specifications for automated workstation requirements-8-4-2020.pdf

Requirement for automated workstation that is compatible with Codex-DNA clone synthesis kits that we use in our research.

Title of the system: An automated molecular biology workstation optimized for one-step running of Gibson Assembly-based gene synthesis from commercial DNA synthesis kits purchased from Codex-DNA, Inc.

Optimized for: generating clones, DNA variant libraries, gene fragments, genes, and genomes, and be able to automate next-generation sequencing (NGS) library preparation protocols.

The system must have:

1. An automated workstation optimized for: generating clones, DNA variant libraries, gene fragments, genes, and genomes, and be able to automate next-generation sequencing (NGS) library preparation protocols.

2. Ability to build gene fragments, genes, genomes, clones, DNA variant libraries, and perform NGS library preparation on the same platform without additional user programming.

3. Ability to generate up to 32 high-quality individual dsDNA fragments in under 24 hours, using a standard 96-well plate.

4. Ability to generate up to 10 μg of DNA material ranging in size from 300 bp to 3,600 bp.

5. Ability to be optimized for one-step support of Codex DNA unique master mixes.

6. Ability to perform a proprietary two-step error correction process, resulting in low error rates of up to 1:30,000 bp.

7. Ability to automatically generate clones via insertion of constructs into user-specified or off-the-shelf vectors using Gibson Assembly® method.

8. Ability to automatically scan barcodes from reagent modules to download job-specific, pre-validated scripts directly to the instrument for hands-free operation.

9. Ability to be able to be locally controlled via onboard software without the need for a separate standalone computer.

10. A fully integrated onboard thermocycler used in both isothermal and temperature gradient applications.

11. Ability to allow simplified execution of the following steps: Submit sequence or DNA variant library of interest to Codex DNA and then allow Codex modules, which are shipped to us, to be inserted and rapidly initiate stand-alone synthesis process without interruption.