Sea Lice Genotyping

Closed Solicitation Posted

Solicitation number
Solicitation1232SA26Q1506
Agency
Responses due
Set-aside
Total Small Business

Opportunity facts

Place of performance
Bangor, Maine 04401, United States

Notice details come from SAM.gov. Updated .

About this opportunity

The USDA Agricultural Research Service seeks DNA extraction and single nucleotide polymorphism genotyping services for 960 sea lice (Lepeophtheirus salmonis) samples, with quotations due September 24, 2026 at 11:00 AM EST and questions due September 18, 2026 at 4:00 PM EST. This is a Total Small Business Set-Aside.

Place of performance is Bangor, Maine. Work extends from date of award through December 31, 2026. The contractor must process 1,344 sea lice tissue samples (preserved in ethanol) in increments of 96-well plates using the AXIOM LSAL200K 96-ARRAY genotyping format on the GeneTitan Instrument or equivalent, and deliver raw data files in .cel and PLINK formats (.ped/.map or .bed/.bim/.fam) within 60 days of sample delivery through secure electronic portals (Box, AWS, or SFTP). The contractor must supply all genotyping arrays and return unused tissue and DNA samples to the government.

Performance requirements include achieving single nucleotide polymorphism call rates of 97% or greater for passing samples, maintaining a minimum sample pass rate of 95%, implementing a fully integrated Laboratory Information Management System for sample tracking, and establishing quality control procedures to ensure sample and data integrity. NAICS code is 541380, Testing Laboratories and Services. The PSC code is B529, Special Studies/Analysis–Scientific Data.

Award will be made to the lowest-priced, technically acceptable offer with acceptable or neutral past performance based on a best-value evaluation. Offerors must maintain pricing firm for 90 calendar days from offer receipt date. Amendment 001, effective September 15, 2026, clarified the unit of measure as "ea.=samples" and requires acknowledgment of the amendment prior to the submission deadline.

Notice text

The USDA Agricultural Research Service requires DNA extraction from sea lice (Lepeophtheirus salmonis) and single nucleotide polymorphism (SNP) genotyping.  Lice tissue samples will consist of single pre-adult or adult animals that have been preserved in ethanol.   Sea lice DNA genotyping will be completed using AXIOM LSAL200K 96-ARRAY genotyping in the 96-well format (Thermo-Fisher SKU number 550522) on the GeneTitan Instrument or equal. Deliverables will include the genotyping data for all the samples and the raw data files in .cel format (CEL files) and PLINK format (either .ped and .map or .bed, .bim, and .fam).  Unused tissue samples and DNA samples will be sent back to the government.  Contractor shall provide all services, supplies, equipment and facilities necessary to provide the deliverable specified in accordance with the terms, conditions, and specifications contained in this document.         

Files

File Type Posted
Sol_1232SA26Q1506_Amd_0001.pdf PDF
corrected - Schedule of Items.pdf PDF
Sol RFQ 123SA23Q1506 ARS Sea Lice Genotyping.pdf PDF
Schedule of Items.pdf PDF

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