Sol_140G0126Q0198_Amd_0002.pdf

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended, by one of the following methods:

(a) By completing items 8 and 15, and returning or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

E. IMPORTANT: is not is required copies to the issuing office.

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT 1. CONTRACT ID CODE

2. AMENDMENT/MODIFICATION NUMBER 3. EFFECTIVE DATE 4. REQUISITION/PURCHASE REQUISITION NUMBER 5. PROJECT NUMBER (If applicable)

7.ADMINISTERED BY (If other than Item 6) CODE

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

FACILITY CODE

9A. AMENDMENT OF SOLICITATION NUMBER

9B. DATED (SEE ITEM 11)

10A. MODIFICATION OF CONTRACT/ORDER NUMBER

10B. DATED (SEE ITEM 13)

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

The above numbered solicitation is amended as set forth in Item 14. is extended. is not extended.

12. ACCOUNTING AND APPROPRIATION DATA (If required) copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted;

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS.

IT MODIFIES THE CONTRACT/ORDER NUMBER AS DESCRIBED IN ITEM 14.

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER

NUMBER IN ITEM 10A.

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority)

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15C. DATE SIGNED

15A. NAME AND TITLE OF SIGNER (Type or print)

16C. DATE SIGNED

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

PAGE

6. ISSUED BY CODE

8. NAME AND ADDRESS OF CONTRACTOR (Number, street, county, State and ZIP Code) (X)

CODE

15B. CONTRACTOR/OFFEROR

(Signature of person authorized to sign)

16B. UNITED STATES OF AMERICA

(Signature of Contracting Officer)

Previous edition unusable

PAGESOF

Contractor

The hour and date specified for receipt of Offers to sign this document and return

Daniel Galvin

See continuation page

USGS OAG RESTON ACQUISITION BRANCH

205 NATIONAL CENTER

12201 SUNRISE VALLEY DRIVE

RESTON VA 20192

GRB

140G0126Q0198

06/25/2026

06/25/2026

1 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGES

NAME OF OFFEROR OR CONTRACTOR

SUPPLIES/SERVICES

(B)

UNIT

(D)

UNIT PRICE

(E)

AMOUNT

(F)

OPTIONAL FORM 336 (4-86)

Sponsored by GSA FAR (48 CFR) 53.110

ITEM NO.

(A)

QUANTITY

(C)

NSN 7540-01-152-8067

140G0126Q0198/0002

DNA library preparation and metabarcoding sequencing services. This contract supplies genetic sequencing services

Questions & Answers:

Restate Question 1: Could the PI share the target region info or reference article so we can take a look? If default primers like 16S, ITS, or 18S are acceptable, that's fine;

otherwise, we will need the PCR cycle conditions.

Answer 1: The assay is not published yet and so

I cannot share the details until the vendor is selected.

Restate Question 2: The RFQ lists both CLIN

00010 and CLIN 00040 as Metabarcoding Analyses in accordance with Attachment 1. Can you clarify the intended distinction between CLIN

00010 and CLIN 00040, including whether they should be priced separately, whether they represent different assay/sample types, or whether one is an option/additional quantity?

Answer 2: CLIN 00010 and 00040 are for the same type of analyses, just for different funding accounts.

Question 3: Which specific gene regions do you want to target (e.g., full-length 16S for bacteria, ITS for fungi, etc.)? What are the exact custom primer sequences that you want to use for each target? What are the specific PCR protocols (cycling conditions, annealing temperatures, master mix preferences) required for each target?

Answer 3: This is unpublished government data that cannot be shared until BAO approved. The information and will be shared once the vender is selected.

Question 4: Could you clarify if you are running 6 separate single-target PCR assays per sample and pooling them (which is recommended), or attempting a 6-target multiplex PCR in a single tube?

Answer 4: Yes, 6 separate single-target PCR assays per sample and pooling.

Continued...

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGES

NAME OF OFFEROR OR CONTRACTOR

SUPPLIES/SERVICES

(B)

UNIT

(D)

UNIT PRICE

(E)

AMOUNT

(F)

OPTIONAL FORM 336 (4-86)

Sponsored by GSA FAR (48 CFR) 53.110

ITEM NO.

(A)

QUANTITY

(C)

NSN 7540-01-152-8067

140G0126Q0198/0002

Question 5: What is the total number of separate batches you intend to submit, how many individual samples will be included within each specific batch, and when will each batch be sent approximately?

Answer 5: We estimate a total of 300 samples and approximate batch size from 20-50+ per batch.

The U.S. Geological Survey, Conservation

Genetics Lab, Wetlands and Aquatic Research

Center in Gainesville, FL, requires genomic services of molecular library preparations and next generation sequencing with specialized bioinformatics as described in Attachment 1 statement of work.

The firm, fixed-price contract is for a one-year period.

Quotes shall provide pricing, and technical capabilities and experience to perform the work. The basis of award is lowest-price technically acceptable (see E.9. Evaluation).

Quote submissions are due on or before

7/02/2026, 1100 ED. The anticipated award date is 7/16/2026.

The acquisition is not a small business set-aside. The NAICS is 541380. Vendors must be registered at https://www.sam.gov/ to be awarded the contract.

All responsible sources may submit a quotation which shall be considered by the government.

All correspondence and quotes MUST be emailed to sruggles@usgs.gov.

For questions, contact Susan Ruggles, sruggles@usgs.gov

Sponsored by GSA FAR (48 CFR) 53.110