A04_Specifications 1232SA26Q0940.docx

United States Department of Agriculture Research, Education, and Economics Agricultural Research Service

Specification Document

1173628_Cyrotanks, and Racking System for NLGRP

06/26/2026

Agricultural Genetic Resources Preservation Research Fort Collins, Colorado

TABLE OF CONTENTS

SECTION

01 Need statement

02 Required Specification

SECTION 01 Need Statement

USDA ARS CARR AGRPRU request 7 cryogenic freezers to continue research.

1. Mission Need The NLGRP maintains long-term cryogenic storage of critical genetic resources, including plant, animal, and microbial germplasm essential to USDA research, food security, and global agricultural preservation efforts. Reliable, liquid and vapor-phase cryogenic storage at -150 to –190°C using liquid nitrogen is required to ensure viability and long-term stability of irreplaceable plant and animal germplasm. Failure of storage equipment will result in irreversible loss of national genetic resources assets.

Brand-Name Justification Request to Specify Brand Name: MVE HEco / HE 1879P-190AF-GB (PN 20681339)

1. Compatibility Requirements NLGRP’s existing cryogenic infrastructure is standardized around MVE 1800-series geometry and systems. Switching brands would require major replacement of racks, SOP revisions, freezer monitoring systems, and retraining.

2. Unique Technical Features

- CryoVerse™ Connect controller with data logging, security, and remote monitoring.

- Gas Bypass Automatic Fill System minimizing temperature fluctuations.

- Proven –190°C vapor-phase and -190°C liquid nitrogen uniformity for sensitive storage.

3. Risk Avoidance Standardization prevents inconsistent storage conditions and supports continuity-of-operations planning.

4. Total Cost of Ownership MVE HEco systems offer reduced LN₂ usage and long-term maintainability, lowering lifetime costs.

Conclusion: The MVE 1879P-190AF-GB is the only model that meets NLGRP’s critical cryogenic storage requirements.

END OF SECTION

SECTION 02 Required Specifications

The following are the specifications for cryogenic freezers used at the National Laboratory for Genetic Resources Preservation (NLGRP): 6- MVE HECO 1842P-150AF-GB FULL AUTO, GB PN# 13817440 Manufacture Specifications:

Must have user defined alarms

Controller must be able to monitor temperatures both in liquid and vapor phase Controller must have the ability to autofill the tank or to setup a timed schedule fill of the tank

Tank Capacity:

The following are the specifications for cryogenic freezers used at the National Laboratory for Genetic Resources Preservation (NLGRP): 1- MVE HECO 1879P-190AF-GB FULL AUTO, GB PN# 20681339 Manufacture Specifications:

Manufacture Specifications:

Stainless steel construction Nitrogen use only Double walled vacuum insulation with insulating material in the vacuumed space Vacuumed space should have chemicals added to assist with vacuum maintenance Casters for mobility of tank 25” neck opening Usable internal height of 29.5” Inner diameter of 56.0” Overall height with a controller unit of 63.7” Width of 60” Weight of single unit 1721lbs empty Weight of a single unit 4830lbs full UL Listed for 110-208 volts .73 Amps at 110 volts All piping must be brass rated at 35psi Stainless steel, reenforced, ½” fill hose of 6 feet long Fill hose must connect directly to a ½” flare connection Tank piping must have solenoid on/off controls Tank piping must have a 50psig pressure relief valve Tank piping must have brass inline filter of 40micron mesh Tank must have hot gas bypass ability There must be the ability to manually measure liquid level in inches Must have TEC 3000 controller Must have password protection ability Must have user defined alarms Controller must be compatible with Johnson Controls Metysis system and MODBUS system Must be able to maintain -150° C in Nitrogen vapor phase Must have the ability to store samples in liquid or vapor phase Controller must be able to measure liquid levels in inches Controller must be able to monitor temperatures both in liquid and vapor phase Controller must have the ability to autofill the tank or to setup a timed schedule fill of the tank Tanks must have a single level rotating tray for storage canisters Lid must have a Styrofoam plug Static loss rate of no more than 1.6L/day Tank Capacity:

LN2 Capacity at Vapor Platform L est. 292 LN2 Capacity w/o Inventory L est. 1516

Maximum storage capacity:

1.2 & 2 ml Vials (Internally Threaded) 79,950 Quantity of Large Racks 100 cell boxes 54 Quantity of Mini Racks 25 cell boxes 30 Number of Shelves per Rack 13

National Laboratory for Genetic Resources Preservation 1111 South Mason Street Fort Collins, CO 80521 An Equal Opportunity Employee image1.png image2.png