RNA Extraction and RNA Sequencing

Closed Solicitation Posted

Solicitation number
12305B25Q0034
Agency
Northeast Area Field Research Implementation and Information Delivery, Department of Agriculture
Responses due
Set-aside
Total Small Business

Opportunity facts

NAICS code
541380 Testing Laboratories and Services
PSC
B529 Special Studies/Analysis- Scientific Data
Place of performance
Kearneysville, West Virginia 25430, United States
Points of contact

Notice details come from SAM.gov. Updated .

About this opportunity

The United States Department of Agriculture (USDA) Agricultural Research Service's National Center for Cool and Cold-Water Aquaculture is seeking a contractor to perform total RNA extraction and mRNA sequencing on 120 frozen rainbow trout whole-body tissue samples. This acquisition is structured as a combined synopsis/solicitation for commercial products and services, utilizing Simplified Acquisition Procedures under FAR Part 13. The contract will be awarded using a Lowest Price Technically Acceptable (LPTA) methodology, meaning the government will select the lowest-priced quote that meets all technical specifications. Offerors must submit documentation demonstrating technical capability, a firm fixed-price quotation, and their Unique Entity ID by January 17, 2025 at 9:00 AM EST. All questions regarding the solicitation must be submitted in writing to Contracting Specialist Dilynn Rogers via email by January 14, 2025, with telephone inquiries not being accepted.

The solicitation is designated as a Total Small Business set-aside under NAICS code 541380, with a small business size standard of $19 Million. The performance location will be the USDA ARS NCCCWA facility in Kearneysville, West Virginia. Contractors must be capable of extracting total RNA with specific quality metrics, including a minimum concentration of 250ng/uL, RNA quality ratio between 1.8-2.0, and a RIN value above 7. The contract requires completion of all RNA extraction and sequencing services within 30 days of receiving samples, with samples to be provided within 60 days after contract award. Deliverables will include sequencing data and a comprehensive project report, to be transmitted via secure portal or external hard drives. Only one award will be made under this solicitation based on available funding, with the expectation that the selected contractor will provide all necessary services, supplies, equipment, and facilities to complete the scientific research project.

Notice text

       This is a combined synopsis/solicitation for commercial products and commercial services prepared in accordance with the format in subpart 12.6, and is being conducted according to FAR Part 13, Simplified Acquisition Procedures. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued.

                (ii) The solicitation number and a statement that the solicitation is issued as a request for quotation (RFQ).

                (iii) A statement that the solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular FAR FAC 2025-06 January 3, 2025.

                (iv) This is a Total Small Business set aside and the associated NAICS code is 541380 and the small business size standard is $19 Million.

                (v) See attached document Statement of Work.

                (vi) USDA, Agriculture Research Service (ARS), National Center for Cool and Cold-Water Aquaculture (NCCCWA) requires total RNA extraction and mRNA sequencing of 120frozensamples ofrainbow trout whole-body tissue. Contractor shall provide all services, supplies, equipment and facilities necessary for total RNA extraction and mRNA sequencing of 120frozensamples ofrainbow trout whole-body tissue homogenized in Trizol.

*See attached Statement Work for additional information*  

      (vii) Performance location: USDA, ARS, NCCCWA, 11861 Leetown Rd, Kearneysville, WV 25430.

                (viii) Provision at 52.212-1, Instructions to Offerors—Commercial Products and Commercial Services, applies to this acquisition.

                (ix) Provision at 52.212-2, Evaluation-Commercial Products and Commercial Services, applies to this acquisition. The Government intends to award a Firm Fixed-Price (FFP) contract. The award will be made on the basis of Lowest Price Technically Acceptable (LPTA). "LPTA" means, the expected outcome of the acquisition, that results from the selection of the lowest evaluated price (evaluated as total cost of all line items) is a technically acceptable quotation, that fully meets all specifications listed. Only the lowest priced quote will be evaluated for technical acceptability (based on the specifications). The next lowest offer will only be evaluated if the aforementioned quote is not found technically acceptable (and so on). Only one award will be made under this solicitation based on availability of funding.

In order to be considered for award OFFERORS SHALL SUBMIT THE FOLLOWING:

(a)        Submit documentation showing technical capability to meet or exceed Performance Elements and Benchmarks in the Statement of Work.

(b)        Submit Firm Fixed Price quotation (quote on company letterhead) detailing the item description, total price and valid for at least 60 days after receipt of quote. Item delivered FOB Destination inclusive of all costs.

(c)        Submit Unique Entity ID (UEI) with quotation.

REJECTION OF QUOTATION: Failure to demonstrate compliance will be cause to reject the quote without further discussions. All responsible sources may submit an offer which will be considered.

                (x) Offers must have a completed copy of the provision at 52.212-3, Offeror Representations and Certifications-Commercial Products and Commercial Services, uploaded on Sam.gov by the time quotes are due.

                (xi) The clause at 52.212-4, Contract Terms and Conditions-Commercial Products and Commercial Services, applies to this acquisition.

                (xii) The clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders—Commercial Products and Commercial Services, applies to this acquisition. See attached clauses for additional applicable clauses.

                (xiv) All questions regarding this announcement must be submitted in writing to the Contracting Specialist, via email to dilynn.rogers@usda.gov by 14 January 2025. Telephone requests for information will not be accepted or returned. Quotes are due on 17 January 2025 NLT 9am EST by email to, Dilynn Rogers at dilynn.rogers@usda.gov.

DISCUSSIONS: The Government intends to award without discussions but reserves the right to conduct discussions should it be deemed in the Government's best interest.

Attachments

Files attached to this notice, newest first
File Type Posted
Statement of Work.pdf PDF
Clauses_Provisions.pdf PDF
WD.pdf PDF

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