Statement of Work DRAFT.pdf
PDF 157 KB Posted
- Attached to
- Metabarcoding Forest Insect Gut Contents
- Solicitation number
- 1157030
- Issued by
-
Department of Agriculture Forest Service
About this file
This is a Statement of Work (SOW) issued by the US Department of Agriculture dated August 7, 2025, for a project titled "Metabarcoding forest insect gut contents." The scope of work involves targeted amplicon sequencing of DNA extracted from the guts of native bee species and the Asian needle ant to understand the resource requirements of native pollinators and ecological impacts of invasive species.
The contractor's technical requirements include five key tasks: adding Illumina amplicons to DNA extracts, cleaning up PCR product, conducting library pooling, performing pre-sequencing quality control, and proceeding with Illumina NextSeq2000 2x300bp flow cell (P1) sequencing. The government will furnish DNA extracts and will analyze the resulting sequencing data. Dr. Walter Lorenz from the Georgia Genomics and Bioinformatics Core will serve as the key personnel overseeing the sequencing work. The deliverable schedule requires completion within six months of receiving the DNA extracts, with no travel required. The resulting data will be used by Forest Service researchers for analysis and subsequent publication in peer-reviewed journals for public dissemination.
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US Department of Agriculture (USDA)
Statement of Work
August 07 2025
Contents Project General Information
1.0 Scope of Work
2.0 Background
Contractor Requirements
3.0 Technical Requirements / Tasks
4.0 Government Furnished
5.0 Deliverables / Schedule
6.0 Travel
7.0 Contractor’s Key Personnel
8.0 Security Requirements
9.0 Data Rights
10.0 Section 508 – Electronic and Information Technology Standards Attachment
Project
Metabarcoding forest insect gut contents
General Information
1.0 Scope of Work
Targeted amplicon sequencing of DNA extracted from the guts of native bee species and the Asian needle ant.
2.0 Background
To better understand the resource requirements of native pollinators (i.e., which tree species represent important sources of pollen in forests) as well as the ecological impacts of the invasive Asian needle ant, amplicon sequencing is needed to determine what these insects have been ingesting.
Contractor Requirements
3.0 Technical Requirements / Tasks
Beginning with DNA extracts, the contractor will 1) add Illumina amplicons, 2) clean up PCR product, 3) conduct library pooling, 4) perform pre-sequencing QC, 5) proceed with Illumina NextSeq2000 2x300bp flow cell (P1)
4.0 Government Furnished
FS researchers will provide contractor with DNA extracts and will analyze the sequencing data that are produced.
5.0 Deliverables / Schedule
Sequencing will be completed within six months of receiving the DNA extracts.
6.0 Travel
None
7.0 Contractor’s Key Personnel
Dr. Walter Lorenz (Assistant Research Scientist, Georgia Genomics and Bioinformatics Core Lead Consultant) will oversee the sequencing. With years of experience and expertise in Illumina sequencing, he is perfectly suited for this role.
His email and phone number are: wlorenz@uga.edu, 706-542-7974
8.0 Security Requirements
None
9.0 Data Rights
Data will be used by FS researchers to complete their analysis which will then be shared with the general public in the form of peer-reviewed publications
10.0 Section 508 – Electronic and Information Technology Standards
NA
Attachment
NA
mailto:wlorenz@uga.edu
| Project |
| General Information |
| 1.0 Scope of Work |
| 2.0 Background |
| Contractor Requirements |
| 3.0 Technical Requirements / Tasks |
| 4.0 Government Furnished |
| 5.0 Deliverables / Schedule |
| 6.0 Travel |
| 7.0 Contractor’s Key Personnel |
| 8.0 Security Requirements |
| 9.0 Data Rights |
| 10.0 Section 508 – Electronic and Information Technology Standards |
| Attachment |
File details come from the government source that posted it. Updated .