Requirements.docx
DOCX document 18 KB Posted
- Attached to
- Bio-Rad QX600 Droplet Digital PCR System
- Solicitation number
- 75N98026Q00916
About this file
This is a Requirements document for a Firm Fixed Price Purchase Order solicitation (75N98026Q00916) issued by the Department of Health and Human Services National Institutes of Health Office of Logistics and Acquisition Operations. The government requires procurement of a digital droplet PCR (ddPCR) platform for delivery to the National Institutes of Health, specifically one Bio-Rad QX600 Droplet Digital PCR System and one Bio-Rad PTC Harmony Deepwell Thermal Cycler.
The instrument must perform digital droplet PCR using partitioned droplet technology with the capability to partition each 20 μL reaction into approximately 20,000 droplets, support six-color fluorescence detection, process 96-well plates, and provide absolute nucleic acid quantification without reliance on standard curves. The system must support multiple applications including mutation detection, copy number variation analysis, gene expression analysis, and viral titer analysis. Required deliverables include integrated software capable of automated thresholding, Poisson analysis, multiplex analysis, and results export, along with all necessary computer hardware and system software. Training is explicitly not included as part of this purchase order, and the contractor has no obligation to provide user training.
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Other files
| File | Type | Posted |
|---|---|---|
| Presolicitation 75N98026Q00916.docx | DOCX document |
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Text version
Requirements The laboratory requires a digital droplet PCR (ddPCR) platform capable of absolute nucleic acid quantification with high sensitivity and multiplex capability.
The quoted system provides up to six-color multiplex detection, processes up to 96 samples per run, and performs absolute nucleic acid quantification
Type of Order:
Firm Fixed Price Purchase Order Special Order Requirements:
The instrument shall:
· Perform digital droplet PCR using partitioned droplet technology.
· Partition each 20 μL reaction into approximately 20,000 droplets.
· Support six-color fluorescence detection.
· Process 96-well plates.
· Provide absolute nucleic acid quantification without reliance on standard curves.
· Support applications including:
· mutation detection
· copy number variation
· gene expression
· viral titer analysis
· Include software capable of automated thresholding, Poisson analysis, multiplex analysis, and export of results.
· Include all required computer hardware and system software.
· Include a compatible thermal cycler capable of operating 96-well deepwell PCR plates.
These specifications are consistent with the manufacturer's published technical specifications.
The contractor shall deliver the following equipment to the National Institutes of Health (NIH):
· One (1) Bio-Rad QX600 Droplet Digital PCR System
· One (1) Bio-Rad PTC Harmony Deepwell Thermal Cycler
Training:
Training is not included under this purchase. The contractor is not required to provide user training as part of this award.
File details come from the government source that posted it. Updated .