Reagents kits for library preparation and sequencing
Closed Solicitation Posted
- Solicitation number
- VEID240013_1301160502
- Agency
-
Naval Supply Systems Command
Department of the Navy, Department of Defense
- Responses due
- Set-aside
- 8(a) Sole Source
Opportunity facts
- NAICS code
- 424210 Drugs and Druggists' Sundries Merchant Wholesalers
- Place of performance
- Bellavista Lima 01 Peru
Notice details come from SAM.gov. Updated .
About this opportunity
The Department of the Navy Naval Supply Systems Command is seeking to procure reagent kits for library preparation and sequencing using the Illumina MiSeq and MiniSeq platforms. The required kits must include steps for enzymatic fragmentation, size selection, end-repair, a-tailing, and ligation, allowing for dual indexing. The sequencing kits must support over 150 sequencing cycles to enable high-quality, high-throughput paired-end sequencing with over 2.0Gb per run and high Q30 values. The sequencing kits should also include an internal control. Proposals are due by June 20, 2024.
This opportunity is set aside for 8(a) small businesses. The required quantities include FC-110-3001 PhiX Control v3 kit - 3 quantities, MS-102-3003 MiSeq Reagent kit v3 (600-cycle) - 17 quantities, FC-131-1096 Nextera XT DNA Sample Preparation kit - 6 quantities, FC-131-2001 Nextera XT DNA Index kit - 4 quantities, and FC-420-1003 MiniSeq High Output Reagent Kit (300 cycles) - 9 quantities. The items must be delivered in two installments to the Callao International Airport in Bellavista, Peru.
Notice text
Reagents to prepare libraries using human and animal samples that later will be sequenced in the MiSeq and Miniseq platforms.
Kits must be designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime ends for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high-quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run.
- FC-110-3001 PhiX Control v3 kit - 3 quantities
- MS-102-3003 MiSeq Reagent kit v3 (600-cycle) - 17 quantities
- FC-131-1096 Nextera XT DNA Sample Preparation kit - 6 quantities
- FC-131-2001 Nextera XT DNA Index kit - 4 quantities
- FC-420-1003 MiniSeq High Output Reagent Kit (300 cycles) - 9 quantities
Items requested must be shipped to the Callao International Airport in two installments: first shipment must be executed upon award signature; and second one must be executed five months after that first shipment. This timeline is important since these reagents are very time-sensitive and have limited expiration dates.
Files
| File | Type | Posted |
|---|---|---|
| VEID240013_SOW.docx | DOCX document |
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