Requirement 7.docx
DOCX document 17 KB Posted
- Attached to
- Olink Explorer >5400
- Solicitation number
- 75N98026Q00800
About this file
This is a requirement specification document for a federal solicitation (75N98026Q00800) issued by the Department of Health and Human Services National Institutes of Health Office of Logistics and Acquisition Operations. The requirement specifies the Olink Explorer >5400 proteomics platform for use in laboratory research studying inflammation, innate resistance, and commensal microbiota in relation to carcinogenesis, cancer progression, and cancer therapy.
The specified product must measure approximately 5,400+ human proteins simultaneously using Proximity Extension Assay (PEA) technology with dual antibody recognition coupled to DNA barcoding and next-generation sequencing readout. The platform requires minimal sample input of only 2 microliters of plasma, serum, or other sample types, with reported 99.5% specificity and negligible cross-reactivity due to the dual-recognition antibody design. The technology offers an approximate 10 orders of magnitude dynamic range (fg/mL to mg/mL) for detecting both low- and high-abundance proteins. Key attributes include broad proteome coverage with antibody-based specificity, excellent sensitivity for low-abundance circulating proteins such as cytokines and signaling proteins, extremely low sample-volume requirements, and a highly standardized platform suitable for epidemiology and multi-omics research applications.
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Requirements:
2.0 BACKGROUND
Our lab studies the role of inflammation/innate resistance and commensal microbiota in carcinogenesis, cancer progression and therapy of cancer.
The following product features/characteristics are required for this requirement:
Protein Coverage - Measures ~5,400+ human proteins simultaneously in a single workflow.
Technology - Uses Proximity Extension Assay (PEA) with dual antibody recognition coupled to DNA barcoding and next-generation sequencing (NGS) readout.
Sample input – requires only 2 ul of plasma, serum, and other sample types
Specificity - Reported 99.5% specificity with negligible cross-reactivity due to dual-recognition antibody design.
Dynamic Range - Approximately 10 orders of magnitude (fg/mL to mg/mL), enabling detection of both low- and high-abundance proteins.
Key strengths
· Very broad proteome coverage while maintaining antibody-based specificity.
· Excellent sensitivity for low-abundance circulating proteins (especially cytokines and signaling proteins).
· Extremely low sample-volume requirement.
· Highly standardized platform, making it attractive for epidemiology and multi-omics studies.
File details come from the government source that posted it. Updated .