Neuropixels opto: integrated silicon probes for cell-type-specific electrophysiology
Active Cooperative Agreement Awarded Last action
- Award ID
- U01NS133760
- Recipient
-
Allen Institute UEI NFHEUCKBFMU4
- Awarding agency
-
National Institute of Neurological Disorders and Stroke
National Institutes of Health, Department of Health and Human Services
- Obligated
- $1,312,227
Award facts
- Assistance listing
- 93.372 21st Century Cures Act - Brain Research through Advancing Innovative Neurotechnologies
- Completion date 81% complete
- Place of performance
- Seattle, WA 98109, USA
Award details come from USAspending.gov.
Description
- Abstract
- Within every brain region, neurons can be classified into dozens or hundreds of different cell types, each with unique functional roles and unique impacts on disease states. Traditionally, in vivo electrophysiological recordings-which have made invaluable contributions to our understanding of the neural basis of behavior- have not been able to distinguish the activity of genetically defined cell types. Despite recent advances in our ability to measure action potentials from many neurons simultaneously, IT remains difficult to connect these spike trains to underlying cell types and all that is known about their morphology, connectivity patterns, and intrinsic properties. Here, we propose to extend the widely used neuropixels platform by creating a version of these probes that is capable of both high-density electrophysiological recording and multi-color light delivery. This device, called neuropixels opto, could be used to identify cell types through an approach known as "optotagging," while also facilitating precise manipulations of genetically defined neural populations. We will work with imec, a nanoelectronics r & d organization with exclusive access to world-class fabrication facilities, to design and build a fully integrated implantable recording device with 1280 electrical readout sites, up to 48 red light emitters, and up to 62 blue light emitters. A proof-of-concept version of this probe has already been delivered to the allen institute, where IT was successfully used in an optotagging experiment. However, more work is required to develop a device that can be manufactured at scale and sold for around $2500/probe, in order to facilitate its dissemination throughout the systems neuroscience community. In collaboration with three external test sites, we will validate the efficacy of neuropixels opto for performing cell-type-specific recordings and manipulations in four brain regions: the visual cortex, entorhinal cortex, striatum, and the ventral tegmental area. In addition, we will extend popular data acquisition packages by developing modules for controlling these probes, as well as create new transgenic mouse lines that make IT much simpler for users to carry out dual-color optogenetic manipulations. Taken together, these efforts will make neuropixels opto a powerful, accessible, and indispensable tool for understanding the role that different cell types play in living brains.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $19,754 | |
| Amendment | $621,947 | |
| Original award | $670,526 |
Subawards
| Subrecipient | Amount | Awarded |
|---|---|---|
| The Trustees Of The University Of Pennsylvania | $60,000 | |
| Interuniversitair Micro-Electronica Centrum | $1,034,000 |
Quick answers
Who received award U01NS133760?
U01NS133760 was awarded to Allen Institute by National Institute of Neurological Disorders and Stroke (National Institutes of Health) on August 18, 2023.
How much is award U01NS133760 worth?
$1,312,227 has been obligated on U01NS133760.
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