Automated Molecular Biology Workstation

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6 as supplemented with additional information included in this notice. Solicitation number is 12405B20Q0085 is issued as a Request for Quotation (RFQ). This announcement constitutes the only solicitation; quotations are being requested and a written solicitation will not be issued.. The associated North American Industrial Classification System (NAICS) code for this procurement is 334516 Analytical Laboratory Instrument Manufacturing 1,000

 The USDA, Agricultural Research Service (ARS) South East Area (SEA)  Horticulture Research Lab located in  Fort Pierce, Florida 34945 has a requirement for: Quantity of one (1) An automated molecular biology workstation optimized for one-step running of Gibson Assembly-based gene synthesis from commercial DNA synthesis kits purchased from Codex-DNA, Inc. Optimized for: generating clones, DNA variant libraries, gene fragments, genes, and genomes, and be able to automate next-generation sequencing (NGS) library preparation protocols.

Must have the following features:

1. An automated workstation optimized for: generating clones, DNA variant libraries, gene fragments, genes, and genomes, and be able to automate next-generation sequencing (NGS) library preparation protocols.
2. Ability to build gene fragments, genes, genomes, clones, DNA variant libraries, and perform NGS library preparation on the same platform without additional user programming.
3. Ability to generate up to 32 high-quality individual dsDNA fragments in under 24 hours, using a standard 96-well plate.
4. Ability to generate up to 10 μg of DNA material ranging in size from 300 bp to 3,600 bp.
5. Ability to be optimized for one-step support of Codex DNA unique master mixes.
6. Ability to perform a proprietary two-step error correction process, resulting in low error rates of up to 1:30,000 bp.
7. Ability to automatically generate clones via insertion of constructs into user-specified or off-the-shelf vectors using Gibson Assembly® method.
8. Ability to automatically scan barcodes from reagent modules to download job-specific, pre-validated scripts directly to the instrument for hands-free operation.
9. Ability to be able to be locally controlled via onboard software without the need for a separate standalone computer.
10. A fully integrated onboard thermocycler used in both isothermal and temperature gradient applications.
11. Ability to allow simplified execution of the following steps: Submit sequence or DNA variant library of interest to Codex DNA and then allow Codex modules, which are shipped to us, to be inserted and rapidly initiate stand-alone synthesis process without interruption.

Shipping and Handling to FOB

Contractor shall deliver the required supplies in a fully functional state to include (all materials, equipment, supervision, transportation, labor, installation, and training).

Required delivery date 10/15/2020

Delivery is F.O.B. Destination (shipping and handling included)

Delivery must state door to door to the following address:

USDA-ARS-SEA

 HORTICULTURE RESEARCH LAB

2001 S. ROCK ROAD

FORT PIERCE, FLORIDA 34945

ATTN: Belkis Diego

Awarded contract 12405B20P0510 to CODEXDNA INC for 77000.00 on 2020-09-19.