Merhab- dcmf metabarcoding and qpcr
Closed Solicitation Posted
- Solicitation number
- 140G0326Q0123
- Agency
- Office of Acquisitions and Grants US Geological Survey, Department of the Interior
- Responses due
- Set-aside
- Total Small Business
Opportunity facts
- NAICS code
- 541380 Testing Laboratories and Services
- PSC
- F999 Other Environmental Services
- Points of contact
-
- Elizabeth Adam eadam@usgs.gov (916) 278-9441
Notice details come from SAM.gov. Updated .
About this opportunity
The U.S. Geological Survey, California Water Science Center, is soliciting quotations for quantitative polymerase chain reaction (qPCR) and digital droplet PCR (ddPCR) analyses of cyanobacteria DNA, with quotes due May 27, 2026, at 10:00 AM Pacific Daylight Time. This is a total small business set-aside under FAR Part 12 commercial items procedures.
The NAICS code is 541380, Testing Laboratories and Services, with a $19.0 million size standard. PSC code is F999, Other Environmental Services. All services will be performed at the contractor's laboratory. The estimated period of performance runs from July 1, 2026, through September 30, 2027.
Specific deliverables include DNA extraction from 30 filter samples, metabarcoding analysis of 16S V4 (50 samples), 18S V7 (30 samples), and 18S V4 (20 samples), qPCR analysis for Microcystis mcyE and cpcB genes (50 samples each), ddPCR analysis for mcyE and cpcB genes (30 samples each), DNA archive for future analyses, and bioinformatic code in text format. Data must be delivered as XLS, CSV, or TXT files with summary documentation by September 30, 2027. Samples are drawn from the San Francisco Bay-Delta region.
The laboratory leader must hold a Ph.D. in a cyanobacterial ecology-related field with demonstrated expertise in molecular methods applied to cyanobacteria and cyanotoxin production, and prior experience with Sacramento-San Joaquin Delta samples. Service Contract Labor Standards wage determinations apply based on contractor location. Evaluation factors include technical expertise, price, and past performance on a tradeoff basis. Questions are due May 25, 2026, at 10:00 AM PDT to elizabeth_adam@ios.doi.gov. Quotations must be submitted by email to the same address; hardcopy and web portal submissions will not be accepted.
Notice text
REQUEST FOR QUOTE
CYANOBACTERIA DNA ANALYSIS
INTRODUCTION
This is a solicitation for commercial items prepared in accordance with the format in Federal Acquisition Regulation (FAR) Part 12 as supplemented with additional information included in this notice.
SMALL BUSINESS SET-ASIDE
DESCRIPTION
The U.S. Geological Survey (USGS), California Water Science Center (CAWSC), requires quantitative polymerase chain reaction (qPCR) and digital droplet PCR (ddPCR) analyses of cyanobacteria DNA as described in the attached Statement of Work (SOW).
RELEVANT INFORMATION
The North American Industry Classification System (NAICS) 541380, Testing Laboratories and Services, and associated size standard $19.0 million applies to this announcement, along with Product Service Code F999, Other Environmental Services.
This service is subject to the Service Contract Labor Standards Wage Determinations. Wage Determination # TBD based on the contractor's location.
All services will be performed at the contractor's laboratory.
QUESTIONS
Questions must be sent directly to elizabeth_adam@ios.doi.gov by Monday, May 25, 2026, at 10:00am PD.
SUBMISSION OF QUOTES
Quotes are due on Wednesday, May 27, 2026, at 10:00am PD. Quotes shall only be accepted through electronic mail addressed to the Contracting Officer at elizabeth_adam@ios.doi.gov. Quotes submitted by hardcopy or any web portal shall not be accepted or considered
EVALUATION OF QUOTES
Quotes will be evaluated by Tradeoff. Evaluation factors include Technical Expertise, Price, and Past Performance.
Offerors are to include a technical description of the services being offered in sufficient detail to evaluate compliance with the requirements in the solicitation as seen in the Evaluation Factors (see Attachment). This may include a work plan, CV, references, or other documents as necessary.
ESTIMATED PERIOD OF PERFORMANCE
07/01/2026 - 09/30/2027
Offerors shall return the following:
Signed SF-18
Firm, fixed price quote
Technical information including work plan
System for Award Management (SAM) Unique Identification Entity (UEI)
Attached Pages:
- Statement of Work
- Clauses and Provisions
Attachments
| File | Type | Posted |
|---|---|---|
| Sol_140G0326Q0123.pdf |
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