Attachment A Bid Specifications.pdf

Attachment A - Bid Specification for DOH24-039P # Item Description Yes No

0 UHPLC-DAD UHPLC and Diode Array Detector capable of detecting domoic acid in shellfish tissues.

[ ] Yes [ ] No (fail)

1 Installation Requirements

Installation, Setup, Calibration, and Performance Verification shall be performed at PHL at no cost to the DOH.

[ ] Yes [ ] No (fail)

1 Discrimination UHPLC-DAD must be able to achieve baseline separation between the peaks of L-tryptophan and domoic acid (g)

[ ] Yes [ ] No (fail)

2 Sample Capacity Auto sampler with suitable injector capable of holding minimum 100 sample vials

[ ] Yes [ ] No (fail)

3 LOQ A limit of quantitation of 1 ppm of domoic acid in shellfish

[ ] Yes [ ] No (fail)

4 Sensitivity The instrument must be able to achieve a signal to noise ratio of 10:1 for a 40 ppb injection of domoic acid

[ ] Yes [ ] No (fail)

5 Reliability The instrument is expected to routinely perform sample measurements around the clock for months at a time without failure or required service. Routine recommended maintenance intervals should not exceed once every 6 months.

[ ] Yes [ ] No (fail)

6 Reliability (Up-time)

Instrument IS NOT prone to frequent breakdown AND is fully operational at least 90% of the time.

A reasonable standard applies.

[ ] Yes [ ] No (fail)

7 Service Agreement

Contractor shall provide an as needed option to the DOH a service agreement for the five (5) years in one year increments following expiration of the original warranty period.

[ ] Yes [ ] No (fail)

General System Requirements The required technical features of the system include:

8 Service Availability

The Contractor must be able to provide a service agreement that includes on-site response to service calls within three (3) business days.

[ ] Yes [ ] No (fail)

9 Extended Warranty

The Contractor must be able to provide an extended warranty for two years that also includes one preventative maintenance in the second year.

[ ] Yes [ ] No (fail)

10 Compatibility The UHPLC components must be compatible with an Agilent LC/MS Ultivo and 6475A in order to provide redundancy in the lab.

[ ] Yes [ ] No (fail)

11 Size Requirements

Due to laboratory space for installation, the footprint of the instrument is not to exceed a height of 35 inches (not including solvent bottles), a width of 18 inches, and a depth of 19 inches.

[ ] Yes [ ] No (fail)

1 Type: Quaternary [ ] Yes [ ] No (fail) 2 Delay volume Must have as low as possible gradient delay volume of the pump = 350 μL [ ] Yes [ ] No (fail)

3 Flow Rate Must be able to maintain an isocratic 1.0 mL/min up to 5.0/ml/min maximum flow rate.

[ ] Yes [ ] No (fail)

4 Operation Pressure Range

Must have operating range up to 1300 bar at 2 ml/min, and 800 bar at 5 mL/min.

[ ] Yes [ ] No (fail)

5 Solvent Channels

At least 4 Solvent Channels [ ] Yes [ ] No (fail)

6 pH Range Can tolerate pH range of 1.0 — 12.5 [ ] Yes [ ] No (fail) 7 Flow Precision Must have ≤0.07% RSD or ≤0.01 min SD, whatever is greater. This is included to provide a more accurated compound identification through precise retention time.

[ ] Yes [ ] No (fail)

8 Hydraulic System Dual pistons in series. [ ] Yes [ ] No (fail)

UUHPLC/UHPLC Specific Pump

Column Oven/Thermostat

1 Temperature control

• Column compartment with oven capable of accurate temperature control at 40°C for domoic acid analysis.

• Temperature accuracy must be ± 0.5°C with calibration

• Must have a temperature range from 10°C below ambient to 85°C.

• The module must enable pre column heating and post column cooling.

[ ] Yes [ ] No for any item (fail)

2 Independent Temperature Zones

Must have at least two independent temperature zones.

[ ] Yes [ ] No (fail)

3 Capacity Must be able to contain up to 4 columns with a length of 30 cm each.

[ ] Yes [ ] No (fail)

1 Mobile phase degassing unit. [ ] Yes [ ] No (fail)

1 Refrigerated Autosampler Sample in vials must be maintained at a temperature of 0-4 °C.

[ ] Yes [ ] No (fail)

2 Capacity Must be able to have various and scalable drawer types from 2-16 well plates all within the LC-stack footprint.

[ ] Yes [ ] No (fail)

3 Injection Volume • Loop suitable for twenty (20) μL injections.

• Adjustable range: 0.1 to 900μL volumes or 1800µL by seat loop extension .

[ ] Yes [ ] No for any item (fail)

4 Carryover Prevention

Must be capable of performing a multi-wash option.

[ ] Yes [ ] No (fail)

1 Type Diode Array- 1024 element preferred for highest spectral resolution.

[ ] Yes [ ] No (fail)

2 Wavelength range

• Wavelength range must be at least 190-950 nm, settable in 1 nm increments.

• Must be sensitive at wavelengths of 242 nm and 350 nm for Domoic acid detection.

[ ] Yes [ ] No for any item (fail)

3 Noise < ± 0.7 x 10-5 AU at 254 nm and at 750 nm (cell path length 10 mm, response time 2 s, flow 1 ml/min LC-grade.

[ ] Yes [ ] No (fail)

4 Light Source Dual lamp design (tungsten and deuterium).

[ ] Yes [ ] No (fail)

5 Drift Signal drift must be equal or smaller 0.9 x 10-3 AU/h after adequate warm up.

[ ] Yes [ ] No (fail)

Autosampler

DAD Detector

Degassing Unit

6 Maximum sampling rate

120 Hz [ ] Yes [ ] No (fail)

7 Linearity > 2 AU (upper limit). Important to allow the simultaneous determination of high and low levels of the analytes.

[ ] Yes [ ] No (fail)

8 Operating Temperature

The temperature operating range of instrument must be from 4°C to 55°C.

[ ] Yes [ ] No (fail)

9 Compatibility Compatibility with wide range of flow cells [ ] Yes [ ] No (fail) 10 RFID tags • Radio Frequency Identification Tags for flow cells and UV lamp to record parameters.

• The information needs to be saved with each individual raw data file for data traceability, minimizing the risk of false data interpretation

[ ] Yes [ ] No for any item (fail)

11 Number of signals

Simultaneous acquisition of up to 8 compound specific wavelength to provide increased sensitivity and selectivity.

[ ] Yes [ ] No (fail)

12 Slit Width • Programmable: 1, 2, 4, 8, 16 nm

• Must allow performance optimization regarding:

o Spectral resolution o Signal to noise (or limit of detection)

[ ] Yes [ ] No for any item (fail)

1 PC The PC is the latest available and is capable of running 64 bit MS Windows or greater.

[ ] Yes [ ] No (fail)

2 Network Interface Card

(NIC)

Is there a manufacturer installed NIC card?

Type: ______________________

[ ] Yes [ ] No (fail) No answer = fail

3 Analytical Software Requirements

Instrument must have software for both qualitative and quantitative analysis

[ ] Yes [ ] No (fail)

4 Instrument Control Software

The software that controls the operations of the instrument must have the statuses and control of individual modules displayed in the same window.

[ ] Yes [ ] No (fail)

System Computer

5 Quantitative Software

Quantitative software allows user to quickly and easily inspect chromatograms and area counts for multiple analytes for each calibration, quality control, and unknown sample. Software must meet this condition.

[ ] Yes [ ] No (fail)

6 Peak Integration Analysts must be able to manually integrate peaks on chromatograms quickly and easily.

Software must meet this condition.

[ ] Yes [ ] No (fail)

7 Method Editing The software must allow analyst to quickly reprocess batches and edit calibration concentrations and analyte retention times.

[ ] Yes [ ] No (fail)

8 Data Format The software must display the run data in a format that displays all quality control criteria for an analyte in one window for facilitated inspection and printing purposes.

[ ] Yes [ ] No (fail)

9 Data File Management

Data folder management must be efficient and organized in a way that all files from individual runs are placed into the same subfolder.

[ ] Yes [ ] No (fail)

10 Software Roadmap

Bidder must have a software road map for major/minor updated versions of the software. Please describe how the enhancements are handled.

[ ] Yes [ ] No (fail) No answer = fail

11 Service Packs Bidder must provide service packs. Please describe what is included in the service pack for the instrument.

[ ] Yes [ ] No (fail) No answer = fail

12 Data Back-up • The software must automatically backup the data on a reasonable periodic schedule.

• Please describe if it is it a full back-up or incremental back up and what is the back-up frequency?

[ ] Yes [ ] No (fail) No answer = fail

13 Data Overwriting Instrument must allow manual overwriting of the dataset.

[ ] Yes [ ] No (fail)

14 Internet Access • Does your customer support need access to the instrument via the internet?

• If Yes, Please describe.

[ ] Yes [ ] No (fail) If Yes, No answer = fail

15 Monitor • Does the software allow for touch screen capability?

Note: If yes, the bidder must provide a monitor that also has touchscreen.

• Is the monitor an LED type

• Is the size is 24 inch or larger?

[ ] Yes [ ] Yes [ ] Yes

[ ] No aaaaaaaaaaaaaaa aaaaaaaaaaaaaaa aaaaaa [ ] No aaaaaaaa [ ] No (fail)

16 LIMS System Capability

Is the instrument capable of connecting to a Laboratory Information System (LIMS) to be able to transfer data to a lab reporting system?

[ ] Yes [ ] No

1 Hands -on Training

Hands-on Training is provided at the customer site and includes at least 8 hours of instructor led and hands-on training provided at the customer site by a service engineer for 2 to 4 personnel

• Instrument orientation

• UHPLC/DAD Operation

• Method Set-up

• Instrument Preventative maintenance.

• Trouble shooting

• Method development

• Quick reference guides or other training materials

[ ] Yes [ ] No (fail)

2 Software Training A minimum 6 hrs. of Instructor Led Software orientation & training for up to 4 personnel.

[ ] Yes [ ] No (fail)

Training

Notes

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