PD PCA-NCATS-02349.pdf
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- Attached to
- Patch-Clamp Amplifier System Federal contract opportunity
- Solicitation number
- 75N98026Q00195
About this file
This is a Purchase Description document prepared under FAR Part 11 for the procurement of a dual-channel patch-clamp amplifier system.
The National Center for Advancing Translational Science (NCATS) Stem Cell Translation Laboratory (SCTL) requires one dual-channel patch-clamp amplifier system to support electrophysiological characterization of human pluripotent stem cell-derived cardiomyocytes, neurons, and other excitable cell types for Project #TR000244-10, which commenced July 1, 2024. The system must provide high-resolution measurement of ionic currents and membrane potentials to evaluate cellular maturity, subtype identity, and functional phenotype. The equipment is required as soon as possible to avoid delays in ongoing experimental timelines.
The minimum requirements specify two fully independent patch-clamp amplifiers within a single mainframe capable of simultaneous, independently configurable voltage-clamp and current-clamp operation. Key technical specifications include capacitive feedback headstages optimized for low-noise recordings, femtoampere-range input bias current, integrated pipette and whole-cell capacitance compensation, series resistance compensation with ≥80% compensation capability in both prediction and correction modes, bridge balance capability, ≥100 kHz bandwidth in voltage-clamp mode, low RMS noise suitable for single-channel recordings, integrated control software with independent channel configuration, calibration model cells for verification, and mounting hardware compatible with standard micromanipulator systems. The system must be fully compatible with existing laboratory electrophysiology workflows and data acquisition infrastructure. No options, special instructions, or alternative delivery arrangements are specified.
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| File | Type | Posted |
|---|---|---|
| Combined Synopsis Solicitation - Competitive 75N98026Q00195.pdf |
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Version 11-30-2016-1 FAR Part 11—Describing Agency Needs
PURCHASE DESCRIPTION
1. Statement of Need and Purpose:
This equipment is required to support functional and electrophysiological characterization of human pluripotent stem cell (hPSC)-differentiated cell types within the Stem Cell Translation Laboratory (SCTL) and the National Center for Advancing Translational Science (NCATS). The amplifier system will enable high-resolution measurement of ionic currents and membrane potentials necessary to evaluate cellular maturity, subtype identity, and functional phenotype in electrically excitable cells.
2. Background Information and Project details:
Electrophysiological characterization is a critical component of validating hPSC-derived cardiomyocytes, neurons, and other excitable cell types. Accurate measurement of voltage- and current-dependent cellular responses is required to assess ion channel function, action potential properties, synaptic activity, and pharmacologic responses.
Project #TR000244-10 (project start date: 07/01/2024) includes studies that require low-noise, high-stability patch-clamp recordings in both voltage-clamp and current-clamp configurations. The requested system will provide the precision, sensitivity, and experimental flexibility necessary to generate reproducible, publication-quality electrophysiological data.
3. Generic Name of Product:
Patch-Clamp Amplifier System
4. Purchase Description and Salient Characteristics:
The Government requires a dual-channel patch-clamp amplifier system designed for high-precision electrophysiological recordings from single cells. The system must meet the following minimum requirements:
• Two fully independent patch-clamp amplifiers housed within a single mainframe
• Simultaneous, independently configurable voltage-clamp and current-clamp operation
• Capacitive feedback headstages optimized for low-noise single-channel and whole-cell recordings
• Input bias current in the femtoampere range
• Integrated pipette capacitance neutralization and whole-cell capacitance compensation
• Series resistance compensation with both prediction and correction modes capable of ≥80% compensation
• Bridge balance capability for current-clamp recordings
• High bandwidth performance (≥100 kHz in voltage-clamp mode)
• Low RMS noise suitable for single-channel recordings
• Integrated control software that allows independent configuration of each channel and compatibility with existing laboratory data acquisition hardware
• Calibration model cells for system verification
• Mounting hardware and electrode holders compatible with standard micromanipulator systems
The system must be fully compatible with existing electrophysiology workflows and data acquisition infrastructure currently in use within the laboratory.
5. Quantity:
One (1) system
6. Delivery Date or Period of Performance: Delivery requested as soon as possible to avoid delays in ongoing
Version 11-30-2016-1 FAR Part 11—Describing Agency Needs experimental timelines.
7. Option(s): N/A
8. Special Instructions: N/A
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