Detection of global proteins and mRNA in postnatal heart targeted AdCy8 overexpression mouse strain

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Background:  The overexpression of adenylyl cyclase in the mouse heart produces a strong beating heart with the upregulation of many pathways. Global analysis of proteins that emerge are central for the heart survival and regulation of the cell cycle and mitosis check points.

NIA plans to scan global proteins and mRNA by detecting its binding partners in the AdCy8 overexpression model. This analysis will be done through the use of a special method called immunoprecipitation (IP) followed by mass spectrometry analysis (MS), mRNA seq and comparison of the results with the wild type mouse model.

Purpose and Objectives: The purpose of this project is to study postnatal protein expression and mRNA signals in AC8 models in order to illucidate their pathways and their mechanisms in heart cell survival, cell renewal and cell mitosis and its regulation.

Project requirements: Method development and testing of Immuno precipitation (IP) (24 samples), MS analysis (16 samples) and mRNA seq (24 samples). The requirement for IP-LC/MS is to provide biochemical identification, relative quantification, and statistical analysis with data visualizationfor each sample. mRNA seq requirement is to provide multiomicsassociations with numerous analytes that are distributed across the different biochemical classes including small molecules, lipids and proteins,using our small sample size, demonstrating high signal-to-noise ratios.

Anticipated period of performance: Deliverables must be received within 90 days after receipt of samples.

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Data Rights: All Data produced will be the sole property of NIH

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