Amendment # 1 - Response to questions - Acquisition of Sequencing Services

The FDA's Office of Acquisitions and Grant Services is procuring whole genome sequencing services through solicitation 75F40125Q00266, specifically seeking PacBio long read whole genome sequencing on 16 pig cell samples. The contractor must generate approximately 30x whole genome sequence coverage, including DNA extraction, library preparation using PacBio Revio platform, and delivery of raw sequencing reads. Technical specifications require libraries capable of 15-20kb fragment lengths, achieving a mean read length of 10,000 bp with Q25 quality, and producing approximately 85 Gb raw throughput per sample. The government will use a lowest price technically acceptable (LPTA) evaluation method to select the winning contractor. Amendment #1 clarified that samples will be provided as frozen porcine tissue or frozen cell pellets, and quotes are due by 3:00 p.m. Eastern Time on August 7, 2025.

This opportunity is designated as a 100% small business set-aside under NAICS code 541715, with no incumbent contractors identified in the documentation. The contract structure is firm-fixed-price with a performance period from September 19, 2025 to September 18, 2026, though the total estimated value is not explicitly disclosed in the available documents. The FDA has specified a desired 13-week turnaround time from final sample receipt, with flexibility for minor timing adjustments across individual workflow steps as long as the total project completion does not exceed 13 weeks. Performance will take place in Silver Spring, Maryland, and contractors may submit proposals while their NAICS code updates are pending, with screenshot confirmations acceptable for evaluation purposes.