Primers and probes

Closed Solicitation Posted

Solicitation number
VEID240021_1301162633
Agency
Responses due
Set-aside
8(a) Sole Source

Opportunity facts

Place of performance
Bellavista Lima 01 Peru

Notice details come from SAM.gov. Updated .

About this opportunity

Written by GovTribe AI ·

The Department of the Navy, Naval Supply Systems Command is seeking to procure customized primers and probes to be used in reverse transcription (RT) real-time PCR assays for the molecular diagnosis of various viruses, including Dengue, Chikungunya, Zika, Orthobunyavirus, Influenza, and SARS-CoV-2. The assays have been validated for use with the TaqMan 7500/7500 Fast Real-Time PCR and QuantStudio platforms at the U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH). The key contract requirements include the specific quantities, catalog numbers, primer and probe sequences, and purification methods needed. The solicitation has a due date of June 20, 2024.

This procurement is set aside for 8(a) small businesses. The items are required to be delivered to the NAMRU SOUTH facility in Bellavista, Peru. The total value of the contract is not provided, but the solicitation includes a detailed list of the required primers and probes, including 246 quantities of P1-2 Primer (Avg. 26 Bases, Salt-Free, 200 nmol), 118 quantities of PPC-2 Primer (with probe purchase, Cartridge Purified, up to 30 bases each, 200 nmol), and various quantities of dual-labeled probes.

Notice text

Primers and probes designed to be used in the molecular diagnosis of Dengue virus (four serotypes), Chikungunya virus, Zika virus, Orthobunyavirus (including Guaroa, Oropouche, Group C, Bunyamwera and California viruses), Influenza virus (Influenza A-matrix gene and B) and its subtypes (H3, H1pdm/SwH1N1, H1 seasonal), and SARS-CoV-2. Primers and probes will be used in reverse transcription (RT) real-time PCR assays using serum samples from febrile surveillance protocol and nasopharyngeal swab samples from the respiratory surveillance protocol.  The items have all been validated and are compatible with the equipment in use at NAMRU SOUTH: 7500 Fast Real-Time PCR and QuantStudio platforms.

  • INFA-FSC2-F1-100 Influenza A Multiplex Fwd Primer 1, 100 nmol - 2 quatities
  • INFA-FSC2-F2-100 Influenza A Multiplex Fwd Primer 2, 100 nmol - 2 quantities
  • INFA-FSC2-R1-100 Influenza A Multiplex Rev Primer 1, 100 nmol - 3 quantities
  • INFA-FSC2-R2-100 Influenza A Multiplex Rev Primer 2, 100 nmol - 1 quantity
  • INFA-FSC2-P-25 Influenza A Multiplex Probe, 25 nmol - 4 quantities
  • INFB-FSC2-F-100 Influenza B Multiplex Fwd Primer, 100 nmol - 4 quantities
  • INFB-FSC2-R-100 Influenza B Multiplex Rev Primer, 100 nmol - 4 quantities
  • INFB-FSC2-P-25 Influenza B Multiplex Probe, 25 nmol - 4 quantities
  • SC2-FSC2-F-100 SARS-CoV-2 Multiplex Fwd Primer, 100 nmol - 4 quantities
  • SC2-FSC2-R-100 SARS-CoV-2 Multiplex Rev Primer, 100 nmol - 4 quantities
  • SC2-FSC2-P-25 SARS-CoV-2 Multiplex Probe, 25 nmol - 4 quantities
  • RNP-F-100 Human RNase P Extraction Control Forward Primer - 100 nmol - 4 quantities
  • RNP-R-100 Human RNase P Extraction Control Reverse Primer - 100 nmol - 4 quantities
  • RNP-FSC2-P-25 Human RNase P Multiplex Probe, 25 nmol - 4 quantities
  • DLO-CAB2-2 Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ -2 200 nmol - 7 quantities
  • DLO-COB1-2 Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1 200 nmol - 5 quantities
  • DLO-FB1-2 Dual-labeled Probe, 5' FAM/3' BHQ-1 200 nmol - 33 quantities
  • DLO-FB1-2sp Dual-labeled Probe, 5' FAM/1 Internal Inosine/3' BHQ-1 200 nmol - 10 quantities
  • DLO-FBP-2 5’ FAM BHQplus Probe Provides 20 nmol delivered - 10 quantities
  • DLO-Q6B2-2 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 200 nmol - 5 quantities
  • LNA-FB1-20 Dual-labeled LNA Probe, 5' FAM/3' BHQ-1 200 nmol - 10 quantities
  • PPC-2 Primer, (with probe purchase) Cartridge Purified (up to 30 bases each) 200 nmol - 118 quantities
  • DNA-2 Custom Oligonucleotide, 21 Bases, 2 Internal Inosines, Cartridge Purified 200 nmol - 5 quantities
  • DNA-2 Custom Oligonucleotide, 22 Bases, 1 Internal Inosine, Cartridge Purified 200 nmol - 5 quantities
  • DNA-2 Custom Oligonucleotide, 22 Bases, 1 Internal Inosine, Cartridge Purified 200 nmol - 5 quantities
  • DNA-2 Custom Oligonucleotide, 22 Bases, 1 Internal Inosine, Cartridge Purified 200 nmol  - 5 quantities
  • DNA-2 Custom Oligonucleotide, 18 Bases, 2 Internal Inosines, Cartridge Purified 200 nmol - 5 quantities
  • DNA-2 Custom Oligonucleotide, 20 Bases, 2 Internal Inosines, Cartridge Purified 200 nmol - 5 quantities
  • PP-2 Primer, (with probe purchase) Salt-Free (up to 30 bases each) 200 nmol - 62 quantities
  • P1-2 Primer, Avg. 26 Bases, Salt-Free 200 nmol - 246 quantities

Files

File Type Posted
VEID240021_SOW.docx DOCX document

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