Transcriptome Services of RNA samples

Closed Solicitation Posted

This opportunity was awarded. See the award notice from .

Solicitation number
1232SA26Q0705
Agency
Responses due
Set-aside
Total Small Business

Opportunity facts

Place of performance
Beltsville, Maryland 20705, United States

Notice details come from SAM.gov. Updated .

About this opportunity

The Department of Agriculture Agricultural Research Service seeks transcriptome services for 110 RNA samples from intestinal tissues of sheep and rodents, with quotations due July 6, 2026. This is a Total Small Business Set-Aside (small business size standard $19 million), issued as a combined synopsis and RFQ under FAR Part 12 with no separate written solicitation to follow.

Place of performance is Calverton, Maryland. The NAICS code is 541380, Testing Laboratories and Services.

Samples are stored in TRIzol and classified as Biosafety Level 2 due to potential parasite content. Each sample must contain sufficient tissue to yield greater than 100 micrograms of total RNA (approximately 2 centimeters of intestine). The contractor shall perform next-generation sequencing using an Illumina NovoSeq X sequencer or equal platform, with rRNA depletion (preferred) or polyA mRNA enrichment applied consistently across all samples. Strand-specific sequencing at 25 to 40 million paired-end reads per sample is required. Deliverables include quality control reports, alignment files (BAM), expression count tables, FPKM tables, differential expression results with annotations, software versions and parameters, standard visualizations (PCA plots, heatmaps, volcano plots), and raw sequence data deposited to NCBI SRA under the principal investigator's instruction. All unused RNA samples must be returned to USDA in dry ice with USDA covering return shipping costs.

The contract structure comprises one base year (CLIN 0001) and two one-year option periods (CLINs 1001 and 2001), each requiring 110 samples. The period of performance runs August 25, 2026 through August 24, 2029. Award is firm-fixed-price on a lowest-price technically acceptable basis; only the lowest-priced offer will be evaluated for technical acceptability. Technical evaluation criteria include ability to meet the 60-day delivery schedule and demonstrated sequencing methodology. A 90-calendar-day price hold is required. The contracting officer is Amber Sampson; the contract specialist is Kerrie Hodges (kerrie.hodges@usda.gov). Questions and answers were posted June 17, 18, and 23, 2026.

Notice text

4 versions

Update #4 · Latest ·

***Questions and Answers posted in Attachments on 6/23/2026***

**Questions and Answers posted in Attachments on 6/18/2026**

*Questions and Answers posted in Attachments on 6/17/2026*

This is a combined synopsis/solicitation for a commercial service prepared in accordance with the format in FAR Part 12.  This announcement constitutes the only solicitation.  Quotations are being requested and a separate written solicitation will not be issued. 

Solicitation number 1232SA26Q0705 is issued as a Request for Quotation (RFQ) for Transcriptome Services of RNA samples for United States Department of Agriculture, Agriculture Research Service.

This acquisition is set-aside for small business concerns.  The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services  The small business size standard is $19 million.  This acquisition is a Total Small Business Set-Aside.  All responsible sources may submit a quotation which will be considered by the agency. 

Update #3 ·

**Questions and Answers posted in Attachments on 6/18/2026**

*Questions and Answers posted in Attachments on 6/17/2026*

This is a combined synopsis/solicitation for a commercial service prepared in accordance with the format in FAR Part 12.  This announcement constitutes the only solicitation.  Quotations are being requested and a separate written solicitation will not be issued. 

Solicitation number 1232SA26Q0705 is issued as a Request for Quotation (RFQ) for Transcriptome Services of RNA samples for United States Department of Agriculture, Agriculture Research Service.

This acquisition is set-aside for small business concerns.  The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services  The small business size standard is $19 million.  This acquisition is a Total Small Business Set-Aside.  All responsible sources may submit a quotation which will be considered by the agency. 

Update #2 ·

*Questions and Answers posted in Attachments on 6/17/2026*

This is a combined synopsis/solicitation for a commercial service prepared in accordance with the format in FAR Part 12.  This announcement constitutes the only solicitation.  Quotations are being requested and a separate written solicitation will not be issued. 

Solicitation number 1232SA26Q0705 is issued as a Request for Quotation (RFQ) for Transcriptome Services of RNA samples for United States Department of Agriculture, Agriculture Research Service.

This acquisition is set-aside for small business concerns.  The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services  The small business size standard is $19 million.  This acquisition is a Total Small Business Set-Aside.  All responsible sources may submit a quotation which will be considered by the agency. 

Update #1 ·

This is a combined synopsis/solicitation for a commercial service prepared in accordance with the format in FAR Part 12.  This announcement constitutes the only solicitation.  Quotations are being requested and a separate written solicitation will not be issued. 

Solicitation number 1232SA26Q0705 is issued as a Request for Quotation (RFQ) for Transcriptome Services of RNA samples for United States Department of Agriculture, Agriculture Research Service.

This acquisition is set-aside for small business concerns.  The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services  The small business size standard is $19 million.  This acquisition is a Total Small Business Set-Aside.  All responsible sources may submit a quotation which will be considered by the agency. 

Files

File Type Posted
Questions and Answers 23 June.pdf PDF
Questions and Answers 18 June.pdf PDF
Questions and Answers 17 June.pdf PDF
Solicitation_1232SA26Q0705.pdf PDF

Notice history

Notice Type Posted
Transcriptome Services of RNA samples Award Award Notice
This notice Latest solicitation Solicitation

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