LOOF_2024_Annual_Report_Part4.pdf
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- Solicitation number
- AU-2026-0000952
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- Alabama
About this file
This file is an annual report from a laboratory testing facility (LOOF) documenting standard reference toxicant testing results and organism history for environmental testing protocols. The document details chronic reference tests performed on two aquatic species commonly used in toxicity assessments: Pimephales promelas (Fathead minnow) and Ceriodaphnia dubia (water flea). The Fathead minnow chronic reference test was conducted from November 26, 2024 to December 3, 2024, yielding a Survival LC-50 of 2,592 mg/l and Growth IC-25 of 1,723 mg/l with a PMSD of 12.7. The Ceriodaphnia dubia chronic reference test was performed from November 12, 2024 to November 18, 2024, producing a Survival LC-50 of 1,789 mg/l and Reproduction IC-25 of 795.2 mg/l with a PMSD of 25.3. Both tests utilized sodium chloride in synthetic moderately hard water as the reference toxicant standard.
The document also contains extensive organism history records for both test species, documenting detailed information about individual organisms used in testing protocols, including acquisition dates, ages, and laboratory identification codes. The remainder of the report consists of technical data tables and reference documentation related to organism maintenance and handling procedures. This annual report serves as documentation of the laboratory's quality assurance protocols and adherence to standard testing methodologies in environmental toxicology, which would be relevant to regulatory compliance for environmental remediation and water quality assessment activities.
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IV. Standard Reference Toxicants Sodium chloride in synthetic moderately hard water.
Pimephales promelas (Fathead minnow) A chronic reference test was performed on November 26, 2024 at 1451 to December 03, 2024 at 1332 The results were as follows: (Control No. 275337-1.)
Survival LC-50: 2592 mg/l Growth IC-25: 1723 mg/l Growth PMSD: 12.7
Ceriodaphnia dubia A chronic reference test was performed on November 12, 2024 at 1149 to November 18, 2024 at 1321 The results were as follows: (Control No. 275337-2.)
Survival LC-50: 1789 mg/l Reproduction IC-25: 795.2 mg/l Reproduction PMSD: 25.3
V. Organism History
Pimephales promelas (Fathead minnow) Date: December 10, 2024 Age: <24 hours Source: In-house culture Water: Moderately hard synthetic Temperature: 25 deg.C
Ceriodaphnia dubia Date: December 10, 2024 Age: <24 hours Source: In-house culture Water: Moderately hard synthetic Temperature: 25 deg.C
Page 7 of 42 12/26/2024
Larvae are exposed in a static renewal system for seven days to different concentrations of effluent with dilution water. Test results are based on the survival and growth (weight) of the larvae.
VI. Results Summary Pimephales promelas, Fathead minnow Larval Survival and Growth Test -- Method 1000.0
Effluent concentrations for this test were 10 %, 18 %, 32 %, 56 %, 100 % in accordance with the NPDES permit.
The test was initiated on December 10, 2024 at 1438 and continued through December 17, 2024 at 1422.
Statistical analyses were performed on the observed data and the no observable effects concentrations (NOECs) were as follows:
a.) NOEC survival = 100 % effluent b.) NOEC growth = 100 % effluent
Summary of the 7-day Fathead Minnow Survival and Growth
Mean Growth (mg)Concentration Percent Survival 0.877Control 92.5
0.94210 % 97.5
0.96618 % 95.0
0.87532 % 77.5
1.0056 % 92.5
0.945100 % 90.0
10.00 18.00 32.00 56.00 100.00 Concentration (%)
S u rv iv a l
Page 8 of 42 12/26/2024
Neonates are exposed in a static renewal system to different concentrations of effluent with dilution water until 60% of surviving control organisms have three broods of offspring or a maximum of eight test days.
VI. Results Summary Ceriodaphnia dubia, Cladoceran Survival and Reproduction Test -- Method 1002.0
Effluent concentrations for this test were 10 %, 18 %, 32 %, 56 %, 100 % in accordance with the NPDES permit.
The test was initiated on December 10, 2024 at 1427 and continued through December 16, 2024 at 1354.
Statistical analyses were performed on the observed data and the no observable effects concentrations (NOECs) were as follows:
a.) NOEC survival = 100 % effluent b.) NOEC reproduction = 32 % effluent
Summary of the 6-day Ceriodaphnia dubia Survival and Reproduction Data
Concentration Mean ReproductionPercent Survival Control 31.5100 10 % 32.9100 18 % 32.6100 32 % 32.0100 56 % 27.0100 100 % 5.80100
10.00 18.00 32.00 56.00 100.00 Concentration (%)
S u rv iv a l
Page 9 of 42 12/26/2024
Concentration Replicate
Appendix (Data): Test 1000.0
Pimephales promelas (Fathead Minnow) 7-Day Survival
Date and Time Test Initiated:
Date and Time Test Terminated:
Number of Survivors Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
December 10, 2024 at 1438 December 17, 2024 at 1422
Control A 10 10 10 10 10 10 10
B 10 10 10 10 10 10 10
C 10 10 10 9 8 8 8
D 10 10 9 9 9 9 9
10 % A 10 10 10 10 10 10 10
B 10 10 10 10 10 10 10
C 10 10 10 10 9 9 9
D 10 10 10 10 10 10 10
18 % A 9 9 9 9 9 9 9
B 10 10 10 9 9 9 9
C 10 10 10 10 10 10 10
D 10 10 10 10 10 10 10
32 % A 10 9 9 8 8 7 7
B 10 10 10 10 9 7 7
C 10 9 9 9 9 9 9
D 10 9 9 9 9 9 8
56 % A 10 10 10 10 10 10 10
B 10 10 8 8 8 8 8
C 10 10 10 10 10 10 10
D 10 10 10 10 9 9 9
100 % A 10 10 10 10 10 10 10
B 10 10 9 9 9 8 8
C 10 9 9 8 8 8 8
D 10 10 10 10 10 10 10
Page 10 of 42 12/26/2024
Concentration Replicate Weight of pan + fish
Total weight of fish (g)
Original # of fish
Mean dry weight (mg)
Appendix (Data): Test 1000.0
Pimephales promelas (Fathead Minnow) 7-Day Growth
Test Initiated:
Test Terminated:
Weight of pan
December 10, 2024 at 1438 December 17, 2024 at 1422
Control A .81499 .82509 0.01010 10 1.010
B .80884 .81782 0.00898 10 0.898
C .82317 .83089 0.00772 10 0.772
D .82290 .83117 0.00827 10 0.827
10 % A .82929 .83899 0.00970 10 0.970
B .83429 .84341 0.00912 10 0.912
C .81936 .82850 0.00914 10 0.914
D .82730 .83703 0.00973 10 0.973
18 % A .80645 .81539 0.00894 10 0.894
B .84085 .84883 0.00798 10 0.798
C .81081 .82170 0.01089 10 1.090
D .83745 .84825 0.01080 10 1.080
32 % A .80562 .81399 0.00837 10 0.837
B .80580 .81461 0.00881 10 0.881
C .80139 .81154 0.01015 10 1.020
D .80999 .81761 0.00762 10 0.762
56 % A .81632 .82690 0.01058 10 1.060
B .81595 .82440 0.00845 10 0.845
C .81452 .82489 0.01037 10 1.040
D .81527 .82585 0.01058 10 1.060
100 % A .80974 .81948 0.00974 10 0.974
B .81521 .82485 0.00964 10 0.964
C .82537 .83319 0.00782 10 0.782
D .82694 .83752 0.01058 10 1.060
Page 11 of 42 12/26/2024
Appendix (Data): Test 1002.0
Ceriodaphnia dubia Survival and Reproduction
Date and Time Test Initiated:
Date and Time Test Terminated:
December 10, 2024 at 1427 December 16, 2024 at 1354
Concentration: Control
Day No. of Adults
Young per Adult
No. of Young1 2 3 4 5 6 7 8 9 10
Replicate
1 0 0 0 0 0 0 0 0 0 0 0 10 0.00 2 0 0 0 0 0 0 0 0 0 0 0 10 0.00 3 6 3 4 3 0 5 4 4 3 4 36 10 3.60 4 14 1 0 1 2 1 0 0 0 11 30 10 3.00 5 0 11 12 11 0 15 13 12 12 0 86 10 8.60 6 18 21 16 18 11 16 17 15 15 16 163 10 16.3
TOTAL 38 36 32 33 13 37 34 31 30 31 315 10 31.5
Concentration: 10 %
Day No. of Adults
Young per Adult
No. of Young1 2 3 4 5 6 7 8 9 10
Replicate
1 0 0 0 0 0 0 0 0 0 0 0 10 0.00 2 0 0 0 0 0 0 0 0 0 0 0 10 0.00 3 5 6 4 5 3 3 4 3 4 3 40 10 4.00 4 11 0 1 0 2 0 0 11 0 0 25 10 2.50 5 0 12 13 12 14 14 12 0 11 11 99 10 9.90 6 17 19 15 16 17 20 17 15 16 13 165 10 16.5
TOTAL 33 37 33 33 36 37 33 29 31 27 329 10 32.9
Concentration: 18 %
Day No. of Adults
Young per Adult
No. of Young1 2 3 4 5 6 7 8 9 10
Replicate
1 0 0 0 0 0 0 0 0 0 0 0 10 0.00 2 0 0 0 0 0 0 0 0 0 0 0 10 0.00 3 5 3 7 3 3 5 3 3 3 4 39 10 3.90 4 11 0 0 0 0 0 0 0 11 11 33 10 3.30 5 0 11 12 13 13 14 11 13 0 1 88 10 8.80 6 20 15 16 16 17 18 16 17 17 14 166 10 16.6
TOTAL 36 29 35 32 33 37 30 33 31 30 326 10 32.6
Page 12 of 42 12/26/2024
Appendix (Data): Test 1002.0
Ceriodaphnia dubia Survival and Reproduction
Date and Time Test Initiated:
Date and Time Test Terminated:
December 10, 2024 at 1427 December 16, 2024 at 1354
Concentration: 32 %
Day No. of Adults
Young per Adult
No. of Young1 2 3 4 5 6 7 8 9 10
Replicate
1 0 0 0 0 0 0 0 0 0 0 0 10 0.00 2 0 0 0 0 0 0 0 0 0 0 0 10 0.00 3 4 3 4 5 4 5 3 5 4 5 42 10 4.20 4 13 0 1 0 1 0 0 0 0 0 15 10 1.50 5 0 13 12 14 11 15 12 11 11 9 108 10 10.8 6 16 18 14 16 14 16 14 16 15 16 155 10 15.5
TOTAL 33 34 31 35 30 36 29 32 30 30 320 10 32.0
Concentration: 56 %
Day No. of Adults
Young per Adult
No. of Young1 2 3 4 5 6 7 8 9 10
Replicate
1 0 0 0 0 0 0 0 0 0 0 0 10 0.00 2 0 0 0 0 0 0 0 0 0 0 0 10 0.00 3 4 3 5 4 5 3 4 3 3 4 38 10 3.80 4 10 0 1 0 6 8 0 0 0 0 25 10 2.50 5 7 8 11 12 5 0 10 11 8 11 83 10 8.30 6 15E 12 15 14 14 13 18 13 12 13 124 10 12.4
TOTAL 21 23 32 30 30 24 32 27 23 28 270 10 27.0
E = Excluded fourth brood neonates
Concentration: 100 %
Day No. of Adults
Young per Adult
No. of Young1 2 3 4 5 6 7 8 9 10
Replicate
1 0 0 0 0 0 0 0 0 0 0 0 10 0.00 2 0 0 0 0 0 0 0 0 0 0 0 10 0.00 3 3 0 0 3 0 3 0 0 0 0 9 10 0.900 4 0 0 0 0 3 0 0 0 0 0 3 10 0.300 5 7 0 0 2 0 0 5 0 0 3 17 10 1.70 6 6 0 0 6 2 3 6 0 2 4 29 10 2.90
TOTAL 16 0 0 11 5 6 11 0 2 7 58 10 5.80
Page 13 of 42 12/26/2024
Appendix (Reference Toxicant): Test 1000.0
Chronic Reference Toxicant, Pimephales promelas (Fathead Minnow)
LC50 Survival Data
Log(LC50) Average Log(LC50) Lower Limit Upper Limit
3.25
3.30
3.35
3.40
3.45
3.50
3.55
3.60
3.65
4/ 4/
5/ 2/
6/ 1/
7/
/2
8/ 1/
9/ 5/
/2
/2
/2
1/
1/ 4/
1/
/2
2/
/2
3/ 5/
4/ 2/
5/
/2
6/ 4/
7/ 2/
8/
/2
9/ 3/
/1
/2
/2
6/
L o g (L
C
IC25 Growth Data
Log(IC25) Average Log(IC25) Lower Limit Upper Limit
2.9
3.1
3.2
3.3
3.4
3.5
3.6
4/ 4/
5/ 2/
6/ 1/
7/
/2
8/ 1/
9/ 5/
/2
/2
/2
1/
1/ 4/
1/
/2
2/
/2
3/ 5/
4/ 2/
5/
/2
6/ 4/
7/ 2/
8/
/2
9/ 3/
/1
/2
/2
6/
L o g (I
C
Page 14 of 42 12/26/2024
Appendix (Reference Toxicant): Test 1002.0
Chronic Reference Toxicant, Ceriodaphnia dubia
3.18
3.20
3.22
3.24
3.26
3.28
3.30
3.32
5/ 2/
6/ 1/
7/
/2
8/ 1/
9/
/2
/3
/2
/2
1/
/1
9/
1/ 4/
2/
/2
2/
/2
3/ 5/
4/ 2/
5/ 2/
6/ 4/
7/ 2/
8/
/2
9/ 3/
/1
/2
/1
2/
LC50 Survival Data
Log(LC50) Average Log(LC50) Lower Limit Upper Limit
L o g (L
C
2.84
2.88
2.92
2.96
3.04
3.08
3.12
3.16
3.20
5/ 2/
6/ 1/
7/
/2
8/ 1/
9/
/2
/3
/2
/2
1/
/1
9/
1/ 4/
2/
/2
2/
/2
3/ 5/
4/ 2/
5/ 2/
6/ 4/
7/ 2/
8/
/2
9/ 3/
/1
/2
/1
2/
IC25 Reproduction Data
Log(IC25) Average Log(IC25) Lower Limit Upper Limit
L o g (I
C
Page 15 of 42 12/26/2024
SUMMARY REPORTING FORMS
CHRONIC BIOMONITORING
Pimephales promelas (Fathead Minnow)
SURVIVAL AND GROWTH
Appendix (Summary): Test 1000.0
Dilution water used:
Date and Time Test Terminated: December 17, 2024 at 1422 Date and Time Test Initiated: December 10, 2024 at 1438
NPDES No.: NA
Permittee: ECC
DATA TABLE FOR SURVIVAL
Percent Survival in replicate chambers
Mean percent survivalEffluent
Conc. % A B C D 24 hr 48 hr 7 days CV% Control 100 100 80.0 90.0 100 100 92.5 10.4 10 % 100 100 90.0 100 100 100 97.5 5.13 18 % 90.0 90.0 100 100 97.5 97.5 95.0 6.08 32 % 70.0 70.0 90.0 80.0 100 92.5 77.5 12.4 56 % 100 80.0 100 90.0 100 100 92.5 10.4 100 % 100 80.0 80.0 100 100 97.5 90.0 12.8
DATA TABLE FOR GROWTH
Effluent Average dry weight, mg replicate chambers Mean dry Conc. % A B C D weight, mg CV% Control 1.010 0.898 0.772 0.827 0.877 11.7 10 % 0.970 0.912 0.914 0.973 0.942 3.59 18 % 0.894 0.798 1.090 1.080 0.966 14.9 32 % 0.837 0.881 1.020 0.762 0.875 12.4 56 % 1.060 0.845 1.040 1.060 1.00 10.4 100 % 0.974 0.964 0.782 1.060 0.945 12.4
CV = Coefficient of variation = standard deviation * 100 / mean
Page 16 of 42 12/26/2024
Appendix (Summary): Test 1000.0
CHRONIC TOXICITY SUMMARY FORM
Pimephales promelas (Fathead minnow)
CHEMICAL PARAMETERS CHART
PERMITTEE:
NPDES NO.:
CONTACT:
ANALYST:
Test Initiated: DATE:
Test Terminated: DATE:
TIME:
TIME:NA
GCX6, V6YL, B6YF, QGL9, WK7B
December 10, 2024 1438ECC
Helen Cavanagh
December 17, 2024 1422
DILUTION DAY DILUTION DAY
1 2 3 4 5 6 7 1 2 3 4 5 6 7 Control 10 %
D.O. Initial 8.9 9.0 8.8 8.9 8.9 9.0 8.8 D.O. Initial 8.9 8.9 9.0 9.0 8.8 8.9 8.8 Final 7.4 6.7 7.1 6.7 6.0 6.9 NA Final 7.2 6.6 7.1 6.6 6.3 7.2 NA pH Initial 8.0 7.8 6.8 7.5 7.9 7.8 8.0 pH Initial 8.0 7.8 7.2 7.7 8.0 7.9 8.0 Final 7.9 7.6 7.7 7.4 7.2 7.3 NA Final 7.6 7.5 7.6 7.4 7.3 7.4 NA
DILUTION DAY DILUTION DAY
1 2 3 4 5 6 7 1 2 3 4 5 6 7 18 % 32 %
D.O. Initial 8.9 9.4 9.0 9.0 8.8 8.9 8.8 D.O. Initial 9.2 9.2 9.1 9.1 8.9 8.9 8.8 Final 7.3 6.7 7.3 7.6 7.1 7.3 NA Final 7.5 6.9 7.2 7.2 6.5 7.6 7.6 pH Initial 8.0 8.0 7.3 7.8 8.0 7.9 8.0 pH Initial 8.0 7.9 7.5 7.9 8.0 7.9 8.0 Final 7.6 7.5 7.6 7.6 7.4 7.5 NA Final 7.6 7.5 7.6 7.5 7.4 7.5 7.8
DILUTION DAY DILUTION DAY
1 2 3 4 5 6 7 1 2 3 4 5 6 7 56 % 100 %
D.O. Initial 9.0 9.4 9.3 9.2 8.9 8.9 8.7 D.O. Initial 9.1 9.4 9.6 9.3 9.2 9.1 9.3 Final 7.5 6.9 7.2 7.2 6.4 7.5 7.6 Final 7.8 7.4 7.3 7.2 6.8 7.8 7.8 pH Initial 8.0 7.9 7.6 7.8 8.0 7.9 8.0 pH Initial 7.9 7.8 7.7 7.9 7.9 7.8 7.9 Final 7.6 7.5 7.6 7.6 7.3 7.5 7.7 Final 7.6 7.5 7.6 7.5 7.4 7.6 7.6
Alkalinity Hardness Conductivity Chlorine Sample ID
59 510 4300 <0.05 OF-EFF-120924 09-DEC-24
64 640 4500 <0.05 OF-EFF-121124 11-DEC-24
64 620 4700 <0.05 OF-EFF-121324 13-DEC-24
Alkalinity Hardness Conductivity Chlorine Sample ID 62 85 290 <0.05 192-16991-A-4 64 97 300 <0.05 192-17230-A-1
Page 17 of 42 12/26/2024
SUMMARY REPORTING FORMS
CHRONIC BIOMONITORING
Ceriodaphnia dubia
SURVIVAL AND REPRODUCTION
Appendix (Summary): Test 1002.0
Dilution water used:
Date and Time Test Terminated: December 16, 2024 at 1354 Date and Time Test Initiated: December 10, 2024 at 1427
NPDES No.: NA
Permittee: ECC
PERCENT SURVIVAL
Percent Effluent Time of Reading Control 18 % 32 % 56 % 100 %10 % 24 hour 100 100 100 100 100100 48 hour 100 100 100 100 100100 6 day 100 100 100 100 100100
NUMBER OF YOUNG PRODUCED PER FEMALE @ 6 DAYS
Percent Effluent Replicates Control 18 % 32 % 56 % 100 %10 % A 38 36 33 21 1633 B 36 29 34 23 037 C 32 35 31 32 033 D 33 32 35 30 1133 E 13 33 30 30 536 F 37 37 36 24 637 G 34 30 29 32 1133 H 31 33 32 27 029 I 30 31 30 23 231 J 31 30 30 28 727 Mean per Adult 31.5 32.6 32.0 27.0 5.8032.9 Mean per Surviving Adult 31.5 32.6 32.0 27.0 5.8032.9
CV % 22.4 8.33 7.51 14.9 95.49.97
CV = Coefficient of variation = standard deviation * 100 / mean (calculated based on young produced by surviving females)
Page 18 of 42 12/26/2024
Appendix (Summary): Test 1002.0
CHRONIC TOXICITY SUMMARY FORM
Ceriodaphnia dubia
CHEMICAL PARAMETERS CHART
PERMITTEE:
NPDES NO.:
CONTACT:
ANALYST:
Test Initiated: DATE:
Test Terminated: DATE:
GCX6, V6YL, B6YF, QGL9, WK7B
NA
TIME:
TIME:
1427ECC
Helen Cavanagh
December 16, 2024 1354
December 10, 2024
Control 10 %
DILUTION DAY DILUTION DAY
1 2 3 4 5 6 7 1 2 3 4 5 6 7 D.O. Initial D.O. Initial 8.9 8.9 9.0 9.0 8.8 8.9 8.88.9 9.0 8.8 8.9 8.9 9.0 8.8 Final Final 9.0 8.8 9.0 8.7 8.9 9.0 --9.0 8.8 9.0 8.7 8.8 9.0 --pH Initial pH Initial 8.0 7.8 7.2 7.7 8.0 7.9 8.08.0 7.8 6.8 7.5 7.9 7.8 8.0 Final Final 8.0 7.3 7.4 7.2 8.2 7.8 --8.0 7.2 7.1 6.9 8.2 7.0 --
18 % 32 %
DILUTION DAY DILUTION DAY
1 2 3 4 5 6 7 1 2 3 4 5 6 7 D.O. Initial D.O. Initial 9.2 9.2 9.1 9.1 8.9 8.9 8.88.9 9.4 9.0 9.0 8.8 8.9 8.8 Final Final 9.0 8.8 9.2 8.7 8.9 9.1 --9.0 8.8 9.0 8.7 8.9 8.9 --pH Initial pH Initial 8.0 7.9 7.5 7.9 8.0 7.9 8.08.0 8.0 7.3 7.8 8.0 7.9 8.0 Final Final 8.1 7.6 8.0 7.7 8.2 8.2 --8.1 7.4 7.6 7.4 8.2 8.0 --
56 % 100 %
DILUTION DAY DILUTION DAY
1 2 3 4 5 6 7 1 2 3 4 5 6 7 D.O. Initial D.O. Initial 9.1 9.4 9.6 9.3 9.2 9.1 9.39.0 9.4 9.3 9.2 8.9 8.9 8.7 Final Final 9.0 8.8 9.0 8.8 8.9 9.0 --9.0 8.8 9.1 8.7 8.8 9.0 --pH Initial pH Initial 7.9 7.8 7.7 7.9 7.9 7.8 7.98.0 7.9 7.6 7.8 8.0 7.9 8.0 Final Final 8.1 8.0 8.2 8.0 8.2 8.3 --8.1 7.8 8.1 8.0 8.2 8.3 --
Alkalinity Hardness Conductivity Chlorine Sample ID
59 510 4300 <0.05 OF-EFF-120924 09-DEC-24
64 640 4500 <0.05 OF-EFF-121124 11-DEC-24
64 620 4700 <0.05 OF-EFF-121324 13-DEC-24
Alkalinity Hardness Conductivity Chlorine Sample ID 62 85 290 <0.05 192-16991-A-4 64 97 300 <0.05 192-17230-A-1
Page 19 of 42 12/26/2024
CETIS Summary Report Report Date: 20 Dec-24 09:13 (p 1 of 2)
Test Code/ID: 275382_CD / 17-7913-6292
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Batch ID: 16-2952-0010
Start Date: 10 Dec-24 14:27
Ending Date: 16 Dec-24 13:54
Test Type: Reproduction-Survival (7d)
Test Length: 5d 23h
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Ceriodaphnia dubia
Source: In-House Culture
Analyst:
Age: <24Taxon: Branchiopoda
Sample ID: 04-5552-4945
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Multiple Comparison Summary
NOEL LOEL TOELEndpointAnalysis ID Comparison Method PMSD✓ STU
100 >100 ---08-1067-3358 ---6d Survival Rate 1Fisher Exact/Bonferroni-Holm Test 1
32 56 42.3300-5155-7244 14.6%Reproduction 3.1Steel Many-One Rank Sum Test 1
95% LCL 95% UCL
Point Estimate Summary
EndpointAnalysis ID Point Estimate Method %Level✓ STU
LC50 >100 --- ---19-6819-5201 <1Linear Interpolation (ICPIN)6d Survival Rate 1
IC25 61.71 54.88 66.0101-8584-7522 1.6Linear Interpolation (ICPIN)Reproduction 1
Test Acceptability
Attribute Test Stat DecisionAnalysis ID Endpoint Lower OverlapUpper
TAC Limits
31.500-5155-7244 15Control RespReproduction Yes Passes Criteria<<
31.501-8584-7522 15Control RespReproduction Yes Passes Criteria<<
0.145600-5155-7244 0.13PMSDReproduction Yes Passes Criteria0.47
Code Mean Min Max Std DevCount CV%Std ErrConc-%
6d Survival Rate Summary
95% LCL 95% UCL %Effect
0 1.0000 1.0000 1.0000 0.000010 0.00%0.00001.0000 1.0000 0.00%D
10 1.0000 1.0000 1.0000 0.000010 0.00%0.00001.0000 1.0000 0.00%
18 1.0000 1.0000 1.0000 0.000010 0.00%0.00001.0000 1.0000 0.00%
32 1.0000 1.0000 1.0000 0.000010 0.00%0.00001.0000 1.0000 0.00%
56 1.0000 1.0000 1.0000 0.000010 0.00%0.00001.0000 1.0000 0.00%
100 1.0000 1.0000 1.0000 0.000010 0.00%0.00001.0000 1.0000 0.00%
Code Mean Min Max Std DevCount CV%Std ErrConc-%
Reproduction Summary
95% LCL 95% UCL %Effect
0 31.5 13 38 7.04410 22.36%2.22726.46 36.54 0.00%D
10 32.9 27 37 3.28110 9.97%1.03830.55 35.25 -4.44%
18 32.6 29 37 2.71610 8.33%0.858930.66 34.54 -3.49%
32 32 29 36 2.40410 7.51%0.760130.28 33.72 -1.59%
56 27 21 32 4.02810 14.92%1.27424.12 29.88 14.29%
100 5.8 0 16 5.53410 95.41%1.751.841 9.759 81.59%
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 20 of 42 12/26/2024
CETIS Summary Report Report Date: 20 Dec-24 09:13 (p 2 of 2)
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
CodeConc-%
6d Survival Rate Detail
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
9B9D24B8DF1F6A22FE98189F611BDCA8MD5:
0 D 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
10 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
18 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
32 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
56 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
100 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
CodeConc-%
Reproduction Detail
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
B6805D4188EB3F0949F7DCC96921DE0DMD5:
0 D 38 36 32 33 13 37 34 31 30 31
10 33 37 33 33 36 37 33 29 31 27
18 36 29 35 32 33 37 30 33 31 30
32 33 34 31 35 30 36 29 32 30 30
56 21 23 32 30 30 24 32 27 23 28
100 16 0 0 11 5 6 11 0 2 7
CodeConc-%
6d Survival Rate Binomials
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
0 D 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
10 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
18 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
32 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
56 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
100 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 21 of 42 12/26/2024
Report Date: 20 Dec-24 09:12 (p 1 of 2)CETIS Analytical Report
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:08 Endpoint: Reproduction CETIS Version: CETISv2.1.4Analysis ID: 00-5155-7244
Analysis: Nonparametric-Control vs Treatments Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: B6805D4188EB3F0949F7DCC96921DE0D Editor ID: 005-847-680-2
Sample ID: 04-5552-4945
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 16-2952-0010
Start Date: 10 Dec-24 14:27
Ending Date: 16 Dec-24 13:54
Test Type: Reproduction-Survival (7d)
Test Length: 5d 23h
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Ceriodaphnia dubia
Source: In-House Culture
Analyst:
Age: <24Taxon: Branchiopoda
Test Stat Critical P-Value Decision(α:5%)TiesvsControl Conc-%
Steel Many-One Rank Sum Test
P-Typedf
106.5 75 4 0.8650 Non-Significant Effect10Dilution Water 18 CDF
100.5 75 6 0.7129 Non-Significant Effect18 18 CDF
94.5 75 6 0.5100 Non-Significant Effect32 18 CDF
73 75 2 0.0318 Significant Effect56* 18 CDF
56 75 0 0.0005 Significant Effect100* 18 CDF
NOEL LOEL Tox UnitsTOELAlt HypData Transform PMSDMSDu
32 56 14.56%3.142.33C > TUntransformed 4.586
Attribute Test Stat Decision
Test Acceptability Criteria
Lower OverlapUpper
TAC Limits
15Control Resp Yes Passes Criteria31.5 <<
0.13PMSD Yes Passes Criteria0.1456 0.47
ANOVA Table
Source Sum Squares Mean Square DF F Stat P-Value Decision(α:5%)
Between 5608.53 1121.71 5 55.91 <1.0E-05 Significant Effect
Error 1083.4 20.063 54
6691.93 59Total
ANOVA Assumptions Tests
Attribute Test Test Stat Critical P-Value Decision(α:1%)
15.11 15.09 0.0099 Unequal VariancesBartlett Equality of Variance TestVariance
0.9218 0.9459 0.0009 Non-Normal DistributionShapiro-Wilk W Normality TestDistribution
Mean Min MaxMedianCountCode Std ErrConc-% CV% %Effect95% LCL 95% UCL
Reproduction Summary
31.5 13 3832.50 10 2.227 22.36%26.46 36.54D 0.00%
32.9 27 373310 10 1.038 9.97%30.55 35.25 -4.44%
32.6 29 3732.6718 10 0.8589 8.33%30.66 34.54 -3.49%
32 29 3631.532 10 0.7601 7.51%30.28 33.72 -1.59%
27 21 3227.556 10 1.274 14.92%24.12 29.88 14.29%
5.8 0 165.5100 10 1.75 95.41%1.841 9.759 81.59%
CodeConc-%
Reproduction Detail
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
0 D 38 36 32 33 13 37 34 31 30 31
10 33 37 33 33 36 37 33 29 31 27
18 36 29 35 32 33 37 30 33 31 30
32 33 34 31 35 30 36 29 32 30 30
56 21 23 32 30 30 24 32 27 23 28
100 16 0 0 11 5 6 11 0 2 7
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 22 of 42 12/26/2024
Report Date: 20 Dec-24 09:12 (p 2 of 2)CETIS Analytical Report
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:08 Endpoint: Reproduction CETIS Version: CETISv2.1.4Analysis ID: 00-5155-7244
Analysis: Nonparametric-Control vs Treatments Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: B6805D4188EB3F0949F7DCC96921DE0D Editor ID: 005-847-680-2
Graphics
Conc-%
0 D 10 18 32 56 100
R ep ro d u ct io n
Rankits
2.01.51.00.50.0-0.5-1.0-1.5-2.0 U n tr an s fo rm e d
-5
-10
-15
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 23 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:12 (p 1 of 4)
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:07 Endpoint: 6d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 19-6819-5201
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: 9B9D24B8DF1F6A22FE98189F611BDCA8 Editor ID: 005-847-680-2
X Transform Y Transform Resamples Exp 95% CL
Linear Interpolation Options
MethodSeed
Linear Linear 1000 Yes Two-Point Interpolation1603763
Sample ID: 04-5552-4945
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 16-2952-0010
Start Date: 10 Dec-24 14:27
Ending Date: 16 Dec-24 13:54
Test Type: Reproduction-Survival (7d)
Test Length: 5d 23h
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Ceriodaphnia dubia
Source: In-House Culture
Analyst:
Age: <24Taxon: Branchiopoda
Point Estimates
Level 95% LCL 95% UCL% Tox Units 95% LCL 95% UCL
LC50 >100 --- --- <1 --- ---
Mean Min MaxCountCode ΣA/ΣB
Calculated Variate(A/B)
Conc-% CV% %Effect
6d Survival Rate Summary
Mean %Effect
Isotonic Variate
Median
1.0000 10/101.0000 1.00000 10D 0.00%0.00% 1.0000 0.00%1.0000
1.0000 10/101.0000 1.000010 10 0.00%0.00% 1.0000 0.00%1.0000
1.0000 10/101.0000 1.000018 10 0.00%0.00% 1.0000 0.00%1.0000
1.0000 10/101.0000 1.000032 10 0.00%0.00% 1.0000 0.00%1.0000
1.0000 10/101.0000 1.000056 10 0.00%0.00% 1.0000 0.00%1.0000
1.0000 10/101.0000 1.0000100 10 0.00%0.00% 1.0000 0.00%1.0000
CodeConc-%
6d Survival Rate Detail
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
0 D 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
10 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
18 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
32 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
56 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
100 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
CodeConc-%
6d Survival Rate Binomials
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
0 D 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
10 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
18 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
32 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
56 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
100 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 24 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:12 (p 2 of 4)
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:07 Endpoint: 6d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 19-6819-5201
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: 9B9D24B8DF1F6A22FE98189F611BDCA8 Editor ID: 005-847-680-2
Graphics
Conc-%
1009080706050403020100
6d S u rv iv al
R a te
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 25 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:12 (p 3 of 4)
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:07 Endpoint: Reproduction CETIS Version: CETISv2.1.4Analysis ID: 01-8584-7522
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: B6805D4188EB3F0949F7DCC96921DE0D Editor ID: 005-847-680-2
X Transform Y Transform Resamples Exp 95% CL
Linear Interpolation Options
MethodSeed
Linear Linear 1000 Yes Two-Point Interpolation772452
Attribute Test Stat Decision
Test Acceptability Criteria
Lower OverlapUpper
TAC Limits
15Control Resp Yes Passes Criteria31.5 <<
Sample ID: 04-5552-4945
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 16-2952-0010
Start Date: 10 Dec-24 14:27
Ending Date: 16 Dec-24 13:54
Test Type: Reproduction-Survival (7d)
Test Length: 5d 23h
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Ceriodaphnia dubia
Source: In-House Culture
Analyst:
Age: <24Taxon: Branchiopoda
Point Estimates
Level 95% LCL 95% UCL% Tox Units 95% LCL 95% UCL
IC25 61.71 54.88 66.01 1.6 1.5 1.8
Mean Min MaxCountCode
Calculated Variate
Conc-% CV% %Effect
Reproduction Summary
Mean %Effect
Isotonic Variate
Median
31.5 13 380 10D 0.00%22.36% 32.33 0.00%32.5
32.9 27 3710 10 -4.44%9.97% 32.33 0.00%33
32.6 29 3718 10 -3.49%8.33% 32.33 0.00%32.67
32 29 3632 10 -1.59%7.51% 32 1.02%31.5
27 21 3256 10 14.29%14.92% 27 16.49%27.5
5.8 0 16100 10 81.59%95.41% 5.8 82.06%5.5
CodeConc-%
Reproduction Detail
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
0 D 38 36 32 33 13 37 34 31 30 31
10 33 37 33 33 36 37 33 29 31 27
18 36 29 35 32 33 37 30 33 31 30
32 33 34 31 35 30 36 29 32 30 30
56 21 23 32 30 30 24 32 27 23 28
100 16 0 0 11 5 6 11 0 2 7
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 26 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:12 (p 4 of 4)
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:07 Endpoint: Reproduction CETIS Version: CETISv2.1.4Analysis ID: 01-8584-7522
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: B6805D4188EB3F0949F7DCC96921DE0D Editor ID: 005-847-680-2
Graphics
Conc-%
1009080706050403020100
R ep ro d u ct io n
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 27 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:13 (p 1 of 2)
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:07 Endpoint: 6d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 08-1067-3358
Analysis: STP 2xK Contingency Tables Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: 9B9D24B8DF1F6A22FE98189F611BDCA8 Editor ID: 005-847-680-2
Sample ID: 04-5552-4945
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 16-2952-0010
Start Date: 10 Dec-24 14:27
Ending Date: 16 Dec-24 13:54
Test Type: Reproduction-Survival (7d)
Test Length: 5d 23h
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Ceriodaphnia dubia
Source: In-House Culture
Analyst:
Age: <24Taxon: Branchiopoda
NOEL LOEL Tox UnitsTOELAlt HypData Transform
100 >100 1---C > TUntransformed
Test Stat P-Value Decision(α:5%)vsControl Conc-%
Fisher Exact/Bonferroni-Holm Test
P-Type
1.0000 1.0000 Non-Significant Effect10Dilution Water Exact
1.0000 1.0000 Non-Significant Effect18 Exact
1.0000 1.0000 Non-Significant Effect32 Exact
1.0000 1.0000 Non-Significant Effect56 Exact
1.0000 1.0000 Non-Significant Effect100 Exact
RNR NR + RCodeConc-% Prop NR Prop R %Effect
6d Survival Rate Frequencies
010 100 1.0000 0.0000 0.00%D
010 1010 1.0000 0.0000 0.00%
010 1018 1.0000 0.0000 0.00%
010 1032 1.0000 0.0000 0.00%
010 1056 1.0000 0.0000 0.00%
010 10100 1.0000 0.0000 0.00%
Mean Min MaxMedianCountCode Std ErrConc-% CV% %Effect95% LCL 95% UCL
6d Survival Rate Summary
1.0000 1.0000 1.00001.00000 10 0.0000 0.00%1.0000 1.0000D 0.00%
1.0000 1.0000 1.00001.000010 10 0.0000 0.00%1.0000 1.0000 0.00%
1.0000 1.0000 1.00001.000018 10 0.0000 0.00%1.0000 1.0000 0.00%
1.0000 1.0000 1.00001.000032 10 0.0000 0.00%1.0000 1.0000 0.00%
1.0000 1.0000 1.00001.000056 10 0.0000 0.00%1.0000 1.0000 0.00%
1.0000 1.0000 1.00001.0000100 10 0.0000 0.00%1.0000 1.0000 0.00%
CodeConc-%
6d Survival Rate Detail
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
0 D 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
10 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
18 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
32 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
56 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
100 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 28 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:13 (p 2 of 2)
Ceriodaphnia 7-d Survival and Reproduction Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:07 Endpoint: 6d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 08-1067-3358
Analysis: STP 2xK Contingency Tables Status Level: 1
Edit Date: 20 Dec-24 9:00 MD5 Hash: 9B9D24B8DF1F6A22FE98189F611BDCA8 Editor ID: 005-847-680-2
CodeConc-%
6d Survival Rate Binomials
Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10
0 D 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
10 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
18 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
32 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
56 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
100 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1
Graphics
Conc-%
0 D 10 18 32 56 100
6d S u rv iv a l R at e
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 29 of 42 12/26/2024
CETIS Summary Report Report Date: 20 Dec-24 09:20 (p 1 of 2)
Test Code/ID: 275382_FH / 16-6670-6228
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Batch ID: 03-5808-5145
Start Date: 10 Dec-24 14:38
Ending Date: 17 Dec-24 14:22
Test Type: Growth-Survival (7d)
Test Length: 7d
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Pimephales promelas
Source: Aquatox, AR
Analyst:
Age: <24Taxon: Actinopterygii
Sample ID: 03-0360-7599
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Multiple Comparison Summary
NOEL LOEL TOELEndpointAnalysis ID Comparison Method PMSD✓ STU
100 >100 ---15-6741-2524 16.8%7d Survival Rate 1Dunnett Multiple Comparison Test 1
100 >100 ---12-8233-3499 20.6%Mean Dry Biomass-mg 1Dunnett Multiple Comparison Test 1
95% LCL 95% UCL
Point Estimate Summary
EndpointAnalysis ID Point Estimate Method %Level✓ STU
LC50 >100 --- ---09-3057-6616 <1Linear Interpolation (ICPIN)7d Survival Rate 1
IC25 >100 --- ---11-7234-9997 <1Linear Interpolation (ICPIN)Mean Dry Biomass-mg 1
Test Acceptability
Attribute Test Stat DecisionAnalysis ID Endpoint Lower OverlapUpper
TAC Limits
0.92509-3057-6616 0.8Control Resp7d Survival Rate Yes Passes Criteria<<
0.92515-6741-2524 0.8Control Resp7d Survival Rate Yes Passes Criteria<<
0.876811-7234-9997 0.25Control RespMean Dry Biomass-mg Yes Passes Criteria<<
0.876812-8233-3499 0.25Control RespMean Dry Biomass-mg Yes Passes Criteria<<
0.206212-8233-3499 0.12PMSDMean Dry Biomass-mg Yes Passes Criteria0.3
Code Mean Min Max Std DevCount CV%Std ErrConc-%
7d Survival Rate Summary
95% LCL 95% UCL %Effect
0 0.9250 0.8000 1.0000 0.09574 10.35%0.04790.7727 1.0770 0.00%D
10 0.9750 0.9000 1.0000 0.05004 5.13%0.02500.8954 1.0550 -5.41%
18 0.9500 0.9000 1.0000 0.05774 6.08%0.02890.8581 1.0420 -2.70%
32 0.7750 0.7000 0.9000 0.09574 12.35%0.04790.6227 0.9273 16.22%
56 0.9250 0.8000 1.0000 0.09574 10.35%0.04790.7727 1.0770 0.00%
100 0.9000 0.8000 1.0000 0.11554 12.83%0.05770.7163 1.0840 2.70%
Code Mean Min Max Std DevCount CV%Std ErrConc-%
Mean Dry Biomass-mg Summary
95% LCL 95% UCL %Effect
0 0.8768 0.772 1.01 0.10274 11.72%0.051360.7133 1.04 0.00%D
10 0.9423 0.912 0.973 0.033814 3.59%0.01690.8885 0.996 -7.47%
18 0.9653 0.798 1.089 0.14324 14.84%0.071610.7374 1.193 -10.09%
32 0.8737 0.762 1.015 0.10624 12.16%0.053110.7047 1.043 0.34%
56 0.9995 0.845 1.058 0.10354 10.35%0.051740.8349 1.164 -14.00%
100 0.9445 0.782 1.058 0.11624 12.31%0.058120.7595 1.129 -7.73%
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 30 of 42 12/26/2024
CETIS Summary Report Report Date: 20 Dec-24 09:20 (p 2 of 2)
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
CodeConc-%
7d Survival Rate Detail
Rep 1 Rep 2 Rep 3 Rep 4
912BD8D95CC10354DF93A88D67663821MD5:
0 D 1.0000 1.0000 0.8000 0.9000
10 1.0000 1.0000 0.9000 1.0000
18 0.9000 0.9000 1.0000 1.0000
32 0.7000 0.7000 0.9000 0.8000
56 1.0000 0.8000 1.0000 0.9000
100 1.0000 0.8000 0.8000 1.0000
CodeConc-%
Mean Dry Biomass-mg Detail
Rep 1 Rep 2 Rep 3 Rep 4
2C4C422DAA2BD563CBE3470B6A7A1A07MD5:
0 D 1.01 0.898 0.772 0.827
10 0.97 0.912 0.914 0.973
18 0.894 0.798 1.089 1.08
32 0.837 0.881 1.015 0.762
56 1.058 0.845 1.037 1.058
100 0.974 0.964 0.782 1.058
CodeConc-%
7d Survival Rate Binomials
Rep 1 Rep 2 Rep 3 Rep 4
0 D 10/10 10/10 8/10 9/10
10 10/10 10/10 9/10 10/10
18 9/10 9/10 10/10 10/10
32 7/10 7/10 9/10 8/10
56 10/10 8/10 10/10 9/10
100 10/10 8/10 8/10 10/10
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 31 of 42 12/26/2024
Report Date: 20 Dec-24 09:20 (p 1 of 4)CETIS Analytical Report
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: 7d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 15-6741-2524
Analysis: Parametric-Control vs Treatments Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 912BD8D95CC10354DF93A88D67663821 Editor ID: 005-847-680-2
Sample ID: 03-0360-7599
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 03-5808-5145
Start Date: 10 Dec-24 14:38
Ending Date: 17 Dec-24 14:22
Test Type: Growth-Survival (7d)
Test Length: 7d
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Pimephales promelas
Source: Aquatox, AR
Analyst:
Age: <24Taxon: Actinopterygii
Test Stat Critical P-Value Decision(α:5%)MSDvsControl Conc-%
Dunnett Multiple Comparison Test
P-Typedf
-0.8161 2.407 0.2248 0.9720 Non-Significant Effect10Dilution Water 6 CDF
-0.3798 2.407 0.2248 0.9211 Non-Significant Effect18 6 CDF
2.253 2.407 0.2248 0.0666 Non-Significant Effect32 6 CDF
0 2.407 0.2248 0.8333 Non-Significant Effect56 6 CDF
0.3798 2.407 0.2248 0.6983 Non-Significant Effect100 6 CDF
NOEL LOEL Tox UnitsTOELAlt HypData Transform PMSDMSDu
100 >100 16.79%1---C > TAngular (Corrected) 0.1553
Attribute Test Stat Decision
Test Acceptability Criteria
Lower OverlapUpper
TAC Limits
0.8Control Resp Yes Passes Criteria0.925 <<
ANOVA Table
Source Sum Squares Mean Square DF F Stat P-Value Decision(α:5%)
Between 0.198417 0.0396835 5 2.275 0.0908 Non-Significant Effect
Error 0.314008 0.0174449 18
0.512425 23Total
ANOVA Assumptions Tests
Attribute Test Test Stat Critical P-Value Decision(α:1%)
2.108 15.09 0.8340 Equal VariancesBartlett Equality of Variance TestVariance
0.9192 0.884 0.0560 Normal DistributionShapiro-Wilk W Normality TestDistribution
Mean Min MaxMedianCountCode Std ErrConc-% CV% %Effect95% LCL 95% UCL
7d Survival Rate Summary
0.9250 0.8000 1.00000.96670 4 0.0479 10.35%0.7727 1.0000D 0.00%
0.9750 0.9000 1.00001.000010 4 0.0250 5.13%0.8954 1.0000 -5.41%
0.9500 0.9000 1.00000.950018 4 0.0289 6.08%0.8581 1.0000 -2.70%
0.7750 0.7000 0.90000.733332 4 0.0479 12.35%0.6227 0.9273 16.22%
0.9250 0.8000 1.00000.966756 4 0.0479 10.35%0.7727 1.0000 0.00%
0.9000 0.8000 1.00000.9000100 4 0.0577 12.83%0.7163 1.0000 2.70%
Mean Min MaxMedianCountCode Std ErrConc-% CV% %Effect95% LCL 95% UCL
Angular (Corrected) Transformed Summary
1.2950 1.1070 1.41201.35800 4 0.0735 11.35% 0.00%1.0610 1.5290D
1.3710 1.2490 1.41201.412010 4 0.0407 5.94% -5.89%1.2420 1.5010
1.3310 1.2490 1.41201.331018 4 0.0471 7.07% -2.74%1.1810 1.4800
1.0850 0.9912 1.24901.030032 4 0.0613 11.29% 16.25%0.8897 1.2800
1.2950 1.1070 1.41201.358056 4 0.0735 11.35% 0.00%1.0610 1.5290
1.2600 1.1070 1.41201.2600100 4 0.0880 13.97% 2.74%0.9795 1.5400
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 32 of 42 12/26/2024
Report Date: 20 Dec-24 09:20 (p 2 of 4)CETIS Analytical Report
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: 7d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 15-6741-2524
Analysis: Parametric-Control vs Treatments Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 912BD8D95CC10354DF93A88D67663821 Editor ID: 005-847-680-2
CodeConc-%
7d Survival Rate Detail
Rep 1 Rep 2 Rep 3 Rep 4
0 D 1.0000 1.0000 0.8000 0.9000
10 1.0000 1.0000 0.9000 1.0000
18 0.9000 0.9000 1.0000 1.0000
32 0.7000 0.7000 0.9000 0.8000
56 1.0000 0.8000 1.0000 0.9000
100 1.0000 0.8000 0.8000 1.0000
CodeConc-%
Angular (Corrected) Transformed Detail
Rep 1 Rep 2 Rep 3 Rep 4
0 D 1.4120 1.4120 1.1070 1.2490
10 1.4120 1.4120 1.2490 1.4120
18 1.2490 1.2490 1.4120 1.4120
32 0.9912 0.9912 1.2490 1.1070
56 1.4120 1.1070 1.4120 1.2490
100 1.4120 1.1070 1.1070 1.4120
CodeConc-%
7d Survival Rate Binomials
Rep 1 Rep 2 Rep 3 Rep 4
0 D 10/10 10/10 8/10 9/10
10 10/10 10/10 9/10 10/10
18 9/10 9/10 10/10 10/10
32 7/10 7/10 9/10 8/10
56 10/10 8/10 10/10 9/10
100 10/10 8/10 8/10 10/10
Graphics
Conc-%
0 D 10 18 32 56 100
7d S u rv iv al
R a te
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
Rankits
1.51.00.50.0-0.5-1.0-1.5
C o rr . A n g le
0.20
0.15
0.10
0.05
0.00
-0.05
-0.10
-0.15
-0.20
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 33 of 42 12/26/2024
Report Date: 20 Dec-24 09:20 (p 3 of 4)CETIS Analytical Report
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: Mean Dry Biomass-mg CETIS Version: CETISv2.1.4Analysis ID: 12-8233-3499
Analysis: Parametric-Control vs Treatments Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 2C4C422DAA2BD563CBE3470B6A7A1A0 Editor ID: 005-847-680-2
Sample ID: 03-0360-7599
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 03-5808-5145
Start Date: 10 Dec-24 14:38
Ending Date: 17 Dec-24 14:22
Test Type: Growth-Survival (7d)
Test Length: 7d
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Pimephales promelas
Source: Aquatox, AR
Analyst:
Age: <24Taxon: Actinopterygii
Test Stat Critical P-Value Decision(α:5%)MSDvsControl Conc-%
Dunnett Multiple Comparison Test
P-Typedf
-0.872 2.407 0.1808 0.9757 Non-Significant Effect10Dilution Water 6 CDF
-1.178 2.407 0.1808 0.9895 Non-Significant Effect18 6 CDF
0.03996 2.407 0.1808 0.8213 Non-Significant Effect32 6 CDF
-1.634 2.407 0.1808 0.9972 Non-Significant Effect56 6 CDF
-0.902 2.407 0.1808 0.9776 Non-Significant Effect100 6 CDF
NOEL LOEL Tox UnitsTOELAlt HypData Transform PMSDMSDu
100 >100 20.62%1---C > TUntransformed 0.1808
Attribute Test Stat Decision
Test Acceptability Criteria
Lower OverlapUpper
TAC Limits
0.25Control Resp Yes Passes Criteria0.8768 <<
0.12PMSD Yes Passes Criteria0.2062 0.3
ANOVA Table
Source Sum Squares Mean Square DF F Stat P-Value Decision(α:5%)
Between 0.0494089 0.0098818 5 0.8757 0.5168 Non-Significant Effect
Error 0.203115 0.0112842 18
0.252524 23Total
ANOVA Assumptions Tests
Attribute Test Test Stat Critical P-Value Decision(α:1%)
4.334 15.09 0.5024 Equal VariancesBartlett Equality of Variance TestVariance
0.9466 0.884 0.2286 Normal DistributionShapiro-Wilk W Normality TestDistribution
Mean Min MaxMedianCountCode Std ErrConc-% CV% %Effect95% LCL 95% UCL
Mean Dry Biomass-mg Summary
0.8768 0.772 1.010.86250 4 0.05136 11.72%0.7133 1.04D 0.00%
0.9423 0.912 0.9730.94210 4 0.0169 3.59%0.8885 0.996 -7.47%
0.9653 0.798 1.0890.98718 4 0.07161 14.84%0.7374 1.193 -10.09%
0.8737 0.762 1.0150.85932 4 0.05311 12.16%0.7047 1.043 0.34%
0.9995 0.845 1.0581.04756 4 0.05174 10.35%0.8349 1.164 -14.00%
0.9445 0.782 1.0580.969100 4 0.05812 12.31%0.7595 1.129 -7.73%
CodeConc-%
Mean Dry Biomass-mg Detail
Rep 1 Rep 2 Rep 3 Rep 4
0 D 1.01 0.898 0.772 0.827
10 0.97 0.912 0.914 0.973
18 0.894 0.798 1.089 1.08
32 0.837 0.881 1.015 0.762
56 1.058 0.845 1.037 1.058
100 0.974 0.964 0.782 1.058
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 34 of 42 12/26/2024
Report Date: 20 Dec-24 09:20 (p 4 of 4)CETIS Analytical Report
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: Mean Dry Biomass-mg CETIS Version: CETISv2.1.4Analysis ID: 12-8233-3499
Analysis: Parametric-Control vs Treatments Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 2C4C422DAA2BD563CBE3470B6A7A1A0 Editor ID: 005-847-680-2
Graphics
Conc-%
0 D 10 18 32 56 100
M e a n
D ry
B io m a ss -m g
1.0
0.8
0.6
0.4
0.2
0.0
Rankits
1.51.00.50.0-0.5-1.0-1.5 U n tr an s fo rm e d
0.15
0.10
0.05
0.00
-0.05
-0.10
-0.15
CETIS™ v2.1.4.8 x64 (005-847-680-2)Convergent Rounding (4 sf) QA:________Analyst:________
Page 35 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:20 (p 1 of 4)
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: 7d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 09-3057-6616
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 912BD8D95CC10354DF93A88D67663821 Editor ID: 005-847-680-2
X Transform Y Transform Resamples Exp 95% CL
Linear Interpolation Options
MethodSeed
Linear Linear 1000 Yes Two-Point Interpolation1903839
Attribute Test Stat Decision
Test Acceptability Criteria
Lower OverlapUpper
TAC Limits
0.8Control Resp Yes Passes Criteria0.925 <<
Sample ID: 03-0360-7599
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 03-5808-5145
Start Date: 10 Dec-24 14:38
Ending Date: 17 Dec-24 14:22
Test Type: Growth-Survival (7d)
Test Length: 7d
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Pimephales promelas
Source: Aquatox, AR
Analyst:
Age: <24Taxon: Actinopterygii
Point Estimates
Level 95% LCL 95% UCL% Tox Units 95% LCL 95% UCL
LC50 >100 --- --- <1 --- ---
Mean Min MaxCountCode ΣA/ΣB
Calculated Variate(A/B)
Conc-% CV% %Effect
7d Survival Rate Summary
Mean %Effect
Isotonic Variate
Median
0.9250 37/400.8000 1.00000 4D 0.00%10.35% 0.9500 0.00%0.9667
0.9750 39/400.9000 1.000010 4 -5.41%5.13% 0.9500 0.00%1.0000
0.9500 38/400.9000 1.000018 4 -2.70%6.08% 0.9500 0.00%0.9500
0.7750 31/400.7000 0.900032 4 16.22%12.35% 0.8667 8.77%0.7333
0.9250 37/400.8000 1.000056 4 0.00%10.35% 0.8667 8.77%0.9667
0.9000 36/400.8000 1.0000100 4 2.70%12.83% 0.8667 8.77%0.9000
CodeConc-%
7d Survival Rate Detail
Rep 1 Rep 2 Rep 3 Rep 4
0 D 1.0000 1.0000 0.8000 0.9000
10 1.0000 1.0000 0.9000 1.0000
18 0.9000 0.9000 1.0000 1.0000
32 0.7000 0.7000 0.9000 0.8000
56 1.0000 0.8000 1.0000 0.9000
100 1.0000 0.8000 0.8000 1.0000
CodeConc-%
7d Survival Rate Binomials
Rep 1 Rep 2 Rep 3 Rep 4
0 D 10/10 10/10 8/10 9/10
10 10/10 10/10 9/10 10/10
18 9/10 9/10 10/10 10/10
32 7/10 7/10 9/10 8/10
56 10/10 8/10 10/10 9/10
100 10/10 8/10 8/10 10/10
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 36 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:20 (p 2 of 4)
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: 7d Survival Rate CETIS Version: CETISv2.1.4Analysis ID: 09-3057-6616
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 912BD8D95CC10354DF93A88D67663821 Editor ID: 005-847-680-2
Graphics
Conc-%
1009080706050403020100
7d S u rv iv al
R a te
0.96
0.80
0.64
0.48
0.32
0.16
0.00
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 37 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:20 (p 3 of 4)
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: Mean Dry Biomass-mg CETIS Version: CETISv2.1.4Analysis ID: 11-7234-9997
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 2C4C422DAA2BD563CBE3470B6A7A1A0 Editor ID: 005-847-680-2
X Transform Y Transform Resamples Exp 95% CL
Linear Interpolation Options
MethodSeed
Linear Linear 1000 Yes Two-Point Interpolation712031
Attribute Test Stat Decision
Test Acceptability Criteria
Lower OverlapUpper
TAC Limits
0.25Control Resp Yes Passes Criteria0.8768 <<
Sample ID: 03-0360-7599
Sample Date: 09 Dec-24 11:10
Receipt Date: 10 Dec-24 08:55
Code: 275382
Sample Age: 27h
Source: ECC
Station: OF-EFF-120924
Client:
Project:
Material: POTW Effluent
CAS (PC):
Batch ID: 03-5808-5145
Start Date: 10 Dec-24 14:38
Ending Date: 17 Dec-24 14:22
Test Type: Growth-Survival (7d)
Test Length: 7d
Protocol: EPA/821/R-02-013 (2002) Diluent: Mod-Hard Synthetic Water
Brine:Species: Pimephales promelas
Source: Aquatox, AR
Analyst:
Age: <24Taxon: Actinopterygii
Point Estimates
Level 95% LCL 95% UCL% Tox Units 95% LCL 95% UCL
IC25 >100 --- --- <1 --- ---
Mean Min MaxCountCode
Calculated Variate
Conc-% CV% %Effect
Mean Dry Biomass-mg Summary
Mean %Effect
Isotonic Variate
Median
0.8768 0.772 1.010 4D 0.00%11.72% 0.9337 0.00%0.8625
0.9423 0.912 0.97310 4 -7.47%3.59% 0.9337 0.00%0.942
0.9653 0.798 1.08918 4 -10.09%14.84% 0.9337 0.00%0.987
0.8737 0.762 1.01532 4 0.34%12.16% 0.9337 0.00%0.859
0.9995 0.845 1.05856 4 -14.00%10.35% 0.9337 0.00%1.047
0.9445 0.782 1.058100 4 -7.73%12.31% 0.9337 0.00%0.969
CodeConc-%
Mean Dry Biomass-mg Detail
Rep 1 Rep 2 Rep 3 Rep 4
0 D 1.01 0.898 0.772 0.827
10 0.97 0.912 0.914 0.973
18 0.894 0.798 1.089 1.08
32 0.837 0.881 1.015 0.762
56 1.058 0.845 1.037 1.058
100 0.974 0.964 0.782 1.058
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 38 of 42 12/26/2024
CETIS Analytical Report Report Date: 20 Dec-24 09:20 (p 4 of 4)
Fathead Minnow 7-d Larval Survival and Growth Test Eurofins Arkansas
Analyzed: 20 Dec-24 9:19 Endpoint: Mean Dry Biomass-mg CETIS Version: CETISv2.1.4Analysis ID: 11-7234-9997
Analysis: Linear Interpolation (ICPIN) Status Level: 1
Edit Date: 20 Dec-24 9:15 MD5 Hash: 2C4C422DAA2BD563CBE3470B6A7A1A0 Editor ID: 005-847-680-2
Graphics
Conc-%
1009080706050403020100
M e a n
D ry
B io m a ss -m g
0.96
0.80
0.64
0.48
0.32
0.16
0.00
CETIS™ v2.1.4.8 x64 (005-847-680-2) QA:________Analyst:________Convergent Rounding (4 sf)
Page 39 of 42 12/26/2024
Page 40 of 42 12/26/2024
Page 41 of 42 12/26/2024
Page 42 of 42 12/26/2024
77th Quarter Final Report Old O-Field GWTF Quarterly Effluent Toxicity Study
ATTACHMENT 2
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| ��� ��� �� | ��,N�K'O�� � | K� | ||
| K | � (�I�"����V��U��� ��� , RS�� | !���� | ||
| ��� ��� �� | � | �N�K'O | � � | K� |
| K | � (�I�"������"��� ��� , RS�� !���� | |||
| � | � ��� �� �� N�K'O�� � | K� | ||
| K | � (�I�"�������� ��� , RS��� !���� | |||
| ��� ��� ��� �� N�K'O�� � | K� |
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