Statement of Work.pdf

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DNA Analyses
Solicitation number
N6660426Q0455
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This is a Statement of Work (SOW)/Performance Work Statement (PWS) for microbial analysis services supporting the Naval Undersea Warfare Center Division Newport (NUWCDIVNPT) and the New Attack Submarine Program Office (PMS-351).

The contractor shall perform 16S and 18S DNA/rRNA analyses on biofilm samples collected from test panels deployed at various ocean depths (nominally 50, 250, 500, and 950 meters) to identify microbial types and population percentages. The work supports NUWCDIVNPT's research on deep sea biofouling and the effects of hydrostatic pressure on antifouling paints and coatings. All analyses will be conducted at the contractor's laboratory using samples provided by NUWCDIVNPT and/or universities under contract with the Navy. The contractor must retain samples for 30 days after report issuance to allow for re-testing if required by the Navy, then dispose of samples according to applicable Federal, State, and local regulations. The primary deliverables are: (1) reports documenting 16S and 18S analysis findings for each sample, including taxonomic assignments for all reads from available reference databases (CDRL A001); and (2) raw data from all analyses in Excel format (CDRL A002). The work is funded through RDT&E dollars and authorized for Code 1512 users. Section 3.0 references Standard 16S and 18S sample preparation and analysis protocols as the applicable document. Security requirements include adherence to 32 CFR Part 117 (NISPOM), protection of Controlled Unclassified Information (CUI) and Covered Defense Information per DoD Instruction 5200.48, implementation of Operations Security (OPSEC) programs, and prompt reporting of security incidents and CUI disclosures to the Contracting Officer's Representative, Technical Point of Contact, and the Cognizant Security Office.

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Other files

File Type Posted
RFQ N6660426Q0455 Rev02.pdf PDF
RFQ N6660426Q0455 Amendment.pdf PDF
Question and Answers.pdf PDF
CDRL.pdf PDF
RFQ N6660426Q0455.pdf PDF

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v4.10_DECEMBER2025

STATEMENT OF WORK (SOW)/PERFORMANCE WORK STATEMENT (PWS)

1.0 Background

The Naval Undersea Warfare Center Division, Newport (NUWCDIVNPT) has several projects associated with documenting the types and extent of biofouling that occurs in the deep sea. Very little is known about deep sea biofouling at the present time. One of the items of great interest to the program is a determination of the kinds of microbes that are present in the biofilms that are expected to be found on test panels secured at all depths (nominally 50 meters, 250 meters, 500 meters and 950 meters). NUWCDIVNPT is also testing the effect of hydrostatic pressure on various antifouling paints and coatings by subjecting such materials to hydrostatic pressure cycling and hydrostatic pressure dwells and then exposing the panels in shallow water test sites to see if any differences in antifouling performance occur. Identification of microbes in the biofilms on the sample plates will be a very important part of the project. NUWCDIVNPT does not own the DNA analysis equipment needed to make these identifications; thus, samples will be collected from test panels and sent to a third party testing laboratory with the appropriate equipment and expertise to perform the analyses and report the results back to NUWCDIVNPT.

1.1 Places of Performance

All analyses will be performed at the contractor’s laboratory using samples sent to that location from NUWC Division Newport and/or universities under contract with

NUWCDIVNPT.

1.2 Authorized Users

Code 1512

1.3 Sponsors

New Attack Submarine Program Office (PMS-351)

1.4 Types of Funding

RDT&E

2.0 Scope

The contractor shall perform 16S and 18S analyses of biofilm samples to identify the type and population percentages of the microbes comprising the samples. The contractor shall provide results and raw data from all analyses performed.

2.1 Program or Systems Supported

New Attack Submarine Program (PMS-351)

3.0 Applicable Documents

The contractor shall perform the tasking required in Section 4.0 in accordance with (IAW) the below Applicable Documents (ADs).

NUMBER TITLE REVISION/

DATE

APPLICABLE

SOW TASK

3.1 Standard 16S and 18S sample preparation

and analysis protocols.

Current ALL

4.0 Technical Requirements

The contractor shall perform the following tasks in accordance with (IAW) Applicable Documents in Section 3.0 and using GFI in Section 6.0.

4.1 The contractor shall analyze water samples provided by NUWCDIVNPT and/or universities working with NUWCDIVNPT (samples as marked) using standard 16S (bacteria/archaea) and 18S (eukaryotes/microbial fungi) DNA/rRNA analyses protocols. The goal of the testing is to provide a survey of the diversity of microbial lifeforms within the samples, including taxonomic assignments for all reads using available reference databases.

4.1.1 All samples will have identification markings applied by the Navy or universities working for the Navy. Those markings should be used to identify samples and the data associated with those samples.

4.1.2 The contractor shall retain samples for 30 days after issuance of report in case they need to be re-run for technical reasons. The Navy will advise the contractor if any tests on any samples need to be re-run.

4.1.3 Once all testing (and any required re-testing) has been completed, the contractor may dispose of the samples in accordance with applicable Federal, State and local laws and requirements.

4.1.4 The contractor shall submit reports documenting findings from the 16S and 18S analyses conducted on each sample, including:

a. Taxonomic assignments for all reads from available databases.

b. Raw data from 16S and 18S analyses for each sample. (in the form of an excel file).

Deliverables: CDRLs A001 and A002

5.0 Progress Reports

Reserved

4 Government Furnished Information

The following Government Furnished Information (GFI) will be made available under this contract.

Number Title Applicable SOW Task Number

6.1 Sample labels/ID numbers (these will be

annotated on samples sent to the contractor) ALL

7.0 Government Furnished Property

8.0 Quality Surveillance and Performance Standards

9.0 Information and Communication Technology (ICT) Accessibility Requirements

The Government has determined that this procurement is not Information and Communication Technology (ICT).

10.0 Security Compliance

10.1 Program Protection

SECURITY: All Contractor personnel shall adhere to the Security provisions of 32 CFR Part 117

– National Industrial Security Program Operating Manual (NISPOM). While performing work at a Government Facility, Contractor personnel shall comply with the security regulations of the host facility. Applicable FAR, DFARS, NMCARS clauses, and NAVSEA text shall be adhered to in the performance of this contract. Security incidents shall be promptly reported through the companies Facility Security Officer (FSO), to the Contracting Officer’s Representative (COR), Technical Point of Contact (TPOC), and the Cognizant Security Office to NUWCDIVNPT Security.

Controlled Unclassified Information (CUI) including Legacy FOUO and Covered Defense Information (meeting the definition of 48 CFR 252.204–7012(a)) generated and/or provided under this contract shall be marked and safeguarded as specified in DoD Instruction 5200.48, CUI available at:

https://www.esd.whs.mil/Portals/54/Documents/DD/issuances/dodi/520048p.PDF. Any product containing Covered Defense Information shall be assigned a distribution statement (distribution statements B through F) in accordance with DoDI 5230.24 (Distribution Statements on Technical Documents); and DoDI 5230.24, Enclosure 3 Procedures, available at https://www.esd.whs.mil/Portals/54/Documents/DD/issuances/dodi/523024p.pdf

INFORMATION SECURITY: If the work is performed at the Contractor's facility, the Contractor shall implement and maintain security procedures and controls to prevent unauthorized disclosure of classified information and controlled unclassified information (CUI) and to control distribution of CUI in accordance with National Industrial Security Program Operating Manual (NISPOM) codifying 32 CFR Part 117, NISPOM Rule, and SECNAV M- 5510.36B. If the work is performed at the Government's facility, the Contractor shall comply with facility policy.

CUI INCIDENT REPORTING AND RESPONSE: The Contractor shall promptly report any unauthorized, inadvertent, or illegal release or disclosure of CUI to the Contracting Officer’s Representative / Technical Point of Contact (TPOC), Procuring Contracting Officer, and the Security Office. Contractor personnel shall coordinate this effort through the relevant industry site FSO.

PUBLIC RELEASE: Any controlled unclassified information pertaining to this contract shall not be released for public dissemination, including posting to any social media sites such as Facebook or Twitter, unless it has been approved for public release by appropriate U.S.

Government authority. Proposed public releases shall be submitted for approval prior to release through the appropriate U.S. Government Office.

10.2 Operations Security (OPSEC)

OPSEC is a process that identifies critical information to determine if friendly actions can be observed by adversary intelligence systems, determines if information obtained by adversaries could be interpreted to be useful to them, and then executes selected measures that eliminate or reduce adversary exploitation of friendly critical information.

The Contractor shall develop and implement, and update and maintain an OPSEC program to protect controlled unclassified and classified activities, information, equipment, and material used or developed by the Contractor and any subcontractor during performance of the contract.

The Contractor shall be responsible for the subcontractor implementation of the OPSEC requirements. The Contractor developed OPSEC program may include Information Assurance and Communications Security (COMSEC). The OPSEC program shall be in accordance with National Security Presidential Memorandum (NSPM) 28, and at a minimum shall include:

1) Assignment of responsibility for OPSEC direction and implementation.

2) Issuance of procedures and planning guidance for the use of OPSEC techniques to identify vulnerabilities and apply applicable countermeasures.

3) Establishment of OPSEC education and awareness training. To include initial and annual OPSEC training.

4) Provisions for management, annual review, and evaluation of OPSEC programs.

5) Flow down of OPSEC requirements to subcontractors when applicable.

While performing aboard Government sites, the contractor shall: comply with all OPSEC instructions and policies; include OPSEC as part of its ongoing security awareness program and take all required Agency training; Be responsive to the Supporting OPSEC Manager on a non-interference basis; and Protect sensitive unclassified information and activities, which could compromise classified information or operations, or degrade the planning and execution of operations performed by the Requiring Organization and contractor in support of the mission.

10.3 Electronic Spillage

Electronic Spillage (ES) is defined as a situation where information of higher classification than a system is authorized to process is introduced into that system, intentionally or otherwise. If a Contractor is determined to be responsible for an ES, all direct and indirect costs incurred by the Government for ES remediation will be charged to the Contractor.

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