scRNA sequencing service

The National Institutes of Health under the Department of Health and Human Services is seeking contractors to provide single-cell RNA sequencing services through this pre-solicitation notice. The procurement requires a comprehensive microfluidics-based sequencing system that includes dead cell removal protocols to ensure high data quality and sample preparation workflows designed to maximize cell viability. Key technical specifications include achieving 80% of bases with quality scores of Q30 or higher for 2x150 bp sequencing, with a preferred average of 90% or higher. The system must incorporate barcoding cells and droplet isolation using droplet sequencing methods, along with semiautomated processing capabilities for surface marker enrichment and cell isolation. The sequencing workflow must encompass end repair, A-tailing, ligation, and PCR processes, while providing flexible sequencing depth with a capture rate exceeding 60%. Additionally, the system must be capable of profiling paired V(D)J transcripts with gene expression and library preparation, and contractors must provide a robust bioinformatics pipeline for visualization and cross-referencing of sequencing data. The deadline for this pre-solicitation is August 4, 2025.

This opportunity does not utilize any set-aside designations, making it open to all qualified contractors regardless of socioeconomic status. No information regarding potential incumbents, current contractors, award values, or budget ranges has been disclosed in this pre-solicitation notice. The technical requirements specify that the system must achieve a capture rate greater than 60%, which represents a key performance threshold for potential contractors. The notice does not provide specific quantities of sequencing runs or samples to be processed, nor does it establish a definitive timeline for service delivery. The brief response window of approximately six days from posting to the August 4, 2025 deadline suggests this may be preliminary market research or preparation for a forthcoming full solicitation. Additional details regarding contract scope, duration, and financial parameters will likely be provided in subsequent solicitation documents.