Maize DNA Extraction and Sequencing
Closed Solicitation Posted
This opportunity was awarded. See the award notice from .
- Solicitation number
- 1232SA26Q0568
- Agency
-
Agricultural Research Service
Department of Agriculture
- Responses due
- Set-aside
- Total Small Business
Opportunity facts
- NAICS code
- 541380 Testing Laboratories and Services
- PSC
- R499 Support- Professional: Other
- Place of performance
- Mississippi State, Mississippi 39762, United States
- Points of contact
Notice details come from SAM.gov. Updated .
About this opportunity
The Department of Agriculture Agricultural Research Service seeks DNA extraction and sequencing services for 384 maize leaf samples, with quotations due June 5, 2026, and questions due May 22, 2026. The procurement is a 100 percent small business set-aside under NAICS 541380 (Testing Laboratories and Services) with a size standard of $19 million.
Place of performance is Mississippi State University, Mississippi State, MS. The contractor must extract high-quality genomic DNA from freeze-dried maize leaf tissue with a minimum of 90 percent of samples meeting required DNA quality standards. Library preparation must use commercially available genotyping-by-sequencing methods employing the ApeKI restriction enzyme via PCR-free preparation. Sequencing shall use the Illumina NovaSeq X 10B or similar platform with 2 x 150 paired-end reads and a minimum sequencing depth of 25 per sample, with all samples run in a single batch. Deliverables must include genotype data with call rates of at least 95 percent per sample, aligned to the B73 RefGen_v5 reference genome assembly, yielding a minimum of 15,000 SNP markers distributed across all maize chromosomes. Data must be provided in both raw sequencing data and processed VCF format on hard disk; cloud-based delivery solutions are not acceptable. All processing must be completed within 15 weeks of sample receipt. Freeze-dried samples will be available for shipment approximately July 10, 2026. The period of performance runs from June 15 to September 30, 2026, with firm-fixed-price pricing on a job basis. Award will be made to the highest technically rated offeror whose price is fair and reasonable and whose past performance is acceptable or neutral. The RFQ number is 1232SA26Q0568. Questions and Answers documents dated May 15 and May 27 are attached.
Notice text
4 versions
Update #4 · Latest ·
***Questions and Answers in Attachments dated 27 May***
** Questions and Answers are in attachments - 15 May**
*Amended to include missing Statement of Work (SOW). SOW is in the attachments.*
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0568 is issued as a Request for Quotation (RFQ) for DNA Extraction and Sequencing services for 384 maize leaf samples.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services The small business size standard is $19,000,000 This acquisition is Choose an item. All responsible sources may submit a quotation which will be considered by the agency.
Update #3 ·
** Questions and Answers are in attachments - 15 May**
*Amended to include missing Statement of Work (SOW). SOW is in the attachments.*
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0568 is issued as a Request for Quotation (RFQ) for DNA Extraction and Sequencing services for 384 maize leaf samples.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services The small business size standard is $19,000,000 This acquisition is Choose an item. All responsible sources may submit a quotation which will be considered by the agency.
Update #2 ·
*Amended to include missing Statement of Work (SOW). SOW is in the attachments.*
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0568 is issued as a Request for Quotation (RFQ) for DNA Extraction and Sequencing services for 384 maize leaf samples.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services The small business size standard is $19,000,000 This acquisition is Choose an item. All responsible sources may submit a quotation which will be considered by the agency.
Update #1 ·
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0568 is issued as a Request for Quotation (RFQ) for DNA Extraction and Sequencing services for 384 maize leaf samples.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services The small business size standard is $19,000,000 This acquisition is Choose an item. All responsible sources may submit a quotation which will be considered by the agency.
Files
| File | Type | Posted |
|---|---|---|
| Questions and Answers 27 May.pdf | ||
| Questions and Answers 15 May.pdf | ||
| A04_SOW- Maize DNA_1232SA26Q0568.pdf | ||
| Sol_1232SA26Q0568.pdf |
Notice history
| Notice | Type | Posted |
|---|---|---|
| Maize DNA Extraction and Sequencing | Award Notice | |
| This notice | Solicitation |
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