Jonah Ventures, LLC

Jonah Ventures, LLC, a small business limited liability company based in Boulder, Colorado, provides environmental DNA (eDNA) sequencing, library preparation, and bioinformatic analysis services to federal research agencies as a prime contractor on small-business-set-aside and unrestricted purchase orders and blanket purchase agreements. The vendor competes across both set-aside and full-and-open categories, with 39% of recent awards designated as total small business set-asides and 61% awarded full-and-open.

Jonah Ventures specializes in concrete eDNA workflow services: DNA extraction from environmental samples (seawater, freshwater, fecal matter), PCR amplification using species-specific and multi-marker assays (12S MiFish, COI Leray, MarVer1, qPCR), high-throughput metabarcoding sequencing (Illumina and Oxford Nanopore platforms), and bioinformatic processing including taxonomic assignment, exact sequence variant calling, and quality control. Recent representative work includes a $96,768 total-small-business purchase order from NOAA's Pacific Marine Environmental Laboratory for FY26 eDNA library preparation and sequencing supporting oceanographic research (awarded September 2026, performance through September 2027); a $47,140 total-small-business purchase order from the U.S. Geological Survey for custom reference DNA library development and metabarcoding optimization using Nanopore sequencing of 200–300 environmental samples (awarded August 2026); and a $106,000 unrestricted purchase order from NOAA for eDNA extraction, amplification, and library preparation for amplicon sequencing under sole-source justification based on Jonah Ventures' methodological protocols and proven marine eDNA capability (awarded January 2025). The vendor also holds a five-year USGS blanket purchase agreement valued at $250,000 for on-demand eDNA metabarcoding analysis including macroinvertebrate community profiling and fecal source tracking (awarded February 2025, performance through February 2030), and a five-year USGS indefinite-delivery contract at $249,000 for library preparation and amplicon sequencing services (awarded April 2022, completion April 2027).

Customer concentration centers on two dominant agencies. NOAA accounts for 9 awards totaling $1.2 million in combined potential value (41% of the portfolio); USGS Office of Acquisitions and Grants accounts for 9 awards totaling $623 thousand (21% of potential value). Together, these two customers anchor 38% of all awards and 56% of combined potential value. Secondary customers include the Smithsonian Institution (5 awards, $314 thousand), USDA Animal and Plant Health Inspection Service Wildlife Services (5 awards, $515 thousand), USDA Agricultural Research Service (5 awards, $36 thousand), and USGS Alaska Science Center (6 awards, $104 thousand). The top five customer aggregations account for 74% of recent award count and 95% of combined potential value. NAICS coding is concentrated in Testing Laboratories and Services (541380, 37 awards); PSC coding reflects Special Studies/Analysis for Wildlife (12 awards) and Animal/Fisheries (10 awards).

Activity shows volatility year over year — 7 awards in 2022, 2 in 2023, 3 in 2024, 4 in 2025, and 3 in the first ten months of 2026 — with three awards totaling $177 thousand reaching ultimate completion inside the next twenty-four months and subject to recompete. Service Contract Inventory records are sparse (4 SCI-reportable records across FY2022–FY2025) and concentrated in FY2022–FY2023, reflecting Jonah Ventures' mix of high-touch, low-volume scientific services and goods that fall below the $150,000 SCI reporting threshold. Where SCI-reportable work was captured, invoiced amounts totaled $334 thousand across FY2022–FY2025 (FY2022: $116 thousand; FY2023: $218 thousand; FY2024–FY2025: $0). Government-paid hourly rates on SCI-reportable prime contracts ran a median of approximately $944.61/hr, with senior labor pricing reaching the 90th percentile at $1,277.83/hr. Jonah Ventures operates as a 100% prime contractor with no named subcontractor engagement; it performs all work with internal staff across laboratory and bioinformatic functions.

We have the capability to extract, amplify, and sequence DNA in a variety of samples using next generation sequencing. DNA can be analyzed to reconstruct animal diet, honey provenance, dust composition and origin.