Khufu - Hybrid Capture Genotyping Service

The Department of Agriculture Agricultural Research Service seeks hybrid capture genotyping services for a Sorghum genomics project, with quotations due August 21, 2026, at 2:00 PM Central Time. The Government intends a noncompetitive award to HudsonAlpha Institute for Biotechnology (UEI: M3GAQBN4BWH6) on a single-source basis, citing continuity of a validated workflow jointly developed with USDA-ARS. Questions are due August 19, 2026, at 4:00 PM Central Time.

Place of performance is Cold Spring Harbor, NY. The NAICS code is 541380, Testing Laboratories and Services; PSC is B504, Special Studies/Analysis—Chemical/Biological.

The scope comprises two line items: (1) Khufu hybrid capture genotyping for 120 samples per 96-well plate, including DNA extraction, library preparation, enrichment pooling, sequencing on the Illumina NovaSeq X platform with paired-end 150 bp reads, variant calling, and HapMap-formatted deliverables; and (2) Twist FlexPrep and custom enrichment panel design and printing for 120 samples. Work must use plant-optimized DNA extraction methods to address polysaccharide and polyphenolic content challenges. Deliverables include demultiplexed FASTQ files via secure transfer, HapMap files with imputed genotypes, and a quality control summary report in PDF. Delivery is required within 365 days of award to 1 Bungtown Road, Cold Spring Harbor, NY 11724. This is a firm-fixed-price requirements contract with one-year duration. Award will be made to a single offeror based on lowest price technically acceptable criteria, evaluating technical approach, price, and past performance. Quotations require active SAM.gov registration, acknowledgment of amendments, pricing for all line items, and a technical package demonstrating capability and relevant project experience.