RNA-Seq of 140 Samples - RFQ
Closed Solicitation Posted
This opportunity was awarded. See the award notice from .
- Solicitation number
- 1232SA25Q0910
- Agency
- Agricultural Research Service Department of Agriculture
- Responses due
- Set-aside
- Total Small Business
Opportunity facts
- NAICS code
- 621511 Medical Laboratories
- Place of performance
- Beltsville, Maryland 20705, United States
- Points of contact
-
- Aaron Dimeo aaron.dimeo@usda.gov (608) 416-0027
Notice details come from SAM.gov. Updated .
About this opportunity
The Department of Agriculture Agricultural Research Service is seeking RNA-Seq sequencing services for 140 pig intestinal tissue samples through Request for Quotation (RFQ) #1232SA25Q0910. The procurement includes four distinct line items: assessing RNA quality for 140 samples, preparing sequencing libraries for 140 samples, pooling and normalizing libraries, and sequencing libraries to produce approximately 30 million paired-end reads per sample at 2 x 150 base pair length. Contractors must demonstrate capability to perform poly-A tail enrichment library construction while preserving RNA molecule directionality, conduct quality control assessments, and deliver FASTQ files along with comprehensive QC reports. The solicitation utilizes a Lowest Price Technically Acceptable (LPTA) evaluation methodology, selecting the lowest-priced quote that meets all technical requirements. Quotes are due September 25, 2025, with an amendment issued September 23, 2025, adding a questions and answers document for technical clarification.
This opportunity is designated as a 100% total small business set-aside under NAICS code 621511 (Medical Laboratories) with a size standard of $41.5 million. No incumbent contractors are identified in the solicitation documents. While no specific contract value is disclosed, the scope involves processing 140 individual RNA samples through a comprehensive multi-step sequencing workflow. All work must be completed within 270 days from contract award, with deliverables including digital transfer of raw sequence data and associated reports to ARS researchers. The place of performance is specified as Calverton, Maryland, with samples originating from the USDA Agricultural Research Service Animal Biosciences and Biotechnology Laboratory in Beltsville, Maryland.
Notice text
2 versions
Update #2 · Latest ·
AMENDMENT 0001 (Dated 09/23/2025):
This amendment (0001) is being issued to add a questions and answers document to the Attachments/Links section. All other terms and conditions remain unchanged.
i. This is a combined synopsis/solicitation for commercial items prepared in accordance with the procedures contained in FAR Part 12.6, Acquisition of Commercial Items, and FAR Part 13, Simplified Acquisition Procedures, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued.
ii. The solicitation number (#) is 1232SA25Q0910 and is issued as a Request for Quotation (RFQ).
iii. The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) Number 2025-05 dated 08/07/2025.
Service Contract Labor Standards Wage Rate Determination Number 2015-4281 for Prince George's County, Maryland is applicable and is attached.
iv. This solicitation is issued as a 100% total small business set aside. The associated NAICS code is 621511 the small business size standard is $41.5 Million
v. List CLINS, item descriptions, quantities and units of measure:
0001 - Assess RNA Quality; Qty: 140 Each (EA)
0002 - Prepare Sequencing Libraries; Qty: 140 Each (EA)
0003 - Pool and Normalize Libraries: 1 Each (EA)
0004 - Sequence Libraries; Qty: 1 Each (EA)
vi. Description/Performance Work Statment:
See Attachment 2.
vii. Date(s) of performance and acceptance:
Work to be completed within 270 days from award.
viii. FAR Provision 52.212-1, Instructions to Offerors-Commercial, is incorporated by reference and applies to this acquisition. Please see the attached RFQ terms and conditions for the submission requirements and its addendum.
ix. Evaluation:
For the evaluation criteria, please see the attached RFQ terms and conditions.
x. Quoters are required to complete in full FAR Provision 52.212-3, Offeror Representations and Certifications-Commercial Items (DEVIATION), with their quote online at SAM: www.sam.gov. A quoter must state in their quote if they completed FAR 52.212-3 online and that is it up-to-date and valid. See the attached RFQ terms and conditions for more requirements.
System updates may lag policy updates. The System for Award Management (SAM) may continue to require entities to complete representations based on provisions that are not included in agency solicitations, including 52.223-22, Public Disclosure of Greenhouse Gas Emissions and Reduction Goals—Representation, and paragraph (t) of 52.212-3, Offeror Representations and Certifications—Commercial Products and Commercial Services. Agencies will not consider or use these representations. Entities are not required to, nor are they able to, update their entity registration to remove these representations in SAM.
xi. FAR Clause 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. Please see the attached RFQ terms and conditions for its Addendum.
xii. FAR Clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items (DEVIATION), is incorporated and applies to this acquisition. Please see the attached RFQ terms and conditions that are applicable to the acquisition.
xiii. There are no additional contract requirement(s) or terms and conditions (such as contract financing arrangements or warranty requirements) determined by the contracting officer to be necessary for this acquisition and consistent with customary commercial practices.
xiv. The Defense Priorities and Allocations System (DPAS) and assigned ratings are not applicable to this solicitation.
xv. Date, time and place quotes are due.
Quotes are due by the date/time/time zone listed in the “Date Offers Due” field.
Submit quotes to the following e-mail address(es), by the quote due date and time:
aaron.dimeo@usda.gov.
Late quotes after the established deadline may be considered only if it is in the Government’s best interest and if it will not unduly delay award.
xvi. Any and all questions regarding this solicitation shall be submitted in writing to aaron.dimeo@usda.gov no later than 72-hours prior to the close date/time/time zone listed in the “Date Offers Due” field. Answers will be posted at least 48-hours prior to the “Date Offers Due.”
Update #1 ·
i. This is a combined synopsis/solicitation for commercial items prepared in accordance with the procedures contained in FAR Part 12.6, Acquisition of Commercial Items, and FAR Part 13, Simplified Acquisition Procedures, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued.
ii. The solicitation number (#) is 1232SA25Q0910 and is issued as a Request for Quotation (RFQ).
iii. The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) Number 2025-05 dated 08/07/2025.
Service Contract Labor Standards Wage Rate Determination Number 2015-4281 for Prince George's County, Maryland is applicable and is attached.
iv. This solicitation is issued as a 100% total small business set aside. The associated NAICS code is 621511 the small business size standard is $41.5 Million
v. List CLINS, item descriptions, quantities and units of measure:
0001 - Assess RNA Quality; Qty: 140 Each (EA)
0002 - Prepare Sequencing Libraries; Qty: 140 Each (EA)
0003 - Pool and Normalize Libraries: 1 Each (EA)
0004 - Sequence Libraries; Qty: 1 Each (EA)
vi. Description/Performance Work Statment:
See Attachment 2.
vii. Date(s) of performance and acceptance:
Work to be completed within 270 days from award.
viii. FAR Provision 52.212-1, Instructions to Offerors-Commercial, is incorporated by reference and applies to this acquisition. Please see the attached RFQ terms and conditions for the submission requirements and its addendum.
ix. Evaluation:
For the evaluation criteria, please see the attached RFQ terms and conditions.
x. Quoters are required to complete in full FAR Provision 52.212-3, Offeror Representations and Certifications-Commercial Items (DEVIATION), with their quote online at SAM: www.sam.gov. A quoter must state in their quote if they completed FAR 52.212-3 online and that is it up-to-date and valid. See the attached RFQ terms and conditions for more requirements.
System updates may lag policy updates. The System for Award Management (SAM) may continue to require entities to complete representations based on provisions that are not included in agency solicitations, including 52.223-22, Public Disclosure of Greenhouse Gas Emissions and Reduction Goals—Representation, and paragraph (t) of 52.212-3, Offeror Representations and Certifications—Commercial Products and Commercial Services. Agencies will not consider or use these representations. Entities are not required to, nor are they able to, update their entity registration to remove these representations in SAM.
xi. FAR Clause 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. Please see the attached RFQ terms and conditions for its Addendum.
xii. FAR Clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items (DEVIATION), is incorporated and applies to this acquisition. Please see the attached RFQ terms and conditions that are applicable to the acquisition.
xiii. There are no additional contract requirement(s) or terms and conditions (such as contract financing arrangements or warranty requirements) determined by the contracting officer to be necessary for this acquisition and consistent with customary commercial practices.
xiv. The Defense Priorities and Allocations System (DPAS) and assigned ratings are not applicable to this solicitation.
xv. Date, time and place quotes are due.
Quotes are due by the date/time/time zone listed in the “Date Offers Due” field.
Submit quotes to the following e-mail address(es), by the quote due date and time:
aaron.dimeo@usda.gov.
Late quotes after the established deadline may be considered only if it is in the Government’s best interest and if it will not unduly delay award.
xvi. Any and all questions regarding this solicitation shall be submitted in writing to aaron.dimeo@usda.gov no later than 72-hours prior to the close date/time/time zone listed in the “Date Offers Due” field. Answers will be posted at least 48-hours prior to the “Date Offers Due.”
Attachments
| File | Type | Posted |
|---|---|---|
| Questions and Answers for Sol 1232SA25Q0910.pdf | ||
| 1232SA25Q0910 - RFQ Terms and Conditions.pdf | ||
| Attachment 2 - Statement of Work (SOW).pdf | ||
| Attachment 1 - Quote Submission Form (QSF).pdf |
Notice history
| Notice | Type | Posted |
|---|---|---|
| RNA-Seq of 140 Samples - RFQ | Award Notice | |
| RNA-Seq of 140 Samples - RFQ | Solicitation |
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