincialis used for testing were fertile adults of various ages and were obtained from Aquatic Research Organisms, Hampton, NH.

Organism receiving and holding forms can be found in Volume III, Appendix J.

After 96 hours of exposure to elutriate concentrates, final live count data were transferred into a spreadsheet, sorted and tabulated for M. beryllina and A. bahia.

After 48 hours of exposure to the elutriate concentrations, an EC50 for development and an LC50 for survival were calculated using the ToxCalc statistical software package (Version 5.0, Tidepool Scientific Software) when one or more concentrations showed at least a 50% effect on the M.

galloprovincialis larvae.

Standard reference toxicant tests were performed for M. beryllina, A. bahia, and M. galloprovincialis as cited in the work plan. Reference toxicant data were entered into the ToxCalc statistical software package (Version 5.0, Tidepool Scientific Software) and an LC50 was calculated. The LC50 for all three species fell within the 95% confidence limits of their respective control charts.

Control charts for each species as well as all supporting bioassay documentation can be found in Volume III, Appendix J.

G. Bioaccumulation Testing

Tapes japonica, Alitta virens

The bioaccumulation of metals, pesticides, PCBs, semivolatiles (1-4 dichlorobenzene), PAHs and dioxins/ furans from the composite was assessed using 28-day exposures of the clam, T. japonica and the sand worm, A.

virens.

It should be noted that due to a supplier shortage of the Macoma nasuta, the alternate clam species, T. japonica, was approved to be used for testing by Jim Ferretti of USEPA, Region 2.

The Bioaccumulation studies were conducted following the recommended procedures outlined in the RTM (USACE/USEPA 2016).

The T. japonica used in testing were adults of various ages and were obtained from Aquatic Research Organisms, Hampton, NH.

The A. virens used for testing were adults of various sizes and were obtained from Aquatic Research Organisms, Hampton, NH.

Organism receiving and holding forms can be found in Volume III, Appendix K.

After 28-days of exposure to the composites, organisms were depurated overnight in clean artificial seawater. Tissue from each exposure chamber was placed in individual glass jars, weighed, and frozen before delivering to Battelle by EA personnel for chemical analysis. Tissue samples were archived by Battelle.

Standard reference toxicant tests were performed for both species as cited in the work plan. Water quality and physical parameters were also monitored as per the work plan. The LC50 values for both species fell within the 95% confidence limits of their respective control charts. A control chart for each species, as well as all supporting bioassay documentation data can be found in Volume III, Appendix K.

IV. RESULTS AND DISCUSSION

Test results of all the bioassays, as well as water quality parameters, are summarized in the tables at the end of this volume. Raw data for physical characteristics, biological effects, and water quality parameters, are presented in Volume III.

A. Solid Phase Testing

Ampelisca abdita

After 10 days, survival of A. abdita organisms exposed to the composite was as follows:

A. abdita Survival

Control Reference Composite*

94% 95% 91%

* Statistically significant as compared to the Reference

In accordance with the 1991 Federal Guidance [USEPA, 1991], samples are considered toxic if survival of animals exposed to test sediment are at least 20% less than the survival observed in the reference sediment and this difference is statistically significant (p = 0.05) after a ten-day test period.

These results indicate that the composite is not acutely toxic to A. abdita.

Americamysis bahia

After 10 days, survival of A. bahia organisms exposed to the composite was as follows:

A. bahia Survival

Control Reference Composite

98% 99% 98%

In accordance with the 1991 Federal Guidance [USEPA, 1991], samples are considered toxic if survival of animals exposed to test sediment are at least 10% less than the survival observed in the reference sediment and this difference is statistically significant (p = 0.05) after a ten-day test period.

These results indicate that the composite is not acutely toxic to A. bahia.

B. Suspended Particulate Phase Testing

Menidia beryllina

After 96 hours, M. beryllina exposed to the suspended particulate phase elutriate resulted in an LC50 value of >100% for the composite.

M. beryllina Survival

Concentration Composite

Control 98% 1% 90% 10% 94% 50% 91% 100% 95%

Americamysis bahia

After 96 hours, A. bahia exposed to the suspended particulate phase elutriate resulted in an LC50 value of >100% for the composite.

A. bahia Survival

Control 99% 1% 96% 10% 96% 50% 97% 100% 99%

Mytilus galloprovincialis

After 48 hours, M. galloprovincialis larvae exposed to the suspended particulate phase elutriate resulted in an EC50 value of 80.5% for development and an LC50 value for survival of >100% for the composite.

M. galloprovincialis Survival

Control 92% 1% 91% 10% 92% 50% 92% 100% 70%

Control survival was greater than 90% for M. beryllina and A. bahia. The number of M. galloprovincialis control embryos that resulted in live larvae at the end of the test was greater than 70%. Therefore, the SPP tests passed the criteria for control survival.

C. Bioaccumulation Testing

Tapes japonica

After 28 days, T. japonica exposed to the composite resulted in the following survival:

T. japonica Survival

100% 99% 99%

Alitta (formerly Nereis) virens

After 28 days, A. virens exposed to the composite resulted in the following survival:

A. virens Survival

98% 96% 97%

V. REFERENCES

1. USEPA/USACE, 1991. Evaluation of Dredged Material Proposed for Ocean Disposal, USEPA/ACOE, Washington, DC. EPA-503/8-91/001.

2. USEPA/NYACE, 1992. Draft Guidance for Performing Tests on Dredged Material Proposed for Ocean Disposal. EPA Region II/ACOE New York District (Water Quality Compliance Section), New York, NY.

3. USEPA/NYACE, 2016. Final Guidance for Performing Tests on Dredged Material Proposed for Ocean Disposal. EPA Region II/ACOE New York District (Water Quality Compliance Section), New York, NY.

4. ASTM D422-63, Standard Test Method for Particle-Size Analysis of Soils, ASTM 1963, (reapproved 2002).

5. EPA Office of Solid Wastes & Emergency Response SW-846 Method No. 9060, Volume IC, Chapter 5, Revision 0, 9/86.

6. Blake G.R. and K. H. Hartge. 1986. Bulk Density. In Klute, A. (ed) 1986. Methods of Soil Analysis Part I. Physical and Mineralogical Methods Bulk Density. 2nd ed.

American Society of Agronomy, pp 363-375.

7. APHA, 1995. Standard Methods for the Examination of Water and Wastewater, APHA/AWWA/WPCF. Method 2710 F. Specific Gravity. pp 2-80.

8. ASTM D4318-00, Standard Test Methods for Liquid Limit, Plastic Limit, and Plasticity Index of Soils, 2000.

9. ASTM, 1999. Standard Guide for Conducting 10-Day Sediment Toxicity Tests with Marine and Estuarine Amphipods, E1367-99. American Society of Testing and Materials, Philadelphia, PA.

10. T. Davies, D. Davis, and J. Elmore to EPA Regional Ocean Dumping Coordinators, EPA Regional Wetland Coordinators, and Corps of Engineers Regulatory and Civil Works Elements, 1993. “Technical Panel Recommendations Concerning Use of Acute Amphipod Tests in Evaluation of Dredged Material”. Draft Letter. [Photocopy]. New York District, Army Corps of Engineers, New York, NY.

11. Southerland, Elizabeth to Mario P. Del Vicario, June 14, 1994. “Recommendations for Conducting Sediment Toxicity Test with Mysidopsis bahia when Ammonia may be Present at Toxic Levels”. [Photocopy]. New York, NY.

Table 1a. Sample Identification and Compositing Scheme

Sample Name ASI ID # Composite

S-1 20230021

Composite

ASI ID # 20230048/

EA ID # AT3-072

S-2 20230022 S-3 20230023 S-4 20230024 S-5 20230025 S-6 20230026 S-7 20230027 S-8 20230028 S-9 20230029 S-10 20230030 S-11 20230031 S-12 20230032 S-13 20230033 S-14 20230034 S-15 20230035 S-16 20230036 S-17 20230037 S-18 20230038 S-19 20230039 S-20 20230040 S-21 20230041

Site Water ASI ID # 20230042/

EA ID # AT3-074

Table 1b. Sample Identification- Reference and Control Sediments

Sample ASI ID # EA ID #

Mud Dump Reference 20230002 AT3-073

Solid Phase Control 20230435 AT3-175

Worm Control 20230436 AT3-152

Clam Control 20230437 AT3-085

Table 2. DGPS Coordinates

Core Name Northings Eastings

S-1 666011.8 613452.5 S-2 665686.3 613620.5 S-3 665716.9 613866.5 S-4 666046.0 614625.2 S-5 665984.6 614947.0 S-6 666629.5 612720.6 S-7 666244.5 613069.8 S-8 667118.8 612901.0 S-9 667456.6 612215.1 S-10 667762.7 611730.5 S-11 667948.1 611368.4 S-12 667270.8 611791.2 S-13 667495.9 611442.0 S-14 668071.3 610357.7 S-15 668291.1 609963.0 S-16 668552.8 609475.7 S-17 668821.9 609839.9 S-18 668589.4 610234.0 S-19 668259.7 610765.3 S-20 669950.7 607608.3 S-21 669453.7 608083.6 Site Water 667803.0 611105.7

S-9

S-8

S-7

S-6

S-5

S-4

S-3 S-2

S-1

S-21

S-20

S-19

S-18

S-17

S-16

S-15

S-14

S-13

S-12

S-11

S-10

Source: Esri, Maxar, Earthstar Geographics, and the GIS User Community

608000

608000

609000

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Aqua Survey Inc.

469 Point Breeze Rd.

Flemington, NJ 08822

Tel: (908) 788-8700 Fax: (908) 788-9165 www.AquaSurvey.com

© OpenStreetMap (and) contributors, CC-BY-SA µ

ASI Project: 43-011

Coordinate System:

State Plane NAD 83

New Jersey US Survey Foot

USACE-NYD

Port Jersey Channel

Sample Locations Map

0 500 1,000 1,500250

Feet

Figure 1. Sample Locations Map

Table 3. Test Performance Summary (% Survival)

Test Organism Control Reference Composite

Solid Phase A. abdita 94% 95% 91%

A. bahia 98% 99% 98%

Conc. % Composite

SPP M. beryllina 0 98%

1 90%

10 94%

50 91%

100 95%

A. bahia 0 99%

1 96%

10 96%

50 97%

100 99%

M. galloprovincialis 0 92%

1 91%

10 92%

50 92%

100 70%

Control Reference Composite

Bioaccumulation T. japonica 100% 99% 99%

A. virens 98% 96% 97%

Table 4a. Grain Size Distribution, Percent Moisture, and TOC Values of Test Sediments

Sample ID ASI # Total % Gravel

Total % Sand

Total % Silt

Total % Clay

Moisture

TOC

ppm

% TOC

of Dry Weight

S-1 20230021 0.0 9.2 66.0 24.8 58.28 30,590 3.06 S-2 20230022 0.0 8.3 65.1 26.6 58.40 29,027 2.90 S-3 20230023 0.0 9.4 62.2 28.4 60.10 28,833 2.88 S-4 20230024 0.0 36.5 44.4 19.1 46.32 15,390 1.54 S-5 20230025 0.0 22.0 57.2 20.8 46.99 25,220 2.52 S-6 20230026 0.0 10.0 64.0 26.0 58.13 27,756 2.78 S-7 20230027 0.0 4.9 61.1 34.0 57.73 30,147 3.01 S-8 20230028 0.0 7.6 60.2 32.2 58.46 29,605 2.96 S-9 20230029 0.0 4.6 60.6 34.8 57.56 29,560 2.96 S-10 20230030 0.0 5.9 63.1 31.0 61.32 28,699 2.87 S-11 20230031 0.0 4.3 61.3 34.4 57.88 28,644 2.86 S-12 20230032 0.0 6.9 60.9 32.2 63.34 31,735 3.17 S-13 20230033 0.0 4.7 59.7 35.6 63.40 31,872 3.19 S-14 20230034 0.7 15.2 52.5 31.6 52.25 26,185 2.62 S-15 20230035 0.0 4.1 59.8 36.1 59.55 29,307 2.93 S-16 20230036 0.0 6.8 59.0 34.2 59.24 29,986 3.00 S-17 20230037 0.0 7.5 63.1 29.4 55.33 30,596 3.06 S-18 20230038 0.0 6.5 65.5 28.0 56.29 28,198 2.82 S-19 20230039 0.0 7.0 62.6 30.4 55.85 28,776 2.88 S-20 20230040 0.0 15.8 58.4 25.8 57.65 22,826 2.28 S-20 20230040 dup 0.0 14.3 59.6 26.1 57.54 S-20 20230040 trp 0.0 14.9 62.0 23.1 62.88 S-21 20230041 0.0 7.4 63.5 29.1 63.16 28,598 2.86 Composite 20230048 0.0 11.7 59.8 28.5 56.21 27,169 2.72 Composite 20230048 dup 0.0 12.1 58.9 29.0 56.09 Composite 20230048 trp 0.0 11.8 59.0 29.2 62.88

Table 4b. Grain Size Distribution, Percent Moisture, and TOC Values of Reference and Control Sediments

Table 5. Specific Gravity, Bulk Density, and Atterberg Limits

Sample ID ASI # Total % Gravel

Total % Sand

Total % Silt

Total % Clay

Moisture

TOC

ppm

% TOC

of Dry Weight

Mud Dump Reference 20230002 0.0 97.8 2.2 21.48 553 0.06

Mud Dump Reference 20230002 dup 0.0 97.4 0.3 2.3

Mud Dump Reference 20230002 trp 0.0 97.5 0.2 2.3

Solid Phase Control 20230435 0.0 36.8 49.7 13.5 67.99 32,28 8 3.23

Worm Control 20230436 0.2 56.5 30.0 13.3 28.08 7,759 0.78

Clam Control 20230437 2.1 94.2 1.4 2.3 15.73 1,235 0.12

Sample ID ASI # Specific Gravity

Bulk Density (g/mL)

Atterberg Limits (moisture content) wet dry Liquid Limit Plastic Limit

Composite 20230048 2.50 1.36 0.59 89 38

Composite 20230048 dup 2.53 1.37 0.59 88 39

Composite 20230048 trp 2.51 1.38 0.63 87 38

Mean 2.51 1.37 0.60 88 38

Table 6. Sampling Summary and Holding Times

Sample ASI #/ EA # Sampling

Date Dates Used

Holding Time

Mud Dump Reference

20230002/

AT3-073

1/19/23

3/3/2023 A. abdita solid phase 43 days 3/3/2023 A. bahia solid phase 43 days 3/2/2023 T. japonica bioaccumulation 42 days 3/1/2023 A. virens bioaccumulation 41 days

Composite 20230048/

AT3-072 1/17/23

3/3/2023 A. abdita solid phase 45 days 3/3/2023 A. bahia solid phase 45 days 2/14/2023 M. galloprovincialis suspended particulate phase 28 days 2/20/2023 A. bahia suspended particulate phase 34 days 2/20/2023 M. beryllina suspended particulate phase 34 days 3/2/2023 T. japonica bioaccumulation 44 days 3/1/2023 A. virens bioaccumulation 43 days

Site Water

20230042/

AT3-074

2/6/23

2/14/2023 M. galloprovincialis suspended particulate phase 9 days 2/20/2023 A. bahia suspended particulate phase 14 days 2/20/2023 M. beryllina suspended particulate phase 14 days

Table 7 10-Day Solid Phase Bioassay Species: A. abdita

USACE-NYD: Port Jersey Channel Job #: 43-011 Initial Live Count: 20

Final Live Count

A Solid Phase Control 19 B 20230435 18 C 19 D 20 E 18 94%

A Mud Dump Reference 19 B 20230002 19 C 19 D 19 E 19 95%

A Composite 19 B 20230048 18 C 18 D 18 E 18 91%

Replicate Sample Percent Survival

Table 8 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. abdita USACE- NYD: Port Jersey Channel Job # : 43-011 Temperature (° C)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 19.0 19.0

A Mud Dump Reference 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 19.0 19.0

A Composite 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 19.0 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 19.0 19.0

Range 19.0 19.0

Table 9 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. abdita

Salinity (ppt)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 27.1 28.2 29.3 31.8 30.1 31.1 29.2 33.0 31.0 31.5 31.5 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 27.1 33.0

A Mud Dump Reference 28.2 31.5 32.5 33.0 32.2 32.1 29.7 33.0 31.7 33.0 33.0 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 28.2 33.0

A Composite 28.4 29.0 29.2 30.8 29.9 30.2 28.1 30.3 30.3 30.5 29.6 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 28.1 30.8

Range 27.1 33.0

Table 10 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. abdita

Dissolved Oxygen (mg/L)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

A Solid Phase Control 8.20 7.50 7.80 7.50 7.80 7.90 7.70 7.70 7.70 7.50 7.50 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.50 8.20

A Mud Dump Reference 8.20 7.50 7.80 7.50 7.60 7.60 7.60 7.50 7.60 7.70 7.30 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.30 8.20

A Composite 8.10 7.60 8.00 7.70 7.90 8.10 7.80 7.90 7.90 7.60 7.60 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.60 8.10

Range 7.30 8.20

Table 11 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. abdita pH (SU)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 8.10 7.70 7.90 8.20 7.90 7.80 7.90 8.10 8.10 8.30 8.00 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.70 8.30

A Mud Dump Reference 8.10 8.00 8.00 8.20 8.00 7.90 8.10 8.70 8.20 8.30 8.20 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.90 8.70

A Composite 8.10 7.90 8.00 8.20 8.00 7.80 8.00 8.10 8.10 8.30 8.10 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.80 8.30

Range 7.70 8.70

Table 12 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. abdita

Total Ammonia (mg/L)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 2.30 1.6 1.7 1.3 1.3 1.2 1.4 0.8 0.5 0.4 0.6 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 0.40 2.30

A Mud Dump Reference 0.9 0.9 0.9 0.6 0.6 0.6 <0.50 <0.50 <0.50 <0.50 <0.50 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - <0.50 0.90

A Composite 13.6 13.6 11.8 10.6 10.8 10.1 9.4 8.9 6.7 7.1 7.2 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 6.70 13.6

Range <0.50 13.6

Table 13 10-Day Solid Phase Bioassay Species: A. bahia

Initial Live Count: 20

Final Live Count

A Solid Phase Control 19 B 20230435 19 C 20 D 20 E 20 98%

A Mud Dump Reference 20 B 20230002 20 C 20 D 19 E 20 99%

A Composite 20 B 20230048 20 C 19 D 20 E 19 98%

Replicate Sample Percent Survival

Table 14 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. bahia

Temperature (° C)

A Solid Phase Control 19.0 19.0 19.0 19.7 19.0 19.7 19.0 19.0 19.6 19.5 19.7 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 19.0 19.7

A Mud Dump Reference 19.0 19.9 19.9 20.2 19.7 20.2 19.9 19.7 19.9 20.1 20.3 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 19.0 20.3

A Composite 19.0 19.5 19.2 19.8 19.0 20.0 19.0 19.1 19.6 19.8 20.2 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 19.0 20.2

Range 19.0 20.3

Table 15 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. bahia

Salinity (ppt)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 27.0 28.3 28.1 29.4 30.0 28.7 27.0 31.0 30.9 31.1 29.9 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 27.0 31.1

A Mud Dump Reference 28.9 28.9 28.8 30.4 29.6 29.7 27.7 29.6 29.5 29.7 28.5 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 27.7 30.4

A Composite 28.1 29.1 29.1 30.7 30.1 29.8 27.8 29.6 29.5 29.5 28.3 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 27.8 30.7

Range 27.0 31.1

Table 16 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. bahia

A Solid Phase Control 8.00 8.10 7.70 7.70 8.20 7.00 7.70 7.80 7.50 7.60 7.50 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.00 8.20

A Mud Dump Reference 7.80 6.90 7.50 7.30 7.50 6.90 7.50 7.50 7.40 7.50 7.40 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 6.90 7.80

A Composite 8.00 6.80 7.60 7.70 7.80 6.60 7.80 7.70 7.60 7.50 7.40 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 6.60 8.00

Range 6.60 8.20

Table 17 10 Day Solid Phase Readings (Static Non- Renewal) Species: A. bahia pH (SU)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 7.90 7.90 8.00 8.20 7.90 8.00 8.10 8.10 8.10 8.20 8.00 B 20230435 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.90 8.20

A Mud Dump Reference 8.00 8.00 8.10 8.20 7.90 8.00 8.10 8.10 8.10 8.20 8.20 B 20230002 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.90 8.20

A Composite 7.90 8.00 8.10 8.20 7.90 7.90 8.10 8.10 8.10 8.20 8.10 B 20230048 - - - - - - - - - - - C - - - - - - - - - - - D - - - - - - - - - - - E - - - - - - - - - - - 7.90 8.20

Range 7.90 8.20

Table 18 10 Day Solid Phase Readings (Static Non-Renewal) Species: A. bahia USACE-NYD: Port Jersey Channel Job # : 43-011 Overlaying Ammonia (mg/L NH3-N)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 0.80 0.80 0.80 0.70 0.60 0.40 0.20 0.10 0.20 <0.10 <0.10 B 20230435 C D E <0.10 0.80

A Mud Dump Reference 3.00 3.20 <0.10 <0.10 <0.10 <0.10 <0.10 <0.10 <0.10 <0.10 <0.10 B 20230002 C D E <0.10 3.20

A Composite 4.70 4.30 4.20 3.10 2.80 2.00 1.90 1.50 1.40 1.00 0.90 B 20230048 C D E 0.90 4.70

Range <0.20 4.70

Table 19 10 Day Solid Phase Readings (Static Non-Renewal) Species: A. bahia USACE-NYD: Port Jersey Channel Job # : 43-011 Unionized Ammonia (mg/L NH3-N)

Replicate Sample 0 1 2 3 4 5 6 7 8 9 10 Low High A Solid Phase Control 0.02 0.02 0.02 0.03 0.01 0.01 0.01 0.00 0.01 0.00 0.00 B 20230435 C D E 0.00 0.03

A Mud Dump Reference 0.09 0.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 B 20230002 C D E 0.00 0.10

A Composite 0.11 0.13 0.15 0.15 0.06 0.05 0.07 0.05 0.05 0.05 0.03 B 20230048 C D E 0.03 0.15

Range 0.00 0.15

Table 20 96-hour Suspended Particulate Phase Species: M. beryllina

Initial Live Count: 20

Replicate Sample Final Live Count Percent Survival LC50

A Control 20 B 20 C 18 D 20 E 20 98%

A Composite 19

B 1% SPP 17

C 19 D 18 E 17 90%

A Composite 18

B 10% SPP 18

C 19 D 20 E 19 94%

A Composite 14

B 50% SPP 19

C 19 D 20 E 19 91%

A Composite 19

B 100% SPP 20

C 20 D 19 E 17 95% >100%

Table 21 96 Hour Suspended Particulate Phase Test Species: M. beryllina USACE-NYD: Port Jersey Channel Job No. 43-011

Replicate Sample 0 24 48 72 96 Low High

A Control 20.0 19.8 19.5 19.9 19.3 B 19.6 19.5 20.2 19.5 C 19.6 19.5 20.2 19.6 D 19.9 19.5 20.2 19.6 E 19.7 19.5 20.2 19.6 19.3 20.2

A Composite 20.1 20.0 19.5 20.2 19.5

B 1% SPP 19.6 19.5 20.2 19.5

C 19.8 19.5 20.2 19.5 D 19.8 19.5 20.2 19.4 E 19.7 19.4 20.2 19.4 19.4 20.2

A Composite 20.1 19.8 19.3 20.3 19.5

B 10% SPP 19.7 19.3 20.3 19.5

C 19.9 19.3 20.3 19.5 D 19.9 19.4 20.3 19.4 E 19.9 19.4 20.3 19.4 19.3 20.3

A Composite 20.2 19.8 19.4 20.2 19.5

B 50% SPP 19.8 19.4 20.2 19.5

C 19.8 19.4 20.2 19.5 D 19.9 19.4 20.3 19.5 E 19.8 19.4 20.3 19.4 19.4 20.3

A Composite 20.3 19.9 19.4 20.2 19.5

B 100% SPP 19.7 19.4 20.2 19.5

C 19.8 19.4 20.2 19.5 D 19.8 19.5 20.2 19.5 E 19.8 19.4 20.3 19.5 19.4 20.3

Range 19.3 20.3

Table 22 96 Hour Suspended Particulate Phase Test Species: M. beryllina

Salinity (ppt)

A Control 27.2 27.6 27.0 27.6 27.0 B 27.4 27.0 27.3 27.0 C 27.3 27.0 27.3 27.0 D 27.3 27.0 27.4 27.0 E 27.3 27.0 27.4 27.0 27.0 27.6

A Composite 27.1 27.6 27.0 27.5 27.0

B 1% SPP 27.3 27.0 27.3 27.0

C 27.2 27.0 27.4 27.0 D 27.2 27.0 27.4 27.0 E 27.2 27.0 27.4 27.0 27.0 27.6

A Composite 27.3 27.7 27.2 27.7 27.0

B 10% SPP 27.5 27.2 27.6 27.0

C 27.3 27.0 27.4 27.0 D 27.4 27.2 27.5 27.0 E 27.4 27.0 27.5 27.0 27.0 27.7

A Composite 28.0 28.3 27.7 28.2 27.1

B 50% SPP 27.9 27.6 28.1 27.0

C 27.9 27.6 28.1 27.0 D 27.9 27.6 28.0 27.0 E 27.9 27.6 28.1 27.1 27.0 28.3

A Composite 28.7 29.0 28.4 28.8 27.7

B 100% SPP 28.6 28.3 28.7 27.6

C 28.6 28.3 28.7 27.5 D 28.6 28.3 28.7 27.5 E 28.6 28.3 28.8 27.6 27.5 29.0

Range 27.0 29.0

Table 23 96 Hour Suspended Particulate Phase Test Species: M. beryllina

A Control 7.30 6.90 6.70 6.80 6.30 B 7.30 6.70 6.50 5.90 C 7.20 6.70 6.40 5.70 D 7.10 6.50 6.20 5.50 E 7.20 6.50 6.10 5.40 5.40 7.30

A Composite 7.30 6.90 6.70 6.00 5.70

B 1% SPP 7.20 6.70 6.00 6.00

C 7.10 6.80 5.80 6.00 D 7.10 6.80 5.60 5.80 E 7.10 6.70 5.50 5.70 5.50 7.30

A Composite 7.30 6.90 6.70 5.70 5.60

B 10% SPP 7.20 6.70 5.70 5.70

C 7.10 6.90 5.70 5.70 D 7.20 6.70 5.60 5.70 E 7.10 6.50 5.60 5.60 5.60 7.30

A Composite 7.40 6.90 6.60 5.80 6.00

B 50% SPP 7.10 6.60 5.90 6.00

C 7.20 6.80 6.00 5.90 D 7.10 6.80 5.90 5.90 E 7.20 6.80 6.00 6.00 5.80 7.40

A Composite 7.40 6.80 6.70 5.90 5.80

B 100% SPP 7.00 6.70 6.00 5.80

C 6.90 6.60 5.70 5.60 D 6.80 6.60 5.60 5.70 E 6.90 6.60 5.80 5.60 5.60 7.40

Range 5.40 7.40

Table 24 96 Hour Suspended Particulate Phase Test Species: M. beryllina pH

A Control 7.90 8.10 8.10 8.00 8.00 B 7.90 8.10 8.00 8.00 C 7.90 8.10 8.10 8.00 D 8.00 8.10 8.10 8.00 E 8.00 8.10 8.10 8.00 7.90 8.10

A Composite 8.00 8.10 8.10 8.10 8.00

B 1% SPP 8.00 8.10 8.10 8.00

C 8.00 8.10 8.10 8.00 D 8.00 8.10 8.10 8.00 E 7.90 8.10 8.10 8.00 7.90 8.10

A Composite 8.00 8.10 8.10 8.10 8.00

B 10% SPP 8.00 8.10 8.10 8.00

C 8.00 8.10 8.10 8.00 D 8.00 8.10 8.10 8.00 E 8.00 8.10 8.10 8.00 8.00 8.10

A Composite 8.00 8.10 8.10 8.10 8.00

B 50% SPP 8.00 8.10 8.10 8.00

C 8.00 8.10 8.10 8.00 D 8.00 8.10 8.10 8.00 E 8.00 8.10 8.10 8.00 8.00 8.10

A Composite 7.90 8.10 8.10 8.10 8.00

B 100% SPP 8.00 8.10 8.10 8.00

C 8.00 8.10 8.10 8.00 D 8.00 8.10 8.10 8.00 E 8.00 8.10 8.10 8.00 7.90 8.10

Range 7.90 8.10

Table 25 96-hour Suspended Particulate Phase Species: A. bahia

Initial Live Count: 20

Replicate Sample Final Live Count Percent Survival LC50

A Control 20 B 20 C 19 D 20 E 20 99%

A Reach 1 Composite 20

B 1% SPP 19

C 20 D 18 E 19 96%

A Reach 1 Composite 19

B 10% SPP 20

C 19 D 19 E 19 96%

A Reach 1 Composite 20

B 50% SPP 18

C 19 D 20 E 20 97%

A Reach 1 Composite 20

B 100% SPP 20

C 19 D 20 E 20 99% >100%

Table 26 96 Hour Suspended Particulate Phase Test Species: A. bahia

A Control 20.0 19.1 19.3 19.9 19.5 B 19.4 19.3 20.1 19.5 C 19.5 19.4 20.2 19.5 D 19.7 19.4 20.2 19.5 E 19.7 19.4 20.2 19.5 19.1 20.2

A Composite 20.1 19.5 19.3 20.1 19.4

B 1% SPP 19.7 19.4 20.2 19.4

C 19.8 19.4 20.2 19.4 D 19.9 19.4 20.2 19.4 E 19.9 19.4 20.1 19.4 19.3 20.2

A Composite 20.1 19.8 19.4 20.1 19.4

B 10% SPP 19.8 19.4 20.2 19.4

C 19.8 19.5 20.2 19.4 D 19.8 19.5 20.2 19.4 E 19.8 19.5 20.2 19.3 19.3 20.2

A Composite 20.2 19.8 19.4 19.8 19.4

B 50% SPP 19.8 19.5 20.1 19.4

C 19.5 19.5 20.2 19.4 D 19.9 19.5 20.2 19.5 E 19.9 19.5 20.2 19.4 19.4 20.2

A Composite 20.3 19.9 19.5 20.1 19.4

B 100% SPP 19.8 19.5 20.2 19.5

C 19.8 19.4 20.2 19.5 D 19.7 19.4 20.2 19.5 E 19.7 19.4 20.2 19.4 19.4 20.3

Range 19.1 20.3

Table 27 96 Hour Suspended Particulate Phase Test Species: A. bahia

A Control 27.2 27.9 27.1 27.7 27.0 B 27.3 27.1 27.5 27.0 C 27.2 27.1 27.3 27.0 D 27.2 27.0 27.4 27.0 E 27.2 27.0 27.4 27.0 27.0 27.9

A Composite 27.1 27.8 27.1 27.5 28.5

B 1% SPP 27.2 27.0 27.3 28.0

C 27.2 27.0 27.3 27.9 D 27.2 27.0 27.3 27.9 E 27.2 27.0 27.3 27.9 27.0 28.5

A Composite 27.3 27.8 27.2 27.6 27.0

B 10% SPP 27.3 27.0 27.4 27.0

C 27.3 27.0 27.4 27.0 D 27.4 27.0 27.5 27.0 E 27.3 27.1 27.6 27.0 27.0 27.8

A Composite 28.0 28.3 27.6 28.2 27.1

B 50% SPP 27.8 27.5 28.0 27.0

C 27.9 27.5 28.0 27.0 D 27.9 27.5 28.0 27.0 E 27.9 27.5 28.0 27.0 27.0 28.3

A Composite 28.7 29.0 28.4 29.0 27.8

B 100% SPP 28.6 28.3 28.8 27.7

C 28.6 28.3 28.8 27.6 D 28.6 28.3 28.8 27.8 E 28.7 28.4 28.8 27.9 27.6 29.0

Range 27.0 29.0

Table 28 96 Hour Suspended Particulate Phase Test Species: A. bahia

Dissolved Oxygen (mg/L)

Replicate Sample 0 24 48 72 96 Low High A Control 7.30 7.30 6.80 6.60 6.30 B 7.10 6.70 6.10 5.90 C 6.80 6.60 5.60 5.50 D 6.70 6.50 5.50 5.50 E 6.40 6.40 5.00 5.40 5.00 7.30

A Composite 7.30 7.00 6.60 4.80 5.40

B 1% SPP 6.40 6.70 4.90 5.20

C 6.50 6.70 4.50 5.00 D 6.50 6.50 4.60 5.40 E 6.20 6.40 4.90 5.40 4.50 7.30

A Composite 7.30 7.00 6.60 4.70 5.10

B 10% SPP 6.50 6.60 4.60 4.90

C 6.80 6.70 4.40 5.00 D 6.80 6.60 4.60 5.10 E 6.90 6.70 4.90 5.20 4.40 7.30

A Composite 7.40 6.70 6.80 7.00 5.20

B 50% SPP 6.20 6.40 6.50 5.00

C 6.20 6.30 5.50 4.80 D 6.00 6.20 4.80 5.10 E 6.10 6.20 4.40 5.00 4.40 7.40

A Composite 7.40 6.50 6.40 4.60 5.10

B 100% SPP 6.50 6.50 4.70 4.90

C 6.50 6.40 4.60 4.70 D 6.50 6.50 4.70 4.80 E 6.40 6.30 4.60 5.00 4.60 7.40

Range 4.40 7.40

Table 29 96 Hour Suspended Particulate Phase Test Species: A. bahia

A Control 7.90 8.10 7.90 7.90 7.90 B 8.00 7.90 8.00 7.90 C 7.90 7.90 8.00 7.90 D 7.90 7.90 8.00 7.90 E 7.90 7.90 8.00 7.90 7.90 8.10

A Composite 8.00 8.10 7.90 8.00 7.90

B 1% SPP 8.00 7.90 8.00 7.90

C 8.00 7.90 8.00 7.90 D 8.00 7.90 8.00 7.90 E 8.00 8.00 8.00 7.90 7.90 8.10

A Composite 8.00 8.10 8.00 8.00 7.90

B 10% SPP 8.00 8.00 8.00 7.90

C 7.90 8.00 8.00 7.90 D 7.90 8.00 8.00 7.90 E 7.90 8.00 8.00 7.90 7.90 8.10

A Composite 8.00 8.10 8.00 8.00 7.90

B 50% SPP 7.90 8.00 8.00 7.90

C 7.90 8.00 8.00 7.90 D 7.90 8.00 8.00 7.90 E 7.90 8.00 8.00 7.90 7.90 8.10

A Composite 7.90 8.10 8.00 8.00 7.90

B 100% SPP 7.90 8.00 8.00 7.90

C 7.90 8.00 8.00 7.90 D 7.90 8.00 8.00 7.90 E 7.90 8.00 8.00 7.90 7.90 8.10

Range 7.90 8.10

Table 30 Summary Data Sheet for M. galloprovincialis Embryo Development Test-Proportion Alive ASI Job No. 43-011 USACE-NYD: Port Jersey Channel

LC50 Composite- 20230048 2/15-17/23 48 Hours

Concentration Replicate Proportion N-CDS D-CDS LL-CDS B Initial A M/"M" % E Control Survival Counted (N-CDS +D-CDS) Population 100(A-M)/(100-M) (N-CDS + D-CDS + LL-CDS)

0 A 1/20 197 15 0 4240 4480 5.36 4240 B 1/20 188 17 0 4100 4480 8.48 4100 C 1/20 199 13 0 4240 4480 5.36 4240 D 1/20 184 12 0 3920 4480 12.50 3920 E 1/20 189 14 0 4060 4480 9.38 4060

Mean % 8.21 91.79 91.79

1% SPP A 1/20 182 16 0 3960 4480 11.61 3.70

B 1/20 179 13 0 3840 4480 14.29 6.61 C 1/20 196 15 0 4220 4480 5.80 -2.63 D 1/20 208 12 0 4400 4480 1.79 -7.00 E 1/20 185 17 0 4040 4480 9.82 1.75

Mean % 8.66 0.49

91.34 99.51

10% SPP A 1/20 186 24 0 4200 4480 6.25 -2.14

B 1/20 189 25 0 4280 4480 4.46 -4.09 C 1/20 176 26 0 4040 4480 9.82 1.75 D 1/20 183 19 0 4040 4480 9.82 1.75 E 1/20 181 22 0 4060 4480 9.38 1.26

Mean % 7.59 -0.29

92.41 100.29

50% SPP A 1/20 179 27 0 4120 4480 8.04 -0.19

B 1/20 171 33 0 4080 4480 8.93 0.78 C 1/20 192 28 0 4400 4480 1.79 -7.00 D 1/20 180 29 0 4180 4480 6.70 -1.65 E 1/20 169 21 0 3800 4480 15.18 7.59

Mean % 8.13 -0.10

91.88 100.10

100% SPP A 1/20 42 117 0 3180 4480 29.02 22.67

B 1/20 47 105 0 3040 4480 32.14 26.07 C 1/20 32 111 0 2860 4480 36.16 30.45 D 1/20 50 123 0 3460 4480 22.77 15.86 E 1/20 45 115 0 3200 4480 28.57 22.18

Mean % 29.73 23.44

70.27 76.56

LC50 = >100%

N-CDS= Number of larvae with completely developed shells D-CDS= Number of deformed larvae with completely developed shells LL-CDS= Number of live larvae with earlier stage than completely developed shells stage A= The % of embryos that did not result in live larvae A = 100 (N-B)/N B= The number of live larvae at the end of the test N= The number of embryos in the 2-cell stage or beyond at the beginning of the test (initial count) M= Average % of the embryos that did not result in live larvae with completely developed shells in the control treatments The test is unacceptable if M is >30% for oysters or >40% for hard clams E = Abbott's formula = 100(A-M)/(100-M) E = The % of introduced live embryos that did not result in live larvae with completely developed shells adjusted for the control

Table 31 Summary Data Sheet for M. galloprovincialis Embryo Development Test - Proportion Normal ASI Job No. 43-011 USACE-NYD: Port Jersey Channel

EC50 Composite- 20230048 2/15-17/23 48 Hours

Concentration Replicate Proportion N-CDS D-CDS LL-CDS B Initial A M/"M" % E Control Survival Counted (N-CDS) Population 100(A-M)/(100-M) (N-CDS + D-CDS + LL-CDS)

0 A 1/20 197 15 0 3940 4480 12.05 4240 B 1/20 188 17 0 3760 4480 16.07 4100 C 1/20 199 13 0 3980 4480 11.16 4240 D 1/20 184 12 0 3680 4480 17.86 3920 E 1/20 189 14 0 3780 4480 15.63 4060

Mean % 14.55 91.79 85.45

1% SPP A 1/20 182 16 0 3640 4480 18.75 4.91

B 1/20 179 13 0 3580 4480 20.09 6.48 C 1/20 196 15 0 3920 4480 12.50 -2.40 D 1/20 208 12 0 4160 4480 7.14 -8.67 E 1/20 185 17 0 3700 4480 17.41 3.34

Mean % 15.18 0.73

84.82 99.27

10% SPP A 1/20 186 24 0 3720 4480 16.96 2.82

B 1/20 189 25 0 3780 4480 15.63 1.25 C 1/20 176 26 0 3520 4480 21.43 8.05 D 1/20 183 19 0 3660 4480 18.30 4.39 E 1/20 181 22 0 3620 4480 19.20 5.43

Mean % 18.30 4.39

81.70 95.61

50% SPP A 1/20 179 27 0 3580 4480 20.09 6.48

B 1/20 171 33 0 3420 4480 23.66 10.66 C 1/20 192 28 0 3840 4480 14.29 -0.31 D 1/20 180 29 0 3600 4480 19.64 5.96 E 1/20 169 21 0 3380 4480 24.55 11.70

Mean % 20.45 6.90

79.55 93.10

100% SPP A 1/20 42 117 0 840 4480 81.25 78.06

B 1/20 47 105 0 940 4480 79.02 75.44 C 1/20 32 111 0 640 4480 85.71 83.28 D 1/20 50 123 0 1000 4480 77.68 73.88 E 1/20 45 115 0 900 4480 79.91 76.49

Mean % 80.71 77.43

19.29 22.57

EC50 = 80.5%

N-CDS= Number of larvae with completely developed shells D-CDS= Number of deformed larvae with completely developed shells LL-CDS= Number of live larvae with earlier stage than completely developed shells stage A= The % of embryos that did not result in live non-deformed larvae A = 100 (N-B)/N B= The number of live non-deformed larvae at the end of the test N= The number of embryos in the 2-cell stage or beyond at the beginning of the test (initial count) M= Average % of the embryos that did not result in live non-deformed larvae with completely developed shells in the control treatments E = Abbott's formula = 100(A-M)/(100-M) E = The % of introduced live embryos that did not result in live non-deformed larvae with completely developed shells adjusted for the control

Table 32 48 Hour Suspended Particulate Phase Test Species: M. galloprovincialis

Temperature…