SOLICITATION_AG-6395-S-16-0017.pdf

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CODE 18a. PAYMENT WILL BE MADE BY

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OFFEROR

APHIS-MRPBS-ASD-6395

100 N 6TH STREET

Butler Square, 5th Floor ASD Procurement Branch

USDA APHIS MRPBS

CODE 16. ADMINISTERED BYCODE

X

X

X

115310

SIZE STANDARD:

100.00 % FOR:SET ASIDE:UNRESTRICTED ORAPHIS-MRPBS-ASD-6395

RFPIFB

10. THIS ACQUISITION ISCODE

RFQ

14. METHOD OF SOLICITATION

13b. RATING

NAICS:

SMALL BUSINESS

12/15/2015 1400 CS

11/13/2015

612-336-3364LISA DRAVES

(No collect calls)

INFORMATION CALL:

FOR SOLICITATION 8. OFFER DUE DATE/LOCAL TIMEb. TELEPHONE NUMBERa. NAME

4. ORDER NUMBER3. AWARD/ 6. SOLICITATION

AG-6395-S-16-0017

5. SOLICITATION NUMBER

SOLICITATION/CONTRACT/ORDER FOR COMMERCIAL ITEMS 1. REQUISITION NUMBER PAGE OF

1 75777910OFFEROR TO COMPLETE BLOCKS 12, 17, 23, 24, & 30

MINNEAPOLIS MN 55403

TELEPHONE NO.

17a. CONTRACTOR/

15. DELIVER TO

MINNEAPOLIS MN 55403

100 N 6TH STREET

Butler Square, 5th Floor ASD Procurement Branch

9. ISSUED BY

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2. CONTRACT NO.

EFFECTIVE DATE

$7.5

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TION UNLESS BLOCK IS

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13a.

SERVICE-DISABLED

VETERAN-OWNED

SMALL BUSINESS

HUBZONE SMALL

BUSINESS

8(A)

USDA APHIS MRPBS

WOMEN-OWNED SMALL BUSINESS

(WOSB) ELIGIBLE UNDER THE WOMEN-OWNED

SMALL BUSINESS PROGRAM

EDWOSB

24.

AMOUNT

23.

UNIT PRICE

22.

UNIT

21.

QUANTITY

20.

SCHEDULE OF SUPPLIES/SERVICES

19.

ITEM NO.

Emerald Ash Borer (EAB) Survey: Minnesota, Tennessee, Wisconsin, Michigan

This is a commercial solicitation for insect trap placement, maintenance, inspection, removal, and disposal; identification of suitable sites and trees within designated cells; visual surveys of the environment for ash trees exhibiting signs and symptoms of emerald ash borer (EAB) in proximity of the traps; sign placement; submission of suspect EAB specimen samples; data management;

and distribution of educational material where appropriate.

Please see the Pricing Schedule and Scope of Work beginning on page 5.

(Use Reverse and/or Attach Additional Sheets as Necessary)

HEREIN, IS ACCEPTED AS TO ITEMS:

XX

DATED . YOUR OFFER ON SOLICITATION (BLOCK 5),

INCLUDING ANY ADDITIONS OR CHANGES WHICH ARE SET FORTH

COPIES TO ISSUING OFFICE. CONTRACTOR AGREES TO FURNISH AND DELIVER

ARE

ARE

31c. DATE SIGNED

27b. CONTRACT/PURCHASE ORDER INCORPORATES BY REFERENCE FAR 52.212-4. FAR 52.212-5 IS ATTACHED. ADDENDA

31a. UNITED STATES OF AMERICA (SIGNATURE OF CONTRACTING OFFICER)

30c. DATE SIGNED 31b. NAME OF CONTRACTING OFFICER (Type or print)

ALL ITEMS SET FORTH OR OTHERWISE IDENTIFIED ABOVE AND ON ANY ADDITIONAL

SHEETS SUBJECT TO THE TERMS AND CONDITIONS SPECIFIED.

27a. SOLICITATION INCORPORATES BY REFERENCE FAR 52.212-1, 52.212-4. FAR 52.212-3 AND 52.212-5 ARE ATTACHED. ADDENDA

26. TOTAL AWARD AMOUNT (For Govt. Use Only)

OFFER

STANDARD FORM 1449 (REV. 2/2012)

Prescribed by GSA - FAR (48 CFR) 53.212

ARE NOT ATTACHED.

ARE NOT ATTACHED.

AUTHORIZED FOR LOCAL REPRODUCTION

PREVIOUS EDITION IS NOT USABLE

30b. NAME AND TITLE OF SIGNER (Type or print)

30a. SIGNATURE OF OFFEROR/CONTRACTOR

28. CONTRACTOR IS REQUIRED TO SIGN THIS DOCUMENT AND RETURN

25. ACCOUNTING AND APPROPRIATION DATA

29. AWARD OF CONTRACT:

REF.

32e. MAILING ADDRESS OF AUTHORIZED GOVERNMENT REPRESENTATIVE

32c. DATE 32b. SIGNATURE OF AUTHORIZED GOVERNMENT REPRESENTATIVE

ACCEPTED, AND CONFORMS TO THE CONTRACT, EXCEPT AS NOTED:

32a. QUANTITY IN COLUMN 21 HAS BEEN

RECEIVED INSPECTED

40. PAID BY39. S/R VOUCHER NUMBER38. S/R ACCOUNT NUMBER

37. CHECK NUMBER

FINALPARTIAL

36. PAYMENT

FINALPARTIAL

35. AMOUNT VERIFIED

CORRECT FOR

34. VOUCHER NUMBER33. SHIP NUMBER

COMPLETE

32g. E-MAIL OF AUTHORIZED GOVERNMENT REPRESENTATIVE

42d. TOTAL CONTAINERS42c. DATE REC'D (YY/MM/DD)

42b. RECEIVED AT (Location)

42a. RECEIVED BY (Print)

41c. DATE41b. SIGNATURE AND TITLE OF CERTIFYING OFFICER

41a. I CERTIFY THIS ACCOUNT IS CORRECT AND PROPER FOR PAYMENT

STANDARD FORM 1449 (REV. 2/2012) BACK

24.

AMOUNT

23.

UNIT PRICE

22.

UNIT

21.

QUANTITY

20.

SCHEDULE OF SUPPLIES/SERVICES

19.

ITEM NO.

32f. TELEPHONE NUMBER OF AUTHORIZED GOVERNMENT REPRESENTATIVE

32d. PRINTED NAME AND TITLE OF AUTHORIZED GOVERNMENT REPRESENTATIVE

752 of

AG‐6395‐S‐16‐0017

NOTES TO QUOTERS

a. QUOTATION REQUIREMENTS.

Quoters are advised to carefully review the quotation submission requirements specified in 52.212‐2 EVALUATION – COMMERCIAL ITEMS (Section 7.0, Solicitation Provisions) on page 52.

Quotations must include the following:

1. Completed SF 1449 (blocks 17a, 30a, 30b, 30c)

2. Completed Pricing Schedule

3. The mandatory submission requirements for each evaluation factor, as instructed in clause

52.212‐2 EVALUATION – COMMERCIAL ITEMS

4. Acknowledgement of Amendments (if applicable)

This is a best value trade‐off procurement. The Government reserves the right to award to other than the lowest quoter.

Vendors are responsible for ensuring completeness of quotations. The Government may evaluate solely on the information provided in the initial quotation. Quotations that fail to furnish the required information or reject the terms and conditions of the solicitation may be excluded from consideration.

b. SUBMISSION. Quotations shall be submitted by email to Lisa Draves at lisa.a.draves@aphis.usda.gov. Quotations must be received at or before the exact time specified in block 8 on the first page.

c. INQUIRIES. Questions concerning this solicitation should be directed to Lisa Draves by email at lisa.a.draves@aphis.usda.gov. (See provision 452.204‐70.)

d. CONTRACT TYPE. This solicitation will result in an Indefinite‐Delivery Indefinite‐Quantity contract.

Contract and task order minimums and maximums are addressed in the following clauses: See 52.216‐19, “Order Limitations” and 452.216‐73 “Minimum and Maximum Contract Amounts”. The line item quantities listed in the Pricing Schedule are estimates for evaluation purposes only and are not intended to convey either a minimum or maximum quantity that will be ordered by the Government. The unit price that will be paid for any line item will not be changed based on the quantity actually ordered by the Government. The ultimate contract price for any given ordering period will be the sum of the task orders issued during that period.

e. ISSUANCE OF TASK ORDERS. Work will be ordered through the issuance of individual task orders as the requirements of the U.S. Department of Agriculture (USDA) are defined. Each task order will include the specific requirements related to the work covered by the task order, and delivery or performance requirements. Task Order prices will be in accordance with the fixed rates established in the Pricing Schedule.

f. In accordance with Federal Acquisition Regulations (FAR) Subpart 9.1, a prospective contractor may be requested to provide additional information in order to assist the Government in making a responsibility determination.

g. Quoters must register and complete online representations and certifications in the System for Award Management (SAM) at www.sam.gov prior to the solicitation closing date. The

Government will review completed Representations and Certifications in SAM following the solicitation closing date. Submission of completed representations and certifications is NOT required with quotation submissions.

h. Evaluation of quotations will be based, among other factors, upon the total price included on the pricing schedule for all items. Quotations must include a price for all items in this solicitation to be eligible for award. The Government will award on an “All or None” basis.

i. USDA is an agency of the Federal Government and is tax exempt (Tax ID# 41‐0696271).

PRICING SCHEDULE

ITEM DESCRIPTION QTY UNIT UNIT PRICE AMOUNT

BASE Date of Award through December 31, 2016

Purple Prism Traps Only

0001 EAB Survey in Minnesota: Install Purple Prism Traps 1115 EA ___________ ___________

0002 EAB Survey in Minnesota: Service Purple Prism Traps 1115 EA ___________ ___________

0003 EAB Survey in Minnesota: Remove Purple Prism Traps 1115 EA ___________ ___________

0004 EAB Survey in Tennesse: Install Purple Prism Traps 1083 EA ___________ ___________

0005 EAB Survey in Tennesse: Service Purple Prism Traps 1083 EA ___________ ___________

0006 EAB Survey in Tennesse: Remove Purple Prism Traps 1083 EA ___________ ___________

0007 EAB Survey in Wisconsin: Install Purple Prism Traps 928 EA ___________ ___________

0008 EAB Survey in Wisconsin: Service Purple Prism Traps 928 EA ___________ ___________

0009 EAB Survey in Wisconsin: Remove Purple Prism Traps 928 EA ___________ ___________

0010 EAB Survey in Michigan: Install Purple Prism Traps 148 EA ___________ ___________

0011 EAB Survey in Michigan: Service Purple Prism Traps 148 EA ___________ ___________

0012 EAB Survey in Michigan: Remove Purple Prism Traps 148 EA ___________ ___________

TOTAL ‐ BASE PERIOD (0001 ‐ 0012) ___________

OPTION 1 January 1, 2017 through December 31, 2017

Purple Prism Traps

1001 EAB Survey in Minnesota: Install Purple Prism Traps 1115 EA ___________ ___________

1002 EAB Survey in Minnesota: Service Purple Prism Traps 1115 EA ___________ ___________

1003 EAB Survey in Minnesota: Remove Purple Prism Traps 1115 EA ___________ ___________

1004 EAB Survey in Tennesse: Install Purple Prism Traps 1083 EA ___________ ___________

1005 EAB Survey in Tennesse: Service Purple Prism Traps 1083 EA ___________ ___________

1006 EAB Survey in Tennesse: Remove Purple Prism Traps 1083 EA ___________ ___________

1007 EAB Survey in Wisconsin: Install Purple Prism Traps 928 EA ___________ ___________

1008 EAB Survey in Wisconsin: Service Purple Prism Traps 928 EA ___________ ___________

1009 EAB Survey in Wisconsin: Remove Purple Prism Traps 928 EA ___________ ___________

1010 EAB Survey in Michigan: Install Purple Prism Traps 148 EA ___________ ___________

1011 EAB Survey in Michigan: Service Purple Prism Traps 148 EA ___________ ___________

1012 EAB Survey in Michigan: Remove Purple Prism Traps 148 EA ___________ ___________

Green Funnel Traps

1013 EAB Survey in Minnesota: Install Green Funnel Traps 154 EA ___________ ___________

1014 EAB Survey in Minnesota: Service Green Funnel Traps 154 EA ___________ ___________

1015 EAB Survey in Minnesota: Remove Green Funnel Traps 154 EA ___________ ___________

1016 EAB Survey in Tennesse: Install Green Funnel Traps 171 EA ___________ ___________

1017 EAB Survey in Tennesse: Service Green Funnel Traps 171 EA ___________ ___________

1018 EAB Survey in Tennesse: Remove Green Funnel Traps 171 EA ___________ ___________

1019 EAB Survey in Wisconsin: Install Green Funnel Traps 128 EA ___________ ___________

1020 EAB Survey in Wisconsin: Service Green Funnel Traps 128 EA ___________ ___________

1021 EAB Survey in Wisconsin: Remove Green Funnel Traps 128 EA ___________ ___________

1022 EAB Survey in Michigan: Install Green Funnel Traps 19 EA ___________ ___________

1023 EAB Survey in Michigan: Service Green Funnel Traps 19 EA ___________ ___________

1024 EAB Survey in Michigan: Remove Green Funnel Traps 19 EA ___________ ___________

TOTAL ‐ OPTION 1 (0001 ‐ 1024) ___________

OPTION 2 January 1, 2018 through December 2018

Purple Prism Traps

2001 EAB Survey in Minnesota: Install Purple Prism Traps 1115 EA ___________ ___________

2002 EAB Survey in Minnesota: Service Purple Prism Traps 1115 EA ___________ ___________

2003 EAB Survey in Minnesota: Remove Purple Prism Traps 1115 EA ___________ ___________

2004 EAB Survey in Tennesse: Install Purple Prism Traps 1083 EA ___________ ___________

2005 EAB Survey in Tennesse: Service Purple Prism Traps 1083 EA ___________ ___________

2006 EAB Survey in Tennesse: Remove Purple Prism Traps 1083 EA ___________ ___________

2007 EAB Survey in Wisconsin: Install Purple Prism Traps 928 EA ___________ ___________

2008 EAB Survey in Wisconsin: Service Purple Prism Traps 928 EA ___________ ___________

2009 EAB Survey in Wisconsin: Remove Purple Prism Traps 928 EA ___________ ___________

2010 EAB Survey in Michigan: Install Purple Prism Traps 148 EA ___________ ___________

2011 EAB Survey in Michigan: Service Purple Prism Traps 148 EA ___________ ___________

2012 EAB Survey in Michigan: Remove Purple Prism Traps 148 EA ___________ ___________

Green Funnel Traps

2013 EAB Survey in Minnesota: Install Green Funnel Traps 154 EA ___________ ___________

2014 EAB Survey in Minnesota: Service Green Funnel Traps 154 EA ___________ ___________

2015 EAB Survey in Minnesota: Remove Green Funnel Traps 154 EA ___________ ___________

2016 EAB Survey in Tennesse: Install Green Funnel Traps 171 EA ___________ ___________

2017 EAB Survey in Tennesse: Service Green Funnel Traps 171 EA ___________ ___________

2018 EAB Survey in Tennesse: Remove Green Funnel Traps 171 EA ___________ ___________

2019 EAB Survey in Wisconsin: Install Green Funnel Traps 128 EA ___________ ___________

2020 EAB Survey in Wisconsin: Service Green Funnel Traps 128 EA ___________ ___________

2021 EAB Survey in Wisconsin: Remove Green Funnel Traps 128 EA ___________ ___________

2022 EAB Survey in Michigan: Install Green Funnel Traps 19 EA ___________ ___________

2023 EAB Survey in Michigan: Service Green Funnel Traps 19 EA ___________ ___________

2024 EAB Survey in Michigan: Remove Green Funnel Traps 19 EA ___________ ___________

TOTAL ‐ OPTION 2 (2001 ‐ 2024) ___________

TOTAL AMOUNT BASE AND OPTIONS (0001 ‐ 2024) ___________

1.0 SCOPE OF WORK

1.1 Background

The emerald ash borer (EAB) is a very small but very destructive wood‐boring beetle. The adult EAB is metallic green, cigar‐shaped, measures about 1/2‐inch long, and can fly. This devastating pest was first found in 2002 in North America around the Detroit area. On this continent, we know the EAB attacks ash trees (Fraxinus spp.), and all the species, including green, white, black, and blue are at risk. The EAB is responsible for the death and decline of tens of millions of ash trees.

Survey for EAB has undergone an evolution of tactics, scope, and application since its discovery. Survey was originally based on visually detectable symptoms (exit holes, bark cracks, epicormic branching, woodpecker feeding sites, etc.) to determine presence or absence of EAB. Visual survey was applied at various levels of intensity and with various techniques (e.g., ground surveys, ladders, climbing devices, bucket trucks). Overall results were poor and newly infested areas were often left undiscovered. It soon became apparent that destructive sampling of suspect trees was necessary.

Artificially stressed (girdled) trap trees offered an alternative to visual survey and were adopted program‐wide in 2005. This technique was an improvement for defining or delimiting the extent of an EAB infestation and was used to evaluate areas treated for EAB (eradication cuts). Trap trees, however, are expensive to establish and evaluate, and offer liability problems for workers and the public.

Uniformity of survey is also an issue because of difference in size, species, and locations of trees as well as methodology and timing of stressing prior to flight.

Continued developments in trap and lure design enabled the EAB Program to implement a survey based on attractant‐baited traps in 2008. Traps offer several advantages over trap trees including lower cost, uniformity of sampling unit, greater safety, fewer logistical problems, and more precision in sampling.

Based on the positive results and feedback from the trap‐based 2008 through 2015 surveys, APHIS’s EAB National survey will continue to rely primarily on purple prism traps. However, a limited number of Lindgren green multi‐funnel traps may also be incorporated into surveys. The green Lindgren funnel trap exhibits higher catch rates than the purple prism trap and can be reused from year to year but requires more frequent monitoring than the prism traps.

Since 2012, trap placement has been based on a survey sampling design developed in collaboration between the APHIS EAB Program and the U.S. Forest Service’s Forest Health Technology Enterprise Team (FHTET). This EAB survey will use the similar methodology. This computer‐generated EAB survey sampling design product combines a scientific model of the likelihood of detecting EAB with historical program data and regulatory knowledge. Very simply stated, the survey sampling design model will pre‐ select geographic locations (one square kilometer cells) to deploy EAB traps resulting in the highest probability of pest detection. The expected benefit of the EAB survey is three‐fold:

• To increase the number of instances of successful EAB detections outside the known infested area

• To improve land managers capability of detecting EAB close to the date of a new attack

• To find locations that are best suited to implement controls

For additional information see Appendix A: Details of Survey Sampling Design Construction.

1.2 Communication and media

The highly visible purple prism traps and green multi‐funnel traps generate curiosity which often results in public questions and media inquiries. Individuals involved in the actual placement and maintenance of traps should be conversant with survey messages to answer a limited number of questions (who, what, when, where, why). Refer anyone seeking more in depth information or any media inquiries to the APHIS EAB hotline 1‐866‐322‐4512.

1.3 Survey Objectives

The objectives of this survey are to determine whether additional pockets of infestation may exist undetected surrounding the known infested areas, to better define the leading edge, and to identify areas for biological control and mitigation activities. This survey will target sites at risk for the introduction and establishment of EAB through risk‐based modelling. In addition, the highly visible survey supports public outreach thereby increasing awareness and reporting of the pest. Contract task orders will specify a list of potential trap locations (cells) designated by latitude, longitude, and county.

A trap shall be set in each suitable one square kilometer cell. The criteria for trap placement within each designated cell are provided in the Trap Placement Protocols section of this document.

2.0 Conducting surveys and inspections

The period of performance shall be for one (1) base period and two (2) option periods. See 52.216‐18 ORDERING (OCT 1995) and 52.216‐22 INDEFINITE QUANTITY.

In general, the Contractor is responsible for the following activities

a) Identification of ash tree species (Fraxinus spp.)

b) Identification of most suitable site and ash tree within the designated cell

c) Placement and maintenance of traps, including downed and missing traps.

d) Inspection, suspect specimen collection and disposal of traps

e) Visual survey of the environment for ash trees exhibiting signs and symptoms of EAB in proximity of the traps.

f) Distribution of educational material where appropriate (supplied by APHIS)

g) Compilation of survey data and entry/upload into Government approved database

Research indicates that an accumulation of 450 growing degree days (base 50oF) results in initial emergence of EAB adults. Traps should be placed just prior to 450 growing degree days (GDD) and lure replacement is to occur just prior to the 60 day expiration date (see Appendix B for estimated date of initial emergence.) USDA preference is for the traps to be deployed prior to or as close as possible to 450

GDD.

For Purple Prism traps the EAB lures efficacy is approximately 60 days. Therefore traps shall be serviced 60 days after initial deployment. This check includes replacing the lure and the collection of any suspect insects.

Regardless of the initial placement date and the first check date, the traps shall not be removed prior to 1500 growing degree days or August 1st whichever is LATER.

Service checks for Green Funnel Traps shall occur every 14‐21 days after initial deployment and entails the collection of any suspect EAB and replenishment or replacement of polypropylene glycol in the collection cup. EAB lure efficacy is approximately 60 days, therefore at 60 days change the lure at that service check.

Regardless of the initial placement date and the first check date the traps shall not be removed prior to 1500 growing degree days or August 1st whichever is LATER.

If necessary the contractor shall rehang and/or relocate traps that are reported as fallen or placed in locations not desired by property owners.

Timing of trap placement is based on scientific data outlining the growing degree modeling for each State (see Appendix B)

Before commencing work, the contractor shall attend a USDA sponsored training session to learn specifics about EAB survey methodology.

3.0 Trap Placement

The APHIS PPQ sampling design generates a science‐based, objective survey. The sampling design identifies cell locations for the placement of traps with each cell identified by latitude and longitude and county and state.

Trap placement in each state shall be coordinated with the USDA State Plant Health Director (SPHD) who will provide overall coordination between the State Plant Regulatory Officials (SPRO), Tribal Governments (where Tribal lands are involved), other Federal, State, and local agencies, and non‐ governmental organizations (e.g., the Contractor) involved in the program. To ensure the highest probability of detecting EAB, traps shall be set in ash trees that meet the criteria as described in section

3.3.3 Trap Installation.

The list of trap cell locations generated by the survey sampling design includes the latitude and longitude for the center point of each cell in which a trap is to be placed. Navigating to each cell will require the use of a global positioning system (GPS) unit. Set‐up and guidance information for GPS units is provided in Appendix C.

GPS coordinates will be provided in decimal format to at least four digits past the decimal; for example,

42.1234 and ‐76.1234.

The Contractor is responsible for assessing viability of cells for trap placement. The Contractor is responsible for downed and missing traps. Additional compensation will not be provided for these items.

3.1.1 Cell Designations

For various reasons it may not be possible to place a trap in a cell as designated by the survey design.

These cells must be recorded in the USDA‐approved database according to the following Omit Reason options:

Omitted Reason:

Dangerous neighborhood (i.e. high crime area, vicious dogs, etc.)

Landowner refusal

No ash present in cell

No access, other difficult terrain

No access, steep slope

No access, swamp

No road access

Other

3.1.2 Alternate Cell Sites

The survey sampling design will generate alternate cell sites for each State equivalent to 20% of their allotted traps. This list shall be used to place an omitted trap at an alternate site.

Coordinate with the Contracting Officer’s Representative (COR) on placement of traps in Alternate Cell Sites.

3.1.3 Destructive Sampling

While the Contractor is not responsible for destructive sampling, if they discover a tree exhibiting two or more of the symptoms listed below they shall refer that tree (GPS location) to the Field Representive of the respective state:

• Canopy stress/dieback

• Epicormic shoots/suckering

• Bark splits

• Woodpecker damage

• D‐shaped exit holes (3‐4 mm diameter)

• Serpentine larval galleries

3.2 Equipment

3.2.1 Items Supplied by the Government

If an item is not listed below it will not be provided by the Government. (See also paragraphs 3.7.1 and 3.7.2.)

Purple Prism Traps (unassembled) Green Funnel Traps (unassembled) Lures Spreaders Tree Limb Hooks Trap Hanging Devices – (Modified Paint Rollers) “Do not disturb” sign

Delivery

Supplies will be delivered to no more than three (3) locations per state on average. The Government will work with selected vendor to ensure that optimal timing of lures and traps is achieved. No more than three (3) shipments per location will be scheduled.

3.2.2 Contractor Supplied Items

While this list is not all inclusive (and not all items may be necessary) below are some of the items the Contractor is responsible to provide in order to conduct the survey.

Individual identification Cable ties (6” long for lure attachment) Propylene glycol (without ethanol) Ethanol Paper cone paint filters Telescoping pole (see 3.5 below) Throw rope Throw bag Cable ties (4 feet long ‐ 2/trap to be fastened to tree) Parchment or wax paper Utility Knife Forceps, Bioquip #4750 ‐ rounded end featherweight or similar Small plastic zipper bag

Snack size bag‐‐available from any grocery store (6.5 in x 3.25 in) GSA plastic bag #8105‐00‐837‐7753 (4 in x 4 in) Data label ‐‐ should be printed on a 2"x 4" shipping label Specimen vials Permanent magic marker or pencil Hand Cleaner – Mechanic’s hand cream cleaner, baby oil, mineral oil (paper towels will also be necessary) Examination gloves (vinyl, nitrile, or latex) Small ground tarp (to avoid trap soiling during examination)

3.3 Prism Traps

3.4.1 Timing of Trap Placement

Research indicates that an accumulation of 450 growing degree days (base 50oF) results in initial emergence of EAB adults. Traps should be placed just prior to 450 growing degree days (GDD) and lure replacement is recommended to occur just prior to the 60 day expiration date. In order to assist with timing of trap placement, a map of the conterminous U.S. depicting predictive bands of initial emergence are appended to this document (Please see Appendix D for GDD map). Appendix B defines, by state, when initial emergence is predicted to occur. In addition, weekly during the survey season the EAB program will provide a GDD map to assist with the timing of trap placement. This map will help in timing the placement of traps just prior to the emergence of EAB adults which occurs upon the accumulation of approximately 450 GDD.

3.3.1 Assembly

A prism trap consisting of three 14” x 24” panels with several holes for trap and lure attachment (Fig. 1) will be used. The trap is constructed from a sheet of pre‐glued purple corrugated plastic. An instructional video detailing trap assembly and use can be found at:

http://www.aphis.usda.gov/plant_health/plant_pest_info/emerald_ash_b/index.shtml clink on Pest and Disease Programs, then Emerald Ash Borer, and scroll down to Video ‐ Purple Trap

Instructional Video (windows media video).

After the trap is assembled into its prism shape (glue side facing out), spreaders are attached to the trap at holes labeled (3) in Fig. 1. T h e Lure is attached to a loop on the spreader using a cable tie (Fig. 2).

FIGURE 1: Prism trap diagram

FIGURE 2: Lure hung from loop on spreader using a cable tie

3.3.2 Lures

One pouch of lure containing Z‐3 Hexanol will be attached to each trap. The lure provided by commercial firms are produced as a pouch and designed to last in the field for 60 days. Lure attachments to the trap should be reinforced with duct tape placed at the very top to ensure continued attachment during extreme weather. Do not cover the lure with duct tape as that will inhibit the release of the lure. Any unused lure remaining at the end of the trapping season shall be stored (frozen) for future use in EAB surveys.

Lure Handling, Transport, and Storage:

The (Z)‐3‐hexanol lure pouches emit odors and volatiles which pose safety and health concerns in confined spaces. EAB Program Managers have worked closely with the APHIS Safety and Health Manager and the lure manufacturer to develop protocols for the safe handling, transport, and storage of our lures. In summary, prior to deployment, lures must be kept cold at all times by storing in a freezer. During transport, lures must be kept in coolers with cold packs or ice and stored in isolation from the vehicle’s cab, or, as an alternative, kept in coolers with screw top lids that also must be chilled with cold packs or ice. Cooler should be labeled “NOT FOR FOOD OR BEVERAGE USE”. To avoid skin irritation during handling, gloves may be worn. Additional information and precautions can be found in a Job Hazard Assessment and Material Safety Data Sheets for the lure pouches and are available from the local APHIS PPQ Safety Coordinator.

3.3.3 Trap Installation

Traps shall be placed in ash trees (Fraxinus spp.). If possible, ash trees should be 8” or greater in diameter with larger or largest ash tree in a stand of trees located along edges, in open areas, or in open stands such as in parks. Traps should be placed in the lower to mid canopy, but with the bottom edge of the trap no lower than five feet above the ground. They should be placed on the sunny side of the tree, most typically, the south or southwest side.

FIGURE 3: Trap hung using a telescoping pole

A wire hanger will be used to hang traps (Fig. 3). An 8’ telescoping “painters” pole that extends to 23’+ and can be fitted with the threaded paint roller hook to place the trap in one of the lower limbs is recommended.

The full extension of the pole may be necessary. Alternately, if all limbs are too high to reach with a pole, a throw line may be tossed over a limb, and a rope with a trap attached may be hoisted up into the canopy (Fig. 4).

FIGURE 4: Trap hung using a rope

3.3.4 Trap Maintenance

The EAB lure efficacy is approximately 60 days. Therefore traps shall be serviced as close as possible to 60 days after initial deployment. This check includes changing the lure and collecting any suspect insects

3.3.5 Servicing traps

1) Check traps for EAB (see 3.6 Sample Submission)

a) Traps will be checked for target specimens (first level screening) and any suspects should be placed in a vial per 3.6 Sample Submission

2) Replace/repair trap parts as needed such as wire supports, plastic spacers, and fasteners.

3) If trap surfaces are loaded with debris, they should be replaced with new traps.

3.5 Green Funnel Traps

3.5.1 Lures

A (Z)‐3‐hexanol lure pouch should be hung from one of the holes used for peg attachment near the top of the multi‐funnel trap with a small plastic cable tie. The lure should be changed after 60 days.

3.5.2 Timing of Trap Placement

The timing for placement of Lindgren green multi‐funnel traps is identical to the purple prism trap.

3.5.3 Trap

The trap is constructed from 12 green plastic funnels with a green dome‐shaped top and a plastic collection cup at the bottom. Trap funnels and the dome are held together by black pegs. The collection cup is secured to the bottom with a twist‐on motion. An eye screw at the top of the trap is used for rope attachment (see below). Multi‐funnel traps are pre‐assembled and pre‐coated in a 1:1 solution of Fluon before shipment to field locations. The Fluon application provides a slippery surface that assists with the capture of EAB. Due to the modular nature of the traps, some minor assembly may be required.

FIGURE 5: Multi‐funnel traps hanging in ash trees

3.5.4 Trap hanging

A rope should be thrown over a branch at approximately 16‐26’ above the ground. The branch should be alive and sturdy (> 2” diameter), as smaller or dead branches could snap under the weight of the trap.

An arborist throw weight (12, 14, or 16 oz.) can be tied to one end of the rope. Take care to clear the rope from all lower branches so that the trap can be pulled up and down like an elevator when servicing.

The rope should have a clear path to the ground. The trap may need to be raised and lowered at an angle from the tree to accomplish this. Give yourself enough slack so that you can tie the rope to an adjacent tree.

A knot, similar to the one shown here is the preferred method:

(http://www.fieldandstream.com/photos/gallery/fishing/bass/where‐fish/2009/02/strongest‐fishing‐ knots)

Using these knots, tie the rope to two points on each trap. For the multi‐funnel trap these points are the top metal hanger and one of the three holes on the bottom funnel used for peg attachment. The knot is important as high winds will sometimes cause the traps to come down if the knots are not tied properly.

When hanging the traps try to rest the top of the trap against the bottom of the tree limb. This should prevent the rope from twisting on itself. When attaching the collection cup, take care to make sure that it is securely attached to the bottom funnel.

FIGURE 6. Green multi‐funnel trap with attachment points for ropes and lures.

Prior to raising the trap, in the tree, fill the cup with approximately 2 inches of propylene glycol (RV antifreeze; usually pink in color). Do NOT use any product containing ethanol as products containing this will evaporate over time, especially in hot, dry climates or under drought conditions. Propylene glycol should not be diluted with water prior to filling the cup as it will be diluted by rainwater over time.

Once the trap is hoisted, tie it off to a tree (the one it is hanging from will work for this). When tying this rope off, the contractor shall ensure the rope is placed out of the reach of children or others who may want to pull it down.

3.5.5 Trap checking

When checking the multi‐funnel trap, the contents of the trap should be poured through a paper filter.

A paper cone paint strainer is the preferred method, although a coffee filter may also work but will retain more moisture. The paper filter should then be placed inside a leak proof plastic bag. While wire closed bags are the preferred method, a plastic zipper bag will also work. The bag shall be labeled and sealed completely to prevent leaks. A small amount (one teaspoon) of ethanol may be added to the bag to help preserve the specimens for sorting in the lab. As long as the liquid in the cup is still pink, and does not smell too badly, it can be re‐used instead of discarded during traps checks. Place a container for collection of the propylene glycol under the filter when pouring out the sample.

FIGURE 7. Paper cone paint strainer containing a sample of beetles

3.5.6 Trap Installation

Traps shall be placed in ash trees (Fraxinus spp.). If possible, ash trees should be 8” or greater in diameter with larger or largest ash tree in a stand of trees located along edges, in open areas, or in open stands such as in parks within the grid cell. Traps should be placed in the lower to mid canopy, but with the bottom edge of the trap no lower than five feet above the ground. They should be placed on the sunny side of the tree, most typically, the south or southwest side.

3.5.7 Trap Maintenance

The lure will last approximately 60 days and should be replaced just prior to expiration. Multi‐funnel traps shall be checked every two‐three weeks (and any insects that are captured should be collected).

When reattaching cups, the contractor shall make sure that all pegs are securely locked in place as they may come loose under some weather conditions.

3.5.8 Servicing traps

1) Check traps every two‐three weeks for EAB (see 3.6 Sample Submission)

a) Traps will be checked for target specimens (first level screening) and any suspects shall be placed in a vial with 70% ethanol, labeled with date and trap number, and submitted per 3.6 Sample Submission

2) Cleaning of collection cup and renewal of propylene glycol (; ethanol free);

3) Replace/repair trap parts as needed (wire supports, plastic spacers, fasteners, etc.)

3.5 Sign Placement

A 3”x5” survey sign (Fig. 8) will be provided and should be prominently displayed on the tree after the trap is set.

FIGURE 8: Survey sign

DO NOT DISTURB

Emerald Ash Borer Survey Tree United States Department of Agriculture

For EAB Survey information call 1‐866‐322‐4512

Learn more about invasive pests at www.HungryPests.com

3.6 Sample Submission

Adults:

Any suspect adult EAB specimen collected from a trap should be placed in a dry vial. Using the non‐drying sticky compound found on the trap, stick the specimen to the side of the vial and/or gently pad with soft tissue placed into the vial on top of the specimen(s) to prevent damage during shipping.

Sample Forms and Delivery All suspect specimens shall be delivered to Dr. James Zablotny at the address below, along with a completed “Specimens for Determination” PPQ form 391. The contractor shall include survey record number and/or GPS coordinates on the PPQ form 391 so identified specimens can be linked to survey records.

Dr. James Zablotny

USDA, APHIS, PPQ

11200 Metro Airport Center Drive, Suite 140 Romulus, MI 48174 Phone: 734‐229‐1672 E‐mail: james.e.zablotny@aphis.usda.gov

The Government will make a determination and send specimens to the Systematic Entomology Laboratory (SEL), if necessary, for initial State detection confirmation.

The contractor shall notify the respective COR of any suspect specimen submissions by COB on the day of submitting the sample.

3.7 Trap Removal

Regardless of the initial placement date and the first check date the traps shall not be removed prior to 1500 growing degree days or August 1st whichever is LATER. When date or temperature thresholds for removal occur, the contractor shall collect suspect specimens and remove all traps and Do Not Disturb signs.

3.7.1 Purple Prism Trap Disposal

The contractor, at their own expense, shall dispose of all traps, including removal of the Do Not Disturb sign, at the end of the survey. While the polypropylene material of the traps is recyclable, the glue that is adhered to the panels renders the traps undesirable to most recyclers. In the interest of conservation, Contractor shall make a reasonable effort to recycle or dispose of material offsite for some useful purpose.

Hangers and spreaders are reusable and shall be returned to the respective SPHD office at the contractor’s expense unless the contractor receives notification that the Government intends to exercise an option year in accordance with clause 52.217‐8 OPTION TO EXTEND SERVICES (NOV 1999). Hangers and spreaders that cannot be reused shall be recycled.

3.7.2 Green Funnel Trap Disposal

The contractor shall remove all traps and Do Not Disturb sign at the end of the survey. Multi‐funnel traps are designed to be used over several field seasons. At the end of the season, the collection cups shall be gently scrubbed, and dirt shall be wiped away from funnels with a cloth. The contractor is advised not to remove too much of the slippery Fluon coating. Traps will collapse into a smaller form for storage and subsequent reuse. Green funnel traps shall be returned to the SPHD at the contractor’s expense unless the contractor receives notification that the Government intends to exercise an option year in accordance with clause 52.217‐8 OPTION TO EXTEND SERVICES (NOV 1999).

3.8 Data Management Structure for Emerald Ash Borer (EAB)

The USDA‐approved database hereinafter referred to as the Integrated Plant Health Information System (IPHIS), will be the repository for all data. Data shall be compiled by the Contractor and shall be entered into the IPHIS database via one of the four data entry methods listed below. Access to IPHIS does not require Virtual Private Network access therefore anyone with internet access and an eAuthentication login will have access to IPHIS.

People without an eAuthentication username and password will need to register at: http://www.eauth.egov.usda.gov/

After receiving network access, a username, and password for IPHIS, users can log into the system and utilize any (or all) of the four (4) data entry methods. These tools include; IPHIS web interface, Field Adaptable Survey Tool (FAST) desktop application, a FAST PDA (Personal Data Assistant) software application, and an Excel spreadsheet upload. The web upload tool, an Excel spreadsheet, to be used for submission to IPHIS, is available at:

http://www.aphis.usda.gov/plant_health/plant_pest_info/emerald_ash_b/index.shtml click on Pest and Disease Programs, then Emerald Ash Borer, and scroll down to “Survey Data Spreadsheet”. A paper form for recording data for later input into the USDA‐approved database is also available and appears as Appendix E (EAB Trap Sheet).

We will no longer ask surveyors to collect symptom data. There are several data fields in IPHIS which were added starting with the 2012 EAB survey. These include: (1) a field for cells that are omitted, (2) a field to identify and record the relocated traps due to omits, and (3) an expanded field for comments.

We encourage users to use the mobile portion of IPHIS, but understand contractors may have existing tools and/or applications used to collect data in the field. Contractors utilizing methods other than mobile electronics (paper, spread sheets, or third party software platforms) can enter data directly into the web interface or “bulk” upload data from flat file spread sheets using the web upload tool.

The Contractor shall upload data within two weeks after traps are set, following servicing and rebaiting, and again when all traps are removed.

The actual final trap placement data will also be supplied to the respective SPHD.

Technical Support 1‐877‐944‐8457 (Available: 8am – 8pm EST) eAppshelp@3ht.com

Dave Kowalski IPHIS Data Manager

(970) 494‐7510 David.G.Kowalski@aphis.usda.gov

4.0 CONTRACT ADMINISTRATION

4.1 Invoicing Information

Invoices shall be submitted through the Invoice Processing Platform (www.ipp.gov)

Invoices may be processed upon completion of each of the 3 phases: trap placement, the first trap check, the second trap check and removal of the traps (e.g., 1/3 of the total per trap price) and the recording of data into IPHIS following each of the three phases. Copies of the invoices must be emailed to the COR and Program Office.

4.2 Contracting Officer’s Representative

The Contracting Officer will designate a Contracting Officer’s Representative (COR) at the time of award. The COR will be the Contractor’s local Government contact for contracts awarded against this requirement.

The COR is responsible for monitoring the performance of work under contracts awarded as written in the contract. In no event, however, will any understanding, agreement, modification, change order, or other matter deviating from the terms of the contract be effective or binding upon the Government unless advance authorization is received from the Contracting Officer, and proper contractual documents executed by the Contracting Officer prior to performing the work.

The COR shall inform the Contracting Officer as soon as possible of any recommended modifications, and actions or inactions of the Contractor or the Government which could impact the Contractor’s ability to comply with the contract performance requirements or completion times.

Whenever, in the opinion of the Contractor, the COR requests effort outside the scope of the contract, the Contractor shall so advise the COR. If the COR persists and there still exists a disagreement as to proper contractual coverage, the Contracting Officer shall be notified immediately.

PROCEEDING WITH WORK WITHOUT PROPER CONTRACTUAL COVERAGE AND APPROVAL OF THE

CONTRACTING OFFICER COULD RESULT IN A BREACH OF CONTRACT AND NONPAYMENT.

The designation (delegation of authority) shall be provided in writing to the COR and the Contractor.

The delegation of authority shall clearly explain the COR’s role and responsibility on the contract, and limitations of authority.

The following contacts are the only persons authorized to interact with the contractor on this contract:

1. For contract related issues:

Lisa Draves, Contract Specialist, (612) 336‐3364 Jim Roloff, Contracting Officer, (612) 336‐3223

Contracting Officer’s Representative (COR): TBD

5.0 APPENDICES

APPENDIX A. Details of Survey Sampling Design Construction.

The 2016 EAB survey employs a computer‐generated risk assessment product to guide the deployment of detection tools in a way which results in the following benefits:

increase the number of instances of successful EAB detections outside the known infested area improve land managers capability of detecting EAB close to the date of a new attack find locations that are best suited to implement biological control

The 2016 EAB Risk Assessment product was developed using an ensemble (or combination) of statistical models for predicting where EAB was most likely to be detected. This is a major change in modeling procedure over previous years which employed the MaxEnt statistical model, which used only positive detections for EAB. Given the last few years of nationally consistent data collection which reports both positive and negative detections, we upgraded the models to utilize that information in order to avoid some major statistical assumptions regarding the data. In doing so, we discovered that all the models concurred in predicting risk in the known infested area but differed widely in extrapolating that risk to outside the infested area. This is consistent with our knowledge concerning the difficulty of pest detection, so an “ensemble” of the models was created to mitigate model uncertainty between statistical platforms. There were two model applications for each statistical platform: One using all 2011‐2015 trap data (modeling full geographic potential) and one model using 2015 trap data only (active spread).

EAB is an actively spreading pest and a false negative could be reported for two reasons: 1) the location is suitable but not yet infested, or 2) the pest is present but undetected. Utilizing negative trap location could be too restrictive in the model fitting process because of the potential for false negatives. While MaxEnt demonstrates flexibility regarding the “potential” of locations of having a pest present, we found that MaxEnt’s default assumption of pest prevalence (EAB can occupy up to 50% of the landscape) caused great overestimation of risk compared to models that utilized actual absence information. Since the EAB risk model is updated each year, we are mitigating the issue by allowing the model to update with new information as the pest invades new environmental gradients.

Four parameters were required to run the models. These were:

1) the dependent detection data from the APHIS IPHIS dataset (2011‐2015), which consists of both negative and positive trap data

2) selected independent variables that correlate with the presence of EAB

3) a defined geographic area of interest.

4) limited to a maximum of 16,000 traps.

The independent variables were selected in the previous year using the following sources:

1. a review of scientific literature

2. expert opinion

3. anecdotal evidence

4. correlation analyses

5. preliminary statistical analyses.

Dissemination of trap resources is thus determined by this EAB Risk Assessment, combined with a spatially balanced sample design. The EAB Risk Assessment model has a known inclusion probability for every grid cell and provides an objective and transparent methodology for selecting trap locations. Since we are utilizing an ensemble model which is actually a percentage of model agreement of pest presence (rather than risk of pest presence like in previous years), the sample design will utilize this ranking of model agreement as the inclusion probability surface. The 2016 EAB sampling design increases spatial independence among sample locations and therefore, maximizes sampling efficiency and provides the most information available per sample unit.

Each trap location is recommended for placement within a one kilometer square grid cell designated within a Conterminous United States (CONUS) grid cell, each with a unique ID, and also by the center point of the grid cell (identified by latitude and longitude).

APPENDIX B. Predicted Growing Degree Day Zones for Initial EAB Emergence.

Alabama 2/1/2016

Alaska 6/22/2016

Arizona 2/1/2016

Arkansas 3/15/2016

California 2/15/2016

Colorado 4/22/2016

Connecticut 5/22/2016

Delaware 4/22/2016

Florida 1/22/2016

Georgia 2/15/2016

Hawaii 1/22/2016

Idaho 5/22/2016

Illinois 4/22/2016

Indiana 4/22/2016

Iowa 5/1/2016

Kansas 4/8/2016

Kentucky 4/8/2016

Louisiana 2/15/2016

Maine 6/1/2016

Maryland 4/22/2016

Massachusetts 5/22/2016

Michigan 5/22/2016

Minnesota 5/22/2016

Mississippi 2/22/2016

Missouri 4/1/2016

Montana 6/1/2016

Nebraska 5/1/2016

Nevada 2/22/2016

New Hampshire 6/1/2016

New Jersey 5/8/2016

New Mexico 3/15/2016

New York 5/15/2016

North Carolina 3/22/2016

North Dakota 6/1/2016

Ohio 5/1/2016

Oklahoma 3/5/2016

Oregon 5/22/2016

Pennsylvania 5/1/2016

Rhode Island 6/1/2016

South Carolina 3/8/2016

South Dakota 5/15/2016

Tennessee 4/1/2016

Texas 1/22/2016

Utah 5/11/2016

Vermont 6/1/2016

Virginia 4/8/2016

Washington 5/22/2016

West Virginia 5/1/2016

Wisconsin 5/15/2016

Wyoming 5/22/2016

APPENDIX C. GPS Guidance and Sample Location Information

Initial Requested Information

1. Field Crew Name

a. Crew ID (State and Affiliation (i.e., PPQ, State Dept. of Ag., Contractor, Volunteer) should be associated to the Crew ID)

Initial GPS Unit Setup Guidance

1. Depending on your GPS unit and what options that are available to you, please use the following guidance for collecting position information.

a. WGS 84 geodetic datum

b. Latitude/Longitude collected in Decimal Degrees format

c. Satellite Almanac (where applicable): Download the most recent Satellite Almanac to your unit.

d. Wide Area Augmentation System (WAAS) to improve the accuracy of the positions

Helpful Tips for Collecting Information at the Site

1. Turn the GPS unit on and allow the GPS unit to start gathering data when you arrive at the tree in which the trap will be hung. It may be helpful to turn the GPS unit on when you leave your vehicle.

2. Place the unit in a stable location while hanging the trap.

3. Collect location data at the tree in which the trap is hung

4. Ensure that you are collecting a signal from at least four satellites.

5. Location Data Collection

a. Location Name for Grid Cell = Cell_ID

i. When recording the Cell_ID (Location Name) in IPHIS, the Samp_ST_ID code assigned to each cell designated as a trap placement site by the survey design should be used if available. For cells which do not have a Samp_ST_ID code assigned, please use the following naming convention: 2 letters for State designation, 3 letters for surveyor’s initials, and 3 numbers for trap number, e.g., IAJAD003, (Iowa, John A. Doe, Trap 3). Each surveyor should begin with

001 for the first trap which does not have an assigned Cell_ID and increased by one for each subsequent trap (the second such trap is 002; the third is 003 and so on).

b. Please see Appendix D for EAB Field Data form.

c. Latitude/Longitude

d. Omitted ‐ with drop down list of potential reasons for the…