Low-Coverage Whole Genome Sequencing of Rainbow Trout Samples

The Department of Agriculture Agricultural Research Service seeks low-coverage whole genome sequencing of 2,000 rainbow trout samples via Illumina technology, with quotations due May 26 and questions due May 21 at noon EST. This procurement is a Total Small Business Set-Aside.

Place of performance is Kearneysville, West Virginia. The work encompasses DNA extractions from tissue samples stored in 95% ethanol, 0.5x whole genome sequencing, and return of unused samples. Performance period runs June 1 through September 30, 2026, with sequencing to be completed within 60 days of sample receipt and all deliverables returned within 90 days. Technical requirements specify Illumina PE 150 sequencing with 2x150 nucleotide paired-end reads, minimum 1.2 Gbases output per sample from 8M quality-filtered non-duplicate paired-end reads, and sequence coverage for at least 95% of samples. Deliverables include DNA sequence files in FASTQ format, unused tissue samples, and a comprehensive Final Project Report documenting extraction procedures, quality control measures, library preparations, and sequencing data summaries.

The NAICS code is 541380, Testing Laboratories and Services; PSC is Q301, Reference Laboratory Testing. This is a firm-fixed price requirements contract with three line items: DNA extractions, whole genome sequencing, and return of unused samples. Quotations must include pricing for all three line items and three past performance references relevant to similar work. One award is anticipated. Evaluation follows the Highest Technically Rated with Fair and Reasonable Price method, assessing technical approach, pricing, and past performance. Service Contract Act wage determinations apply to multiple geographic areas (Colorado, California, Idaho, and Maine), though the primary place of performance is West Virginia. Quotations must remain firm for 90 calendar days; F.O.B. destination delivery to Kearneysville is required.