Purchase of Illumina MiSeq System

***NOAA'S ACQUISITION & GRANTS OFFICE, WESTERN ACQUISITION DIVISION-BOULDER, NATIONAL MARINE FISHERIES SERVICE (NMFS), NORTHWEST FISHERIES SCIENCE CENTER (NWFSC), CONSERVATION BIOLOGY DIVISION (CBD) INTENDS TO NEGOTIATE, ON A SOLE SOURCE BASIS, UNDER AUTHORITY OF FAR 13.106-1(b)(1), WITH ILLUMINA, INC OF SAN DIEGO, CA FOR AN ILLUMINA MISEQ SYSTEM.

***THE SOLE SOURCE DETERMINATION IS BASED ON THE CBD'S REQUIREMENT FOR THE SAME MACHINE TO GENERATE SEQUENCING LIBRARIES. THE METHODS AND REAGENTS USED FOR GENERATING THE SEQUENCING LIBRARIES AND PERFORMING THE ACTUAL SEQUENCING REACTIONS ARE PARTICULAR TO THE TYPE (BRAND) OF INSTRUMENT USED. WITH COMPLEX PROCEDURES SUCH AS NEXT-GENERATION DNA SEQUENCING, DIFFERENCES IN REAGENTS, PROTOCOLS, OR INSTRUMENTS USED CAN CAUSE BIASES IN THE DATA. BIASES CAN BE MINIMIZED, HOWEVER, BY CONSISTENTLY USING THE SAME REAGENTS, PROTOCOLS AND INSTRUMENTS WITHIN AN EXPERIMENT. OUR REAGENTS AND PROTOCOLS WOULD THEN BE IDENTICAL FOR BOTH INSTRUMENTS, LARGE PROJECTS WOULD SAVE TIME BECAUSE SEQUENCING CAN BE DONE ON EITHER INSTRUMENT WHEN AVAILABLE AND ALL THE CBD'S DATA WILL BE INTERNALLY CONSISTENT AND CAN BE COMBINED WITHOUT CORRECTION FOR DIFFERENT BIASES. IN ADDITION, HIGH-THROUGHPUT SEQUENCING INSTRUMENTS ALSO GENERATE DATA IN PARTICULAR FORMATS, INDEXING (IDENTIFYING) SAMPLES IN SPECIFIC WAYS. THE CHEMISTRY THAT WE USE HAS BEEN CUSTOM MADE TO BE COMPATIBLE WITH THE MISEQ CHEMISTRY, BUT THAT CHEMISTRY USES PROPRIETARY REAGENTS THAT ARE NOT COMPATIBLE WITH OTHER COMPANIES' INSTRUMENTS. DELIVERY BY 10/31/2018.

***THIS NOTICE OF INTENT IS NOT A REQUEST FOR COMPETITIVE PROPOSALS AND NO SOLICITATION PACKAGE IS AVAILABLE. HOWEVER, FIRMS THAT BELIEVE THEY CAN FULLY MEET THE GOVERNMENT'S REQUIREMENTS MAY SUBMIT SUBSTANTIATING DOCUMENTATION IN WRITING TO THE IDENTIFIED POINT OF CONTACT WITHIN 15 DAYS AFTER PUBLICATION OF THIS SYNOPSIS. SUCH DOCUMENTATION WILL BE EVALUATED SOLELY FOR THE PURPOSE OF DETERMINING WHETHER OR NOT TO CONDUCT THIS PROCUREMENT ON A COMPETITIVE BASIS. A DETERMINATION BY THE GOVERNMENT NOT TO CONDUCT A COMPETITIVE PROCUREMENT, BASED UPON RESPONSES TO THIS NOTICE, IS SOLELY WITHIN THE DISCRETION OF THE GOVERNMENT. ORAL COMMUNICATIONS ARE NOT ACCEPTABLE IN RESPONSE TO THIS NOTICE.

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