DNA library prep & metabarcoding sequencing

Active Purchase Order Awarded Last action

Contract number
140G0126P0254
Awardee
Awarding agency
Obligated
$47,140

Award facts

Opportunity
Contract value 100% obligated
$47,140.00 Current and potential, with all options
Completion date 24% complete
Current and potential, with all options
Set-aside
Total Small Business
Competition
Competed under SAP · 2 offers Simplified Acquisition
Pricing
Firm Fixed Price
Place of performance
Boulder, CO 80301, USA
Legislative mandates

Award details come from SAM.gov/FPDS.

Transactions

Action Obligated Date
Other Administrative Action P00001 $0
Original award $47,140

About this award

The U.S. Geological Survey awarded a purchase order to Jonah Ventures, LLC, a small business, for DNA library preparation and metabarcoding sequencing services valued at $47,140.00 on August 4, 2026, under a total small business set-aside. Performance is located in Boulder, Colorado, with an ultimate completion date of August 9, 2027. The order covers processing of environmental DNA samples—approximately 200 to 300 samples across multiple batches—with development of a custom reference DNA library, optimization of a metabarcoding assay using pooled single-target PCR, and sequencing on Oxford Nanopore Promethion flow cells to achieve minimum 100,000 reads per sample. Deliverables include taxonomic assignments, exact sequence variants, fastq files, and quality control reports meeting publication-quality standards, delivered within six weeks of sample intake. The underlying solicitation was posted June 26, 2026, with quotes due July 2, 2026, and award on a lowest-price technically acceptable basis.

Quick answers

Who was awarded contract 140G0126P0254?

140G0126P0254 was awarded to Jonah Ventures, LLC by Office of Acquisitions and Grants (US Geological Survey) on August 4, 2026.

How much is contract 140G0126P0254 worth?

The government has obligated $47,140 on 140G0126P0254.

Was contract 140G0126P0254 competed?

The extent of competition for 140G0126P0254 was competed under sap. The government received 2 offers. It was set aside: Total Small Business.

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