47PE0721Q00080003 King Building IAQ and Particulate Analysis.pdf

LEAD AND COPPER IN WATER TESTING

Survey location:

General Services Administration James Lawrence King Federal Building 99 NE 4th Street Miami, Florida 33132

GSA Building Number: FL1021 Project Reference Numbers: 545791, 545792, 590182

Survey date: May 13, 2020 Report date: June 2, 2020

Prepared By:

Environmental Health and Safety Services Program Support Center U.S. Department of Health and Human Services

INDOOR AIR QUALITY SURVEY

COMPLETED FOR THE

GENERAL SERVICES ADMINISTRATION

1.0 EXECUTIVE SUMMARY

On May 13, 2020, Mr. Matthew Berry, representing the U.S. Public Health Service, Federal Occupational Health, conducted an indoor air quality monitoring which included collecting fungal air samples, and recording levels of carbon dioxide, carbon monoxide, temperature and relative humidity on floors 2, 3, 4, 6, 7 and 8 in the James Lawrence King Federal Building located at 99 NE 4th Street in Miami, Florida (GSA Building Number: FL1021). This biannual monitoring was requested in response to employee concerns related to suspected mold growth in the space. The air monitoring will be conducted every six months.

The James Lawrence King Federal Building is a multi-story steel framed structure with concrete masonry unit exterior walls. Construction was completed in 1993. The building is made up of approximately 263,355 total square feet and consists of 12 floors. The interior walls are gypsum wallboard. The floors are carpet throughout with vinyl floor tiles in some areas. Ceilings consist of tile panels on a suspended metal grid in most areas and are gypsum wallboard in other areas.

Two previous inspections found suspect biological growth on the blower wheels, side walls and gaskets in almost all the units. Heavy dust accumulation was observed in the supply duct and clumps of dust were observed in the HVAC supply registers.

In a conference call with representatives of the Office of Environmental Health and Occupational Safety it was recommended that until the problems with the air handlers can be corrected, air monitoring of half of the floors of the building should be conducted every six months.

During the survey, two air samples for mold were collected on each floor that was inspected as well as two from outside. None of the samples exceeded the lowest outdoor spore count. The measurements for all indoor air quality parameters were within the ASHRAE recommended guidelines on the day of the survey. It must be noted that many of the building occupants were on working from home due to the COVID-19 Pandemic and may not be representative of the temperatures and relative humidity usually experienced in the Miami area. Therefore, the indoor air quality measurements for carbon dioxide and relative humidity may not be reflective of usual building conditions.

Two tape lift samples were collected during the site visit, one from debris in the 2nd Floor Litigation Services the other from debris in the 7th Floor Asset Forfeiture Office. Both samples had “High” levels of Cladosporium. Cladosporium is a common spore that is often associated with dust and dust accumulation. Even though there was dust debris in these two areas there was no elevation in the air samples collected.

Key recommendations generated as a result of this survey include:

• During the previous two inspections air handling units for each floor were inspected.

Suspect biological growth was observed on the blower wheels, side walls and gaskets in almost all the units. Heavy dust accumulation was observed in the supply duct and clumps of dust were observed in the HVAC supply registers. It is recommended that the units and associated supply ducts be cleaned by a professional HVAC contractor.

• It is understood that correcting the issues with the duct work may take some time in the interim it is recommended that the biannual air monitoring continue.

2.0 INTRODUCTION

On May 13, 2020, Mr. Matthew Berry, representing the U.S. Public Health Service, Federal Occupational Health, conducted an indoor air quality monitoring which included collecting fungal air samples, and recording levels of carbon dioxide, carbon monoxide, temperature and relative humidity on floors 2, 3, 4, 6, 7 and 8 in the James Lawrence King Federal Building located at 99 NE 4th Street in Miami, Florida (GSA Building Number: FL1021). This biannual monitoring was requested in response to employee concerns related to suspected mold growth in the space. The air monitoring will be conducted every six months.

The James Lawrence King Federal Building is a multi-story steel framed structure with concrete masonry unit exterior walls. Construction was completed in 1993. The building is made up of approximately 263,355 total square feet and consists of 12 floors. The interior walls are gypsum wallboard. The floors are carpet throughout with vinyl floor tiles in some areas. Ceilings consist of tile panels on a suspended metal grid in most areas and are gypsum wallboard in other areas.

3.0 INSTRUMENTATION/CALIBRATION

The indoor air quality investigation included air quality measurements, moisture measurements and a visual inspection. Air quality parameters tested included:

• relative humidity

• temperature

• carbon dioxide (CO2)

• carbon monoxide (CO)

Air measurements for CO2, CO, relative humidity and temperature were collected using a direct read TSI 7575-X Q-Track IAQ meter. Moisture measurements were collected using a Protimeter moister meter. Fungal air samples were collected using Air-O-Cell cassettes and a Bio-Pump Plus pump.

4.0 RESULTS AND DISCUSSION

4.1 Heating, Ventilating and Air Conditioning (HVAC) Systems

Ventilation (the introduction of outdoor air) for the subject areas is provided by air handling units located on the roof for the 10th thru 12th floors, and individual units in mechanical spaces for the 1st thru 9th floors. The conditioned air is transported through externally lined duct. The return air for the HVAC units is provided through the ceiling plenum. The units were in good condition at the time of the inspection, however there was dust and particulate matter collecting within the ducts at the units and traveling out to the vents, indicating cleaning is necessary. The ducting was also in good, intact condition with no signs of breaches or deterioration.

4.2 Indoor Air Quality Guidelines

There are no government standards currently in effect for indoor air quality contaminants in the non-industrial and non-construction environment. A summary of the relevant guidelines to the work performed at the Site is presented below. Although not directly applicable to indoor air quality, regulatory standards for air contaminants are presented for comparison purposes.

Carbon Dioxide (CO2)

Carbon dioxide monitoring is a useful screening technique (non-quantitative) for determining if outside air supply is sufficient for maintaining acceptable indoor air quality.

Carbon dioxide is a naturally occurring constituent of the atmosphere and is also a product of human respiration. During periods of occupancy, carbon dioxide levels in a building will rise above the normal background level of approximately 320-350 parts per million (ppm).

The level of increase of carbon dioxide concentrations is directly related to the number of people in an area and the amount of outside air being introduced.

The American Society of Heating, Refrigeration and Air Conditioning Engineers (ASHRAE) has recommended a maximum carbon dioxide concentration of 700 ppm above background levels (approximately 1,000 ppm total) to maintain human comfort and odor control with a general recommended concentration for carbon dioxide in office space of 520 ppm above background (approximately 850 ppm total). To maintain acceptable indoor air quality in general office space, ASHRAE Standard 62.1-2010 recommends a minimum outside air supply of 5 cubic feet per minute (cfm) per person and .06 cfm per square foot of office space.

This guideline is only intended to result in indoor air quality acceptable to 80% of the building population. Conversely, exceeding this guideline does not necessarily indicate that an IAQ problem exists. However, the potential for complaints may increase depending on air contaminant source strengths and other factors.

Temperature and Relative Humidity (T/RH)

Temperature and humidity play important roles in indoor air quality. It has been suggested that occupant perceptions of air quality can rapidly decline when T/RH fall outside recommended comfort ranges. High temperature and humidity can also be conducive to the growth of many microbes, including fungi. ASHRAE standards from 2004 have recommended that the levels of these variables be maintained within a “comfort zone”.

(See Table 1b).

ASHRAE Standard 55-2004 describes six primary factors for the establishment of thermal comfort: the metabolic rate of occupants, occupant clothing insulation, air temperature, radiant temperature, air speed, and humidity. As these primary factors all influence thermal comfort, specific ranges of acceptable relative humidity values are not available.

4.3 Air Quality Results

Visual Inspection

Limited visual inspection was completed during the air sampling survey particulate matter was observed on horizontal surfaces in a few locations as pointed out by Ian Larson the building manager. The same matter was also observed at vents, trapped in the vent covers.

It was observed that in areas where they have had historical issues with the duct debris there was fibrous pre filter placed over the retuns.

Plants were observed frequently throughout areas, and some were not well-kept.

Overwatering, staining, spillage, or some combination thereof was observed around the plants. Some plants had microbial growth in the dirt or on the dead portions of the plant.

Dust was observed collecting on horizontal surfaces in multiple areas throughout the building.

Indoor Air Quality Parameters Testing

The results of indoor air quality parameter testing performed on May 13, 2020 using direct read instrumentation are compiled in the attached Table 1.

Temperature and relative humidity measurements at the time of the site visit were within the recommended range, in accordance with American Society for Heating, Refrigerating and Air-conditioning Engineers (ASHRAE) in Standards 55 & 62. The interior temperature ranged from 71 to 73°F. The relative humidity for the interior ranged from 49 to 61%.

Carbon dioxide (CO2) concentrations, often used as an indicator of the adequacy of ventilation, ranged from 415 to 610 parts per million (ppm) within the building during the monitoring period (See attached Attachment A for CO2 results and monitoring locations).

Based on this data, the building systems appeared to be introducing sufficient outdoor air for the building’s population on the monitored day. ASHRAE Standard 62.1-2016 recommends that ventilation systems servicing an office environment continually introduce a minimum amount of outdoor air based on space population and the square footage of the occupied space. Generally speaking, the calculated amount of recommended outdoor air introduction is between 17 and 20 cubic feet per minute (CFM) per occupant.

Appendix D of ASHARE 62.1-2016 offers a means to estimate the outdoor air introduction by observing the trending of indoor occupant generated CO2 concentrations over the workday. Provided indoor CO2 concentrations do not exceed 700 ppm above ambient (outdoor) concentrations (delta 700), the ventilation system is most likely introducing approximately 15 CFM per occupant. The average ambient carbon dioxide concentration during the May 13, 2020 monitoring period was 390 ppm, and per ASHRAE 62 (Appendix D), inside CO2 concentrations should not exceed 1,090 ppm if approximately 15 CFM of outdoor air per person is introduced.

The carbon monoxide (CO) measurements taken from inside the building were within recommended guidelines; the measurements ranged from below the detection limit of the device to 0.6 ppm. The outside CO reading ranged from 0.9 to 1.0 ppm. The EPA National Ambient Air Quality Standard (outside air) is 3.2 ppm and the Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit is 50 ppm.

Fungal Air Sampling Testing

14 Air-O-Cell cassette air samples as part of this investigation. There are no government standards currently in effect for microbiological contaminants. As recommended by the American Industrial Hygiene Association (AIHA) and American Conference of Governmental Industrial Hygienists (ACGIH), interpretation of fungal air sampling is generally limited to the comparison of indoor air sampling to outdoor air sampling results.

Indoor fungal aerosol concentrations significantly higher than outdoor fungal aerosol concentrations indicate that indoor mold growth may be occurring. If air sampling confirms the presence of one or more fungal genera indoors and not similarly present in outdoor samples, it suggests that there may be a reservoir of fungal growth indoors, especially if the fungal concentrations are high. This condition is also termed amplification.

It must be emphasized that fungal contamination is a dynamic phenomenon and that species within an area can change due to environmental conditions. Individual health may be affected by the presence of high levels of non-toxic or non-pathogenic microbes that may emit volatile compounds (musty odors) from the mycelia (fungal plant) or vapors from substrate breakdown.

The Air-O-Cell cassette is a unique sampling device designed for the rapid collection and analysis of a wide range of airborne particles, including fungal spores. Samples are collected by drawing air at a known rate of flow across a sticky glass slide, which is subsequently removed from the cassette in the analytical laboratory and analyzed via light microscopy at the standardized magnification of 600X. The results are reported as total amount seen, meaning they include both viable (live) and non-viable (dead) fungal spores.

Unfortunately, this technique does not allow for the differentiation between Aspergillus and Penicillium spores because they are morphologically identical. Additionally, the technique does not allow for cultivation, or the identification of spores to the species level, except in a few cases.

Non-regulatory guidelines based on extensive field experience are used to quantitatively evaluate sample results. Total mold spore counts from samples collected inside the building that are below ambient levels or below 2,000 spores/m3, whichever is greater, and less than 650 spores/m3 for individual mold organisms (such as Aspergillus, Penicillium, Aureobasidium, Chaetomium, Fusarium, Trichoderma, Stachybotrys, Ulocladium), are considered normal for the screening method utilized.

The air samples were collected from the north and south sides of floors 2, 3, 4, 6, 7 and 8 and two from outside. The indoor spore counts are listed in Table 1 below. The ambient sample count was 820 spores/m3 in the morning and 630 spores/m3 in the afternoon. The samples collected inside the building are acceptable relative to the standards for clean indoor environments.

Location Total spore count (spores/m3)

Elevated individual species and count

(spores/m3) Outside AM 820 N/A

2nd Floor South- Litigation Services 170 N/A 2nd Floor North- Mail Room 10 N/A

3rd Floor South- Civil 140 N/A 3rd Floor North- FLU 50 N/A

4th Floor South-ECO Crimes 150 N/A 4th Floor North- ENV Crimes 110 N/A 6th Floor South- Major Crimes 140 N/A 6th Floor North- Major Crimes 110 N/A

7th Floor South- Asset Forfeiture 60 N/A 7th Floor North- Narcotics 10 N/A

8th Floor South- U.S. Attorney 50 N/A 8th Floor North- National Security 40 N/A

Outside PM 630 N/A

Fungal Tape Lift Sampling

Two tape lift samples were collected during the site visit, one from debris in the 2nd Floor Litigation Services the other from debris in the 7th Floor Asset Forfeiture Office. Both samples had “High” levels of Cladosporium. Cladosporium is a common spore that is often associated with dust and dust accumulation.

Results of the tape lift samples indicated the presence of fungal organisms. The table below details the sample location, number, spore type, and relative concentration found in the samples.

Sample Location Spore Type Relative Concentration

2nd Floor Litigation Services Cladosporium High

7th Floor Asset Forfeiture Office Cladosporium High

5.0 CONCLUSIONS AND RECOMMENDATIONS

The indoor air quality readings of temperature, relative humidity, carbon dioxide and carbon monoxide were within the recommended guidelines for the respective constituents at the time of our testing activities.

Based on these results and our visual inspection the following recommendations are provided:

• Air handling units for each floor were inspected. Suspect biological growth was observed on the blower wheels, side walls and gaskets in almost all of the units. Heavy dust accumulation was observed in the supply duct and clumps of dust were observed in the HVAC supply registers. It is recommended that the units and associated supply ducts be cleaned by a professional HVAC contractor and placed on a regular cleaning and maintenance schedule.

• Plants were observed frequently throughout the building, and some were not well-kept.

Overwatering, staining, spillage, or some combination thereof was observed around the plants. Some plants had microbial growth in the dirt or on the dead portions of the plant. It is recommended that all plants be removed from the occupied space.

• Until the HVAC units and associated ducts can be replaced or repaired continue the biannual air monitoring.

7.0 SUPPORTING DOCUMENTS

The following supporting documents are attached to the report:

Attachments: A – IAQ Testing & Results B – Photographs C – Laboratory Report and Chain of Custody

ATTACHMENT A

IAQ TESTING & RESULTS

Sample Location Temperature1

(°F)

Relative Humidity1

Carbon Monoxide1

(ppm)

Carbon Dioxide1

(ppm)

Outside AM 79 60 1.0 388 2nd Floor South- Litigation Services 73 58 0.0 458

2nd Floor North- Mail Room 72 61 0.0 445 3rd Floor South- Civil 71 54 0.1 447 3rd Floor North- FLU 71 53 0.6 471

4th Floor South-ECO Crimes 72 58 0.2 610 4th Floor North- ENV Crimes 72 56 0.6 450 6th Floor South- Major Crimes 72 51 0.5 441 6th Floor North- Major Crimes 72 51 0.5 430

7th Floor South- Asset Forfeiture 71 57 0.4 463 7th Floor North- Narcotics 70 59 0.4 486

8th Floor South- U.S. Attorney 73 49 0.5 417 8th Floor North- National Security 73 49 0.6 415

Outside PM 81 62 0.9 391

Heating Season: 68-75 3 & 4

Cooling Season: 73-79 3 & 4 30 – 60 3 &

4 < 9 3 & 4 < 700 above outdoor level of 389 (<1,089)3 & 4

Notes:

1. Monitoring Instrument: TSI IAQ- CALC 7545 - Calibration due 5/2018

2. Guidance values from the American Industrial Hygiene Association (AIHA) Indoor Air Quality

Investigator’s Guide dated 2006

3. Guidance values from the American Society of Heating, Refrigerating and Air-conditioning

Engineers (ASHRAE)

4. U.S Green Building Council (USGBC) LEED on Indoor Air Quality (particles of ≤10 microns in diameter, or respirable particulate) Abbreviations:

ppm = parts per million mg/m3 = milligrams per cubic meter of air oF = degrees Fahrenheit

% = percent < = less than Hr = Hour

ATTACHMENT B

PHOTOGRAPHS

Front view of the James Lawrence King Federal Building, Miami, Florida.

Cubicle area in the 2nd Floor South Litigation Services.

T-1 collected behind the computer in the cubicle area in the 2nd Floor South Litigation Services.

Window sill in the 7th Floor South Asset Forfeiture.

T-2 collected from the windowsill in the 7th Floor South Asset Forfeiture

Numerous potted plants in the sitting area of 7th Floor South Asset Forfeiture.

http://www.EMSL.com / orlandolab@emsl.com

Tel/Fax: (407) 599-5887 / (407) 599-9063

3303 PARKWAY CENTER COURT Orlando, FL 32808

EMSL Analytical, Inc. EMSL Order: 342006887

Customer ID: EFIG62

Customer PO:

Project ID:

Matthew BerryAttention: Phone: (321) 251-9091

EFI Global, Inc. Fax: (321) 251-9099

11602 Lake Underhill Road Collected Date: 05/13/2020

Suite 140 Received Date: 05/14/2020 10:02 AM

Orlando, FL 32825 Analyzed Date: 05/15/2020

Project: 020.01097

Test Report:Air-O-Cell(™) Analysis of Fungal Spores & Particulates by Optical Microscopy (Methods MICRO-SOP-201, ASTM D7391)

Lab Sample Number:

Client Sample ID:

Volume (L):

Sample Location:

342006887-0001

A-1

342006887-0002

A-2

342006887-0003

A-3

Outside 2nd Floor South 2nd Floor North

Spore Types Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total

Alternaria (Ulocladium) - - - - - - - - -

Ascospores 10 420 51.2 - - - - - -

Aspergillus/Penicillium 5 200 24.4 - - - - - -

Basidiospores 3 100 12.2 - - - - - -

Bipolaris++ - - - - - - - - -

Chaetomium - - - - - - - - -

Cladosporium 3 100 12.2 2 80 47.1 - - -

Curvularia - - - 1 40 23.5 1* 10* 100

Epicoccum - - - - - - - - -

Fusarium - - - - - - - - -

Ganoderma - - - - - - - - -

Myxomycetes++ - - - 1* 10* 5.9 - - -

Pithomyces++ - - - - - - - - -

Rust - - - - - - - - -

Scopulariopsis/Microascus - - - - - - - - -

Stachybotrys/Memnoniella - - - - - - - - -

Unidentifiable Spores - - - 1 40 23.5 - - -

Zygomycetes - - - - - - - - -

Total Fungi 21 820 100 5 170 100 1 10 100

Hyphal Fragment 2 80 - 1* 10* - 1 40 -

Insect Fragment - - - - - - - - -

Pollen 1 40 - - - - - - -

Analyt. Sensitivity 600x - 42 - - 42 - - 42 -

Analyt. Sensitivity 300x - 13* - - 13* - - 13* -

Skin Fragments (1-4) - 1 - - 1 - - 2 -

Fibrous Particulate (1-4) - 1 - - 2 - - 1 -

Background (1-5) - 2 - - 2 - - 1 -

++ Includes other spores with similar morphology; see EMSL's fungal glossary for each specific category.

Yessica Martinez Seeman, Microbiology Technical Manager, Central Florida or other Approved SignatoryNo discernable field blank was submitted with this group of samples.

High levels of background particulate can obscure spores and other particulates, leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber particle or insect fragment. "*"

Denotes particles found at 300X. "-" Denotes not detected. Due to method stopping rules, raw counts in excess of 100 are extrapolated based on the percentage analyzed. EMSL maintains liability limited to cost of analysis.

Interpretation and use of test results are the responsibility of the client. This report relates only to the samples reported above, and may not be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. The report reflects the samples as received. When the information supplied by the customer can affect the validity of the result, it will be noted on the report.

Samples analyzed by EMSL Analytical, Inc. Orlando, FL AIHA-LAP, LLC EMLAP 163563

Initial report from: 05/15/2020 09:06 AM

For information on the fungi listed in this report, please visit the Resources section at www.emsl.com

MIC_M001_0002_0002 Printed: 05/15/2020 09:06 AM Page 1 of 5

3303 PARKWAY CENTER COURT Orlando, FL 32808

EMSL Analytical, Inc. EMSL Order: 342006887

Customer ID: EFIG62

Customer PO:

Project ID:

Matthew BerryAttention: Phone: (321) 251-9091

EFI Global, Inc. Fax: (321) 251-9099

11602 Lake Underhill Road Collected Date: 05/13/2020

Suite 140 Received Date: 05/14/2020 10:02 AM

Orlando, FL 32825 Analyzed Date: 05/15/2020

Project: 020.01097

Test Report:Air-O-Cell(™) Analysis of Fungal Spores & Particulates by Optical Microscopy (Methods MICRO-SOP-201, ASTM D7391)

Lab Sample Number:

Client Sample ID:

Volume (L):

Sample Location:

342006887-0004

A-4

342006887-0005

A-5

342006887-0006

A-6

3rd Floor South 3rd Floor North 4th Floor South

Spore Types Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total

Alternaria (Ulocladium) - - - 1* 10* 20 - - -

Ascospores - - - 1* 10* 20 1 40 26.7

Aspergillus/Penicillium 3 100 71.4 - - - - - -

Basidiospores - - - 1* 10* 20 - - -

Bipolaris++ - - - - - - - - -

Chaetomium - - - - - - - - -

Cladosporium - - - 1* 10* 20 - - -

Curvularia 1 40 28.6 - - - 1 40 26.7

Epicoccum - - - - - - 2* 30* 20

Fusarium - - - - - - - - -

Ganoderma - - - - - - - - -

Myxomycetes++ - - - - - - 1 40 26.7

Pithomyces++ - - - 1* 10* 20 - - -

Rust - - - - - - - - -

Scopulariopsis/Microascus - - - - - - - - -

Stachybotrys/Memnoniella - - - - - - - - -

Unidentifiable Spores - - - - - - - - -

Zygomycetes - - - - - - - - -

Total Fungi 4 140 100 5 50 100 5 150 100

Hyphal Fragment 2 80 - 1 40 - 2 80 -

Insect Fragment - - - - - - - - -

Pollen 1 40 - - - - 1* 10* -

Analyt. Sensitivity 600x - 42 - - 42 - - 42 -

Analyt. Sensitivity 300x - 13* - - 13* - - 13* -

Skin Fragments (1-4) - 2 - - 2 - - 2 -

Fibrous Particulate (1-4) - 1 - - 2 - - 2 -

Background (1-5) - 2 - - 2 - - 2 -

++ Includes other spores with similar morphology; see EMSL's fungal glossary for each specific category.

Yessica Martinez Seeman, Microbiology Technical Manager, Central Florida or other Approved SignatoryNo discernable field blank was submitted with this group of samples.

High levels of background particulate can obscure spores and other particulates, leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber particle or insect fragment. "*"

Denotes particles found at 300X. "-" Denotes not detected. Due to method stopping rules, raw counts in excess of 100 are extrapolated based on the percentage analyzed. EMSL maintains liability limited to cost of analysis.

Interpretation and use of test results are the responsibility of the client. This report relates only to the samples reported above, and may not be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. The report reflects the samples as received. When the information supplied by the customer can affect the validity of the result, it will be noted on the report.

Samples analyzed by EMSL Analytical, Inc. Orlando, FL AIHA-LAP, LLC EMLAP 163563

Initial report from: 05/15/2020 09:06 AM

For information on the fungi listed in this report, please visit the Resources section at www.emsl.com

MIC_M001_0002_0002 Printed: 05/15/2020 09:06 AM Page 2 of 5

3303 PARKWAY CENTER COURT Orlando, FL 32808

EMSL Analytical, Inc. EMSL Order: 342006887

Customer ID: EFIG62

Customer PO:

Project ID:

Matthew BerryAttention: Phone: (321) 251-9091

EFI Global, Inc. Fax: (321) 251-9099

11602 Lake Underhill Road Collected Date: 05/13/2020

Suite 140 Received Date: 05/14/2020 10:02 AM

Orlando, FL 32825 Analyzed Date: 05/15/2020

Project: 020.01097

Test Report:Air-O-Cell(™) Analysis of Fungal Spores & Particulates by Optical Microscopy (Methods MICRO-SOP-201, ASTM D7391)

Lab Sample Number:

Client Sample ID:

Volume (L):

Sample Location:

342006887-0007

A-7

342006887-0008

A-8

342006887-0009

A-9

4th Floor North 6th Floor South 6th Floor North

Spore Types Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total

Alternaria (Ulocladium) - - - - - - - - -

Ascospores 1* 10* 9.1 - - - 1* 10* 9.1

Aspergillus/Penicillium 3 100 90.9 7* 90* 64.3 1* 10* 9.1

Basidiospores - - - - - - 1* 10* 9.1

Bipolaris++ - - - - - - - - -

Chaetomium - - - - - - - - -

Cladosporium - - - - - - - - -

Curvularia - - - 1* 10* 7.1 - - -

Epicoccum - - - - - - - - -

Fusarium - - - - - - - - -

Ganoderma - - - - - - - - -

Myxomycetes++ - - - 1 40 28.6 2 80 72.7

Pithomyces++ - - - - - - - - -

Rust - - - - - - - - -

Scopulariopsis/Microascus - - - - - - - - -

Stachybotrys/Memnoniella - - - - - - - - -

Unidentifiable Spores - - - - - - - - -

Zygomycetes - - - - - - - - -

Total Fungi 4 110 100 9 140 100 5 110 100

Hyphal Fragment 2 80 - 2 80 - - - -

Insect Fragment 3* 40* - - - - - - -

Pollen - - - - - - - - -

Analyt. Sensitivity 600x - 42 - - 42 - - 42 -

Analyt. Sensitivity 300x - 13* - - 13* - - 13* -

Skin Fragments (1-4) - 2 - - 2 - - 2 -

Fibrous Particulate (1-4) - 2 - - 1 - - 2 -

Background (1-5) - 2 - - 2 - - 2 -

++ Includes other spores with similar morphology; see EMSL's fungal glossary for each specific category.

Yessica Martinez Seeman, Microbiology Technical Manager, Central Florida or other Approved SignatoryNo discernable field blank was submitted with this group of samples.

High levels of background particulate can obscure spores and other particulates, leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber particle or insect fragment. "*"

Denotes particles found at 300X. "-" Denotes not detected. Due to method stopping rules, raw counts in excess of 100 are extrapolated based on the percentage analyzed. EMSL maintains liability limited to cost of analysis.

Interpretation and use of test results are the responsibility of the client. This report relates only to the samples reported above, and may not be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. The report reflects the samples as received. When the information supplied by the customer can affect the validity of the result, it will be noted on the report.

Samples analyzed by EMSL Analytical, Inc. Orlando, FL AIHA-LAP, LLC EMLAP 163563

Initial report from: 05/15/2020 09:06 AM

For information on the fungi listed in this report, please visit the Resources section at www.emsl.com

MIC_M001_0002_0002 Printed: 05/15/2020 09:06 AM Page 3 of 5

3303 PARKWAY CENTER COURT Orlando, FL 32808

EMSL Analytical, Inc. EMSL Order: 342006887

Customer ID: EFIG62

Customer PO:

Project ID:

Matthew BerryAttention: Phone: (321) 251-9091

EFI Global, Inc. Fax: (321) 251-9099

11602 Lake Underhill Road Collected Date: 05/13/2020

Suite 140 Received Date: 05/14/2020 10:02 AM

Orlando, FL 32825 Analyzed Date: 05/15/2020

Project: 020.01097

Test Report:Air-O-Cell(™) Analysis of Fungal Spores & Particulates by Optical Microscopy (Methods MICRO-SOP-201, ASTM D7391)

Lab Sample Number:

Client Sample ID:

Volume (L):

Sample Location:

342006887-0010

A-10

342006887-0011

A-11

342006887-0012

A-12

7th Floor South 7th Floor North 8th Floor South

Spore Types Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total

Alternaria (Ulocladium) - - - - - - - - -

Ascospores - - - - - - - - -

Aspergillus/Penicillium - - - - - - 1* 10* 20

Basidiospores 1 40 66.7 - - - - - -

Bipolaris++ - - - - - - - - -

Chaetomium - - - - - - - - -

Cladosporium 1* 10* 16.7 1* 10* 100 - - -

Curvularia - - - - - - - - -

Epicoccum - - - - - - - - -

Fusarium - - - - - - - - -

Ganoderma - - - - - - - - -

Myxomycetes++ 1* 10* 16.7 - - - 1 40 80

Pithomyces++ - - - - - - - - -

Rust - - - - - - - - -

Scopulariopsis/Microascus - - - - - - - - -

Stachybotrys/Memnoniella - - - - - - - - -

Unidentifiable Spores - - - - - - - - -

Zygomycetes - - - - - - - - -

Total Fungi 3 60 100 1 10 100 2 50 100

Hyphal Fragment 2* 30* - 2 80 - 1 40 -

Insect Fragment - - - - - - - - -

Pollen - - - 1 40 - - - -

Analyt. Sensitivity 600x - 42 - - 42 - - 42 -

Analyt. Sensitivity 300x - 13* - - 13* - - 13* -

Skin Fragments (1-4) - 2 - - 2 - - 1 -

Fibrous Particulate (1-4) - 1 - - 1 - - 1 -

Background (1-5) - 2 - - 2 - - 2 -

++ Includes other spores with similar morphology; see EMSL's fungal glossary for each specific category.

Yessica Martinez Seeman, Microbiology Technical Manager, Central Florida or other Approved SignatoryNo discernable field blank was submitted with this group of samples.

High levels of background particulate can obscure spores and other particulates, leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber particle or insect fragment. "*"

Denotes particles found at 300X. "-" Denotes not detected. Due to method stopping rules, raw counts in excess of 100 are extrapolated based on the percentage analyzed. EMSL maintains liability limited to cost of analysis.

Interpretation and use of test results are the responsibility of the client. This report relates only to the samples reported above, and may not be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. The report reflects the samples as received. When the information supplied by the customer can affect the validity of the result, it will be noted on the report.

Samples analyzed by EMSL Analytical, Inc. Orlando, FL AIHA-LAP, LLC EMLAP 163563

Initial report from: 05/15/2020 09:06 AM

For information on the fungi listed in this report, please visit the Resources section at www.emsl.com

MIC_M001_0002_0002 Printed: 05/15/2020 09:06 AM Page 4 of 5

3303 PARKWAY CENTER COURT Orlando, FL 32808

EMSL Analytical, Inc. EMSL Order: 342006887

Customer ID: EFIG62

Customer PO:

Project ID:

Matthew BerryAttention: Phone: (321) 251-9091

EFI Global, Inc. Fax: (321) 251-9099

11602 Lake Underhill Road Collected Date: 05/13/2020

Suite 140 Received Date: 05/14/2020 10:02 AM

Orlando, FL 32825 Analyzed Date: 05/15/2020

Project: 020.01097

Test Report:Air-O-Cell(™) Analysis of Fungal Spores & Particulates by Optical Microscopy (Methods MICRO-SOP-201, ASTM D7391)

Lab Sample Number:

Client Sample ID:

Volume (L):

Sample Location:

342006887-0013

A-13

342006887-0014

A-14

342006887-9901

Dummy

8th Floor North Outside Dummy

Spore Types Raw Count Count/M³ % of Total Raw Count Count/M³ % of Total - - -

Alternaria (Ulocladium) - - - - - - - - -

Ascospores - - - 6 300 47.6 - - -

Aspergillus/Penicillium 1 40 100 5 200 31.7 - - -

Basidiospores - - - 2 80 12.7 - - -

Bipolaris++ - - - - - - - - -

Chaetomium - - - - - - - - -

Cladosporium - - - 1* 10* 1.6 - - -

Curvularia - - - - - - - - -

Epicoccum - - - - - - - - -

Fusarium - - - - - - - - -

Ganoderma - - - - - - - - -

Myxomycetes++ - - - - - - - - -

Pithomyces++ - - - - - - - - -

Rust - - - - - - - - -

Scopulariopsis/Microascus - - - - - - - - -

Stachybotrys/Memnoniella - - - - - - - - -

Unidentifiable Spores - - - 1 40 6.3 - - -

Zygomycetes - - - - - - - - -

Total Fungi 1 40 100 15 630 100 - - -

Hyphal Fragment - - - - - - - - -

Insect Fragment - - - - - - - - -

Pollen 1 40 - 2* 30* - - - -

Analyt. Sensitivity 600x - 42 - - 42 - - - -

Analyt. Sensitivity 300x - 13* - - 13* - - - -

Skin Fragments (1-4) - 1 - - 1 - - - -

Fibrous Particulate (1-4) - 1 - - 1 - - - -

Background (1-5) - 3 - - 2 - - - -

++ Includes other spores with similar morphology; see EMSL's fungal glossary for each specific category.

Yessica Martinez Seeman, Microbiology Technical Manager, Central Florida or other Approved SignatoryNo discernable field blank was submitted with this group of samples.

High levels of background particulate can obscure spores and other particulates, leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber particle or insect fragment. "*"

Denotes particles found at 300X. "-" Denotes not detected. Due to method stopping rules, raw counts in excess of 100 are extrapolated based on the percentage analyzed. EMSL maintains liability limited to cost of analysis.

Interpretation and use of test results are the responsibility of the client. This report relates only to the samples reported above, and may not be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. The report reflects the samples as received. When the information supplied by the customer can affect the validity of the result, it will be noted on the report.

Samples analyzed by EMSL Analytical, Inc. Orlando, FL AIHA-LAP, LLC EMLAP 163563

Initial report from: 05/15/2020 09:06 AM

For information on the fungi listed in this report, please visit the Resources section at www.emsl.com

MIC_M001_0002_0002 Printed: 05/15/2020 09:06 AM Page 5 of 5

3303 PARKWAY CENTER COURT Orlando, FL 32808

EMSL Analytical, Inc. EMSL Order: 342006887

Customer ID: EFIG62

Customer PO:

Project ID:

Attention: (321) 251-9091Phone: Matthew Berry

EFI Global, Inc. (321) 251-9099Fax:

11602 Lake Underhill Road Collected Date: 05/13/2020

Suite 140 Received Date: 05/14/2020

Orlando, FL 32825 Analyzed Date: 05/15/2020

Project: 020.01097

Test Report: Microscopic Examination of Fungal Spores, Fungal Structures, Hyphae, and Other Particulates from Tape Samples (EMSL Method MICRO-SOP-200)

Lab Sample Number:

Client Sample ID:

Sample Location:

342006887-0015

T-1

342006887-0016

T-2

342006887-9901

Dummy

342006887-9902

Dummy

342006887-9903

Dummy

2nd Floor Lit Service 7th Floor Asst Dummy Dummy Dummy

Spore Types Category Category - - -

Alternaria (Ulocladium) - - - - -

Ascospores - - - - -

Aspergillus/Penicillium - - - - -

Basidiospores - - - - -

Bipolaris++ - - - - -

Chaetomium - - - - -

Cladosporium *High* High - - -

Curvularia - - - - -

Epicoccum - - - - -

Fusarium - - - - -

Ganoderma - - - - -

Myxomycetes++ - - - - -

Pithomyces++ - - - - -

Rust - - - - -

Scopulariopsis/Microascus - - - - -

Stachybotrys/Memnoniella - - - - -

Unidentifiable Spores - - - - -

Zygomycetes - - - - -

Hyphal Fragment - - - - -

Insect Fragment - - - - -

Pollen - - - - -

Category: Count/per area analyzed - Rare: 1 to 10 Low: 11 to 100 Medium: 101 to 1000 High: >1000

- Denotes Not Detected.

++ Includes other spores with similar morphology; see EMSL's fungal glossary for each specific category.

* = Sample contains fruiting structures and/or hyphae associated with the spores.

Yessica Martinez Seeman, Microbiology Technical

Manager, Central Florida or other Approved SignatoryNo discernable field blank was submitted with this group of samples.

EMSL maintains liability limited to cost of analysis. Interpretation and use of test results are the responsibility of the client. This report relates only to the samples reported above and may not be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. The report reflects the samples as received. When the information supplied by the customer can affect the validity of the result, it will be noted on the report.

Samples analyzed by EMSL Analytical, Inc. Orlando, FL AIHA-LAP, LLC--EMLAP Accredited #163563

Initial report from: 05/15/2020 09:06 AM

For information on the fungi listed in this report, please visit the Resources section at www.emsl.com

Page 1 of 1Test Report DEVER1-2.9.0 Printed 05/15/2020 09:06 AM

OrderID: 342006887

OrderID: 342006887