Attachment 4 - Mold Survey Results Letter - 2 December 2025.pdf

DEPARTMENT OF THE AIR FORCE

UNITED STATES SPACE FORCE

SPACE LAUNCH DELTA 45

2 December 2025 MEMORANDUM FOR First Air Force, Detachment 3

FROM: 45 OMRS/SGXB

SUBJECT: Mold Survey Results for Bldg. 559

BLUF: A comprehensive fungal analysis of Bldg. 559, conducted by NASA Environmental and Medical Contract (NEMCON), found indoor mold levels to be within acceptable parameters.

1. BACKROUND: NEMCON conducted mold sampling in Bldg. 559 to determine if any areas exceeded recommended comfort levels. While no regulatory limits exist, indoor levels exceeding ten times outdoor levels would be a cause for concern. The goal of this assessment is to support effective health risk management and help provide a work environment that meets recommended comfort parameters.

2. ASSESSMENT: NEMCON surveyed the building using a Zefon Bio-Pump Plus (ZBP-205, Serial 07988), calibrated to 15 LPM, to assess and quantify the total concentration and speciation of airborne fungal spores. This sampling method detects both live and dead mold spores, providing a comprehensive analysis of fungal presence. Multiple rooms throughout Building 559 were sampled.

3. RESULTS: Indoor mold levels were generally lower than outdoor levels, as expected. While some variation existed between rooms, none of the sampled areas had indoor levels exceeding ten times outdoor levels. See Attachment 1 for the complete laboratory analysis. A separate Agar Plate test has also been conducted; those results are expected in 2-3 weeks and will be provided in a separate memorandum.

4. INFO: Mold is common and tends to proliferate in warm, humid environments. Individual responses to mold exposure vary; some individuals sensitive to mold may experience allergy-like symptoms, while those with weakened immune systems are more susceptible to respiratory issues.

5. We look forward to working with you to ensure workers are safe and healthy while completing the mission. Copies of this report and applicable supporting documentation are available for your review in our office, building 1366, during business hours. If you have any questions or concerns about this report, please contact BE at (321) 494-5435 (DSN 854) or usaf.patrick.45sw-mdg.mbx.bioenvironmental-engineering-flight@health.mil.

OCTAEVIA R. HUTCHERSON, TSgt, USAF NCOIC, Occ Hlth/Ind Hyg Ops

Attachment:

1. KSC Environmental Microbiology Laboratory Results

KSC ENVIRONMENTAL MICROBIOLOGY LABORATORY

Bldg. M7-0355 Rm. 2272 (Mail Code: NEM-004)

Kennedy Space Center, FL 32899

AIHA EMPAT #180018, Phone: (321)-867-7471

Test Report: Air-O-Cell Analysis of Fungal Spores & Particulates by Optical Microscopy

Lab Sample Number E251243 E251244 E251245 E251246 E251247

Client ID: AoC-1 AoC-2 AoC-3 AoC-4 AoC-5

Flow Rate & Sample Time: 15L/min for 10 minutes 15L/min for 10 minutes 15L/min for 10minutes 15L/min for 10minutes 15L/min for 10 mins

Total Volume Sampled (L): 150L 150L 150L 150L 150L

Sample Location: Conference Room 100 RM 201 (Comparison 1) RM 208 (Comparison 2) RM 101 CSS Office Outdoor Sample

Temperature:

Relative Humidity:

Temp – 69.6˚F

RH – 58%

Temp – 63.9˚F

RH – 63%

Temp – 65.5˚F

RH - 58%

Temp – 69.6˚F

RH – 54%

Temp – 74.1˚F

RH – 52%

Spore Types Raw

Count Count/m3

Raw Count

Count/m3 Raw

Count Count/m3

Raw Count

Count/m3 Raw

Count Count/m3

Alternaria - - - - - - - - 1 6.7 Ascospores 3 20.0 4 26.7 2 13.3 1 6.7 11 73.3

Aspergillus/Penicillium 10 66.7 13 86.7 33 220.0 4 26.7 21 140.0 Basidiospores 6 40.0 1 6.7 - - - - - -

Bipolaris - - - - - - - - - - Cercospora - - - - - - - - - -

Chaetomium - - - - - - - - - - Cladosporium - - 6 40.0 5 33.3 2 13.3 72 480.0

Curvularia 2 13.3 1 6.7 - - 1 6.7 2 13.3 Epicoccum - - - - - - 1 6.7 - -

Fusarium - - - - - - - - 3 20.0 Myxomycetes 1 6.7 - - 1 6.7 - - 2 13.3

Pithomyces - - 1 6.7 - - - - - - Rust - - - - - - - - - -

Scopulariopsis - - - - - - - - - - Stachybotrys - - - - - - - - - -

Torula - - - - - - - - - - Ulocladium - - - - - - - - - -

Unidentifiable Spores - - - - - - - - - - Zygophilia - - - - - - - - - -

Total Fungi 22 146.7 26 173.3 41 273.3 9 60.0 112 746.7

Other Particulates

Skin Fragment (1-4) ( 2 ) Low ( 2 ) Low ( 2 ) Low ( 2 ) Low ( 1 ) Very Low

Hyphal Fragments (1-4) ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low

Insect Fragments (1-4) ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low

Fibrous Particulate (1-4) ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low ( 1 ) Very Low

Background (1-5) ( 2 ) Low ( 2 ) Low ( 2 ) Low ( 2 ) Low ( 2 ) Low

Field Notes:

• Air samples were collected using a Zefon Bio-Pump Plus (ZBP-205, Serial 07988) verified at 15/LPM.

• (AoC-4) RM 101 windows were open upon arrival and closed about an hour prior to sampling.

High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of

5 indicate an overloading of background particulates, prohibiting accurate detection and quantification.

(*Present) = Spores were observed but could not be enumerated due to high background interference.

Identifications were performed at a magnification of 400X. Due to method stopping rules, raw counts more than 200 are extrapolated based on the percentage of trace analyzed. Results are not blank corrected unless otherwise noted.

The minimum detection limit is equal to one fungal spore. Counts/m3 were calculated based on the total volume sampled. Results were rounded to the nearest 0.1 cfu/m3.

( -- ) = Spore Type was not observed in the sample. Not Detected.

KSCEML maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not be reproduced, except in full, without written approval. KSCEML bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the responsibility of the client. Samples were received in good condition unless otherwise noted.

11/19/2025

X Jason Johns

Jason Johns

Environmental Microbiologist

Signed by: Jason Johns (affiliate)

Attn: TSgt. Octaevia Hutcherson / PSFB Phone: NA

USAF DHA PATRICK 45TH MDG (USA)

Patrick Space Force Base, FL 32925 Collected: 11/14/2025

Email: octaevia.r.hutcherson.mil@health.mil Received: 11/14/2025

Project: PSFB Building 559 IAQ Survey (Air-O-Cell) Analyzed: 11/14 & 11/17

NEMCON Task #: T202511-64543

Lab ID #: 25-318

Report Date: 11/19/2025

Report #: EML-25-229

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