DNA library prep & metabarcoding sequencing
Closed Solicitation Posted
- Solicitation number
- 140G0126Q0198
- Agency
- Office of Acquisitions and Grants US Geological Survey, Department of the Interior
- Responses due
- Set-aside
- Total Small Business
Opportunity facts
- NAICS code
- 541380 Testing Laboratories and Services
- Points of contact
-
- Susan Ruggles sruggles@usgs.gov (123) 456-7890
Notice details come from SAM.gov. Updated .
About this opportunity
The U.S. Geological Survey, Conservation Genetics Lab, Wetlands and Aquatic Research Center in Gainesville, Florida, seeks DNA library preparation and metabarcoding sequencing services with specialized bioinformatics on a firm fixed-price basis for one year, with quotes due July 2, 2026, and anticipated award July 16, 2026. The basis of award is lowest-price technically acceptable. While the SF 1449 Government File identifies this as a Women-Owned Small Business set-aside, the Federal Contract Opportunity Government Description and Amendment 0002 state the acquisition is not a small business set-aside; Amendment 0001 and Amendment 0002 represent the current governing language.
Place of performance is Gainesville, Florida. The contractor must process approximately 200–300 environmental DNA samples over the performance period, divided into batches of 20–50+ samples each, developing a unique reference DNA library incorporating USGS data and published sequences, optimizing a custom metabarcoding assay using six pooled single-target PCR assays per sample, and executing sequencing on Oxford Nanopore Promethion flow cells with 2-step PCR and super high accuracy base calling to achieve minimum 100,000 reads per sample. Assay target regions and PCR cycle conditions will not be disclosed until vendor selection due to unpublished government data. Key personnel must hold expertise in environmental DNA laboratory work and bioinformatics analysis, addressing eDNA-specific challenges including reference library limitations and contamination prevention. Deliverables include taxonomic assignments, exact sequence variants, fastq files, and quality control reports, all meeting publication-quality standards and delivered electronically within six weeks of sample intake. The contractor assumes full responsibility for sample receipt through final acceptance, including biohazardous waste disposal and maintenance of dedicated pre-PCR and post-PCR laboratory areas. NAICS code is 541380; PSC is B529. Vendors must be registered at SAM.gov. Quotes and inquiries must be emailed to sruggles@usgs.gov.
Notice text
3 versions
Update #3 · Latest ·
The purpose of this amendment is to provide the complete list of Questions and Answers. Refer to Form SF1449.
Update #2 ·
The purpose of this amendment is to provide Questions and Answers:
Question 1: Could the PI share the target region info or reference article so we can take a look? If default primers like 16S, ITS, or 18S are acceptable, that's fine; otherwise, we will need the PCR cycle conditions.
Answer 1: The assay is not published yet and so I can¿t share the details until the vendor is selected.
Question 2: The RFQ lists both CLIN 00010 and CLIN 00040 as ¿Metabarcoding Analyses¿ in accordance with Attachment 1. Can you clarify the intended distinction between CLIN 00010 and CLIN 00040, including whether they should be priced separately, whether they represent different assay/sample types, or whether one is an option/additional quantity?
Answer 2: CLIN 00010 and 00040 are for the same type of analyses, just for different funding accounts.
The U.S. Geological Survey, Conservation Genetics Lab, Wetlands and Aquatic Research Center in Gainesville, FL, requires genomic services of molecular library preparations and next generation sequencing with specialized bioinformatics as described in Attachment 1 statement of work.
The firm, fixed-price contract is for a one-year period.
Quotes shall provide pricing, and technical capabilities and experience to perform the work. The basis of award is lowest-price technically acceptable (see E.9. Evaluation). Quote submissions are due on or before 7/02/2026. The anticipated award date is 7/16/2026.
The acquisition is not a small business set-aside. The NAICS is 541380. Vendors must be registered at https://www.sam.gov/ to be awarded the contract.
All responsible sources may submit a quotation which shall be considered by the government. All correspondence and quotes MUST be emailed to sruggles@usgs.gov.
Update #1 ·
The U.S. Geological Survey, Conservation Genetics Lab, Wetlands and Aquatic Research Center in Gainesville, FL, requires genomic services of molecular library preparations and next generation sequencing with specialized bioinformatics as described in Attachment 1 statement of work.
The firm, fixed-price contract is for a one-year period.
Quotes shall provide pricing, and technical capabilities and experience to perform the work. The basis of award is lowest-price technically acceptable (see E.9. Evaluation). Quote submissions are due on or before 7/02/2026. The anticipated award date is 7/16/2026.
The acquisition is not a small business set-aside. The NAICS is 541380. Vendors must be registered at https://www.sam.gov/ to be awarded the contract.
All responsible sources may submit a quotation which shall be considered by the government. All correspondence and quotes MUST be emailed to sruggles@usgs.gov.
Attachments
| File | Type | Posted |
|---|---|---|
| Sol_140G0126Q0198_Amd_0002.pdf | ||
| Sol_140G0126Q0198_Amd_0001.pdf | ||
| Sol_140G0126Q0198.pdf | ||
| Attachment_1-_Performance_Work_Statement.docx | DOCX document |
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