Purchase Order 140G0125P0068

The U.S. Geological Survey's Columbia Environmental Research Center (CERC) awarded a $23,595 firm-fixed-price purchase order contract to Invivo Biosystems, Inc. (doing business as Nemametrix, Inc.), a small disadvantaged business, to generate a thiaminase-knockout zebrafish line. This contract is a 100% small business set-aside and supports a 5-year Department of Defense Strategic Environmental Research and Development Program grant focused on studying thiaminase production in fish. The contractor will complete critical phases including CRISPR design, F0 injections, and identification of germline transmission to produce the required zebrafish line. The work will be performed entirely at the contractor's facility in Eugene, Oregon over a 24-month period, with the final zebrafish line to be delivered to CERC in Columbia, Missouri by January 2026.