Arecibo_December_2017_mold_report_(002).pdf

ALTOL ENVIRONMENTAL SERVICES INC.

ENVIRONMENTAL SURVEY FOR MOLD AIR MONITORING

JOB CORPS ARECIBO DECEMBER 11, 2017

ALTOL ENVIRONMENTAL SERVICES, INC.

Ponce, Puerto Rico

Indoor Air Monitoring Job Corps Arecibo December 11, 2017

Prepared by: Margarita Irizarry Cardona MES. Air Monitoring Specialist (Lic. ASB-0912-0557-MS), Supervisor, LBP Inspector, Asbestos Inspector

TABLE OF CONTENTS

Statement of Limitation……………………………………………………………….3

Executive Summary……………………………………………………..4

Table 1.0 Location and Description of Sample

Project Location and Background……………………………………………………5

Analysis Results………………………………………………………………………..5

Table 2.0 Total Concentration of Mold Spores in Air Samples

Table 2.1 Total Concentration of Mold Types in Air Samples

Table 3.0 Concentration of spore’s type in different sampling areas

Table 3.1 Concentration of mold Types in Air Samples

Figure 1.0 Concentration of Spores Types in Air Samples

Characteristic of Mold Growth and Behavior……………………………………….8

Remediation Recommendation……………………………………………………….9

Appendix A: Site Location………………………………………………………………10

Appendix B: Equipment Specification…………………………………………………13

Appendix C: Environmental Microbiology Laboratory Test Results………………….15

Appendix D: Mold Inspection and Testing Photos…………………………………….16

STATEMENT OF LIMITATION

The following mold assessment is based on findings of the physical inspection and testing. Findings are current and accurate for the date and time they were found, but do not reflect expected or predictable mold growth and infestation on and within the property. This report addresses only those areas physically inspected and sampled. The Inspector is not responsible or liable for the non-discovery of any water damage, water problems, mold contamination, or other conditions of the subject facility which may occur or may become evident after the inspection and testing time and date.

Executive Summary

Mold assessment and testing protocol has been performed by ALTOL ENVIROMENTAL personnel on November 27, 2017. The study was conducted at the Job Corps facilities, located in Garrochales, Arecibo P.R. The results of the sampling event are presented herein.

Sampling protocol for biological contaminants is based on bioaerosol sampling (Indoor Air), spore traps and Andersen technique. The purpose of these methods was the identification of culturable microorganism and assessment of possible proliferation and dissemination of fungi in the different buildings of the facility. The samples were taken as requested. Ten (10) areas were identified to sampled and compared with the outside environmental conditions.

A list of the equipment used in the project is attached in Appendix B. Calibration sheets of pump rotameters are also included. Eleven (11) spore trap and viable mold samples were collected at the subject facility. The sampling pump was calibrated to 15 L/min on site before samples were collected. The sampling cassettes used for the testing were Allergenco D. The Allergenco D sampling cassette were developed for sampling and collection of aeroallergen and bioaerosols for quantitative analysis of mold spore. The microbiological air quality was evaluating by using a one stage air sampler, based on The Andersen Principles (Impactor sampling) All samples were delivered to the Environmental Microbiology Laboratory for analysis. The Table 1.0 provides descriptions of each sample, location, relative humidity and structural observations.

Table 1.0 Location and Description

Sample Location RH

Structural Observation

Health & Wellness Dpt. Bldg. #1 66 A small area in ceiling tile with mold, no structural damage, leaks or water intrusion were observed

Retail Sales Dpt. 63 Structural damage on roof, ceiling tiles, dampness on building.

No visible mold and no odor were present

Administration Dpt. 61 Structural damage on roof and water leak.

Visible mold on floor, no odor was present.

Sport Pavillion Bldg #9 61 No structural damage, some ceiling tiles with damage.

Visible mold on chairs and pool table

Outside Area 58 No observations

CTT Manager Office 63 No structural damage. Visible mold on drywalls (lower area)

Office Administration Classroom 62 Several structural damages on roof, dampness in the ceiling tiles.

Male Residence- RA Office B Bldg. #18 64 No structural damage or dampness. Visible mold was found.

Residential Life Management Office Bldg #27 66 Several Structural damages on roof

Female Residence Lobby 61 No structural damage

Licensed Practical Nurse Classroom 72 Several structural damages on roof, dampness and odor.

PROJECT LOCATION AND BACKGROUND

A sampling event to assess microbiological presence was conducted by ALTOL ENVIROMENTAL personnel on November 27, 2017. The study was conducted at Job Corps, located at

Garrochales, Arecibo, P.R. The assessment was requested due to concerns of moisture intrusion and mold growth in the facility. These areas were impact after the passing of the hurricane Maria and physical evidence was observed of mold growth. Physical evidence was portrayed by photos of each area of how they were found before the assessment (Appendix D).

ANALYTICAL RESULTS

The results of the sampling event are shown in Appendix IV. The Chain of Custody is also included

(Refer to Appendix C). Samples collected were analyzed by CEI Laboratory, an AIHA-LAP, LLC-

EMLAP lab 103025 accredited laboratory. Air samples were taken to establish the concentration of mold and spores in ambient air. Under normal conditions, the concentration of mold inside should be approximately equivalent to or lower than the concentration of spore captured outside of the impact area. The summary of total concentration of mold spore per cubic meter of air from each sample is given in Table 2.0. The Table 2.1 show the total of culturable mold results.

Table 2.0 Total Concentration of Mold Spore in Air Sample (Spore Trap Samples) Sample ID Sample Description Concentration (spore/m3)

3157-112717-ST-01 Health & Wellness Dpt. Bldg. #1 7700

3157-112717-ST-02 Retail Sales Dpt. 6000

3157-112717-ST-03 Administration Dpt. 1900

3157-112717-ST-04 Sport Pavillion Bldg #9 6000

3157-112717-ST-05 Outside Area 1700

3157-112717-ST-06 CTT Manager Office 4100

3157-112717-ST-07 Office Administration Classroom 12000

3157-112717-ST-08 Male Residence- RA Office B Bldg. #18 3400

3157-112717-ST-09 Residential Life Management Office Bldg #27 3200

3157-112717-ST-10 Female Residence Lobby 4800

3157-112717-ST-11 Licensed Practical Nurse Classroom 9300

Table 2.1 Total Concentration of Mold Spore in Air Sample (Culturable Samples) Sample ID Sample Description Concentration

(spore/m3)

3157-112717-75-MI-01 Health & Wellness Dpt. Bldg. #1 610

3157-112717-75-MI-02 Retail Sales Dpt. 1200

3157-112717-75-MI-03 Administration Dpt. 410

3157-112717-75-MI-04 Sport Pavillion Bldg #9 450

3157-112717-75-MI-05 Outside Area 520

3157-112717-75-MI-06 CTT Manager Office 990

3157-112717-75-MI-07 Office Administration Classroom 1100

3157-112717-75-MI-08 Male Residence- RA Office B Bldg. #18 770

3157-112717-75-MI-09 Residential Life Management Office Bldg #27 530

3157-112717-75-MI-10 Female Residence Lobby 990

3157-112717-75-MI-11 Licensed Practical Nurse Classroom 1000

Mold are common in buildings and homes and will grow anywhere indoors where there is moisture. The common indoor molds are Cladosporium, Aspergillus, Penicillium, and

Alternaria. The concentration of individual spore’s types from each sample is given in Table 3.0 and mold type in Table 3.1 In order to determine areas of elevated mold growth the concentration of individual mold types in each sample can be directly compared to the concentration of the same mold type in outside area. The actual value of Aspergillus/

Penicillinum captured in Health department, Retail Sales Department, Sport Pavillion, CTT

Manager Office, Office Administration Classroom, Male residences, Female residences and

Licensed Practical Nurse Classroom samples exceed this level, compared to the outside area sample, therefore the value is provided on the Table 3.0. The Cladosporium also exceed levels in Health department, Retail Sales Department, Office Administration Classroom, Male

Residences, Female Residences and Licensed Practical Nurse Classroom compared with the outside area. Ascospore was found in higher level in CTT Manager Office than Outside Area.

There was no spores or growth of Strachybotrys in this inspection. Active growth in the CTT

Manager, Office Administration Classroom, Female Residence, and Licensed Practical Nurse

Classroom has been confirm by the concentrations of Cladosporium. The figure 1.0 and 1.1 shows the spores concentration and mold growth concentration by locations.

Table 3.0 Concentration of Mold Type Compared with the Different Sampling Areas Location Area Ascospore

Spores/m3

Basidiospore

Spores/m3

Culvaria

Spores/m3

Cercospore

Spores/m3

Periconia

Spores/m3

Fusarium

Spores/m3

Aspergillus/

Penicillium

Spores/m3

Cladosporium

Spores/m3

Health & Wellness Dpt. Bldg. #1 707 440 40 13 40 67 4926 1360

Retail Sales Dpt. 627 147 147 67 27 -- 3547 2040

Administration Dpt. 613 200 107 27 93 93 200 413

Sport Pavillion Bldg #9 387 107 27 -- 13 53 4782 333

Outside Area 600 227 13 -- 280 27 107 320

CTT Manager Office 1040 427 160 53 120 27 1213 973

Office Administration Classroom 213 307 573 93 360 107 8598 1080

Male Residence- RA Office B Bldg.

#18

333 40 120 147 13 160 1333 1187

Residential Life Management Office Bldg #27

507 93 1373 67 93 13 27 867

Female Residence Lobby 173 53 507 93 13 133 947 2287

Licensed Practical Nurse Classroom 93 80 27 27 27 80 6984 1867

Table 3.1 Summary of mold growth concentration by types

Location Cladosporium CFU/m3

Aspergillus niger CFU/m3

Penicillium CFU/m3

Sterilia mycelia CFU/m3

Fusarium CFU/m3

Health & Wellness Dpt. Bldg. #1 547 -- -- 67 -- Retail Sales Dpt. 88 -- -- 67 --

Administration Dpt. 227 53 67 53 -- Sport Pavillion Bldg #9 240 27 107 80 --

Outside Area 333 13 53 93 27 CTT Manager Office 893 -- -- 80 --

Office Administration Classroom 960 -- 13 107 -- Male Residence- RA Office B Bldg. #18 573 40 27 133 --

Residential Life Management Office Bldg #27 467 -- -- 67 -- Female Residence Lobby 840 -- -- 147 --

Licensed Practical Nurse Classroom 720 -- 40 187 67

The Appendix B provides a Mold report prepared by CEI Labs, an AIHA LAP, LLC accredited laboratory with id #1033025. This method of analysis used for the spore samples collected at the subject facility was nonviable direct microscopy. The method used for the mold growth concentration was the Culturable Method to determine the concentration of mold types in samples.

CHARACTERISTIC OF MOLD GROWTH AND BEHAVIOR

Mold spores have been shown to produce allergic reactions in people susceptible to them. Spores may affect the respiratory system, producing sinusitis, Type I allergies (hay fever, asthma) and/or

Type III hypersensitivity pneumonitis. Topical reactions may include rashes, irritation, and dermatitis. Species associated with the production of mycotoxins are specifically noted bellow.

Although most species of mold require high moisture levels for active growth, the reduction of moisture level will not kill mold. Mold species will go dormant at low moisture levels. There are some species that can continue active growth and spore production even at low moisture levels, particularly certain species of Aspergillus. Following is a description of mold types captured at the subject facility.

Basidiospores are group of spores produce by over 1200 different types of fungi, including many mushrooms. They are common in cosmopolitan areas associated with gardens and forest. Indoor environment, Basidospore are typically found on wood or cellulitic material.

Cladosporium is common in indoor environments. This group molds is the most common worldwide. In outdoor environments, Cladosporium is associated with many types of soil, plant litter, and plant pathogens. In indoor environments, Cladosporium is typically found on moist windowsills, textiles, and wood.

Penicillinum and Aspergillus are included as one group due to to similarities in the appearance of their spore and growth patterns. They are often associated with house dust, but, may also be found growing on cellulitic materials, such as sheet rock, and wood. This group of molds is often the first to colonize an area and is marked by rapid spore production and colony growth.

Ascospore grows well under a variety of conditions and many are known to be plant pathogens.

Ascospores are microscopic spores which develop during the winter on dead, fallen leaves that were infected the previous season.

Stachybotrys chartarum is found on very moist cellulitic materials, such as gypsum board, paint, wallboard, etc. This species requires prolonged exposure to elevated moisture levels for growth.

Although it is typically not the first species to colonize an area and does not grow as rapidly as molds such as Penicillium, Stachybotrys chartarum may become dominant given enough time.

This species is associated with the production of mycotoxins that are potentially harmful to human health. Stachybotrys chartarum was captured in the wall of the northwest bedroom closet, northwest bathroom, and ceiling of the southwest bedroom.

There is evidence of exposure to dampness/ mold and health effects. Recently a published document by the World Health Organization (a book titled, WHO Guidelines for Indoor Air

Quality: Dampness and Mold – September 2009), has stated:

1. The overall evidence shows that house dampness is consistently associated with a wide range of respiratory health effects, most notably asthma, wheeze, cough, respiratory infections and upper respiratory tract symptoms. However, there is insufficient evidence of a causal relationship with any of the health outcomes reviewed.

2. An association between the presence of “mold” and bacteria in damp indoor environments and hypersensitivity pneumonitis has been reported repeatedly.

3. A study by Fisk, Lei-Gomez and Mendell (2007) concluded that “building dampness and mould are associated with approximately 30-50% increases in a variety of respiratory and asthma-related health outcomes.

Conclusion and Remediation Recommendation

Having completed the visual assessment and sampling procedures, it can be concluded that presence of molds was found at the moment of the inspection. The actual spores value of

Aspergillus/ Penicillinum captured in Health department, Retail Sales Department, Sport

Pavillion, CTT Manager Office, Office Administration Classroom, Male residences, Female residences and Licensed Practical Nurse Classroom exceed the level, compared to the outside area sample. The Cladosporium also exceed levels in Health department, Retail Sales

Department, Office Administration Classroom, Male Residences, Female Residences and

Licensed Practical Nurse Classroom compared with the outside area.

Active mold growth was confirmed in the CTT Manager, Office Administration Classroom, Female Residence, and Licensed Practical Nurse Classroom for Cladosporium.

In isolated areas and those areas less than 10 ft, remediation can be handled by regular maintenance personnel. Areas of mold infestation over 10 ft2 should be remediated by a professional remediation company with training and experience dealing with mold. Proper remediation protocol may include the following, though additional measures may be necessary and will be determined by the remediation company chosen.

o Contain the affected area o Remove and discard dry wall, particle board, carpet, and other porous materials in the affected areas as well as an additional 2 feet in each direction.

o Visually inspect studs within walls; wood with indications of rot should be removed o Treat studs, hard woods, and other non-porous materials with a biocide o Where practical, cover the affected area(s) with Killz or other anti-fungal paint o Dry and HEPA-Vac the area o Have tests run by a company independent to the remediator to ensure mold levels are within normal or expected levels after the remediation is completed.

Damp indoor environments have been associated with many serious health effects, including asthma, hypersensitivity, and sinusitis. Moisture incursion leading to dampness can result from water leaks and/or by condensation due to high humidity. Common sources of moisture in buildings include: plumbing; roof and window leaks; flooding; condensation on cold surfaces;

e.g, pipe sweating; poorly maintained drain pans; and wet foundations due to landscaping or gutters that direct water into or under the building. Water vapor from unvented or poorly vented areas can also create conditions that promote microbial growth.

Management of moisture requires proper control of temperatures and ventilation to avoid high humidity, condensation on surfaces, and excess moisture in materials. ASHRAE recommends relativity humidity levels between 30 and 60 percent for optimum comfort. Higher humidity may result in microbial growth. A consistently implement good-house-keeping plan essential to inhibit or reduce the microbial growth in the facility.

Based on the physical investigation and sample results, several areas of concern were noted at the facility. Moisture intrusion is originating from several structural damages. Before the mold remediation, it is important fix the roof and other damages areas to avoid moisture and fungal growth.

Appendix A Site Location

Aerial View of the Facility

Appendix B Equipment Specifications

Equipment Specs:

Kit includes:

• MegaLite Pump with mounted Rotameter and Case with Custom foam.

• Allergenco-D

• High volume Pump with Rotameter and Case with Custom foam.

• One Stage Andersen

• Agar Plate for Mold

Appendix C Environmental Microbiology Laboratory Test Results

LABORATORY REPORT

Fungal Characterization - Air

CEI Lab Code: I17-2452

Date Received: 11-29-17

Date Analyzed: 12-07-17

Date Reported: 12-08-17

Lab ID

Volume

(Liters)

Culture Method

Client: Altol Environmental Services

PO Box 359

Mecedita, P.R. 00715

Project: Job Corps Arecibo; ENJ-3157

Fungal IDClient ID

Colony

Counts

Concentration

(CFU/m

Percentage*

Cladosporium 41 547 89

Sterilia mycelia 5 67 11

M74537 753157-112717-

MI-01

610TOTAL:13Limit of Detection: 100%

Cladosporium 88 1173 95

Sterilia mycelia 5 67 5

M74538 753157-112717-

MI-02

1200TOTAL:13Limit of Detection: 100%

Cladosporium 17 227 55

Penicillium 5 67 16

Aspergillus niger 4 53 13

Sterilia mycelia 4 53 13

Bipolaris/Drechslera 1 13 3

M74539 753157-112717-

MI-03

410TOTAL:13Limit of Detection: 100%

Cladosporium 18 240 53

Penicillium 8 107 24

Sterilia mycelia 6 80 18

Aspergillus niger 2 27 6

M74540 753157-112717-

MI-04

450TOTAL:13Limit of Detection: 100%

Cladosporium 25 333 64

Sterilia mycelia 7 93 18

Penicillium 4 53 10

Fusarium 2 27 5

Aspergillus niger 1 13 3

M74541 753157-112717-

MI-05

520TOTAL:13Limit of Detection: 100%

Cladosporium 67 893 91

Sterilia mycelia 6 80 8

Curvularia 1 13 1

M74542 753157-112717-

MI-06

990TOTAL:13Limit of Detection: 100%

Cladosporium 72 960 89

Sterilia mycelia 8 107 10

Penicillium 1 13 1

M74543 753157-112717-

MI-07

1100TOTAL:13Limit of Detection: 100%

Page 1 of 2CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

LABORATORY REPORT

Fungal Characterization - Air

CEI Lab Code: I17-2452

Date Received: 11-29-17

Date Analyzed: 12-07-17

Date Reported: 12-08-17

Lab ID

Volume

(Liters)

Culture Method

Client: Altol Environmental Services

PO Box 359

Mecedita, P.R. 00715

Project: Job Corps Arecibo; ENJ-3157

Fungal IDClient ID

Colony

Counts

Concentration

(CFU/m

Percentage*

Cladosporium 43 573 74

Sterilia mycelia 10 133 17

Aspergillus niger 3 40 5

Penicillium 2 27 3

M74544 753157-112717-

MI-08

770TOTAL:13Limit of Detection: 100%

Cladosporium 35 467 87

Sterilia mycelia 5 67 13

M74545 753157-112717-

MI-09

530TOTAL:13Limit of Detection: 100%

Cladosporium 63 840 85

Sterilia mycelia 11 147 15

M74546 753157-112717-

MI-10

990TOTAL:13Limit of Detection: 100%

Cladosporium 54 720 71

Sterilia mycelia 14 187 18

Fusarium 5 67 7

Penicillium 3 40 4

M74547 753157-112717-

MI-11

1000TOTAL:13Limit of Detection: 100%

Samples were received in acceptable condition.

Media used: Malt extract agar (MEA) Limit of Detection=1000/Volume Sampled

*Percentage of each group of fungi in total population. Concentration=LoD X Colony Counts

Concentrations below the Limit of Detection will be reported as ND (None Detected)

Note: Concentration (CFU/m ) is reported to 2 significant figures.

The above results relate only to the items tested, and cannot be extrapolated to anything larger than their original intent. Also, these results cannot be interpreted without physical inspection and consideration for the building's characteristics and factors that may have led to its condition.

This report may not be reproduced, except in full, without written approval by CEI LABS. CEI LABS makes no warranty representation regarding the accuracy of client submitted information in preparing and presenting analytical results.

ANALYST: APPROVED BY:

Tianbao Bai, Ph.D.

Laboratory Director

Marti Bowers

Page 2 of 2CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

MOLD SPORE TRAP REPORT

Nonviable Direct Microscopy

Prepared for

Altol Environmental Services

TEL: 866-481-1412

www.ceilabs.com

CLIENT PROJECT:

CEI LAB CODE:

TEST METHOD: CEI Method 110

RECEIVED DATE:

REPORT DATE:

Job Corps Arecibo; ENJ-3157

I17-2451

12/01/17

11/29/17

All samples received in acceptable condition. Analytical results are not corrected for field and laboratory blanks.

Test results relate only to the items tested and cannot be extrapolated to anything larger than their original intent. This report may not be reproduced, except in full, without written approval by CEI Labs, Inc (CEI). CEI bears no responsibility for client sampling methods and makes no warranty representation regarding the accuracy of client-supplied information in preparing and presenting analytical results. CEI maintains liability limited to the cost of analysis, except for CEI’s own willful misconduct or gross negligence.

Interpretation of the analytical results is the sole responsibility of the customer.

Tianbao Bai, Ph.D., CIH Laboratory Director

CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

MOLD SPORE TRAP REPORT: NONVIABLE DIRECT MICROSCOPY

CEI Lab Code: I17-2451 Date Received: 11-29-17 Date Analyzed: 12-01-17 Date Reported: 12-01-17

CLIENT

Altol Environmental Services PO Box 359 Mecedita, P.R. 00715

CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

* Heliocomyces includes Helicosporium; ** Periconia/Smuts includes Myxomycetes Spores per m3 ( final counts ) reported to 2 significant figures Spores of Aspergillus, Penicillium, and others are small with few distinguishing features and therefore can not be differentiated.

If % analyzed is <100%, spores per m3 is based on extrapolation and not actual count.

ANALYST: REVIEWED BY:

Tianbao Bai, Ph.D., Laboratory DirectorVidya Natarajan

PROJECT: Job Corps Arecibo; ENJ-3157

100%

Alternaria Arthrinium 1 13 <1100 Ascospores 53 707 9100 47 627 9100 46 613 32100 Basidiospores 33 440 6100 11 147 2100 15 200 11100 Bipolaris/Drechslera 1 13 <1100 4 53 1100 Cercospora 1 13 <1100 5 67 1100 2 27 1100 Curvularia 3 40 1100 11 147 2100 8 107 6100 Epicoccum Helicomyces* Nigrospora Oidium/Peronospora Periconia/Smuts** 3 40 1100 2 27 <1100 7 93 5100 Pithomyces Rusts 1 13 <1100 2 27 1100 Spegazzinia Stemphylium Tetraploa Torula 1 13 <1100 Unspecified spores 2 27 <1100 8 107 2100 9 120 6100

340 7700

Health Service Dpt.

Location

Volume (L)

Lab ID M74526

3157-112717-ST-01

Raw

Counts

Client ID

Spores per m

% of

Total

Total

IDENTIFICATION

P r e d o m in a n tly O u td o o r W a te r

In d ic a to r

In d o o r

O u td o o r

Aspergillus/Penicillium 133 4926 6436 266 3547 52100 15 200 11100 Cladosporium 102 1360 18100 153 2040 30100 31 413 22100 Fusarium 5 67 1100 7 93 5100

Chaetomium Stachybotrys Trichoderma Ulocladium

Analyzed

6800 100%

Retail Sales Dpt.

M74527

3157-112717-ST-02

Raw

Counts

Spores per m

% of

Total

Analyzed

140 1900 100%

Administration Dpt.

Background Debris

Pollen Count

Mycelial Fragments

M74528

3157-112717-ST-03

Raw

Counts

Spores per m

% of

Total

Analytical Sensitivity (Spores/m ) 13 13 13

Analyzed

Date Received: 11-29-17

Date Analyzed: 12-01-17

Date Reported: 12-01-17

CLIENT

Altol Environmental Services

PO Box 359

Mecedita, P.R. 00715

CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

* Heliocomyces includes Helicosporium; ** Periconia/Smuts includes Myxomycetes Spores per m3 ( final counts ) reported to 2 significant figures Spores of Aspergillus, Penicillium, and others are small with few distinguishing features and therefore can not be differentiated.

If % analyzed is <100%, spores per m3 is based on extrapolation and not actual count.

ANALYST: REVIEWED BY:

Tianbao Bai, Ph.D., Laboratory DirectorVidya Natarajan

PROJECT: Job Corps Arecibo; ENJ-3157

100%

Alternaria Arthrinium Ascospores 29 387 6100 45 600 35100 78 1040 25100

Basidiospores 8 107 2100 17 227 13100 32 427 10100

Bipolaris/Drechslera 2 27 2100

Cercospora 4 53 1100

Curvularia 2 27 <1100 1 13 1100 12 160 4100

Epicoccum Helicomyces* Nigrospora Oidium/Peronospora Periconia/Smuts** 1 13 <1100 21 280 16100 9 120 3100

Pithomyces Rusts 1 13 1100

Spegazzinia Stemphylium Tetraploa Torula Unspecified spores 22 293 5100 8 107 6100 5 67 2100

200 6000

Sport Pavilion Location

Volume (L)

Lab ID M74529

3157-112717-ST-04

Raw

Counts

Client ID

Spores per m

% of

Total

Total

IDENTIFICATION

P r e d o m in a n tly O u td o o r W a te r

In d ic a to r

In d o o r

O u td o o r

Aspergillus/Penicillium 104 4782 8029 8 107 6100 91 1213 30100

Cladosporium 25 333 6100 24 320 19100 73 973 24100

Fusarium 4 53 1100 2 27 2100 2 27 1100

Chaetomium Stachybotrys Trichoderma Ulocladium

Analyzed

1700 100%

Outside Area

M74530

3157-112717-ST-05

Raw

Counts

Spores per m

% of

Total

Analyzed

310 4100 100%

CTT Manager Office

Background Debris

Pollen Count

Mycelial Fragments

M74531

3157-112717-ST-06

Raw

Counts

Spores per m

% of

Total

Analytical Sensitivity (Spores/m ) 13 13 13

Date Received: 11-29-17

Date Analyzed: 12-01-17

Date Reported: 12-01-17

CLIENT

Altol Environmental Services

PO Box 359

Mecedita, P.R. 00715

CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

* Heliocomyces includes Helicosporium; ** Periconia/Smuts includes Myxomycetes Spores per m3 ( final counts ) reported to 2 significant figures Spores of Aspergillus, Penicillium, and others are small with few distinguishing features and therefore can not be differentiated.

If % analyzed is <100%, spores per m3 is based on extrapolation and not actual count.

ANALYST: REVIEWED BY:

Tianbao Bai, Ph.D., Laboratory DirectorVidya Natarajan

PROJECT: Job Corps Arecibo; ENJ-3157

100%

Alternaria Arthrinium Ascospores 16 213 2100 25 333 10100 38 507 16100

Basidiospores 23 307 3100 3 40 1100 7 93 3100

Bipolaris/Drechslera 1 13 <1100

Cercospora 7 93 1100 11 147 4100 5 67 2100

Curvularia 43 573 5100 9 120 3100 103 1373 43100

Epicoccum Helicomyces* Nigrospora Oidium/Peronospora Periconia/Smuts** 27 360 3100 1 13 <1100 7 93 3100

Pithomyces 2 27 <1100 2 27 1100

Rusts 2 27 1100 3 40 1100

Spegazzinia 2 27 <1100

Stemphylium Tetraploa Torula 2 27 <1100 2 27 1100

Unspecified spores 18 240 2100 4 53 2100 6 80 3100

420 12000

Office Administration Classroom Location

Volume (L)

Lab ID M74532

3157-112717-ST-07

Raw

Counts

Client ID

Spores per m

% of

Total

Total

IDENTIFICATION

P r e d o m in a n tly O u td o o r W a te r

In d ic a to r

In d o o r

O u td o o r

Aspergillus/Penicillium 187 8598 7429 100 1333 39100 2 27 1100

Cladosporium 81 1080 9100 89 1187 35100 65 867 27100

Fusarium 8 107 1100 12 160 5100 1 13 <1100

Chaetomium 1 13 <1100

Stachybotrys Trichoderma Ulocladium

Analyzed

3400 100%

Male Residence - RA Office B

M74533

3157-112717-ST-08

Raw

Counts

Spores per m

% of

Total

Analyzed

240 3200 100%

Residential Life Management Office

Background Debris

Pollen Count

Mycelial Fragments

M74534

3157-112717-ST-09

Raw

Counts

Spores per m

% of

Total

Analytical Sensitivity (Spores/m ) 13 13 13

Date Received: 11-29-17

Date Analyzed: 12-01-17

Date Reported: 12-01-17

CLIENT

Altol Environmental Services

PO Box 359

Mecedita, P.R. 00715

CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

* Heliocomyces includes Helicosporium; ** Periconia/Smuts includes Myxomycetes Spores per m3 ( final counts ) reported to 2 significant figures Spores of Aspergillus, Penicillium, and others are small with few distinguishing features and therefore can not be differentiated.

If % analyzed is <100%, spores per m3 is based on extrapolation and not actual count.

ANALYST: REVIEWED BY:

Tianbao Bai, Ph.D., Laboratory DirectorVidya Natarajan

PROJECT: Job Corps Arecibo; ENJ-3157

100%

Alternaria Arthrinium Ascospores 13 173 4100 7 93 1100

Basidiospores 4 53 1100 6 80 1100

Bipolaris/Drechslera 1 13 <1100

Cercospora 7 93 2100 2 27 <1100

Curvularia 38 507 10100 10 133 1100

Epicoccum 1 13 <1100

Helicomyces* Nigrospora Oidium/Peronospora Periconia/Smuts** 1 13 <1100 2 27 <1100

Pithomyces Rusts 2 27 1100

Spegazzinia Stemphylium Tetraploa Torula 3 40 1100

Unspecified spores 2 27 <1100

360 4800

Female Residence Lobby Location

Volume (L)

Lab ID M74535

3157-112717-ST-10

Raw

Counts

Client ID

Spores per m

% of

Total

Total

IDENTIFICATION

P r e d o m in a n tly O u td o o r W a te r

In d ic a to r

In d o o r

O u td o o r

Aspergillus/Penicillium 71 947 20100 110 6984 7521

Cladosporium 212 2827 58100 140 1867 20100

Fusarium 10 133 3100 6 80 1100

Chaetomium Stachybotrys Trichoderma Ulocladium

Analyzed

9300 100%

Licensed Practical Nurse Classroom

M74536

3157-112717-ST-11

Raw

Counts

Spores per m

% of

Total

Analyzed

Background Debris

Pollen Count

Mycelial Fragments

Raw

Counts

Spores per m

% of

Total

Analytical Sensitivity (Spores/m ) 13 13

Page 6 of 7CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

For purposes of this report, identified mold spores are classified into three general categories depending on environmental conditions the spore is most commonly associated with:

1) PREDOMINANTLY OUTDOOR: Most commonly found growing outdoors and are not usually associated with indoor mold sources.

2) INDOOR / OUTDOOR: Commonly grow in both indoor and outdoor environments.

3) WATER INDICATOR: Most commonly associated with indoor mold growth in buildings with long-term water intrusion issues.

Background debris is the amount of non-biological particulates present in the trace including dust, fibers, skin scales, dust mites, and insect parts. A debris rating is assigned each trace from 0 (lowest) to 5 (highest). A higher debris rating means samples are more difficult to analyze, and spores, especially smaller spores like Aspergillus / Penicilium, may be obscured. Counts with debris ratings of 4 or 5 should be regarded as minimal counts with actual counts assumed to be significantly higher. A further explanation of the debris rating is listed below:

0 - None Detected. No debris observed.

1 - Trace. Field of view obscured < 5%. Counts unaffected.

2 - Light. Field of view obscured 5% to 25%. Counts slightly affected.

3 - Moderate. Field of view obscured 25% to 75% . Actual counts may be higher than reported counts.

4- Heavy. Field of view obscured 75% to 90% . Actual counts may be significantly higher than reported counts.

5 - Very Heavy. Field of view obscured > 90% . Actual counts may be significantly higher than reported counts. Resampling may be necessary.

Analytical Sensitivity: Spore per cubic meter (concentration) divided by raw count.

Limit of Detection: One Spore

Mycelial Fragments: Mycelial fragments are broken pieces of fungal hyphae and constitute the vegetative structure of the fungus.

Pollen Count: Pollen grains (Pollen) are the male reproductive structures of Angiosperm plants. These are counted only as pollen and not classified to Genus level.

Raw Counts: The number of spores counted by the analyst.

% Analyzed: The amount of the trace that was analyzed for each individual spore type. If large amounts of any spore type(s) exist, counts may be extrapolated.

% of Total: Percentage of the sample that is made up of each spore type.

There are no current Federal standards regarding permissible levels of airborne fungi that may be present in buildings. Mold spores are ubiquitous to our planet and it is expected that some spores will be present in normal indoor environments. A general guideline that is widely accepted in the industrial hygiene industry is that the types and numbers of mold spores present in the indoor environment should be similar to those present in the outdoor environment. If inside spore counts are significantly higher than outside counts this may indicate a potential mold problem. The comparison of outdoor and indoor spore types and concentrations is a useful tool in assessing abnormal mold contamination;

however, it should not be the sole determining factor in evaluating health risks and remediation strategies.

SPORE CLASSIFICATION:

BACKGROUND DEBRIS:

DEFINITION OF TERMS:

INDOOR AND OUTDOOR COMPARISONS:

PREDOMINANTLY

OUTDOOR INDOOR / OUTDOOR WATER

INDICATOR

SPORE INFORMATION

Page 7 of 7CEI Labs, 730 SE Maynard Road, Cary, NC 27511, Phone: (866) 481-1412

Alternaria Soil, seeds, plants, carpet, textiles, window frames, air X X

Arthrinium Soil, plant materials, decaying wood X

Ascospores Plants, soil, cellulose-containing materials, air

Basidiospores Soil, plants, wood, cellulose-containing materials, air

Bipolaris/Drechslera Grasses, plant material, decaying food, soil

Cercospora Plants

Curvularia Soil, plant materials, cellulose-containing materials X

Epicoccum Plants, soil, seeds, carpet, air X

Helicomyces* Plants

Nigrospora Plants, soil

Oidium/Peronospora Plants

Periconia/Smuts** Plants, air X

Pithomyces Soil, plant material, air

Rusts Grasses, trees, other plants X

Spegazzinia Soil, plants

Stemphylium Dead plants, cellulose-containing materials

Tetraploa Plants

Torula Soil, plants

Unspecified spores Various

Predom inantly O utdoor W ater Indicator

Indoor / O utdoor

SPORE NAME

* Heliocomyces includes Helicosporium; * Periconia/Smuts includes Myxomycetes

COMMON HABITAT ALLERGENIC

POTENTIAL

MYCOTOXIN

POTENTIAL

Aspergillus/Penicillium Soil, food, carpet, HVAC, air X X

Cladosporium Plants, woody plants, food, soil, paint, textiles, carpet, HVAC, air X

Fusarium Soil, plants, seed, fruits, grains X

Chaetomium Cellulose-containing materials, soil, seeds, dung X X

Stachybotrys Paper, wallpaper, gypsum board X X

Trichoderma Soil, decaying wood, plant material, cellulose-containing materials X X

Ulocladium Soil, grasses, wood, paper

Appendix D Mold Inspection and Testing Photos

Health and wellness department

Mold stain, ceiling tile, Health & Wellness Dpt.

Retail Sales

Retail Sales

Administration Department

Administration Department

Mold on floor, Administraton Dpt.

Sport Pavillion

Mold on chairs, Sport Pavillion

Mold on table pool

CTT Manager Office

Mold on gypsum board, CTT Manager Office

Office Administration Classroom

Structural damage on roof, Office Administration Classroom

Structural Damage, Office Administration Clasroom

Male Residence, chair with mold

Male Residence RA B Office

Male Residence

Residential living management office

Structural damage, Residential living management office

Female Residence, lobby

Mold on furniture, Female Residence

Licensed Practical Nurse Classroom

Licensed Practical Nurse Classroom

Structural damage, Licensed practical nurse classroom

Water intrusion, Licensed practical nurse classroom