C04-4 Appendix II Sabana Field Research Station - Sample handling and processing room rev1.pdf

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Environmental Services: Hazardous Material Inventory, Removal, Disposal
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This document is a chemical inventory and hazard management record for the Sabana Field Research Station's sample handling and processing room.

The inventory catalogues approximately 80+ chemicals stored at the facility, including hydrochloric acid (in various concentrations and grades), potassium compounds, ammonium fluoride, sodium hydroxide, magnesium perchlorate, compressed gases (nitrogen and carbon dioxide), and specialized reagents such as pH buffers and calibration standards. Chemicals are stored in containers ranging from 0.75 mL to 10,000 mL, with most stored in either Nalgene polypropylene or glass containers. Storage locations are designated as either "wet side" or "dry side" with specific cabinet assignments (flammable cabinets, corrosives cabinet, pesticides cabinet, or bench-top desiccators). The inventory provides chemical names, CAS numbers, physical states (liquid, solid, or gas), current volume quantities, container volumes, and Safety Data Sheet (SDS) locations with hyperlinks to external resources. Primary contacts for chemical management are Tana Wood and Tatiana Barreto, with secondary contacts including Whendee Silver, Stephen Porter, and Iana Grullón Penkova. The document serves as a comprehensive reference tool for laboratory safety protocols, hazard awareness, and chemical accountability at the research facility.

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Chemical Name Additional Description Container Type State (Liquid or Solid) Volume Quantity Container Volume SDS Location Contact Person

Isopropanol 70% Isopropyl alcohol 70% in water with soil residue Nalgene PP Liquid <100 ml 1000 ml https://www.fishersci.ca/shop/products/ipa-isopropyl-alcohol-70-v-v-fisher-chemical-4/a4591#tab23

Flammable cabinet by fume hood

Tana Wood/Tatiana Barreto pH buffers, 1M NH4F Ammonium fluoride, 0.5M HCl Hydrochloric acid

The chemicals in the previous column are combined in the same bottle. pH buffer solutions 4, 7, 10 Mettler Toledo mixed together with Bray (NH4F + HCl). To prepare 1M NH4F we combined 3.7 g of NH4F in 100 mL of DI water.

To prepare the 0.5M HCl we used 20.7 mL of concentrated HCl (12.1 N) on 500 mL of DI water.

Nalgene PP Liquid ~400 ml 1000 ml https://assets.fishersci.com/TFS-

Assets/CCG/EU/SDS/SDS_10201841_EN.pdf Cabinet located below the broken fume hood Tana Wood/Tatiana Barreto

0.5 M K2SO4 Pottasium sulfate, 1M

NH4F Ammonium fluoride, 0.5M

HCl Hydrochloric acid

The chemicals in the previous column are combined in the same bottle. To prepare 1M

NH4F we combined 3.7 g of NH4F in 100 mL of DI water; to prepare 0.5M K2SO4 we put 174.2 g of K2SO4 to 2.0 liters of DI water; and to prepare the 0.5M HCl we used 20.7 mL of concentrated

HCl (12.1 N) on 500 mL of DI water.

Nalgene PP Liquid 1000 ml 1000 ml

1) https://www.fishersci.com/store/msds?partNumber=A665100&productDe scription=AMMONIUM+FLUORIDE+CERT+100+GM&vendorId=VN00033897

&countryCode=US&language=en; 2) https://pim-resources.coleparmer.com/sds/19590.pdf; 3) https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

0.5 M K2SO4 Pottasium sulfate, 2M

KCl Pottasium Chloride, 1M NH4F

Ammonium Fluoride, 0.5M HCl Hydrochloric acid

The chemicals in the previous column are combined in the same bottle. To prepare 1M

NH4F we combined 3.7 g of NH4F in 100 mL of DI water; to prepare 2M KCl we put 298.2 g of KCl to 2.0 liters of DI water; to prepare 0.5M

K2SO4 we put 174.2 g of K2SO4 to 2.0 liters of DI water; and to prepare the 0.5M HCl we used

20.7 mL of concentrated HCl (12.1 N) on 500 mL of DI water.

Nalgene PP Liquid 1000 ml 1000 ml

1) https://www.fishersci.com/store/msds?partNumber=A665100&productDe scription=AMMONIUM+FLUORIDE+CERT+100+GM&vendorId=VN00033897

&countryCode=US&language=en; 2) https://www.fishersci.com/store/msds?partNumber=P330500&productDe scription=POT+CHLORIDE+USP%2FFCC+500G&vendorId=VN00033897&c ountryCode=US&language=en ; 3) https://pim-resources.coleparmer.com/sds/19590.pdf; 4) https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

0.5 M K2SO4 Pottasium sulfate, 2M

KCl Pottasium Chloride, 1M NH4F

Ammonium Fluoride, 0.5M HCl Hydrochloric acid

The chemicals in the previous column are combined in the same bottle. To prepare 1M

NH4F we combined 3.7 g of NH4F in 100 mL of DI water; to prepare 2M KCl we put 298.2 g of KCl to 2.0 liters of DI water; to prepare 0.5M

K2SO4 we put 174.2 g of K2SO4 to 2.0 liters of DI water; and to prepare the 0.5M HCl we used

20.7 mL of concentrated HCl (12.1 N) on 500 mL of DI water.

Nalgene PP Liquid 1000 ml 1000 ml

1) https://www.fishersci.com/store/msds?partNumber=A665100&productDe scription=AMMONIUM+FLUORIDE+CERT+100+GM&vendorId=VN00033897

&countryCode=US&language=en; 2) https://www.fishersci.com/store/msds?partNumber=P330500&productDe scription=POT+CHLORIDE+USP%2FFCC+500G&vendorId=VN00033897&c ountryCode=US&language=en ; 3) https://pim-resources.coleparmer.com/sds/19590.pdf; 4) https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

12.1 N HCl Hydrochloric acid

Hydrochloric Acid, Certified ACS Plus, 36.5 to

38.0%, Fisher Chemical™ Glass Liquid <100 ml 500 ml https://www.fishersci.ca/store/msds?partNumber=A144S500&productDes cription=hydrochloric-acid-certified-acs-plus-fisher-chemical-

10&language=en&countryCode=CA

Cabinet located below the fume hood

Stephen Porter

0.5 N HCl Hydrochloric acid

To prepare the 0.5 N HCl we used 20.7 mL of concentrated HCl (12.1 N) on 500 mL of DI water.

Nalgene PP Liquid ~200 1000 ml https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

10% HCl Hydrochloric acid 12.1 N hydrochloric acid solution in water Glass - Amber Liquid 3800 ml 3800 ml https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

10% HCl Hydrochloric acid 12.1 N hydrochloric acid solution in water Glass - Amber Liquid 3800 ml 3800 ml https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

10% HCl Hydrochloric acid 12.1 N hydrochloric acid solution in water Glass Liquid 2500 ml 2500 ml https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

10% HCl Hydrochloric acid 12.1 N hydrochloric acid solution in water Glass Liquid 1500 ml 2500 ml https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

10% HCl Hydrochloric acid 12.1 N hydrochloric acid solution in water Plastic Liquid 4000 ml 4000 ml https://www.fishersci.com/store/msds?partNumber=A144C212&countryC ode=US&language=en

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

Trace Metal grade HCl Hydrochloric acid

Hydrochloric Acid (TraceMetal™ Grade), Fisher Chemical

Glass Liquid 250 ml 500 ml https://www.fishersci.ca/store/msds?partNumber=A508P500&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical- 6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Whendee Silver

Trace Metal grade HCl Hydrochloric acid

Hydrochloric Acid (TraceMetal™ Grade), Fisher Chemical

Glass Liquid 350 ml 500 ml https://www.fishersci.ca/store/msds?partNumber=A508P500&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical- 6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Whendee Silver

2N HCl Hydrochloric acid Trace metal grade hydrochloric acid solution in water Nalgene PP Liquid 1000 ml 1000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical-

6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

2N HCl Hydrochloric acid Trace metal grade hydrochloric acid solution in water Nalgene PP Liquid 1000 ml 1000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical-

6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

2N HCl Hydrochloric acid Trace metal grade hydrochloric acid solution in water Nalgene PP Liquid 500 ml 1000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical-

6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

2N HCl Hydrochloric acid Trace metal grade hydrochloric acid solution in water Nalgene PP Liquid 1000 ml 1000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical-

6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

2N HCl Hydrochloric acid Trace metal grade hydrochloric acid solution in water Nalgene PP Liquid 2000 ml 2000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical-

6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

2N HCl Hydrochloric acid Trace metal grade hydrochloric acid solution in water Glass Liquid 2500 ml 2500 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical-

6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Tatiana Barreto

2N HCl Hydrochloric acid, diluted to unknown concentration

Trace metal grade hydrochloric acid solution in water. Originally was 2N HCl, but was diluted to unknown concentration in the process of cleaning leaves.

Plastic carboy Liquid 10,000 ml 10,000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical- 6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Avner Gross

2N HCl Hydrochloric acid, diluted to unknown concentration

Trace metal grade hydrochloric acid solution in water. Originally was 2N HCl, but was diluted to unknown concentration in the process of cleaning leaves.

Nalgene PP Liquid 1000 ml 1000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical- 6&language=en&countryCode=CA&keyword=true

Cabinet located below the fume hood

Tana Wood/Avner Gross

2N HCl Hydrochloric acid, diluted to unknown concentration

Trace metal grade hydrochloric acid solution in water. Originally was 2N HCl, but was diluted to unknown concentration in the process of cleaning leaves.

Plastic carboy Liquid 4000 ml 4000 ml https://www.fishersci.ca/store/msds?partNumber=A508P212&productDes cription=hydrochloric-acid-tracemetal-grade-fisher-chemical- 6&language=en&countryCode=CA&keyword=true

Flammable cabinet by door Tana Wood/Avner Gross pH calibration standards (4.00) Expired calibration standard Plastic commercial bottle

Liquid

0.75 mL 0.75 mL

Wet side, upper cabinet Iana Grullón Penkova pH calibration standards (7.00) Expired calibration standard Plastic commercial bottle

Liquid

0.75 mL 0.75 mL

Wet side, upper cabinet Iana Grullón Penkova pH calibration standards (10.00) Expired calibration standard Plastic commercial bottle

Liquid

0.75 mL 0.75 mL

Wet side, upper cabinet Iana Grullón Penkova

1 x PBS 0.05% Tween-20 7732-18-5/7647-14-5/7558-794 50-00-0/9005- 64-5/7447-40-7/7778-77-0/1310-73-2 Liquid (L) 1 L 1 L Dry side, Fridge 1

Tana Wood/Tatiana Barreto

1 x PBS 0.05% Tween-20 7732-18-5/7647-14-5/7558-794 50-00-0/9005- 64-5/7447-40-7/7778-77-0/1310-73-2 Liquid (L) 1 L 1 L Wet side

Tana Wood/Tatiana Barreto

Abound Flowable Fungicide 131860-33-8/57-55-6/9084-06-4 Liquid (L) 1 gallon 1 gallon Dry side, Pesticides cabinet Tana Wood/Tatiana Barreto

Agarose 9012-36-6 Solid (S) 100 g 100 g Wet side Tana Wood/Tatiana Barreto Ammonium Fluoride 12125-01-8 Solid (S) 100 g 100 g Wet side Tana Wood/Tatiana Barreto

Banrot 40 WP (40% Wettable Powder)23564-05-8/2593-15-9 Solid (S) 2 lb (907 g) 2 lb (907 g) Dry side, Pesticides cabinet Tana Wood/Tatiana Barreto

Calcium Carbide 72-82% 75-20-7/1305-78-8 Solid (S) 2.5 kg 2.5 kg Dry side, Flammable Cabinet # 1

Tana Wood/Tatiana Barreto

Calcium Chloride 10035-04-8 Solid (S) unknown unknown Dry side, bench-top desicator Tana Wood/Tatiana Barreto

Carbon dioxide (Compressed gas) 124-38-9 Gas (G) 12 g 12 g Wet side Tana Wood/Tatiana Barreto

Chloroform (Amylene Stabilized) Chromasolve 67-66-3 Liquid (L) 1 L 1 L Dry side, Flammable Cabinet # 1

Tana Wood/Tatiana Barreto

D(+) Glucose (Dextrose) Anhydrous 50-99-7 Solid (S) 500 g 500 g Wet side Tana Wood/Tatiana Barreto

Hydrochloric Acid 7732-18-5/7647-01-0 Liquid (L) 2.5 L 2.5 L Dry side, Corrosives cabinet Tana Wood/Tatiana Barreto

Appendix II Sabana Field Research Station - Sample handling and processing room

Hydrochloric Acid (10 %) 7647-01-0 Liquid (L) 4 L 4 L Dry side, Corrosives cabinet Tana Wood/Tatiana Barreto

Hydrochloric Acid (2 N) 7647-01-0/7732-18-5 Liquid (L) 4 L 4 L Dry side, Corrosives cabinet Tana Wood/Tatiana Barreto

Hydrochloric Acid (2 N) 7647-01-0/7732-18-5 Liquid (L) 1 L 1 L Dry side, Corrosives cabinet Tana Wood/Tatiana Barreto

Hydrochloric Acid (Trace Metal Grade)7647-01-0 Liquid (L) 2.5 L 2.5 L Dry side, Corrosives cabinet Tana Wood/Tatiana Barreto

Magnesium Perchlorate (Anhydrous) 10034-81-8 Solid (S) unknown unknown Dry side, bench-top desicator Tana Wood/Tatiana Barreto

Magnesium Perchlorate (Anhydrous) 10034-81-8 Solid (S) 500 g 500 g Wet side Tana Wood/Tatiana Barreto Nitrogen (compressed gas) 7727-37-9 Gas (G) K cylinder K cylinder Wet side, closet Tana Wood/Tatiana Barreto Nitrogen (compressed gas) 7727-37-9 Gas (G) unknown unknown Wet side, closet Tana Wood/Tatiana Barreto Orchid Plus Fertilizer 6484-52-2/15708-41-5/10043-35-3 Solid (S) 16 oz (453.59 g) 16 oz (453.59 g) Wet side Tana Wood/Tatiana Barreto Phenolphthalein AR (powder) 77-09-8 Solid (S) unknown unknown Wet side Tana Wood/Tatiana Barreto Potassium Chloride 7447-40-7 Solid (S) 3 kg 3 kg Wet side Tana Wood/Tatiana Barreto Potassium Chloride 7447-40-7 Solid (S) 500 g 500 g Wet side Tana Wood/Tatiana Barreto Potassium Chloride 7447-40-7 Solid (S) 2.5 kg 2.5 kg Wet side Tana Wood/Tatiana Barreto Potassium Hydroxide 13110-58-3 Solid (S) 500 g 500 g Wet side Tana Wood/Tatiana Barreto Potassium Sulfate 7778-80-5 Solid (S) 500 g 500 g Wet side Tana Wood/Tatiana Barreto Sodium Bicarbonate (powder) 144-55-8 Solid (S) 500 g 500 g Wet side Tana Wood/Tatiana Barreto Sodium Hydroxide (pellets) 13110-73-2/497-19-8 Solid (S) 1 kg 1 kg Wet side Tana Wood/Tatiana Barreto Sodium Phosphate Dibasic (Anhydrous)7558-79-4 Solid (S) 500 g 500 g Wet side Tana Wood/Tatiana Barreto

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