SON 7249860 v2_Redacted.pdf
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- Attached to
- 10X Genomics Reagent Kits for NIDDK Federal contract opportunity
- Solicitation number
- 75N94025Q00181
About this file
This Statement of Need details a purchase order for 10x Genomics Reagent Kits by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK). The procurement objective is to acquire a bundled set of consumables and an instrument upgrade for single-cell and spatial transcriptomics projects, including Single-cell RNA-seq reagents, Single-cell RNA + TCR-seq kits for immune-repertoire profiling, Spatial transcriptomics reagents for Xenium In Situ, Visium HD, and Visium HD-3' workflows, and an upgrade of their existing Chromium Controller to a CytAssist.
The document specifies detailed requirements for multiple 10x Genomics products, such as Chromium GEM X Single Cell 3 and 5 kits, Xenium Prime mouse tissue kits, Visium HD mouse packs, GEM-X Flex kits, and associated reagents. The purchase order is fixed-price, non-severable, with delivery required within 60 days after receipt of order to the NIH NIDDK facility in Bethesda, Maryland. The Project Officer will be responsible for monitoring technical progress, interpreting work requirements, performing technical evaluations, and assisting in resolving any performance issues.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Combined Synopisis-Solicitation 75N94025Q00181.pdf | ||
| JA 7249860 final_Redacted.pdf | ||
| Attachment 3 - NIH Invoice and Payment Provisions.pdf | ||
| Attachment 1 - Statement of Need_Redacted.pdf | ||
| Attachment 2 - Evaluation Criteria.pdf | ||
| Attachment 4 - FAR 52.212-5 (JAN 2025) [(DEVIATION FEB 2025)] 75N94025Q00181.pdf |
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STATEMENT OF NEED
1.0 PURCHASE ORDER TITLE
10X Genomics Reagent Kits for NIDDK
2.0 BACKGROUND
The NIDDK explores how cells maintain homeostasis in various tissues of the digestive, kidney, and related systems and how that balance breaks down to drive disease. To pinpoint the molecular switches that tip systems of the body from healthy to harmful, our institute profiles tissue resident and homing cells (such as inflammatory immune cells) directly within affected tissue.
Standard bulk and microdissection spatial methods average signals across millions of cells and mask the critical differences that spark disease. The 10x Genomics single-cell and spatial kits solve this problem.
Single-cell RNA-seq tells NIDDK which genes are turned on, while spatial workflows let us pinpoint where those cells and molecules are pinpointed within tissue. Merging these readouts gives us a high-resolution atlas of gene expression across tissues and disease states with healthy controls. These data are essential for the NIDDK mission: they reveal the exact pathways that malfunction, identify new drug targets, and guide the development of precision therapies to halt or diseases of major body system organs.
3.0 OBJECTIVE
The objective of this procurement is to acquire a bundled set of 10x Genomics consumables and an instrument upgrade needed for single-cell and spatial transcriptomics projects, as follows: Single-cell RNA-seq reagents (Chromium Next GEM v3.1 chemistry), Single-cell RNA + TCR-seq kits for comprehensive immune-repertoire profiling, Spatial transcriptomics reagents for the Xenium In Situ, Visium HD, and Visium HD-3′ workflows, and an instrument trade-up/upgrade of our existing Chromium Controller to a CytAssist. Together, these items will allow high-resolution mapping of cellular states and spatial gene expression across multiple NIDDK projects.
4.0 REQUIREMENTS
We require the following 10x Genomics brand-name items:
Part No. Manufacturer Description UOM Qty
GEM-X 3'. 4-rxn
1000690 10X Genomics Chromium GEM X Single Cell 3 KIT 1
1000686 10X Genomics Chromium GEM X Single Cell 3 KIT 1
Xenium Prime mouse with add-on. 4-rxn.
1000672 10X Genomics Xenium Prime 5K Mouse Pan Tissue and Pathways Assa Each 2
1000766 10X Genomics Xenium Prime 5K Add on Each 1
Visium HD mouse. 3' and Flex
1000674 10X Genomics Visium HD Mouse Pack 1
1000691 10X Genomics Chromium GEM X Single Cell 3 KIT 2
1000690 10X Genomics Chromium GEM X Single Cell 3 KIT 2
1000699 10X Genomics Chromium GEM X Single Cell 5 KIT 2
1000698 10X Genomics Chromium GEM X Single Cell 5 KIT 2
1000781 10X Genomics GEM-X Flex Sample Preparation v2 Kit, 48 rxns Each 1
1000797 10X Genomics GEM-X Flex Gene Expression Mouse 4-plex, 16 sample Each 2
1000791 10X Genomics GEM-X Flex Gene Expression Chip Kit, 4 chips Each 1
GEM-X 5' TCR. 8-rxn
1000254 10X Genomics Chromium Single Cell Mouse Each 2
1000694 10X Genomics Library Construction Kit C KIT 2
1000695 10X Genomics Chromium GEM X Single Cell 5 KIT 2
1000698 10X Genomics Chromium GEM X Single Cell 5 KIT 2
Controller trade-up of Chromium Controller instrument serial # 30593
1000441 10X Genomics Visium CytAssist Each 1
1000499 10X Genomics Visium CytAssist Reagent Each 1
1000886 10X Genomics Visium CytAssist Alignment Aid Each 1
1000857 10X Genomics Visium HD 3', 6.5 mm, 4 rxns Each 1
1000854 10X Genomics Visium HD 3’ Reagents - Kit A Small Each 1
N/A N/A Delivery/Shipping Total 1
Delivery Location:
National Institutes of Health, NIDDK Bldg 10 / /Room 10S227 9000 Rockville Pike Maryland 20892
5.0 PURCHASE ORDER TYPE
Fixed-price, Delivery Order, Non-Severable
6.0 PERIOD OF PERFORMANCE
Delivery by 60 Days After Receipt of Order
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