Competitive COMBINED SYNOPSIS (006).pdf

COMBINED SYNOPSIS / SOLICITATION (COMPETITIVE)

Title: Whole genome sequencing for investigation of age-related chronic disease in HANDLS participants

(i) This is a combined synopsis/solicitation for commercial items prepared in accordance with FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested, and a separate written solicitation will not be issued.

(ii) The solicitation number is 75N98026R00166. This solicitation is issued as a competitive combined synopsis/solicitation for commercial products or commercial services. The Government intends to award a contract or purchase order resulting from this solicitation using full and open competition.

This acquisition is conducted under the authority of FAR 12.603, Streamlined Solicitation for Commercial Products or Commercial Services.

The National Institutes of Health (NIH), Office of the Director, Office of Acquisitions, seeks to award a Fixed Price Purchase Order to the responsible offeror whose proposal represents the best value to the Government.

(iii) The solicitation document and incorporated provisions and clauses are those in effect through current Federal Acquisition Regulations (RFO).

(iv) The associated NAICS code is 541714 — Research and Development in Biotechnology (except Nanobiotechnology), and the small business size standard is 1,000 employees.

This requirement is unrestricted.

(v) Description of Requirement:

Investigators in the Healthy Aging in Neighborhoods of Diversity across the Life Span (HANDLS) study require whole genome sequencing (WGS) processing to expand our current research examining whether genetic variants contribute to age-related chronic diseases. Samples from HANDLS participants have been collected over 20 years and many are over 10 years old. A contract is required because the Government does not have available facilities or resources to perform this work consistent with other WGS performed on other HANDLS samples.

The objective of this work is expanding our current research to examine health differences associated with age-related chronic diseases through whole genome sequencing to investigate the contributions of genomics to the burden of disease incidence, prevalence, morbidity, and mortality in populations within the United States. The long-term goal is to create a consistent dataset of whole genome sequencing to complement the longitudinal HANDLS behavior and health data.

The contractor will deliver plates to the Government sufficient to run 600 samples, some of which have been stored for ten or more years. The contractor will perform whole genome sequencing, and specifically shall:

• Sequence libraries on the NovaSeq X-Plus platform with 25B-300 flow cells using 150 bp paired end runs and NovaSeq workflow-series 10B sequencing run

• confirm genomic DNA quantity and quality by performing DNA concentration measurements and volume checks

• Re-attempt sequencing in the case that DNA quality and quantity is sufficient, but sample fails sequencing-based quality control

• Examine agarose gels on all samples as an industry-standard method for screening DNA marker quality prepare PCR-free sequence libraries for all samples

• Sequence the libraries at a minimum of 30x coverage with 2×150 base pair runs

• Perform primary and secondary analysis of the sequencing data including but not limited to read alignment, variant calling, and quality control analyses

• Quality control checks will include confirming sex, identifying unexpected duplicates and relatedness, confirming relatedness, providing sample performance information and sample identity confirmation against the sequencing data, in addition to confirming coverage, contamination, mapped reads, and other general quality metrics.

• Alignment and variant calling will be performed with the DRAGEN Germline pipeline. After quality is confirmed, joint variant calling should be performed producing a multi-sample VCF file.

• Provide written summaries and documentation of the methods used to perform the whole genome sequencing pipeline

• Supply the whole genome sequencing data in a mutually acceptable electronic format that preserves study participants’ confidentiality. Data delivered will include:

o per sample CRAMs o per sample gVCFs o per sample VCFs o joint-called multi-sample VCF o per sample structural variant and CNV calls o QC report and data dictionary o Sample manifest with flagged problems

Following data delivery and upon request by the Government, the contractor shall provide attendance at Government laboratory staff meetings, and the contractor shall provide technical consultations as needed.

The contractor will also ship overnight on dry ice any leftover samples on plates to the Biomedical Research Center in Baltimore, MD.

(vi) Delivery Location:

Baltimore, MD Exact address will be provided at time of award notification.

(vii) [Reserved, if needed]

(viii) FAR 52.204-7, System for Award Management, applies to this acquisition.

(ix) The provision at FAR 52.212-1, Instructions to Offerors—Commercial Items, applies to this acquisition. Add any agency-specific instructions as required.

(x) The provision at FAR 52.212-2, Evaluation—Commercial Items, applies to this acquisition.

(a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation represents the best value to the Government, price and other factors considered.

(b) Evaluation Factors: (Customize as needed)

Please see the attached evaluation criteria attached to this SAM posting, these criteria present the evaluation factors and the relative importance of each.

(c) A written notice of award or acceptance of an offer, issued within the time specified in the offer, shall result in a binding contract without further action by either party. The Government reserves the right to accept any offer before the expiration date.

(xi) FAR 52.212-4, Contract Terms and Conditions—Commercial Items, applies to this acquisition.

(xii) Additional contract requirements or terms and conditions, as determined necessary by the Contracting Officer and consistent with customary commercial practices:

“None.”

(xiii) The Defense Priorities and Allocations System (DPAS) is not applicable.

(xiv) Submission Requirements:

Responses must include sufficient information to demonstrate the offeror’s capability to provide the required product or service. Quotes shall include, at a minimum:

• Unit and total price

• Shipping and handling costs

• Delivery timeframe after award

• Delivery terms

• F.O.B. point (destination or origin)

• Product or catalog numbers (if applicable)

• Product/service description

• Not exceed 10 pages (addendums and supporting data are not included in the page limitation).

Additional evaluation considerations may include past performance, warranty, lifecycle cost, maintenance availability, and environmental or energy efficiency.

Responses must include the Unique Entity Identifier (UEI), Taxpayer Identification Number (TIN), and business size certification. All offerors must be actively registered in the System for Award Management (SAM) at www.sam.gov.

Deadline for questions is Friday September 18, 2026, at 9:00 AM EDT.

All responses must be received by September 24, 2026, at 9:00 AM EDT and must reference solicitation number 75N98026R00166. Responses may be submitted electronically to brian.lind@nih.gov.

Due to the urgency and getting an award processed by September 30, 2026, there will be no extensions to any of the deadline and response dates.

(xv) Point of Contact:

Brian Lind Brian.lind@nih.gov http://www.sam.gov/ mailto:brian.lind@nih.gov