Attachment D_FINAL.pdf
PDF 1 MB Posted
- Solicitation number
- ITB-14743
- Issued by
- Leon County, Florida
About this file
This is a sampling protocol document developed by the University of Florida's Aquatic and Wetland Plant Science Lab that provides detailed instructions for collecting and preparing eelgrass specimens for genetic identification of non-native aquatic plants in Florida. The protocol is directly related to the Lake Kissimmee Revegetation - Submerged Vegetation project being managed by the Florida Fish and Wildlife Conservation Commission. The sampling procedures outlined in the document establish quality control measures for eelgrass collection, requiring sample leaf blades to be cut from the plant base, dried on paper towels to remove debris and foreign material, labeled with collection details (locality, coordinates, date, collector name and contact information), trimmed to 3-4 inches in length, and individually placed in paper envelopes. Samples are then sealed in ziplock bags filled with silica beads to remove moisture during storage and transport. The document specifies that multiple samples can be combined in a single bag provided each leaf is maintained in a separate labeled envelope, with the quantity of silica beads adjusted accordingly to maintain proper desiccation.
Samples following this protocol are to be sent to the Aquatic and Wetland Plant Science Lab at Fort Lauderdale Research and Education Center (FLREC), University of Florida, located at 3205 College Avenue, Davie, FL 33314, with inquiries directed to Dr. Chad Keates (chadkeates@ufl.edu) or Dr. Lyn Gettys (lgettys@ufl.edu). This sampling protocol supports the Lake Kissimmee project's requirement for genetic authentication and screening of nursery-grown eelgrass (Vallisneria neotropicalis) to ensure plants are native species and prevent the introduction of non-native hybrid eelgrass varieties. The rigorous quality control procedures documented here serve as a foundational mechanism for validating the genetic purity of the approximately 75,000-100,000 eelgrass plant-pots (based on the $300,000 budget allocation) that bidders will supply for restoration activities at Lake Kissimmee in Polk County.
View the file
Other files
| File | Type | Posted |
|---|---|---|
| Attachment C_FINAL.pdf | ||
| FWC 25.26-38 FINAL Addendum #1 QA.pdf | ||
| Attachment D_FINAL.pdf | ||
| Attachment E_FINAL.pdf | ||
| FWC 25.26-38 FINAL.pdf |
On GovTribe
Work with this file
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
SEARCHING FOR EELGRASS
Sampling Protocol for the Identification of Non-native Aquatic Plants in Florida
SAMPLE PREPARATION
WHAT YOU WILL NEED
- Plastic Bag
- Silica Beads
- Paper Envelopes
- Scissors
- Marker
- Towel
- Phone/GPS
STEP 1
Cut a leaf blade off of the eelgrass, starting close to the base. Try your best to avoid older plant material. The better the sample, the easier it will be to extract DNA of high quality.
STEP 2
Dry the leaf on a paper towel, ensuring to remove any debris, algae or foreign plant material from the leaf cutting.
This will reduce the chance of contami-nation in downstream processing.
STEP 3
Write the sample details onto the paper envelope with the marker. In-clude the following: general locality, coordinates, date of collection, name of collector, and contact information of collector (in case we need more info).
‘Click the orange links for more details’ https://www.amazon.com/Dry-Desiccant-Indicating-Industry-Standard/dp/B01I5Y2DG6/ref=sxin_16_pa_sp_search_thematic_sspa?content-id=amzn1.sym.140400a7-1208-46ad-8d2a-eb6e8eac81b5%3Aamzn1.sym.140400a7-1208-46ad-8d2a-eb6e8eac81b5&cv_ct_cx=silica+gel+amazon&dib https://www.envelopes.com/brown-kraft-3-coin-2-1-2x4-1-4-envelopes-item-1623989
SAMPLE PREPARATION
STEP 5
Place the leaf cutting into the labelled envelope ensuring that you only place one leaf in the envelope. Close the envelope. You do not need to place silica beads in the envelope. Use envelopes that don’t retain water.
STEP 4
Ensure that the sample is 3 — 4 inches long using a ruler. Trim samples that are longer. Samples must not be longer than the envelope they are placed in otherwise they won’t dry properly.
STEP 6
Place the sealed envelope in a zip lock bag and fill the bag with silica beads.
Ensure that the bag is thoroughly filled so the envelope is covered. The silica beads remove moisture from bag so the more beads the better.
STEP 7
You can add multiple samples to the same bag containing silica beads. Just ensure that each leaf is in a separate envelope with unique sample infor-mation. The more samples you have the more silica beads you will need.
CONTACT INFORMATION
Where to Send Samples
Aquatic and Wetland Plant Science Lab
Fort Lauderdale Research and Education Center (FLREC)
University of Florida
3205 College Avenue
Davie, FL
33314
IF YOU HAVE ANY QUESTIONS
Dr Chad Keates — chadkeates@ufl.edu
Dr Lyn Gettys — lgettys@ufl.edu mailto:chadkeates@ufl.edu mailto:lgettys@ufl.edu
File details come from the government source that posted it. Updated .