Multiomics Profiling

The United States Department of Agriculture Agricultural Research Service seeks integrated multiomics profiling services for 97 cattle samples, with quotations due July 27, 2026. The USDA intends to award on a single-source basis to Azenta US, Inc., though all responsible sources may submit quotations and the Government reserves discretion to compete based on responses received.

Place of performance is Calverton, MD 20705, with library preparation and sequencing to occur at the contractor's facility and delivery to the NEA Animal Genomics and Improvement facility in Beltsville, Maryland. The NAICS code is 541380, Testing Laboratories and Services, with a small business size standard of $19 million. This is an unrestricted acquisition.

Work comprises three sequencing components: 10X Genomics Chromium Multiome profiling of 25 samples targeting approximately 10,000 cells and 50,000 reads per cell to assess chromatin accessibility and gene expression; 10X Genomics Chromium 5' GEX with TCR/BCR enrichment of 12 samples targeting approximately 10,000 cells and 50,000 reads per cell to evaluate immune diversity; and metatranscriptomics sequencing of 60 samples including RNA extraction, dual rRNA depletion, library preparation, and sequencing with approximately 50 million short reads per sample to characterize rumen host-microbiome functional interactions. All deliverables are due by June 30, 2027, with performance commencing July 29, 2026. The Government will evaluate quotations using lowest price technically acceptable criteria. Quotations must remain firm for 90 calendar days and be submitted via email to amber.sampson@usda.gov with F.O.B. destination terms and electronic invoicing through the Invoice Processing Platform.