DRAFT Attachment 3 - Limit of Detection REQUIREMENT 26-374.pdf
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- Attached to
- Microcystin Toxin Analysis Federal contract opportunity
- Solicitation number
- REQUIREMENTS-26-3745
About this file
This document is a technical specification table for microcystin toxin analysis prepared by NOAA's Great Lakes Environmental Research Laboratory (GLERL). The table presents analytical data for the detection and quantification of 20 microcystin and related cyanobacterial toxin analytes using specified methods. Each analyte entry includes the method detection limit measured in parts per trillion (ppt), upper and lower Percent Ion Ratios (PIR), R² values indicating standard curve quality, and the linearity range in ppt.
The detection limits for the analytes range from 0.25 ppt (Aeruginosamide B) to 3.96 ppt (MC-LR), with R² values consistently exceeding 0.997, demonstrating high analytical precision and reliability. Standard curve linearity ranges vary from 0.5-500 ppt to 5-500 ppt depending on the specific analyte. The analytes include various microcystin variants (MC-RR, MC-YR, MC-HtyR, MC-LR, MC-HilR, MC-WR, MC-LA, MC-LY, MC-LW, MC-LF), anabaenopeptins, oscillaginin A, microginin, oscillamide Y, and aeruginosamides. This technical data appears to establish performance standards for toxin analysis capabilities relevant to Great Lakes water quality monitoring and environmental research purposes.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| DRAFT Attachment 1 - Performance Work Statement REQUIREMENTS-26-3745.pdf | ||
| DRAFT Attachment 2 - Technical Specifications REQUIREMENT 26-3745.pdf |
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Text version
DRAFT
NOAA Great Lakes Environmental Research Laboratory (GLERL) Microcystin Toxin Analysis
Limit of Detection
Analyte Method
Detection Limit (ppt)
Upper
PIR*
Lower PIR* R2 value Standard Curve Linearity range (ppt)
D-Asp3Dhb7 MC-RR 1.61 117.32 82.84 0.9989 5-500
MC-RR 0.67 114.09 99.74 0.9990 5-500
Nodularin 1.64 149.18 79.11 0.9970 5-500
MC-YR 1.71 133.74 60.70 0.9976 1-500
MC-HtyR 2.95 141.35 78.30 0.9983 5-500
MC-LR 3.96 146.59 61.98 0.9978 5-500
D-Asp3 MC-LR 2.04 129.21 85.61 0.9972 1-500 MC-HilR 1.24 146.63 97.77 0.9978 5-500
MC-WR 3.91 140.81 57.18 0.9986 5-500
MC-LA 2.23 137.07 89.45 0.9983 1-500
MC-LY 1.90 146.18 64.83 0.9990 1-500
MC-LW 1.45 120.92 58.95 0.9988 1-500
MC-LF 2.18 147.23 53.96 0.9990 1-500
Anabaenopeptin A 3.12 146.60 79.97 0.999 5-500 Anabaenopeptin B 0.36 111.50 96.13 0.999 1-500 Oscillaginin A 1.14 132.69 83.92 0.999 1-500 Microginin 690 methyl ester
2.41 124.01 72.40 1.000 1-500
Oscillamide Y 1.36 147.44 89.47 0.999 1-500 Aeruginosamide B 0.25 133.03 78.82 0.997 0.5-500 Aeruginosamide C 0.33 133.11 62.18 1.000 0.5-500
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