DNA sequencing services

The United States Geological Survey (USGS) Great Lakes Science Center is seeking high throughput, short read sequencing services on the Illumina NovaSeq X platform to generate whole genome data for native salmonids in the Great Lakes. The procurement requires quality control analysis, sequencing services, and delivery of demultiplexed sequence data from genomic DNA libraries prepared by the USGS Molecular Ecology Lab's facilities. This Request for Quotations (RFQ 140G0225Q0111) falls under NAICS code 541380 (Testing Laboratories and Services) and will be evaluated using FAR Part 12 commercial acquisition procedures in conjunction with FAR Part 13.1 simplified procedures. Vendors must provide a two-page technical capabilities summary and be registered in the System for Award Management (SAM) prior to submission. The deadline for electronic quotations is September 5, 2025, at 2:30 p.m. Mountain Daylight Time, with responses to vendor questions provided through an amendment issued on August 28, 2025.

This solicitation is not designated as a small business set-aside and has a size standard of $19.0 million for the Testing Laboratories and Services category. The contract involves sequencing services for two master pre-made libraries containing 89 individuals total, requiring 4 lanes of PE150 sequencing with a 25B flow cell for each library, which can be consolidated across one entire 8-lane flow cell due to unique dual-index combinations. The period of performance extends from September 15, 2025, to March 20, 2026, with the contract anticipated to be awarded as a firm-fixed-price agreement. The work specifically requires the use of the SRSLY NGS Library Prep Kit for Illumina NanoPlus Uracil+ and mandates the Illumina platform to prevent platform-based batch effects in the genomic analysis. Performance will involve secure data delivery of the sequenced genomic information for the Great Lakes native salmonid research project.