Attachment 1 - Purchase Description.pdf

Purchase Description

1.0 TITLE

Procurement of One (1) Zeiss Lattice SIM 5 laser scanning structured illumination super-resolution light microscope and Associated Supplies

2.0 BACKGROUND

The U.S. Department of Health and Human Services (HHS), National Institutes of Health (NIH), National Cancer Institute (NCI) Center for Cancer Research (CCR), Laboratory of Cancer Biology and Genetics (LCBG), CCR Microscopy Core requires one (1) new, commercially available, Zeiss Lattice SIM5 super-resolution light microscope.

The National Cancer Institute is the principal federal agency for cancer research and training. It leads, conducts, and supports cancer research to advance scientific knowledge and improve cancer prevention, detection, diagnosis, and survivorship. The Center for Cancer Research (CCR) is the largest division of the NCI’s intramural research program with both basic and clinical research groups exploring the cutting edge of cancer research involving a wide spectrum of biological and biomedical problems that range from visualizing and understanding the structure of genes and proteins and developing novel assays to detect and potentially treat patients. The CCR Microscopy Core, the main microscopy core in the CCR, serves the imaging needs of a wide range of NCI researchers studying the cell biology of cancer. The core facility has five high-quality research-grade instruments, including confocal, multi-photon and super-resolution microscopes, to apply to the numerous research projects from the over 45 labs/branches within NCI, that use the Core annually.

To satisfy the demands for super-resolution fluorescence microscopy, the CCR Microscopy Core requests a new Zeiss Lattice SIM5 structured illumination super-resolution microscope. Structured illumination super-resolution microscopy is an established method for acquiring high resolution images of biological samples and is in high demand from NCI researchers. The demand arises from the need to understand the molecular interactions of cellular proteins and the gene expression profiles of tumor cells. Super-resolution microscopy provides researchers the ability to visualize these molecular interactions within cells, and the newest SIM technology available from the requested Zeiss Latice SIM5 microscope will allow researchers to image these interactions in live cells. Thus, providing this cutting-edge technology to NCI researchers to apply to their cancer research projects and further improve our understanding of the cell biology of cancer.

The CCR Microscopy Core requires a new Zeiss Lattice SIM5 super-resolution microscope that shall include a high-speed lattice SIM5 scanner, dual high-sensitivity sCMOS cameras with Duolink configuration for simultaneous 2 channel imaging, four high-power lasers (wavelengths 405, 488, 561 and 647 nm), 500 um Piezo stage, stage-top incubation for live cell imaging, and both high numerical aperture oil and water immersion objective lenses for super-resolution imaging. Thus, it provides extended capabilities to image complex samples but also has the capability to perform functional live cell SIM imaging on larger biological samples. All users of the CCR Microscopy

Core interested in performing SIM super-resolution microscopy will benefit from the requested Zeiss Lattice SIM5 microscope.

3.0 TYPE OF ORDER

This is a Firm Fixed-Price Purchase Order.

4.0 SPECIAL ORDER REQUIREMENTS

The Contractor shall provide the following items “brand name” or “brand name or equal”:

Item Product Description Product/Part No. Quantity 1 Microscope Lattice SIM 3/5 493505-9880-010 1 2 Axio Observer 7 SR f/Lattice SIM 409000-9810-000 1 3 Autoimmersion module w/pipette 433801-9040-000 1 4 Condenser 0,55 mot f/SF 428400-9000-000 1

Calibration target f/SF stage incubator Calibration plate 128x86 for AI Sample Finder for Lattice

SIM

428400-9210-000 1

Joystick XY; CAN for mechanical stages mot; CAN, scanning stages; CAN and stage controller XY STEP SMC 2009

432903-9012-000 1

9 Extension cable for stage control 1.6 m 000000-2121-749 1 10 Stage attachment Z PIEZO WSB 500 432339-9000-000 1 11 Controller Z PIEZO WSB 500 432339-9010-000 1 12 Control wheel Z PIEZO WSB 500 432339-9020-000 1

13 Stage Top incubation heating for Lattice SIM inverted includes heating insert, heating cover and controller 000000-2519-303 1

14 Stage top incubator gas system for Lattice SIM inverted 000000-2570-331 1

15 Universal frame insert for stage incubation Lattice SIM suitable for cell culture dishes, slides and chamber slides 000000-2623-209 1

16 Holder frame f/slides 000000-2519-300 1 17 Holder frame f/chamber slide 000000-2519-301 1 18 Petri dish holding frame D35-40mm 000000-2519-299 1

19 Hold-down for petri dishes in addition to holding frame petri dish D35-40 mm 000000-2519-302 1

21 Halogen lamp 12V 100W GY6.35 380059-1660-000 2 23 FL Filter Set 77 HE GFP/mRFP/Alexa 633 489077-0000-000 1 26 Illumination module f/Lattice SIM 5 000000-2592-313 1 27 Reflectorrev Obs w/filter f/Lattice SIM 000000-2507-215 1 28 Camera ORCA Fusion BT w/adapter 000000-2519-289 1

Camera accessories for ORCA Fusion BT includes framegrabber card, liquid cooling systen, coolant and mains cable

000000-2519-290 1

Laser module URGB / 50mW Laser module LM URGB for Lattice SIM with fiber coupled, pigtailed and colimated lasers.

Following lasers are available:

- diode laser 405nm, 50 mW, laser class 3B

- diode laser 488nm, 50 mW, laser class 3B

- diode laser (SHG) 561nm, 50 mW, laser class 3B

- diode laser 647nm, 50 mW, laser class 3B

000000-2520-363 1

31 ECU desktop housing HR f/microscopes 000000-2183-235 1 32 slide-in module ECU Power 12V/60W 000000-2595-493 1

33 Rack for Lattice SIM for laser module (LM) and electronic control unit (ECU) 000000-2513-762 1

34 Dual camera adapter DuoLink SR 000000-2579-174 1 35 Camera ORCA Fusion BT w/adapter 000000-2519-289 1 36 Filtermodule DuoLink SR QUAD Lattice SIM 000000-2502-309 1 37 Multispectral probe f/Airyscan 100 nm 000000-2114-035 1 38 Multicolor calibration slide 000000-2076-515 1 40 Objective C-Apo 40x/1.2 W Corr 421767-9973-799 1 42 Objective PApo 40x/1.4 Oil DIC (UV) V-IR 420762-9700-000 1 43 Plan-Apochromat 63x/1.40 Oil DIC f/ELYRA 420782-9900-799 1 44 Objective LD LCI PApo 25x/0.8 Imm Corr D 420852-9872-799 1 6 Scanning Stage 130x100 432029-9904-000 1 7 Stepper motor control f. 2 Axes SMC2009 432929-9011-000 1 20 Lamphousing 12V/100W w/ Collector 423000-9901-000 1 22 Illuminator HXP 120V 423013-9010-000 1 24 Reflector Module FL EC ACR P&C 424933-0000-000 1 41 Objective PApo 20x/0.8 420650-9903-000 1 45 EC Plan-Neofluar 10x/0.30 WD=5.2 M27 420340-9901-000 1 46 Objective ring ACR f/object sleeve cyl 424508-0000-000 1 47 Objective Ring ACR f/ obj. casing, short 424509-0000-000 2 48 Objective Ring ACR f/ obj. casing, long 424510-0000-000 2 49 Objective Ring ACR f/ obj. casing, long 424511-0000-000 1

System table , small, air damped, level regulated pressured air supply necessary, long and short side open, with breadboard, 900x750mm

000000-2147-374 1

25 ZEISS Predictive Service Remote Monitor 000000-2386-916 1 50 Microscopy Workstation Premium (HPZ8G5) 410203-9914-000 1 52 Processor Intel Xeon W5-3425 (HPZ8G5) 410330-1100-000 1 53 Memory 64GB (1x64) DDR5 4800 (HPZ8G5) 410303-6401-000 4

54 Graphics Card NVIDIA RTX A4000 16 GB DP 410331-2800-000 1 55 Network Adapter 2 x 10GbE RJ45 (HPZ8G5) 410358-0505-000 1 56 Real Time Controller Imaging Systems 400100-8630-000 1

57 Language Pack English US (O)

- Keyboard with English (US) layout 410380-0200-000 1

58 Monitor TFT 31,5" HP S7 Pro 732pk 410350-3205-000 1 60 SysConf2b HPZ8 G5 Win11 LTSC 2024 System 000000-2677-269 1 51 SysConf2b HPZ8 G5 Win11 LTSC 2024 System 000000-2677-269 1 59 Microscopy Workstation Premium (HPZ8G5) 410203-9914-000 1 61 Processor Intel Xeon W7-3555 (HPZ8G5) 410330-1300-000 1 62 Memory 64GB (1x64) DDR5 4800 (HPZ8G5) 410303-6401-000 4 63 Graphics Card NVIDIA RTX A5000 24 GB DP 410331-2900-000 1 64 Network Adapter 2 x 10GbE RJ45 (HPZ8G5) 410358-0505-000 1 65 HDD Extension to 20TB RAID10 (HPZ8G5) 410324-0020-000 1

66 Language Pack English US (O)

- Keyboard with English (US) layout 410380-0200-000 1

67 Monitor TFT 31,5" HP S7 Pro 732pk 410350-3205-000 1 68 SW ZEN 3.13 DLic 410135-0002-313 1 69 SW ZEN Toolkit Adv Acquisition 3.13 DLic 410136-0166-313 1 70 SW ZEN Toolkit Smart Acquisition DLic 410136-0167-312 1 71 SW ZEN Toolkit 2D DLic 410136-0168-360 1 72 SW ZEN Toolkit 3D DLic 410136-0172-360 1 73 SW ZEN Driver Hamamatsu Camera DLic 410136-0045-370 1 74 SW ZEN Module Lattice SIM DLic 410136-0133-390 1 75 SMA Fee ZEN w/Advanced Acq 1 year 410139-0166-000 1 76 SMA Fee ZEN Band C 1 year 410139-5003-000 1 77 SW ZEN 3.13 desk DLic 410135-0004-313 1 78 SW ZEN desk Toolkit 3D DLic 410137-0172-370 1 79 SW ZEN desk Module Lattice SIM DLic 410137-0133-390 1 80 SMA Fee ZEN desk 1 year 410139-0004-000 1 81 SMA Fee ZEN Band A 1 year 410139-5001-000

Additional Requirements

Must include a minimum of 12-month manufacturer’s warranty and a minimum of _12_-month maintenance support services as a part of the manufacturer’s warranty, to include all labor, material, and equipment necessary to repair or replace the system. Support service shall be available Monday – Friday 8am – 5pm EST, excluding Federal Holidays.

4.1 PRODUCT FEATURES/SALIENT CHARACTERISTICS

The following product features/characteristics are needed for this requirement:

1. Zeiss Lattice SIM5 illumination module for super-resolution imaging

a. Includes SIM Apotome and extended Lattice SIM with central power supply

b. Reflector Turret Observer with filter set FLEX for Lattice SIM

c. Four filter cubes adjusted for super-resolution microscopy; 2 positions for Push & Click filters

d. Laser module LM URGB for Lattice SIM with fiber coupling, pigtailed and collimated lasers, including laser lines405, 488, 561, and 647 nm.

e. DuoLink adapter for Lattice SIM to connect two sCMOS cameras of the same type

f. Filtermodule DuoLink SR Quad for Lattice SIM

2. System Air Table to accommodate microscope and SIM illumination module and provide vibration control

a. A 900 x 700mm breadboard with air dampening and level regulated

3. Hamamatsu ORCA Fusion BT sCMOS cameras for Lattice SIM to acquire fluorescence super-resolution images :

a. QE up to 95%, sensor pixel size 6.5 um, and number of pixels in sensor at 2304 x

b. Camera adapters for ORCA Fusion BT camera

4. Zeiss AxioObserver 7 Inverted Microscope for super-resolution Lattice SIM configured with

a. Scanning stage 130x100

b. Stepper motor control SMC2009

c. Joystick stage controller

d. Port adapter for Lattice SIM illumination module

e. TFT touchscreen with docking station

f. Z Piezo WSB 500 with controller

5. Multiple objective lenses for structured illumination microscopy to accommodate a range of biological samples

a. Plan-apochromat 20x N.A. 0.8 lens

b. EC Plan-neofluar 10x N.A. 0.3 lens

c. C-apochromat 40x N.A. 1.2 water immersion lens

d. Plan-apochromat 40x N.A. 1.4 oil immersion lens

e. Plan-apochromat 63x N.A. 1.40 oil immersion lens

f. LD LCI Plan-apochromat 25x N.A. 0.8 multi-immersion lens

6. Stage top incubation system to control temperature, humidity and CO2 for live cell imaging

a. Stage top incubation heating system for Lattice SIM, including heating insert, heating cover and controller

b. Stage top incubator gas system for Lattice SIM

7. Premium microscopy workstation for advanced offline image processing

a. Minimum 256 GB RAM memory

b. Minimum 24 GB graphics card

4.2 DELIVERY / INSTALLATION

Note: All shipping/handling (including FOB) and delivery/installation fees shall be included in the quote. The NCI will not be responsible in the event charges are not included in the quote.

The equipment shall be delivered within six months of award to:

NIH/NCI

Bldg. 37, Rm. B114A 37 Convent Drive Bethesda, MD 20892

The Contractor shall provide the NCI Technical Point of Contact (TPOC) with tracking information to anticipate delivery to the best extend possible. Upon delivery, the Contractor shall notify the TPOC, to schedule installation dates and times. Installation shall occur within two (2) weeks of delivery and shall be performed by or under the direct supervision of an OEM-authorized and certified technician.

Technical Point of Contact The Technical Point of Contact (TPOC) is responsible for inspection and acceptance of all deliverables in relation to this requirement and will serve as the Governments primary point of contact throughout the duration of the requirement. All efforts must be coordinated with this individual. The TPOC for this requirement is: TBA

Note: The TPOC has limited authority as defined within this Statement of Need. They do not have the authority to commit or obligate Government funds, or make changes to the terms and conditions of the purchase order. As such, any changes to the purchase order must be performed by a warranted Government Contracting Officer with appropriate delegated authority to do so.

4.3 TRAINING

The Contractor shall provide in-person training for up to 10 user(s). All training expenses, including materials and or travel expenses, shall be included in the quoted price. Training shall be conducted by a factory-trained product specialist and shall take place within 30 business day(s) of installation.

5.0 WARRANTY

The Contractor shall warrant all supplies furnished under this award against any defects in design, material, or manufacture for a period of at least twelve (12) months from date of final installation of all equipment and associated items provided under, or as part of this award. The warranty shall not exclude or limit the Governments rights for an Implied warranty as established in the terms and conditions of clause 52.212-4. The Contractor shall also warrant and imply that all items delivered as a result of this order are merchantable and fit for use for the particular purpose described in the order in accordance with FAR 52.212-4(o).

If a defect results in lost or decreased performance of supplies or services furnished under this order, the Contractor shall cover all parts, materials, labor, and/or travel expenses required to repair or replace the supplies at no additional cost to the Government. Supplies which are repaired or replaced shall be warranted for the remainder of the initial warranty period or for 90-days – whichever is greater.

5.0 WARRANTY