D-Attachment-Questions.pdf

PDF 415 KB Posted

Attached to
Long read sequencing services Federal contract opportunity
Solicitation number
75N95025Q00023
Issued by
Department of Health and Human Services National Institutes of Health National Institute on Drug Abuse

About this file

This is a Questions and Answers document for RFQ #75N95025Q00023 for long read sequencing services, containing four contractor questions and government responses.

The Q&A clarifies that for optional DNA extraction, acceptable sample sources include human whole blood in EDTA, plasma, cultured cells, or brain tissue. Three requirements were removed from the Statement of Work (SOW): expression profiling for RNA analysis, de-novo assembly, and custom analysis solutions. These were replaced with a single requirement that "Contractor shall provide structural variant and variant calling on a per sample basis."

View the file

Other files for this federal contract opportunity

On GovTribe

Work with this file on GovTribe

  • Download the original file
  • Contacts named in this file
  • Similar government files
  • Ask GovTribe AI about this file

Text version

Question No. Question Answer

For the optional DNA extraction, what sample source will be submitted? For example, is it human whole blood in EDTA, plasma, etc.?

Any of these starting material samples could potentially be provided: human whole blood in EDTA, plasma, or cultured cells, or brain tissue

Expression profiling is only available for RNA analysis, not DNA. Thus, cannot be provided. Was this requested in error?

Yes. This was removed from the SOW and the new specific bullet point now reads “Contractor shall provide structural variant and variant calling on a per sample basis.

3 Is de-novo assembly really required?

De-novo assembly not required. This was removed from the SOW and the new specific bullet point now reads “Contractor shall provide structural variant and variant calling on a per sample basis.

Can we please get a definition of the “custom analysis solutions” so we know how to price the specification?

This was removed from the SOW and the new specific bullet point now reads “Contractor shall provide structural variant and variant calling on a per sample basis.

QUESTIONS RECEIVED FOR RFQ# 75N95025Q00023 Long read sequencing services

File details come from the government source that posted it. Updated .