Single Nucleotide Polymorphism Genotyping and Parentage Assignment Services in Rainbow Trout

The USDA Agricultural Research Service National Center for Cool and Cold Water Aquaculture is procuring Single Nucleotide Polymorphism Genotyping and Parentage Assignment Services in Rainbow Trout through an Indefinite Delivery Indefinite Quantity (IDIQ) contract. The selected contractor will be responsible for performing DNA extractions and SNP genotyping of rainbow trout DNA samples using low-density assays of at least 180 SNPs, with the objective of achieving high-confidence parentage assignments for at least 95% of offspring. Key performance requirements include extracting a minimum of 200 ng of total rainbow trout genome DNA per sample, achieving SNP call rates greater than 90%, maintaining a 95% sample pass rate for offspring and 100% for parents, and utilizing a fully integrated Laboratory Information Management System (LIMS) for sample tracking. The contractor must deliver genotyping data, parentage analysis results, and return unused tissue samples within 90 days of sample receipt. The solicitation was posted on July 10, 2025, with proposals due by July 22, 2025.

This procurement is designated as a 100% small business set-aside under NAICS code 541380 (Testing Laboratories and Services) with a business size standard of $20.5 million. The contract structure includes a base year with four option years, spanning from August 1, 2025, to July 31, 2030, with a total contract ceiling value of $500,000. No current contractors or incumbents are identified in the solicitation materials. The contract will be performed in Kearneysville, West Virginia, at the government facility. The solicitation document indicates this is a competitive procurement process designed to establish a multi-year testing services capability for the government's aquaculture research programs.